Planer Kryo550-16 User manual

Kryo550-16
Operator’s Manual

© 2013 Planer plc
Planer plc
110 Windmill Road
Sunbury-on-Thames
Middlesex
U.K.
TW16 7HD
Telephone: [phone]
Facsimile: [phone]
Warnings:
Never leave the freezer unattended when running a program.
Take care to avoid placing chambers in environments influenced by sources of
electromagnetic interference, such as cyclotrons, large transformers etc.
This equipment is not suitable for operation in explosive atmospheres.
Kryo550-16 Operator's Manual
MA009149
Issue 01 - July 2003
Issue 02 - August 2003
Issue 03 - November 2003
Issue 04 - February 2004
Issue 05 - May 2008
Issue 06 - December 2009
Issue 07 - August 2013
Microsoft is a registered trademark, and Windows is a trademark of Microsoft Corporation in
the United States of America and other countries.
Notices
This equipment contains batteries and other components which have a limited life. To ensure
the long life of the equipment and help ensure trouble-free operation, we recommend that
you join one of our Maintenance and Support Schemes. Please contact your supplier for
details.
Good practice demands that in critical applications, reliance should not be placed on a single
source of data. Hard copies and backup copies should be routinely kept.
All equipment supplied by Planer plc. is subject to a Warranty Agreement and Limited
Liability. In addition, software products are not sold but are licensed for use and are subject
to a Licence Agreement. Copies of these documents will have been provided with the
equipment or as part of the software installation. Please ensure that you read these documents
before using the equipment or software. If you cannot locate this documentation, please
contact your supplier who will be able to provide you with copies.
Planer plc reserve the right to alter products and their specifications without notice

Symbols Used in this Manual
Failure to comply with these instructions could result in personal injury.
Failure to follow these instructions could result in damage to your equipment,
system or samples.
This indicates first aid advice.
Safety i

Symbols Used on the Chamber
Run key
Stop key
Heat manual override
Cool manual override
Mains power off
Mains power on
Sample display key
Caution - Read this manual. Failure to observe the
instructions within this manual could result in personal
injury.
!
Alarm output
Reset
Sample PRT
Chamber PRT
Alternating current (AC)
Ethernet 10 Base T
RS232 serial port
ii Safety

SAFETY PRECAUTIONS
IT IS IMPORTANT TO READ THIS SAFETY NOTICE PRIOR TO OPERATING ANY
EQUIPMENT
First Aid
IF IN DOUBT, SEEK IMMEDIATE MEDICAL ATTENTION. If any
quantity of liquid nitrogen comes into contact with the skin or eyes,
immediately flood that area of the body with large quantities of unheated
water, apply cold compresses and seek medical attention. If the skin is
blistered or there is any possibility that eyes have been affected, the patient
should be taken immediately to a doctor or hospital for treatment.
Safety
Failure to comply with these instructions could result in personal
injury
Operating the system in a manner not specified within in this manual or under conditions
outside of the specifications, see Appendix A, may result in the protection offered by the
equipment being impaired.
Avoid nitrogen build up: anywhere liquid nitrogen is used must be well ventilated to
reduce the risk of nitrogen build up. Small volumes of liquid nitrogen convert to very
large volumes of gas. This can result in drowsiness, or in extreme cases, asphyxiation.
When liquid nitrogen is used in confined spaces, oxygen-deficiency alarms must be
installed. Guidance on ventilation requirements are given in BCGA Code of Practice
CP30, The safe use of liquid nitrogen dewars up to 50 litres.
When there is a risk of reduced or insufficient ventilation when a room is unoccupied,
then alarms to indicate oxygen deficiency must be installed and positioned outside of the
room so that operators are aware of the hazard before entering. The operational status of
such an alarm should also be visible from outside the room.
If liquid nitrogen is used in an area that requires forced ventilation, an alarm to indicate
its failure should be fitted.
When dispensing or potentially being exposed to liquid nitrogen, protect the face with a
shield and wear gloves, boots and a protective apron. Prevent spillage into shoes and onto
unprotected parts of the body.
Handle vessels containing liquid nitrogen carefully. Liquid nitrogen boils at -196ºC and
both liquid and gas can cause rapid and severe frostbite. Delicate tissue, e.g. the eyes, can
be damaged by an exposure to the cold gas, which may be too brief to affect the skin of
the hands or face.
Stand clear of boiling and splashing liquid nitrogen and its gas when filling a dewar at
normal room temperature or when inserting objects (such as a pump) into the liquid.
Use tongs or wear cryogloves when handling cold or hot objects. Cryogloves are available
as an accessory from Planer Biomed. When running a program, the chamber and contents
may get cold enough to cause frostbite.
The dewar must be depressurised before the pump can be removed. This is achieved by
opening the pressure-release valve mounted on the side of the pump, which seals an
Safety iii

