Planer Kryo550-16 User manual

Kryo550-16
Operator’s Manual

© 2013 Planer plc
Planer plc
110 Windmill Road
Sunbury-on-Thames
Middlesex
U.K.
TW16 7HD
Telephone: [phone]
Facsimile: [phone]
Warnings:
Never leave the freezer unattended when running a program.
Take care to avoid placing chambers in environments influenced by sources of
electromagnetic interference, such as cyclotrons, large transformers etc.
This equipment is not suitable for operation in explosive atmospheres.
Kryo550-16 Operator's Manual
MA009149
Issue 01 - July 2003
Issue 02 - August 2003
Issue 03 - November 2003
Issue 04 - February 2004
Issue 05 - May 2008
Issue 06 - December 2009
Issue 07 - August 2013
Microsoft is a registered trademark, and Windows is a trademark of Microsoft Corporation in
the United States of America and other countries.
Notices
This equipment contains batteries and other components which have a limited life. To ensure
the long life of the equipment and help ensure trouble-free operation, we recommend that
you join one of our Maintenance and Support Schemes. Please contact your supplier for
details.
Good practice demands that in critical applications, reliance should not be placed on a single
source of data. Hard copies and backup copies should be routinely kept.
All equipment supplied by Planer plc. is subject to a Warranty Agreement and Limited
Liability. In addition, software products are not sold but are licensed for use and are subject
to a Licence Agreement. Copies of these documents will have been provided with the
equipment or as part of the software installation. Please ensure that you read these documents
before using the equipment or software. If you cannot locate this documentation, please
contact your supplier who will be able to provide you with copies.
Planer plc reserve the right to alter products and their specifications without notice

Symbols Used in this Manual
Failure to comply with these instructions could result in personal injury.
Failure to follow these instructions could result in damage to your equipment,
system or samples.
This indicates first aid advice.
Safety i

Symbols Used on the Chamber
Run key
Stop key
Heat manual override
Cool manual override
Mains power off
Mains power on
Sample display key
Caution - Read this manual. Failure to observe the
instructions within this manual could result in personal
injury.
!
Alarm output
Reset
Sample PRT
Chamber PRT
Alternating current (AC)
Ethernet 10 Base T
RS232 serial port
ii Safety

SAFETY PRECAUTIONS
IT IS IMPORTANT TO READ THIS SAFETY NOTICE PRIOR TO OPERATING ANY
EQUIPMENT
First Aid
IF IN DOUBT, SEEK IMMEDIATE MEDICAL ATTENTION. If any
quantity of liquid nitrogen comes into contact with the skin or eyes,
immediately flood that area of the body with large quantities of unheated
water, apply cold compresses and seek medical attention. If the skin is
blistered or there is any possibility that eyes have been affected, the patient
should be taken immediately to a doctor or hospital for treatment.
Safety
Failure to comply with these instructions could result in personal
injury
Operating the system in a manner not specified within in this manual or under conditions
outside of the specifications, see Appendix A, may result in the protection offered by the
equipment being impaired.
Avoid nitrogen build up: anywhere liquid nitrogen is used must be well ventilated to
reduce the risk of nitrogen build up. Small volumes of liquid nitrogen convert to very
large volumes of gas. This can result in drowsiness, or in extreme cases, asphyxiation.
When liquid nitrogen is used in confined spaces, oxygen-deficiency alarms must be
installed. Guidance on ventilation requirements are given in BCGA Code of Practice
CP30, The safe use of liquid nitrogen dewars up to 50 litres.
When there is a risk of reduced or insufficient ventilation when a room is unoccupied,
then alarms to indicate oxygen deficiency must be installed and positioned outside of the
room so that operators are aware of the hazard before entering. The operational status of
such an alarm should also be visible from outside the room.
If liquid nitrogen is used in an area that requires forced ventilation, an alarm to indicate
its failure should be fitted.
When dispensing or potentially being exposed to liquid nitrogen, protect the face with a
shield and wear gloves, boots and a protective apron. Prevent spillage into shoes and onto
unprotected parts of the body.
Handle vessels containing liquid nitrogen carefully. Liquid nitrogen boils at -196ºC and
both liquid and gas can cause rapid and severe frostbite. Delicate tissue, e.g. the eyes, can
be damaged by an exposure to the cold gas, which may be too brief to affect the skin of
the hands or face.
Stand clear of boiling and splashing liquid nitrogen and its gas when filling a dewar at
normal room temperature or when inserting objects (such as a pump) into the liquid.
Use tongs or wear cryogloves when handling cold or hot objects. Cryogloves are available
as an accessory from Planer Biomed. When running a program, the chamber and contents
may get cold enough to cause frostbite.
The dewar must be depressurised before the pump can be removed. This is achieved by
opening the pressure-release valve mounted on the side of the pump, which seals an
Safety iii

