NEW BRUNSWICK SCIENTIFIC Excella E-25 User manual

Excella™ E-25/25R Classic
Incubator Shakers
MANUAL NO: M1353-0050
Revision A
April 24, 2006
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: [phone] •1-800-631-5417
Fax: [phone] •Telex: 4753012 NBSCO
Internet: http://www.nbsc.com •E-mail: [email protected]

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide

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New Brunswick Scientific Co., Inc. User’s Guide
INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
CHINA
New Brunswick Scientific Co., Inc.
A903, 904 Yin Hai Building
No. 250, Cao Xi Road
Shanghai 200235, P.R. China
Tel: [phone] or 5966
Fax: [phone]
E-mail: [email protected]
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
FRANCE
New Brunswick Scientific SARL
12-14, Rond Point des Champs Elysées
75008 Paris
France
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: [phone] or 0800 581331
Fax: [phone]
E-mail: [email protected]
Web: www.nbsuk.co.uk

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
WARNING!
This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage and
personal injury can occur.
Please read entire User’s Guide before attempting to use this unit.

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New Brunswick Scientific Co., Inc. User’s Guide
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
©Copyright 2006 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and
improvement.
Manual Conventions
NOTE:
Notes contain essential information that deserves
special attention.
CAUTION!
Caution messages appear before procedures which, if
caution is not observed, could result in damage to the
equipment.
WARNING!
Warning messages alert you to specific procedures or
practices which, if not followed correctly, could result in
serious personal injury.
Bold Text in boldface type emphasizes key words or
phrases.
This particular Warning message, whether found in the
manual or on the unit, means HOT SURFACE–and
therefore represents a potential danger to touch.
CRUSH WARNING!
Crush Warning messages alert you to specific
procedures or practices regarding heavy objects which,
if not followed correctly, could result in serious personal
injury .

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
Every Instrument manufactured by the
New Brunswick Scientific Co., Inc. is warranted to be free
from defects in material and workmanship. This apparatus, with
the exception of glassware, lamps and electrodes (where supplied),
is warranted against faulty components and assembly for 2 years
in the United States & Canada and for 1 year elsewhere. Our
obligation under this warranty is limited to repairing or replacing
the instrument or part thereof, which shall, upon our examination,
prove to be defective. The warranty period begins at the date of
shipment. This warranty does not extend to any NBS products
which have been subjected to misuse, neglect, accident or
improper installation or application; nor shall it extend to
products which have been repaired or altered outside the
NBS factory without prior authorization from the
New Brunswick Scientific Co., Inc.
W A R R A N T Y

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New Brunswick Scientific Co., Inc. User’s Guide
TABLE OF CONTENTS
1OVERVIEW.........................................................................................................................9
1.1 SPECIFICATIONS............................................................................................................10
1.2 CERTIFICATIONS ...........................................................................................................11
2INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT.......................13
2.1 INSPECTION OF BOXES ..................................................................................................13
2.2 UNPACKING ..................................................................................................................13
2.3 INSPECTION OF EQUIPMENT ..........................................................................................13
2.4 OUT OF BOX CONCERNS ...............................................................................................14
2.5 WARRANTY REGISTRATION ..........................................................................................14
3PREPARING THE LOCATION......................................................................................15
3.1 PHYSICAL LOCATION ....................................................................................................15
3.2 ENVIRONMENT..............................................................................................................15
3.3 ELECTRICAL REQUIREMENTS........................................................................................15
3.4 SPACE REQUIREMENTS .................................................................................................16
4FEATURES........................................................................................................................17
4.1 FRONT PANEL ...............................................................................................................17
5GETTING STARTED.......................................................................................................21
5.1 INSTALLATION OF PLATFORM .......................................................................................21
5.2 INSTALLING FLASK CLAMPS .........................................................................................22
5.3 ELECTRICAL CONNECTIONS..........................................................................................23
6OPERATION .....................................................................................................................25
6.1 STARTING THE SHAKER.................................................................................................25
6.2 CONTINUOUS (UNLIMITED) RUN...................................................................................25
6.3 CHECKING A SETPOINT .................................................................................................26
6.4 TIMED FUNCTIONS........................................................................................................26
6.5 ALARM FUNCTIONS.......................................................................................................27
6.6 TEMPERATURE SETPOINT..............................................................................................27
6.7 TEMPERATURE OFFSET CALIBRATION ..........................................................................28
6.8 POWER FAILURE ...........................................................................................................29
6.9 SPEED CALIBRATION.....................................................................................................29
7PREVENTIVE MAINTENANCE....................................................................................30
7.1 CLEANING EXTERNAL SURFACES..................................................................................30
7.2 FUSE REPLACEMENT.....................................................................................................30
7.3 BELT REPLACEMENT.....................................................................................................33
8TROUBLESHOOTING....................................................................................................35
9REPLACEMENT PARTS & ACCESSORIES...............................................................37

