Waters ACQUITY Arc System Parts list manual

ACQUITY Arc
System Guide
715004747 / Revision A
Copyright © Waters Corporation 2015
All rights reserved

General information
Copyright notice
© 2015 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA
AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF
MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF
THE PUBLISHER.
Trademarks
ACQUITY® is a registered trademark of Waters Corporation.
ACQUITY ArcTM is a trademark of Waters Corporation.
Auto•Blend PlusTM is a trademark of Waters Corporation.
ACQUITY QDaTM is a trademark of Waters Corporation.
Connections INSIGHT® is a registered trademark of Waters Corporation.
CORTECSTM is a trademark of Waters Corporation.
Empower® is a registered trademark of Waters Corporation.
LAC/ETM is a trademark of Waters Corporation.
MassLynx® is a registered trademark of Waters Corporation.
TORX® is a registered trademark of Camcar Division, Textron Inc.
Tygon® is a registered trademark of Saint-Gobain Performance Plastics Corporation.
PHILLIPS® is a registered trademark of Phillips Screw Company.
THE SCIENCE OF WHAT'S POSSIBLE® is a registered trademark of Waters
Corporation.
Customer comments
Waters’ Technical Communications organization invites you to report any errors that
you encounter in this document or to suggest ideas for otherwise improving it. Help
us better understand what you expect from our documentation so that we can
continuously improve its accuracy and usability.
We seriously consider every customer comment we receive. You can reach us at
[email protected].
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Contacting Waters
Contact Waters with enhancement requests or technical questions regarding the use,
transportation, removal, or disposal of any Waters product. You can reach us via the
Internet, telephone, or conventional mail.
Waters contact information
Contacting
medium
Information
Internet The Waters Web site includes contact information for Waters
locations worldwide.
Visit www.waters.com
Telephone and fax From the USA or Canada, phone [phone], or fax
508-872-1990.
For other locations worldwide, phone and fax numbers appear
in the Waters Web site.
Conventional mail Waters Corporation
Global Support Services
34 Maple Street
Milford, MA 01757
USA
Safety considerations
Some reagents and samples used with Waters instruments and devices can pose
chemical, biological, or radiological hazards (or any combination thereof). You must
know the potentially hazardous effects of all substances you work with. Always follow
Good Laboratory Practice (GLP), and consult your organization’s standard operating
procedures as well as your local requirements for safety.
Safety hazard symbol notice
Documentation needs to be consulted in all cases where the symbol is used to
find out the nature of the potential hazard and any actions which have to be taken.
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Power cord replacement hazard
Warning: To avoid electric shock, use the SVT-type power cord in the
United States and HAR-type (or better) cord in Europe. The main power cord
must be replaced only with one of adequate rating. For information
regarding what cord to use in other countries, contact your local Waters
distributor.
Hand crush hazard
Warning: To avoid hazards associated with the reciprocating or rotating
parts in the source, keep hands clear of the regions marked with yellow and
gray labels.
High voltage hazard
Warning: To avoid electric shock, observe these precautions:
• Do not remove the mass spectrometer’s protective panels. The
components they cover are not user-serviceable.
• When the instrument is in Operate mode, avoid touching the areas
marked with the high voltage warning symbol. To touch external areas
marked with the symbol, first put the instrument in Standby mode.
Bottle placement prohibition
Prohibited: To avoid injury from electrical shock or fire, and damage to the
equipment, do not place vessels containing liquid atop the workstation or ancillary
equipment or otherwise expose those units to dripping or splashing liquids.
Prohibited: Do not place vessels containing liquid—such as solvent bottles
—atop the workstation or ancillary equipment or otherwise expose those
units to dripping or splashing liquids.
FCC radiation emissions notice
Changes or modifications not expressly approved by the party responsible for
compliance, could void the users authority to operate the equipment. This device
complies with Part 15 of the FCC Rules. Operation is subject to the following two
conditions: (1) this device may not cause harmful interference, and (2) this device
must accept any interference received, including interference that may cause
undesired operation.
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Electrical power safety notice
Do not position the instrument so that it is difficult to disconnect the power cord.
Equipment misuse notice
If equipment is used in a manner not specified by its manufacturer, protections
against personal injury inherent in the equipment’s design can be rendered
ineffective.
Safety advisories
Consult the "Safety advisories" appendix in this publication for a comprehensive list
of warning advisories and notices.