orifice in the pressure system by toggle action. Avoid contact with cold nitrogen gas when
depressurising the dewar.
The dewars used with this equipment must never be used to store any liquid other than
liquid nitrogen.
Only use vessels designed for working with liquid nitrogen.
Secure the liquid nitrogen container to prevent toppling.
Ensure that the delivery pipe connections are secure and leak-free before supplying liquid
nitrogen to the freezer.
Liquid nitrogen pressure must not exceed 2.0 bar (30 psi).
To maintain protection against electric shock, the mains lead must be properly fitted to a
3-way mains connector plugged into a mains outlet providing a protective earth
connection.
Because of the potentially wet laboratory environment, the operator should be provided
with additional protection against electric shock by supplying mains power to the freezer,
pump, local computer (if connected) and auxiliary equipment through a residual current
circuit breaker (RCCB) operating at a differential of 30 mA. Note that care must be taken
to ensure that the freezer is not compromised by faulty equipment sharing the same supply
and causing nuisance trips.
The 9-way D Type plug on the Kryo550-16 interface panel is only for connecting to the
RS232 serial port of a computer which complies with IEC950.
The RJ45 ethernet connector provided with the optional kryo-connect card is only for
connection to a Network Interface Card (NIC) or Network Hub capable of supporting 10
Base T. The equipment to which the Kryo550-16 is connected must comply with
IEC950.
The Sample PRT socket on the Kryo550-16 interface panel is only to be used with a
Planer Sample PRT.
When heating under manual control, check the chamber temperature at intervals of 30
seconds or less to avoid overheating.
User servicing is limited to cleaning. All other servicing must only be undertaken by
suitably qualified engineers.
To avoid risk of fire, fuses must always be replaced with the same type and rating.
The Kryo550-16 weighs 29 kg and requires at least two people for safe handling. Do not
attempt to lift using the edges of the case but use the bottom chassis plate.
When switched on but not running a profile, the Kryo550-16 chamber and contents may
eventually become hot enough to cause burns.
The pipework and delivery hose at the rear of the freezer will become extremely cold and
touching these parts can result in severe frostbite.
Do not move the Kryo550-16 whilst still wet after a freezing run.
iv Safety

EMC and ESD PRECAUTIONS
The following precautions must be taken to ensure that the equipment is not
damaged by electrostatic discharge (ESD), and that its immunity to radio
frequency interference is not compromised.
Ensure that the Kryo550-16 is not running a program and that the mains power is
disconnected before connecting or disconnecting any cable.
Fit the plastic covers and plugs provided to all unused connectors.
Immediately before touching the sample PRT, touch an earthed part, such as the metal
surface at the rear of the Kryo550-16.
Do not touch any uncovered connectors with your hands or tools, even when the
equipment is switched off.
When connecting the system to a PC via the 9 way plug, always use a fully screened cable
no longer than 2m.
Take care to avoid placing freezers in environments influenced by sources of
electromagnetic interference, such as cyclotrons, large transformers etc.
Safety v

Contents
C-1
Appendix C Safety Declaration
............................
..............
B-1AlarmOutput ..........................................................
B-1
Appendix B Alarm Output .............................................
A-2Batteries ..............................................................
A-2Fuses ................................................................
A-1SystemSpecifications ...................................................
A-1
Appendix A Specifications ..............................................
4-3Disposal ...............................................................
4-3ReturningforService .....................................................
4-2Troubleshooting .........................................................
4-1RoutineMaintenance .....................................................
4-1
Routine Maintenance and Troubleshooting .................................
4-1
Chapter 4 Routine Maintenance and Troubleshooting .........................
3-5
 To deal with a system failure ..........................................
3-5  The run has stopped prematurely ......................................
3-5Dealing with a System Failure During a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3-5PowerFailures ..........................................................
3-3AboutSeeding ..........................................................
3-2SwitchingofftheSystem ..................................................
3-2  If the run was started from DeltaT .....................................
3-2  If the run was started from the control panel .............................
3-2WarmingtheChamber ....................................................
3-2RemovingtheSamples ...................................................
3-1LoadingtheSamples .....................................................
3-1StartingtheRun .........................................................
3-1
Chapter 3 Freezing Samples ..............................................
2-3Installing a Planer Pump and Dewar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2-2Installing a CryoCyl Low Pressure Cylinder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2-1
Chapter 2 Installing the Kryo550-16 .......................................
1-7Kryo550-16ProfileMemory ...............................................
1-7LiquidNitrogenDeliverySystem ...........................................
1-6Printer ................................................................
1-6PersonalComputer(PC) ..................................................
1-6Kryo550-16Features .....................................................
1-5RearPanel ............................................................
1-4InterfacePanel ..........................................................
1-3ControlPanel ...........................................................
1-2TypicalInstallation ......................................................
1-1SystemComponents .....................................................
1-1
Chapter 1 Introduction to the Kryo550-16 ..................................
viContents ................................................................
vEMCPRECAUTIONS ....................................................
iiiSafety ..................................................................
iiiFirstAid ...............................................................
iii
SAFETY PRECAUTIONS ................................................
iiSymbolsUsedontheChamber ..............................................
iSymbolsUsedinthisManual ...............................................
vi Contents