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
9.1 REPLACEMENT PARTS...................................................................................................37
9.2 ACCESSORIES................................................................................................................37
10 DRAWINGS...................................................................................................................39
10.1 LIST OF DRAWINGS.......................................................................................................44
10.2 LIST OF TABLES ............................................................................................................45
11 INDEX.............................................................................................................................46

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New Brunswick Scientific Co., Inc. User’s Guide
1
1 O OV
VE ER RV VI IE EW W
The Excella™ E-25 Classic Incubator and E-25R Classic Refrigerated Incubator Shakers are
large capacity shakers utilizing a UniCentric™ single eccentric counterbalanced drive to provide
horizontal plane rotary motion in a 1-inch (2.54 cm) circular orbit. A Proportional/Integral (PI)
Microprocessor controller with instantaneous digital feedback controls the speed over a range of
50-400 rpm.
The E-25 provides temperature control over a range of 7°C above ambient to 60°C, while the
E-25R provides temperature control over a range of 15°C below ambient (minimum 4°C) to
60°C. Ambient temperature is defined as the temperature within one meter of the shaker.
The shakers may be operated either continuously or in a timed mode via a programmable timer
for shaking periods of 0.1 hr. to 99.9 hrs.
For safe operation, both the E-25 and E-25R are designed with a safety switch that automatically
stops the shaker mechanism when the lid is lifted.
In addition, the Excella E-25 and E-25R are equipped with visual and audible alarms that alert
the user to the following conditions:
• The end of a timed run
• Deviations of shaking speed
• Deviations of temperature setpoint
• Power failure
• Lid open
A wide variety of platforms can be used with the E-25 or E-25R. Dedicated platforms are
available for a variety of flask sizes. Universal platforms, utility trays, utility carriers and test
tube racks are also available.

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
1.1 Specifications
Excella E-25 & E-25R Incubator Shaker
SHAKING
Speed1
Control Accuracy
Indication
Stroke/Orbit
50-400 rpm
±1 rpm
3-digit LED, displayed in 1 rpm increments
1 inch (2.54 cm)
TEMPERATURE
Range (E-25)
Range (E-25R)
Control Accuracy
Indication
7°C above ambient temperature* to 60°C
15°C below ambient temperature* (min. 4°C) to 60°C
±0.1°C @ 37°C
3-digit LED, displayed in 0.1°C increments
Ambient* Operating
Environment 10 - 35°C, 20 to 80% relative humidity, non-condensing
Refrigerant (E-25R only) R-134A
Alarms Visible and audible warning indication when speed deviates
more than 5 rpm, and temperature more than 1°C from
setpoints, and when timer has expired.
Timer 0.1 to 99.9 hours. Shuts off agitation at end of period. Can
be deactivated for continuous operation.
Automatic Restart Automatic restart after power is restored. Setpoints and
operating status are retained in memory during power
interruption.
Drive Interrupt Automatic drive-interrupt when cover is opened.
Electrical Requirements 110/120 VAC, 50/60 Hz
220/240 VAC, 50/60 Hz E-25: 800 VA per shaker E-25R: 1500 VA per shaker
ETL Regulatory
Standards UL 61010A-1 CAN/CSA-C22.2 No. 1010.1
UL 61010A-2-010 CAN/CSA-C22.2 No. 1010.2.010
CE Regulatory Standards See Declaration of Conformity, Section 1.2
Platform 30 X 18 inches (76 X 46 cm)
Overall Dimensions 50 inches W X 30.5 D X 41.7 H (127 X 77 X 85 cm)
Chamber Dimensions 33.5 inches W X 23 D X 19 clearance above platform
(85 X 58 X 48 cm)
Net Weight E-25: 420 lbs ( 191 kg), E-25R: 450 lbs ( 204.5 kg)
Gross Weight E-25: 450 lbs ( 204.5 kg), E-25R: 480 lbs (218 kg)
1See NOTE below.
* Ambient temperature is defined as the temperature measured one meter from the
shaker.
NOTE:
Use of baffled flasks will significantly reduce maximum speed for any
shaker. We may be unable to improve this maximum speed by using an
alternative motor pulley. Contact your NBS representative for more
information.