Operating the system
When operating the system, follow standard quality-control (QC) procedures and the
guidelines presented in this section.
Applicable symbols
Symbol Definition
Manufacturer
Date of manufacture
Authorized representative of the European Community
Confirms that a manufactured product complies with all
applicable European Community directives
or
Australia EMC compliant
Confirms that a manufactured product complies with all
applicable United States and Canadian safety requirements
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Symbol Definition
Consult instructions for use
Alternating current
Electrical and electronic equipment with this symbol may contain
hazardous substances and should not be disposed of as general
waste.
For compliance with the Waste Electrical and Electronic
Equipment Directive (WEEE) 2012/19/EU, contact Waters
Corporation for the correct disposal and recycling instructions.
Serial number
REF
Part number catalog number
Audience and purpose
This guide is intended for personnel who operate and maintain the ACQUITY Arc
system.
Intended use of the ACQUITY Arc system
Waters designed the ACQUITY Arc system to perform liquid chromatography
separations in these environments:
• Pharmaceutical development and discovery
• Quality assurance and quality control
• Chemical materials
• Environmental
• Food safety
The system is not intended for use in diagnostic applications.
Calibrating
To calibrate LC systems, follow acceptable calibration methods using at least five
standards to generate a standard curve. The concentration range for standards must
include the entire range of QC samples, typical specimens, and atypical specimens.
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Quality control
Routinely run three QC samples that represent subnormal, normal, and above-
normal levels of a compound. If sample trays are the same or very similar, vary the
location of the QC samples in the trays. Ensure that QC sample results fall within an
acceptable range, and evaluate precision from day to day and run to run. Data
collected when QC samples are out of range might not be valid. Do not report these
data until you are certain that the instrument performs satisfactorily.
EMC considerations
Canada spectrum management emissions notice
This class A digital product apparatus complies with Canadian ICES-001.
Cet appareil numérique de la classe A est conforme à la norme NMB-001.
ISM classification: ISM group 1 class B
This classification has been assigned in accordance with IEC CISPR 11 Industrial
Scientific and Medical (ISM) instrument requirements.
Group 1 products apply to intentionally generated and/or used conductively coupled
radio-frequency energy that is necessary for the internal functioning of the
equipment.
Class B products are suitable for use in both commercial and residential locations and
can be directly connected to a low voltage, power-supply network.
EC authorized representative
Address Waters Corporation
Stamford Avenue
Altrincham Road
Wilmslow SK9 4AX UK
Telephone [phone]
Fax [phone]
Contact Quality manager
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Table of contents
General information ......................................................................ii
Copyright notice.............................................................................................ii
Trademarks ...................................................................................................ii
Customer comments.......................................................................................ii
Contacting Waters .........................................................................................iii
Safety considerations.....................................................................................iii
Safety hazard symbol notice ..................................................................iii
Power cord replacement hazard..............................................................iv
Hand crush hazard................................................................................iv
High voltage hazard..............................................................................iv
Bottle placement prohibition ..................................................................iv
FCC radiation emissions notice ...............................................................iv
Electrical power safety notice ..................................................................v
Equipment misuse notice........................................................................v
Safety advisories...................................................................................v
Operating the system......................................................................................v
Applicable symbols ................................................................................v
Audience and purpose...........................................................................vi
Intended use of the ACQUITY Arc system ................................................vi
Calibrating...........................................................................................vi
Quality control .................................................................................... vii
EMC considerations....................................................................................... vii
Canada spectrum management emissions notice ..................................... vii
ISM classification: ISM group 1 class B ................................................... vii
EC authorized representative ......................................................................... vii
1 ACQUITY Arc system ...................................................................12
1.1 Features of the ACQUITY Arc system...............................................................12
1.1.1 Flow-through-needle injector ................................................................13
1.1.2 Wash solvent ......................................................................................13
1.1.3 Purge solvent......................................................................................13
1.1.4 Software enhancements ......................................................................13
1.1.5 System components ............................................................................15
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2 Performance optimization ...........................................................20
2.1 General guidelines ........................................................................................20
2.2 Carryover....................................................................................................21
2.3 Reproducibility ............................................................................................21
2.4 Cycle time (between injections)......................................................................21
2.5 Preventing leaks...........................................................................................22
2.5.1 Installation recommendations for fittings ................................................22
2.6 Developing methods .....................................................................................30
2.7 Measuring dwell volume ................................................................................30
2.8 Transferring methods....................................................................................31
2.9 Matching sample diluents ..............................................................................31
