Planer Kryo360-1.7 Manual

Liquidus Tracker
Operator's Guide

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Liquidus Tracker
© 2011 Planer plc
Operator's Guide
Planer plc reserves the right to alter products and their specifications without notice.
Part Number: MA101894 Issue: 2 Printed: July2011

IContents
Table of Contents
Section I Introduction 2
................................................................................................................................... 21 Symbols
......................................................................................................................................................... 2Symbols used in this manual
......................................................................................................................................................... 3Symbols used on the controller
......................................................................................................................................................... 4Symbols used on the mains adapter
................................................................................................................................... 42 Safety precautions
......................................................................................................................................................... 4First aid ......................................................................................................................................................... 4Safety
................................................................................................................................... 63 EMC precautions
................................................................................................................................... 74 Special terms
................................................................................................................................... 95 Documentation conventions
................................................................................................................................... 96 About the system
......................................................................................................................................................... 10The display and keypad
.................................................................................................................................................. 11Displaying multiple entries
......................................................................................................................................................... 12The connections
......................................................................................................................................................... 14Manual override and reset buttons
......................................................................................................................................................... 15Power backup
Section II Installing the system 18
................................................................................................................................... 181 Installing a Planer pump and dewar
......................................................................................................................................................... 18Connecting the delivery hose
......................................................................................................................................................... 18Filling the dewar
......................................................................................................................................................... 19Fitting the pump
......................................................................................................................................................... 19Testing the dewar
......................................................................................................................................................... 20Removing the pump
................................................................................................................................... 212 Installing a CryoCyl low pressure cylinder
................................................................................................................................... 223 Connecting the controller
................................................................................................................................... 304 Installing the software
Section III Operating the system 32
................................................................................................................................... 321 Switching on the system
......................................................................................................................................................... 32Power-up cycle
................................................................................................................................... 332 Preparing the system
......................................................................................................................................................... 33Setting the clock
......................................................................................................................................................... 34Configuring the Liquidus Tracking parameters
.................................................................................................................................................. 36Liquidus Tracking parameters
........................................................................................................................................... 38Dual solution mode
........................................................................................................................................... 40Single solution mode
........................................................................................................................................... 42Temperature - concentration curve
...................................................................................................................................... 43Target temperature
......................................................................................................................................................... 44Creating a profile
................................................................................................................................... 443 Loading samples

Liquidus TrackerII
......................................................................................................................................................... 45Dual solution carrier
......................................................................................................................................................... 49Condyle carrier
................................................................................................................................... 544 Running a profile
......................................................................................................................................................... 55Running from DeltaT
.................................................................................................................................................. 55PCmode screen
......................................................................................................................................................... 55Running an internal profile
......................................................................................................................................................... 56Profile run screen
.................................................................................................................................................. 56Going to start temperature
.................................................................................................................................................. 57Run screen
......................................................................................................................................................... 57Ending a run
......................................................................................................................................................... 58Alarm signals
................................................................................................................................... 585 Profile warm-up cycle
................................................................................................................................... 596 Retrieving a run
......................................................................................................................................................... 59Retrieving the last run using DeltaT
......................................................................................................................................................... 59Writing the last run to a USBflash drive
Section IV Routine maintenance and
troubleshooting 62
................................................................................................................................... 621 Cleaning the system
................................................................................................................................... 622 Troubleshooting
......................................................................................................................................................... 63No slot option when downloading a profile
......................................................................................................................................................... 63No slot option when retrieving a run
......................................................................................................................................................... 63Controller fails to respond
................................................................................................................................... 633 Returning for service
Section V Additional information 66
................................................................................................................................... 661 Specifications
......................................................................................................................................................... 66System specifications
......................................................................................................................................................... 66Controller specifications
......................................................................................................................................................... 67Chamber specifications
......................................................................................................................................................... 67Fuses ......................................................................................................................................................... 68Batteries ......................................................................................................................................................... 69Alarm output
................................................................................................................................... 692 Decontaminating the system
................................................................................................................................... 703 Safety declaration form
......................................................................................................................................................... 70Safety Declaration
.................................................................................................................................................. 71Declaration of Contamination Status
Index 73

Introduction
Section I

2 Liquidus Tracker
1 Introduction
This guide has been designed to help you get the best from your new system. The guide includes
important information concerning the safe use of the equipment and it is important that you
familiarise yourself with this document before attempting to use the product.
1.1 Symbols
1.1.1 Symbols used in this manual
This shows information or instructions that are related to safety. Failure to follow these
instructions may result in personal or third-party injury.
This symbol is used to introduce important information or instructions related to the use
of the product. Failure to follow these instructions may result in damage to equipment,
samples or data.
The light bulb symbol is used to highlight information and tips that may help you get the
best from the product.