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New Brunswick Scientific Co., Inc. User’s Guide
1.2 Certifications
The Excella E-25 & E-25R have been tested to ETL standards, to comply with UL and
CAN/CSA electrical safety standards (see “ETL Regulatory Standards” in the
specifications table, Section 1.1).
As attested in the CE Declaration of Conformity reproduced on the following page, they
also conform to the appropriate CE standards (see also “CE Regulatory Standards” in the
specifications table).

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide

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New Brunswick Scientific Co., Inc. User’s Guide
2
2 I IN
NS SP PE EC CT TI IO ON N,
, V VE
ER RI IF FI IC CA AT TI IO ON N &
&
U
UN
NP PA AC CK KI IN NG G O OF F E
EQ
QU UI IP PM ME EN NT T
2.1 Inspection of Boxes
After you receive your order from New Brunswick Scientific, inspect the boxes carefully
for any damage that may have occurred during shipping. Report any damage to the
carrier and to your local NBS Sales Order Department.
2.2 Unpacking
Save all packing materials and User’s Guide. If any part of your order was damaged
during shipping, missing pieces, or fails to operate properly, please fill out the Customer
Satisfaction Form 6300 provided and return by fax.
WARNING!
Do not attempt to lift the E-25 or E-25R by hand. Always use a lifter or
suitable equipment when raising the unit.
There are two small plastic straps holding the bearing housing in place during shipping.
Remove the straps from the bearing housing once the unit is unpacked and inspected.
2.3 Inspection of Equipment
Verify against your NBS packing list that you have received the correct materials.
You should have received a Power Kit (power cord, fuse, hex wrench and key) with your
shaker. Make sure your electrical supply matches the electrical specification of the
power kit and the shaker (both of which should be the same).
NOTE:
Use of the Excella E-25/25R Shakers requires a platform, which is a separate
item. See the Available Platforms list in Section 9.2.

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
2.4 Out of Box Concerns
If any part of your order was damaged during shipping, is missing pieces, or fails to
operate properly, please contact your sales representative and also fill out Customer
Satisfaction Form 6300, included with your equipment, and return it by fax.
2.5 Warranty Registration
Please complete and return your warranty card or register electronically at our Website:
www.nbsc.com

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New Brunswick Scientific Co., Inc. User’s Guide
3
3 P PR
RE EP PA AR RI IN NG G T TH HE E L
LO
OC CA AT TI IO ON N
3.1 Physical Location
It is essential that the instrument be situated in a area where there is sufficient space for
the shaker and platform to clear walls and obstructions during operation. The surface on
which the unit is placed must be smooth, level, and able to support the shaker under full
load operating conditions.
WARNING!
Do not attempt to lift the E-25 or E-25R by hand. Always use a lifter or
suitable equipment when raising the unit.
The feet can be adjusted for necessary leveling. Loosen the locking nuts on the threaded
studs attached to the feet of the shaker. Retighten when you have achieved the correct
level for your shaker.
3.2 Environment
The shaker is designed to operate optimally in the following ambient conditions:
• 10 - 35°C
• 20 to 80% Relative Humidity non-condensing
3.3 Electrical Requirements
The E-25 shakers can be equipped to run on:
 100 Volts, 50/60 Hz, 800 VA maximum
 120 Volts, 60 Hz, 800 VA maximum
 230 Volts, 50 Hz, 800 VA maximum
The E-25R shakers can be equipped to run on:
 100 Volts, 50/60 Hz, 1500 VA maximum
 120 Volts, 60 Hz, 1500 VA maximum
 230 Volts, 50 Hz, 1500 VA maximum
In all cases, voltage variation must not exceed ±10%.