orifice in the pressure system by toggle action. Avoid contact with cold nitrogen gas when
depressurising the dewar.
The dewars used with this equipment must never be used to store any liquid other than
liquid nitrogen.
Only use vessels designed for working with liquid nitrogen.
Secure the liquid nitrogen container to prevent toppling.
Ensure that the delivery pipe connections are secure and leak-free before supplying liquid
nitrogen to the freezer.
Liquid nitrogen pressure must not exceed 2.0 bar (30 psi).
To maintain protection against electric shock, the mains lead must be properly fitted to a
3-way mains connector plugged into a mains outlet providing a protective earth
connection.
Because of the potentially wet laboratory environment, the operator should be provided
with additional protection against electric shock by supplying mains power to the freezer,
pump, local computer (if connected) and auxiliary equipment through a residual current
circuit breaker (RCCB) operating at a differential of 30 mA. Note that care must be taken
to ensure that the freezer is not compromised by faulty equipment sharing the same supply
and causing nuisance trips.
The 9-way D Type plug on the Kryo550-16 interface panel is only for connecting to the
RS232 serial port of a computer which complies with IEC950.
The RJ45 ethernet connector provided with the optional kryo-connect card is only for
connection to a Network Interface Card (NIC) or Network Hub capable of supporting 10
Base T. The equipment to which the Kryo550-16 is connected must comply with
IEC950.
The Sample PRT socket on the Kryo550-16 interface panel is only to be used with a
Planer Sample PRT.
When heating under manual control, check the chamber temperature at intervals of 30
seconds or less to avoid overheating.
User servicing is limited to cleaning. All other servicing must only be undertaken by
suitably qualified engineers.
To avoid risk of fire, fuses must always be replaced with the same type and rating.
The Kryo550-16 weighs 29 kg and requires at least two people for safe handling. Do not
attempt to lift using the edges of the case but use the bottom chassis plate.
When switched on but not running a profile, the Kryo550-16 chamber and contents may
eventually become hot enough to cause burns.
The pipework and delivery hose at the rear of the freezer will become extremely cold and
touching these parts can result in severe frostbite.
Do not move the Kryo550-16 whilst still wet after a freezing run.
iv Safety

EMC and ESD PRECAUTIONS
The following precautions must be taken to ensure that the equipment is not
damaged by electrostatic discharge (ESD), and that its immunity to radio
frequency interference is not compromised.
Ensure that the Kryo550-16 is not running a program and that the mains power is
disconnected before connecting or disconnecting any cable.
Fit the plastic covers and plugs provided to all unused connectors.
Immediately before touching the sample PRT, touch an earthed part, such as the metal
surface at the rear of the Kryo550-16.
Do not touch any uncovered connectors with your hands or tools, even when the
equipment is switched off.
When connecting the system to a PC via the 9 way plug, always use a fully screened cable
no longer than 2m.
Take care to avoid placing freezers in environments influenced by sources of
electromagnetic interference, such as cyclotrons, large transformers etc.
Safety v

Contents
C-1
Appendix C Safety Declaration
............................
..............
B-1AlarmOutput ..........................................................
B-1
Appendix B Alarm Output .............................................
A-2Batteries ..............................................................
A-2Fuses ................................................................
A-1SystemSpecifications ...................................................
A-1
Appendix A Specifications ..............................................
4-3Disposal ...............................................................
4-3ReturningforService .....................................................
4-2Troubleshooting .........................................................
4-1RoutineMaintenance .....................................................
4-1
Routine Maintenance and Troubleshooting .................................
4-1
Chapter 4 Routine Maintenance and Troubleshooting .........................
3-5
 To deal with a system failure ..........................................
3-5  The run has stopped prematurely ......................................
3-5Dealing with a System Failure During a Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
3-5PowerFailures ..........................................................
3-3AboutSeeding ..........................................................
3-2SwitchingofftheSystem ..................................................
3-2  If the run was started from DeltaT .....................................
3-2  If the run was started from the control panel .............................
3-2WarmingtheChamber ....................................................
3-2RemovingtheSamples ...................................................
3-1LoadingtheSamples .....................................................
3-1StartingtheRun .........................................................
3-1
Chapter 3 Freezing Samples ..............................................
2-3Installing a Planer Pump and Dewar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2-2Installing a CryoCyl Low Pressure Cylinder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
2-1
Chapter 2 Installing the Kryo550-16 .......................................
1-7Kryo550-16ProfileMemory ...............................................
1-7LiquidNitrogenDeliverySystem ...........................................
1-6Printer ................................................................
1-6PersonalComputer(PC) ..................................................
1-6Kryo550-16Features .....................................................
1-5RearPanel ............................................................
1-4InterfacePanel ..........................................................
1-3ControlPanel ...........................................................
1-2TypicalInstallation ......................................................
1-1SystemComponents .....................................................
1-1
Chapter 1 Introduction to the Kryo550-16 ..................................
viContents ................................................................
vEMCPRECAUTIONS ....................................................
iiiSafety ..................................................................
iiiFirstAid ...............................................................
iii
SAFETY PRECAUTIONS ................................................
iiSymbolsUsedontheChamber ..............................................
iSymbolsUsedinthisManual ...............................................
vi Contents

C-1
Safety Declaration .....................................................
Contents vii

viii Contents