3 System preparation .....................................................................32
3.1 Powering-on the system ................................................................................32
3.2 Monitoring module LEDs ................................................................................33
3.2.1 Power LED..........................................................................................33
3.2.2 Status LEDs........................................................................................33
3.3 Monitoring from control panels .......................................................................35
3.3.1 SM FTN-R control panel........................................................................35
3.3.2 QSM-R control panel ............................................................................37
3.3.3 2489 UV-Vis control panel ....................................................................38
3.3.4 2998 PDA control panel ........................................................................39
3.3.5 2424 ELS control panel ........................................................................40
3.3.6 2475 FLR control panel.........................................................................41
3.3.7 2414 RI control panel...........................................................................43
3.4 Opening the console .....................................................................................44
3.4.1 Opening the console from Empower software ..........................................44
3.4.2 Opening the console from MassLynx software..........................................44
3.5 Priming the system.......................................................................................44
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4 System maintenance ...................................................................46
4.1 Contacting Waters Technical Service ..............................................................46
4.1.1 Locating system serial numbers.............................................................46
4.2 Maintenance procedure and frequency.............................................................47
4.3 Spare parts .................................................................................................48
4.4 Troubleshooting with Connections INSIGHT......................................................48
4.4.1 To submit a Connections INSIGHT request:.............................................48
4.5 Configuring maintenance warnings .................................................................49
5 External connections ...................................................................50
5.1 External wiring and plumbing connections........................................................50
5.1.1 External wiring connections ..................................................................51
5.1.2 Plumbing connections ..........................................................................52
5.1.3 Waste-tubing connections.....................................................................53
5.1.4 Connecting to the electricity source........................................................54
5.1.5 Connecting signal cables ......................................................................54
5.2 Ethernet connections ....................................................................................57
A Safety advisories.........................................................................58
A.1 Warning symbols..........................................................................................58
A.1.1 Specific warnings.................................................................................59
A.2 Notices .......................................................................................................60
A.3 Bottles Prohibited symbol ..............................................................................61
A.4 Required protection ......................................................................................61
A.5 Warnings that apply to all Waters instruments and devices ................................61
A.6 Warnings that address the replacing of fuses....................................................65
A.7 Electrical symbols.........................................................................................67
A.8 Handling symbols .........................................................................................68
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B Solvent considerations ................................................................69
B.1 Preventing contamination ..............................................................................69
B.1.1 Clean solvents ....................................................................................69
B.1.2 Solvent quality....................................................................................69
B.1.3 Solvent preparation .............................................................................69
B.1.4 Water ................................................................................................70
B.2 Common solvent properties ...........................................................................70
B.2.1 Solvent miscibility ...............................................................................72
B.2.2 Solvent stabilizers ...............................................................................74
B.2.3 Solvent viscosity .................................................................................74
B.2.4 Wavelength selection ...........................................................................74
C Specifications ..............................................................................80
C.1 System specifications....................................................................................80
C.2 Quaternary Solvent Manager-R specifications ...................................................80
C.3 Sample Manager FTN-R specifications .............................................................82
C.4 30-cm Column heater and 30-cm Column heater and column heater/cooler
specifications ...............................................................................................83
C.5 30-cm Column Heater-Active (CH-30A) specifications........................................84
C.6 Instrument control specifications ....................................................................84
C.7 Environmental specifications ..........................................................................85
C.8 Electrical specifications..................................................................................85
C.9 Physical specifications ...................................................................................85
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1ACQUITY Arc system
The ACQUITY Arc system, which provides true plug-and-play method compatibility
for HPLC and UHPLC separations, can replicate established assays developed on older
HPLC platforms. Using Arc Multi-flow-path technology, the system accepts methods
from any HPLC platform, emulating the dwell volume and mixing behavior of the
original. The system can also improve productivity by pairing with UHPLC column
technology without requiring manual adjustment. Lastly, the ACQUITY Arc system
accepts UPLC methods that have undergone adjustment for larger particle sizes.
1.1 Features of the ACQUITY Arc system
The ACQUITY Arc system performs validated assays using quaternary-based LC
technology that adjusts for the differences between HPLC and UPLC separations.