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
3.4 Space Requirements
Figure 1: Space Requirements
Height with
lid open:
65¼ inches
(165.7 cm)
50 inches
(127 cm)
24 inches
(61 cm) for access to
service door
34½ inches
(87.6 cm) front to back
clearance
4 inches
(10 cm) front
& left side
clearance

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New Brunswick Scientific Co., Inc. User’s Guide
4
4 F FE
EA AT TU UR RE ES S
4.1 Front Panel
The keypad on the top (front) panel is the primary user interface to command the Excella
E-10. This section will acquaint you with the keypad’s user interface keys, LED display,
status indicators and function indicators.
Figure 2: Front Panel
Figure 2a: Keypad
USER
INTERFACE
KEYS
LED
DISPLAY
STATUS
INDICATORS
FUNCTION
INDICATORS
KEYPAD
ON/OFF SWITCH

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
4.1.1 LED Display
The digital display on the control panel is a three-digit LED DISPLAY. During
normal shaker operation, the display will indicate:
• Shaker status (On/Off)
• Shaking speed
• Chamber temperature
• Setpoints
• Hours remaining (in a timed run)
• Lid open (“LID”)
4.1.2 User Interface Keys
As you can see in Figure 2a, the keypad has four user interface keys: START/STOP, S,
Tand SELECT. This is how they are used:
• START/STOP This key is used to start or stop the shaker. It will
also activate or stop the timer when a timed run is
desired.
• V(UP), W(DOWN) These keys are used to adjust the setpoint of a
displayed parameter up or down. They also allow
the user to enter the SET MODE for setpoint
changes.
• SELECT This key is used to change the displayed parameter.
4.1.3 Status Indicators
Four status indicator lights are located to the left of the LED DISPLAY. They are:
• SET Indicates that the shaker is in the SET MODE,
when setpoints are being displayed and can be
altered. This is activated by the SELECT key
or by pressing the V(UP) or W(DOWN) arrow.
• POWER Illuminates and blinks during power up or if
power is interrupted during a run. Press the
SELECT key and change to another function to
turn off this indicator.

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New Brunswick Scientific Co., Inc. User’s Guide
• HEAT Illuminates to indicate that the heater is on.
• TIME Indicates that the timer is in operation. The
shaker can be programmed to run for a preset
time from 0.1 to 99.9 hours. The timer can be
disengaged without stopping an ongoing run.
4.1.4 Function Indicators
Four function indicator lights are located to the right of the LED DISPLAY. They
indicate the current parameter(s) being displayed:
• °C Interior chamber temperature. Can be set from
4°C to 60°C, when in SET MODE, using the
V(UP) or W(DOWN) arrow key. It indexes at
0.1°C increments unless the key is pressed for
4 seconds, after which it indexes more rapidly.
• RPM Revolutions per minute. When in SET MODE,
use the V(UP) or W(DOWN) arrow key to
change the speed. It indexes at 1 RPM
increments unless the key is pressed for 4
seconds, after which it indexes more rapidly.
• HRS Time remaining in a timed run. Can be set
from 0.1 to 99.9 hours, in 0.1 increments or, if
the V(UP) or W(DOWN) arrow key is pressed
for 4 seconds, after which it indexes more
rapidly.
The countdown begins when the START/STOP
key is pressed. If the START/STOP key is
pressed again, the shaking stops (but
temperature is maintained) and the timer
pauses until the START/STOP key is pressed
again.
When a timed run ends, the HRS indicator will
blink. Press the SELECT key and change to
another function to turn off this indicator.

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M1353-0050 YExcella™ E-25/E-25R Classic Incubator Shakers User’s Guide
• MUTE This feature is activated by the SELECT key.
When activated, the audible alarm is muted,
and remains so until is is reactivated with the
SELECT key. If MUTE is activated when the
shaker is turned off using the ON/OFF switch, it
will remain engaged when the machine is
powered up again. To activate (or deactivate)
the MUTE function, press the SELECT key until
the MUTE indicator illuminates (or goes out),
then press START/STOP key.