3Introduction
1.1.2 Symbols used on the controller
Refer to these instructions. Failure to follow these instructions may result in personal or
third-party injury
DC power inlet.
15 way D-type connection to the chamber.
Type-B USB connector for linking to a computer running DeltaT.
RS232 connection to the peristaltic pumps.
Connection for USB flash drive.
Ethernet connection.
Alarm output connector.
RST
Reset button. This button will reset the controller. It should only be pressed if the system
fails to respond. See Controller fails to respond.
PRG
Programming button. For service use only.
Manual override buttons. These buttons will heat or cool the chamber and may disrupt the
control of temperature.
63

4 Liquidus Tracker
1.1.3 Symbols used on the mains adapter
The Kryo-360 chamber provides power directly to the controller and for these systems the power
supply is not required.
Refer to these instructions. Failure to follow these instructions may result in personal or
third-party injury
Risk of electric shock
Indoor use only
Direct current (DC)
Alternating current (AC)
1.2 Safety precautions
1.2.1 First aid
IF IN DOUBT, SEEK IMMEDIATE MEDICAL ATTENTION. If any quantity of liquid
nitrogen comes into contact with the skin or eyes, immediately flood that area of the
body with large quantities of unheated water, apply cold compresses and seek medical
attention. If the skin is blistered or there is any possibility that eyes have been affected,
the patient should be taken immediately to a doctor or hospital for treatment.
1.2.2 Safety
Warning
Failure to comply with these instructions could result in serious injury.
Operating the system in a manner not specified within in this manual or under conditions outside
of the specifications may result in the protection offered by the equipment being impaired.
Avoid nitrogen build up. Anywhere liquid nitrogen is used must be well ventilated to reduce the
risk of nitrogen build up. Small volumes of liquid nitrogen convert to very large volumes of gas. This
can result in drowsiness, or in extreme cases, asphyxiation.
When liquid nitrogen is used in confined spaces, oxygen-deficiency alarms must be installed.
Guidance on ventilation requirements are given in BCGA Code of Practice CP30, The safe use of
liquid nitrogen dewars up to 50 litres.
When there is a risk of reduced or insufficient ventilation when a room is unoccupied, then alarms
to indicate oxygen deficiency must be installed and positioned outside of the room so that
operators are aware of the hazard before entering. The operational status of such an alarm should

5Introduction
also be visible from outside the room.
If liquid nitrogen is used in an area that requires forced ventilation, an alarm to indicate its failure
should be fitted.
When dispensing or potentially being exposed to liquid nitrogen, protect the face with a shield and
wear gloves, boots and a protective apron. Prevent spillage into shoes and onto unprotected parts
of the body.
Handle vessels containing liquid nitrogen carefully. Liquid nitrogen boils at -196ºC and both liquid
and gas can cause rapid and severe frostbite. Delicate tissue, e.g. the eyes, can be damaged by
an exposure to the cold gas, which may be too brief to affect the skin of the hands or face.
Stand clear of boiling and splashing liquid nitrogen and its gas when filling a dewar at normal room
temperature or when inserting objects (such as a pump) into the liquid.
Use tongs or wear cryogloves when handling cold or hot objects. Cryogloves are available as an
accessory from Planer plc. When running a program, the chamber and contents may get cold
enough to cause frostbite.
The dewar must be depressurised before the pump can be removed. This is achieved by opening
the pressure-relief valve mounted on the side of the pump, which seals an orifice in the pressure
system by toggle action. Avoid contact with cold nitrogen gas when depressurising the dewar.
The dewars used with this equipment must never be used to store any liquid other than liquid
nitrogen.
Only use vessels designed for working with liquid nitrogen.
Secure the liquid nitrogen container to prevent toppling.
Ensure that the delivery pipe connections are secure and leak-free before supplying liquid nitrogen
to the freezer.
Liquid nitrogen pressure must not exceed 1.7 bar (25 psi).
To maintain protection against electric shock, the mains lead must be properly fitted to a 3-way
mains connector plugged into an earthed mains outlet.
Because of the potentially wet laboratory environment, the operator should be provided with
additional protection against electric shock by supplying mains power to the freezer through a
residual current circuit breaker (RCCB) operating at a differential of 30 mA. Note that care must
be taken to ensure that the freezer is not compromised by faulty equipment sharing the same
supply and causing nuisance trips.
The mains outlets on the chamber’s rear panel are only for use with the Planer LNP4 unit.
The alarm output on the controller must only be connected to equipment that complies with the
requirements in the Alarm output section.
The 15-way socket on the Controller is only to be used for connecting to a Planer chamber.
The RJ45 Ethernet socket is designed for connection to information technology equipment
compliant with IEC 950 or its equivalent. Use with other equipment may compromise the safety of
the device. The unit should only be connected to an Ethernet local area network (LAN) internal to
the building.
The Type-B USB connector on the Controller is only to be used for connecting to the USB port of
a computer that complies with IEC 950 or its equivalent.
69