These are among the system's features and capabilities:
• Pump and sample manager design enhancements that minimize dispersion and
reduce cycle time
• Plug-and-play method compatibility with HPLC or UHPLC
• Direct programing of gradients, in units of pH and ionic strength, using
Auto•Blend Plus technology to vary composition, eliminating the need to manually
prepare mobile phases
• Automatic management of gradient start time and pre-injection steps, in parallel,
minimizing cycle time and maximizing sample throughput
• Automatic counteraction of differences in system dwell volume without the need
to alter gradient-table inputs
• Continuous needle cleansing during the run, minimizing carryover
• Column heater and column heater/cooler options that ensure method
repeatability from lab to lab
• Integrated column-selection valve options that provide fast column changes
• Optical detection options that maximize HPLC and UHPLC performance
• Extend the dynamic range of sample analysis with mass spectrometer options
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1.1.1 Flow-through-needle injector
The sample manager's flow-through-needle mechanism aspirates a sample and holds
it in the sample needle, prepared to inject the sample onto the column. The needle
serves as part of the injection flow path when the sample is pushed onto the column.
The flow-through-needle mechanism facilitates the transfer of HPLC methods,
improves injection accuracy and decreases cycle time for small-volume injections,
and does not require learning new injection modes. Mobile-phase gradients pass
through the needle during injection, ensuring complete sample recovery.
1.1.2 Wash solvent
The wash system uses a single solvent to clean the outside of the sample needle and
prime the wash system. The solvent does not enter the injection flow path.
1.1.3 Purge solvent
The primary function of the purge solvent is to move sample along the injection
pathway. The purge solvent also primes the sample syringe and injection pathway.
The solvent’s injection onto the column occurs only during auto-dilution, when it is
used as the dilution solvent.
1.1.4 Software enhancements
1.1.4.1 Quantum Synchronization
Introducing a low-pressure sample into the high-pressure fluid stream during
injection causes a pressure pulse that can affect chromatographic results. The
Quantum Synchronization feature reduces the affect of this pressure pulse. The
sample manager and solvent manager communicate to automatically coordinate the
injection sequence, enabling the solvent manager to provide additional pressure at
the exact moment the sample manager switches its injector valve to the inject
position, to introduce the low-pressure sample.
1.1.4.2 Gradient Smart Start
Before each sample injection, a sample manager typically performs wash sequences
and aspirates the appropriate sample volume. When these tasks are completed, the
solvent manager begins to deliver the gradient to the injection valve. The dwell
volume of the system affects the amount of time required for this gradient to reach
the column and can be a significant component of the overall cycle time.
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The Gradient Smart Start feature coordinates pre-injection operations and reduces
the effect of the solvent manager’s dwell volume on cycle time. The gradient starts
before or during the sample manager’s pre-injection functions, resulting in significant
time savings.
1.1.4.3 Wash Plungers
Precipitated material that remains on the solvent manager’s pump plungers can
damage the high-pressure seals. The Wash Plungers function washes the seals and
plungers with seal wash solvent, to remove any precipitate. You can use the Wash
Plungers function as needed, or run the function as part of the No-Flow Shutdown
feature.
Tip: The Wash Plungers function is not available when the module is operating.
1.1.4.4 No-Flow Shutdown
The No-Flow Shutdown feature runs the Wash Plungers function after the solvent
manager remains idle for a specified time interval. This feature prevents deposition
of precipitated material on the pump plungers and plunger seals while the system is
idle.
1.1.4.5 Automatic Prime
When you enable the solvent manager's Automatic Prime function, the system
primes the lines of the optional solvent selection valve when a new line is selected.
You can specify the flow rate and duration of the prime for the new solvent line.
Example: If a first injection uses line D1 and a second injection uses line D2, the
solvent manager primes line D2 between the first and second injections.
1.1.4.6 Flow Ramping
Using the Flow Ramping function, you specify the rate at which the solvent manager
increases or decreases flow.
1.1.4.7 Auto Blend Plus
Auto•Blend Plus technology uses pure solvents and concentrated stocks to blend
mobile phase compositions at a specific pH. At the same time, it controls the
concentration of salt or organic solvent, to optimize separations. Use the Auto•Blend
Plus feature to create and store buffer systems in a solvent catalog that all users of
an ACQUITY quaternary solvent manager can share. To prepare and adjust
chromatographic mobile phases, you add acid, base, salt or organic solvent, and
water to the solvent reservoirs. By doing so you can, for example, optimize protein
separations, which are especially sensitive to a buffer's pH and salt concentration.