C-1
Safety Declaration .....................................................
Contents vii

viii Contents

Chapter 1 Introduction to the Kryo550-16
Welcome to the Kryo550-16, a fully featured tissue bank freezer for the cryopreservation of
bone marrow, stem cells, skin, cord blood and other critical high volume samples.
This manual is intended for both operators of the freezer and those persons responsible for its
installation.
System Components
The major components of the system are shown below.
Introduction to the Kryo550-16 1-1
Control panel Pressure relief valveChamber
Cable tidyInterface panel

Typical Installation
1-2 Introduction to the Kryo550-16
Mains power
Personal
computer
Printer
Ethernet hub
Alternative network connection if
kryo-connect card fitted
Pump power supply
LNP4 pump
and dewar

Control Panel
Introduction to the Kryo550-16 1-3
Run indicator light
Illuminated when running a profile.
Flashes for operator attention.
Run button
Press to start a profile
Stop button
Press to stop a profile
Cool indicator light
Illuminated when cooling.
Heat indicator light
Illuminated when heating. Power indicator
Illuminated when mains power
is switched on.
Sample key
Keep depressed to
display the sample
temperature
Normally shows chamber temperature.
While the Sample key is pressed, the display
shows the sample temperature.
Display

Interface Panel
1-4 Introduction to the Kryo550-16
Reset button
Manual override - heat
Manual override - cool
Sample PRT
Alarm output
RS232 Serial port
kryo-connect ethernet connector
(if fitted)

Rear Panel
Introduction to the Kryo550-16 1-5
Mains on/off
switch
Pressure
relief
valve
Mains power
inlet
Liquid nitrogen
inlet

Kryo550-16 Features
Precision temperature control
Accurate linear heating and cooling is achieved by the pulsing of a heater and controlled
injection of atomised liquid nitrogen into the chamber.
Precision temperature monitoring
The chamber temperature is measured by a platinum resistance thermometer (PRT).
Stainless steel construction
The chamber is made from high-quality stainless steel for durability and cleanliness.
Programmable control
The operation is controlled by a microprocessor based system. The controller stores the
profile that specifies start temperature, step rates etc. in non-volatile memory. The profile can
be retained in memory for several years, even if power to the Kryo550-16 is disconnected.
The profile can be changed by using the DeltaT™ software on a personal computer (PC).
Stand-alone operation
The Kryo550-16 can store a profile that can run independently of the personal computer (PC).
See Kryo550-16 Profile Memory
Heat / Cool switches
Manual override push button switches on the interface panel allow manual control of the
heating and cooling functions.
Personal Computer (PC)
The DeltaT ™ or DeltaT-iQ ™ software on a PC enables you to create and store profiles.
You can run these profiles from the PC and view the recorded chamber and sample
temperatures graphically. All results from the run are stored on the PC for traceability
purposes.
Any profile you run from the PC may be selected to be stored in the Kryo550-16's memory
(overwriting any previously stored profile). This enables you to rerun the profile directly
from the freezer without having the PC connected. See Kryo550-16 Profile Memory.
Printer
If you are using a PC, it is strongly recommended that you connect a printer. You can use the
printer for such tasks as printing profiles and graphs.
1-6 Introduction to the Kryo550-16