6 Liquidus Tracker
The Type-A USB connector on the Controller is only to be used for connecting to a USB flash drive
or similar media.
The 9-way D-Type plug on the Controller is only to be used for connecting to the RS232 serial port
of the peristaltic pumps of the liquidus tracking system.
The 6 Way DIN socket is to be used for connection to the liquidus tracker external power supply
if required.
The Sample PRT socket on the chamber's rear panel is only to be used with a Planer Sample
PRT.
User servicing is limited to cleaning and decontamination. All other servicing must only be
undertaken by suitably qualified engineers.
To avoid risk of fire, fuses must always be replaced with the same type and rating.
The Series 500 chambers weigh 23 Kg and require at least two people for safe handling. Do not
attempt to lift using the edges of the case but use the bottom chassis plate.
When switched on but not running a profile, the Series 500 chambers and contents may
eventually become hot enough to cause burns.
When heating under manual control, check the chamber temperature at intervals of 30 seconds or
less to avoid overheating.
Do not move the chamber whilst still wet after a freezing run.
The liquidus tracker requires significant volumes of cryoprotectants, such as Dimethyl Sulfoxide
(DMSO), to be used. These solutions and their vapours are potentially hazardous to health and it
is essential that the material safety data sheets (MSDS) for these materials are examined and
appropriate provisions put in place before use.
The volume of solution used will vary according to the requirements of the profiles to be run.
However, runs will typically use several litres of cryoprotectant.
1.3 EMC precautions
The system fulfils the requirements of EN 61326 Class A equipment.
Note
The following precautions must be taken to ensure that the equipment is not damaged by
electrostatic discharge (ESD), and that its immunity to radio frequency interference is not
compromised.
Ensure that the controller is not running a program and that the mains power is disconnected
before connecting or disconnecting any cable.
Fit the plastic covers and plugs provided to all unused connectors.
Immediately before touching the sample PRT, touch an earthed part, such as the metal surface at
the rear of the controller.
Do not touch any uncovered connectors with your hands or tools, even when the equipment is
switched off.

7Introduction
When connecting the system to a PC via the USB connection, always use a fully screened cable
no longer than 3m.
Take care to avoid placing systems in environments influenced by sources of electromagnetic
interference, such as cyclotrons, large transformers etc.
1.4 Special terms
There are some terms used within this guide that may be unfamiliar to you. These terms are
explained below.
Profile
A profile defines the temperature-time path that the chamber should follow. The profile comprises a
number of steps each of which can either be a hold or a ramp.
Typical profile
Ramp
A ramp is a step in the profile that consists of a rate and a target end temperature.
Hold
A hold is a step in the profile that merely consists of a time. During the hold, the controller will
maintain the chamber at the end of temperature of the previous step until the hold time has elapsed.