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You can also optimize reversed-phase separations that are sensitive to pH and
organic-solvent composition.
1.1.5 System components
The following illustration depicts an ACQUITY Arc system stack that includes four
core modules.
Figure 1–1: Instrument modules:
Detector
Column module
Quaternary Solvent Manager-R (QSM-R)
Sample Manager FTN-R (SM FTN-R)
The ACQUITY Arc core system includes these modules:
• Quaternary solvent manager
• Sample manager-flow through needle
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• Column module (30-cm Column Heater, 30-cm Column Heater/Cooler, or 30-cm
Column Heater-Active)
• Detectors (ultra violet/visible, photodiode array, evaporative light scattering,
fluorescence, refractive index, or mass spectrometer)
1.1.5.1 Quaternary solvent manager-R
The quaternary solvent manager-R (QSM-R) is a low-pressure mixing, high-pressure
pump. It provides steady (pulse-free) solvent flow at analytical flow rates to 5
mL/min at 9,500 PSI (655 bar). The quaternary solvent manager can pump four
degassed solvents simultaneously using a gradient proportioning valve (GPV) to
dynamically create a specified composition.
1.1.5.2 Sample manager flow through needle-R
The sample manager flow through needle-R (SM FTN-R) uses a direct-injection
mechanism to inject samples drawn from plates and vials onto a chromatographic
column. Optional extension loops, installed between the sample needle and the
injection valve, can increase the injection volume beyond that of the sample needle.
The sample manager flow through needle can also dilute samples using the auto-
dilution option.
Note: The 50 µL extension loop is included in the standard configuration.
1.1.5.3 30-cm column heater and 30-cm column heater/cooler
The 30-cm Column Heater (30-cm CH) and 30-cm Column Heater/Cooler (30-cm
CHC) are the standard column heating and column heating/cooling modules that
manage and maintain column temperature for ACQUITY Arc systems. Except for the
30-cm CHC module's cooling capability and the additional components required to
support this capability, the modules are identical.
The 30-cm CH module can control compartment temperatures between 20 and 65º
C. Compartment temperature set points range from 5º C above the ambient
temperature to 65º C. The 30-cm CHC module can control compartment
temperatures as high as 65º C. It is capable of heating from 5º C above the ambient
temperature to 65º C, whichever temperature is higher, and of cooling to 15º C
below the ambient temperature or 4º C, whichever temperature is higher.
The base configuration of both modules includes a passive pre-heater that helps
achieve the column's set-point temperature within the column compartment. Both
modules support the column-selection valve option that provides automated column
switching to method conditions. The 30-cm CH and 30-cm CHC column compartment
accommodates LC columns with dimensions up to 7.8-mm internal diameter (ID),
and 30-cm long.
Restriction: If your system configuration includes multiple column compartments,
you can operate the system using only one column compartment at a time.
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Restriction: You cannot use the 30-cm CH or 30-cm CHC module in conjunction
with the optional 30-cm column heater with active pre-heater module (CH-30A).
Restriction: You cannot use the 30-cm CH or 30-cm CHC module's passive pre-
heater in concert with the optional column-selection valve.
1.1.5.4 30-cm column heater-active (optional)
A standard, active pre-heater (APH) assembly heats the incoming solvent before it
enters the column, optimizing thermal performance and minimizing dispersion. The
CH-30A module can control compartment temperatures between 20 °C and 90 °C.
The set points for column temperatures range from 5 °C above the ambient
temperature to 90 °C. The column compartment accommodates LC columns of
dimensions as large as 4.6-mm ID and 30-cm long. A standard, active pre-heater
(APH) assembly heats the incoming solvent before it enters the column.
Restriction: If your system configuration includes multiple column compartments,
you can operate the system using only one column compartment at a time.
1.1.5.5 Detection
The optical detectors compatible with your system detect and quantify
concentrations of sample analyte. The system accommodates these detectors:
• 2998 PDA
• 2489 UV/Vis
• 2414 RI
• 2475 FLR
• 2424 ELS
Note: Although the same model number is utilized, each of these HPLC optical
detectors have specific part numbers to be ordered with the ACQUITY Arc System.
Specific chassis alterations and leak management features have been made
specifically to utilize these detectors on the ACQUITY Arc System. When ordering,
ensure that the detector is specific to, and compatible with, your LC system.