Liquid Nitrogen Delivery System
Never connect to a supply pressure in excess of 2 bar (30 psi)
For optimum performance the Kryo550-16 should be configured for the
supply pressure to be used. Two ranges are supported: 0.34 to 0.53 bar (5
to 7.7 psi) and 1.38 to 1.65 bar (20 - 24 psi). Please contact your
distributor if your system needs to be reconfigured. Pressures outside of
these ranges may compromise the performance of the system.
It is normal to use a 1.5 bar (22 psi) EuroCyl or CryoCyl cylinder to provide the supply of
liquid nitrogen to the oven. The cylinder is filled by the supplier and, being self-pressurising,
does not require a pump to deliver the liquid nitrogen to the chamber.
The system can be configured for left or right entry of the hose. Please contact your service
engineer if you want the hose configuration changed.
Kryo550-16 Profile Memory
The Kryo550-16 has two memory locations that are used for storing profiles. These are the
Permanent Profile and Run Profile areas. When the Run button on the control panel is used
to start a profile, the freezing profile stored in the Permanent Profile memory is copied into
the Run Profile location and then started. If a profile is started from DeltaT, the profile from
the PC is sent directly to the Run Profile area and started. The Permanent Profile is left
untouched. This means that after running a profile from DeltaT, the original profile in the
Permanent Profile memory will still be available for use by pressing the Run button.
DeltaT can be directed to overwrite the Permanent Profile memory by selecting the Make
Permanent option from the PC when running the profile. Refer to the DeltaT help files for
further information.
Introduction to the Kryo550-16 1-7

1-8 Introduction to the Kryo550-16

Chapter 2 Installing the Kryo550-16
The Kryo550-16 freezer weighs 25 kg and requires at least two people for
safe handling. Do not attempt to lift using the edges of the case but curl your
fingers underneath to support the bottom chassis plate.
Carefully unpack the equipment.
Place the Kryo550-16 on a secure, flat surface.
Connect the sample platinum resistance thermometer (PRT) into the PRT socket on the
interface panel.
The RS232 serial port (9-way plug) on the Kryo550-16 is only for
connecting to the serial port of a computer which complies with IEC950
When connecting the system to a PC via the 9 way plug, always use a fully
screened cable no longer than 2m.
If you do not have the kryo-connect option and want to interface with a PC, connect
RS232 serial port connector ( 9 way D type plug) on the side of the Kryo550-16 to an
RS232 port on the PC using the cable supplied. This is a straight-through one to one
cable. A null-modem cable must not be used.
The Ethernet is only for connection to a Network Interface Card (NIC) or
Network Hub capable of supporting 10 Base T. The equipment to which the
Kryo550-16 is connected must comply with IEC950. The unit should only
be connected to an Ethernet LAN internal to the building.
If you have the kryo-connect option installed, connection to a PC must be made through
the Ethernet connection. In this case, follow the instructions in the kryo-connect guide.
Ensure that the ventilation requirements described in the Safety Precautions
section of this guide have been fully met.
If using a Planer pump and dewar refer to the instructions under Installing a Planer
Pump and Dewar or if using a CryoCyl low pressure cylinder, refer to the instructions
under Installing a CryoCyl Low Pressure Cylinder.
To maintain protection against electric shock, the mains lead must be
properly fitted to a 3-way mains connector plugged into a mains outlet
providing a protective earth connection.
Because of the potentially wet laboratory environment, the operator should
be provided with additional protection against electric shock by supplying
mains power to the freezer through a residual current circuit breaker (RCCB)
operating at a differential of 30 mA. Note that care must be taken to ensure
that the freezer is not compromised by faulty equipment sharing the same
supply and causing nuisance trips.
After connecting the liquid nitrogen supply, connect the mains inlet at the rear of the
Kryo550-16, to a suitable wall socket.
Switch on the power to the Kryo550-16 and the pump (if fitted). The power indicator on
the control panel will illuminate.
Installing the Kryo550-16 2-1

Installing a CryoCyl Low Pressure Cylinder
Wear protective clothing, including cryogloves, goggles, boots and a
protective apron.
The liquid-nitrogen supply pressure must not exceed 2.0 bar (30 psi).
If the hose is not already fitted to the Kryo550-16, wrap the male hose thread with PTFE
tap and fix to the LN
2
input on the back of the chamber. Tighten securely with a spanner.
Connect an appropriate adapter to the cylinder, observing the manufacturer’s instructions,
then connect the delivery tube to the adapter ensuring that the fibre washer is fitted to this
joint. Tighten securely with a spanner.
After connecting both ends of the delivery tube, open the liquid-nitrogen tap
slowly and check for leaks.
2-2 Installing the Kryo550-16