8 Liquidus Tracker
Seeding
Tip
Seeding is not appropriate for liquidus tracking. The following description is provided for
completeness but should not be used for a liquidus tracking run.
Within a profile, seeding is a special part of the program designed to trigger the onset of freezing
within the sample being frozen. The Liquidus Tracker supports a type of seeding known as manual
seeding. Manual seeding comprises a seeding temperature and a soak time. When the chamber
reaches the seeding temperature, the controller maintains the temperature for a period defined by
the soak time. This provides time for all the samples to equilibrate at the seeding temperature. The
controller then prompts the user that the samples are ready for seeding. The user would normally
momentarily remove the samples and manually trigger the onset of freezing by touching the straws
with a cold implement; the exact method of seeding is defined by the users' protocols and is outside
the scope of this manual. Once the seeding process has been completed, the user would press a
key on the controller to indicate that seeding was complete and that the freezing profile should
continue as normal. The manual seeding process is summarised in the diagram below.
Profile w ith m an ual s eedin g
Although the controller can support profiles with seeding enabled, this feature is not normally used
with liquidus tracking.

9Introduction
Kryo-file
A kryo-file is a data file containing information about a freezing run including the temperature and
time data for the chamber and sample.
1.5 Documentation conventions
The Liquidus Tracker uses a simple menu system in order to access various features. Some of
these features may require options within menus to be selected. Where more than one selection
needs to be made, this will be shown in the manual as follows.
Top selection > next selection > final selection.
So for example if the manual shows
Main menu > Run from panel > select profile
this would indicate that you should select the Run from panel from the main menu and then select
the appropriate profile.
1.6 About the system
The Liquidus Tracker is a system designed for research into liquidus tracking as a means of
cryopreservation. In the liquidus tracker, the sample to be preserved is provided with a concentration
of cryoprotectant that increases as the temperature of the sample decreases. The product has been
designed to be particularly easy to operate and incorporates a simple, clean user-interface. Profiles
may be stored within the controller's memory but initial creation requires the use of a computer
running DeltaT. Refer to the DeltaT documentation for more information.

10 Liquidus Tracker
1.6.1 The display and keypad
The Liquidus Tracker user interface utilises a very simple display and three button keypad to enable
all features of the system to be accessed.
1. Cool LED. Indicates that the system is applying liquid nitrogen to control the profile.
2. Heat LED. Indicates that the system is applying the heater to control the profile.
3. Information LED. Indicates that the system requires attention.
4. Main display.
5. Run/Enter key. Used to start a run. Also used as an enter key when user input is required.
6. Stop/Right key. Used to stop a run. Also used to scroll through multiple entries when there is
more than one entry that can be selected.
7. Left key. Used to scroll through multiple entries when there is more than one entry that can be
selected.

11Introduction
The two keys, 6 and 7 are also referred to as the cursor keys within this document.
1.6.1.1 Displaying multiple entries
If the message is too long to show on display, the text will be scrolled from right to left. In some
cases there may be multiple selections to display. The main menu is an example where there are
a number of items that need to be presented. When this occurs only one item will be displayed at
any time but the item will be enclosed within <> characters to indicate that there are further entries
to display. Pressing the Left or Right keys will present the additional items.

12 Liquidus Tracker
1.6.2 The connections
The connections to the controller are all made along the underside of the main electronics enclosure.

13Introduction
1. Alarm output
2. Chamber connector
3. Separate power supply compartment. The Kryo-360 chamber provides power directly to the
controller and for these systems the power supply is not required.
4. USB connection for communication to the PC.
5. RS232 connection for driving the peristaltic pumps.
6. DC power inlet.
7. Ethernet output. For remote debugging only.
8. Usb flash drive connector

14 Liquidus Tracker
1.6.3 Manual override and reset buttons
The manual override and reset buttons are located on the side of the controller.
Note
Do not operate the manual override buttons during normal use as they will affect the temperature
control.
1. Manual override: cool.
2. Manual override: heat.
3. PRG: Programming button. This button is for use only by trained service engineers.
4. RST: Reset button. See also Controller fails to respond .
63

15Introduction
1.6.4 Power backup
The controller includes internal power backup, which will allow the system to continue running during
a power failure. This backup can support the controller for up to 1 minute during which time the
control algorithms will continue to function. If power is restored during this period, the controller will
attempt to continue the run. If power is not restored, the controller will terminate the profile and shut
down.