1.1.5.5.1 ACQUITY QDa
The ACQUITY QDa mass detector, which fits atop the system stack, characterizes LC-
separated analytes, determining their mass by means of a photomultiplier detection
system and chromatography data software. The instrument acquires data in one of
these operating modes:
• Scanning, where the instrument scans mass-to-charge (m/z) ratios, to produce a
mass spectrum.
• Selected ion recording (SIR), where the instrument records the signal intensity at
a static m/z ratio.
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• MSe, where the instrument performs both low- and high-fragmentation energy
scans in a single run, to aid in identifying compounds.
• RADAR, where the instrument concurrently performs scanning and SIR
acquisitions.
1.1.5.6 Column technology
The ACQUITY Arc system can accommodate any HPLC or UHPLC column in which the
particle size is 2.5 µm or greater. It is particularly well-suited to column sizes of 3.0
mm ID or greater.
1.1.5.6.1 eCord technology
CORTECS 2.7 µm UHPLC columns include an eCord fob that tracks the usage history
of the column. The eCord fob interacts with the system software, recording
information for as many as 50 sample queues run on the column. In regulated
environments, the eCord fob provides documentation of the column used in the
validation method.
In addition to the variable column usage data, the eCord fob also stores fixed column
manufacturing data, including
• unique column identification;
• certificate of analysis;
• QC test data.
When you attach the eCord fob to the receptacle on the column compartment, the
chip automatically records and stores system information. You need take no further
action.
Restriction: The 30-cm CH and 30-cm CHC modules do not contain eCord fob
functionality. Although eCord fobs can be used with these modules, manufacturing
and usage data go unrecorded.
Restriction: The CORTECS 4.6-mm column does not include eCord functionality.
1.1.5.7 For additional information
On the system documentation CD, you can find this additional information about the
ACQUITY Arc system:
•ACQUITY Arc Quaternary Solvent Manager-R Overview and Maintenance Guide
•ACQUITY Arc Sample Manager Flow Through Needle-R Overview and Maintenance
Guide
•ACQUITY Arc 30-cm Column Heater and 30-cm Column Heater/Cooler Overview
and Maintenance Guide
•ACQUITY Arc 30-cm Column Heater-Active Overview and Maintenance Guide
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•2998 Photodiode Array Detector Overview and Maintenance Guide
•2489 UV Visible Detector Overview and Maintenance Guide
•2414 Refractive Index Detector Overview and Maintenance Guide
•2475 Multi Fluorescence Detector Overview and Maintenance Guide
•2424 Evaporative Light Scattering Detector Overview and Maintenance Guide
•Auto·Blend Plus Technology for Ion Exchange, Size Exclusion, and Reversed-
phase Chromatography Addendum
•ACQUITY QDa Detector Overview and Maintenance Guide
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2Performance optimization
Follow the advice and guidelines in this chapter to help ensure optimum performance
from your system.
2.1 General guidelines
The ACQUITY Arc system provides the ability to replicate established HPLC methods
by emulating a system's dwell volume and mixing behavior. In the instrument
method editor, you can select between Path 1 (HPLC emulation path) and Path 2
(UHPLC path).
You can maximize separation performance by using columns of 3.0 mm ID or larger
that are packed with particles sized 2.5 µm or larger.
Follow these general recommendations when performing a UHPLC analysis:
• Use high quality solvents, buffers, and additives (HPLC or MS-grade).
• Use high quality water (HPLC or MS-grade).
• Always use solvent filters on tubing in solvent bottles.
• Filter buffers with a 0.45-µm filter membrane.
• Keep concentrated stock solutions to use when preparing working solutions, and
refrigerate it when not using it, to maximize shelf life.
• Do not add fresh buffer to old (a practice known as "topping off"); doing so can
promote microbial growth.
• Do not block the degasser's vent line; trim the tubing, if necessary.
• Do not submerge the waste or degasser-vent lines in liquid (refer to the ACQUITY
Arc Quaternary Solvent Manager-R Operator's Overview and Maintenance Guide
for details about how to route the system tubing).
• Keep all solvent lines primed.
• Flush buffers from the system when they are not in use, taking care to avoid
using solvents that can precipitate or otherwise react.
• Do not store the system in 100% acetonitrile; doing so can cause certain
components to fail.
• Use 10-20% organic solvent in water as a storage solvent if you expect the
system to remain idle longer than 24 hours.
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