PolyScience Histology Bath User manual

Operator’s Manual
Histology Bath
110-827
09.12.11

110-827 1
Table of Contents
Introduction............................................................................................................................................. 2
General Safety Information................................................................................................................................ 2
Safety Recommendations................................................................................................................................... 3
Regulatory Compliance & Testing..................................................................................................................... 3
Unpacking Your Histology Bath ........................................................................................................................ 4
Contents ................................................................................................................................................................. 4
Components & Controls ......................................................................................................................... 5
Installation .............................................................................................................................................. 6
General Site Requirements ................................................................................................................................ 6
Adding Liquid to the Reservoir.......................................................................................................................... 6
Electrical Power.................................................................................................................................................... 6
Normal Operation ....................................................................................................................................7
Routine Maintenance & Troubleshooting ............................................................................................ 8
Draining the Reservoir ........................................................................................................................................ 8
Cleaning the Bath .................................................................................................................................................8
Air Vents................................................................................................................................................................. 8
Air Filter ................................................................................................................................................................. 8
Troubleshooting Chart......................................................................................................................................... 9
Technical Information ............................................................................................................................ 9
Performance Specifications ............................................................................................................................... 9
Replacement Parts................................................................................................................................ 10
Equipment Disposal (WEEE Directive) ................................................................................................ 10
Service and Technical Support ............................................................................................................ 10
Warranty.................................................................................................................................................. 11

110-827 2
Introduction
Thank you for choosing this Histology Bath. Its low working temperature (-60°C / -76°F) freezes
samples quickly, preventing microscopic ice crystals from developing and samples from distorting. It
is ideal for fast-freezing tissues for enzyme studies. The Histology Bath is not a controlled cooling
source; it is designed to run at maximum cooling.
Standard features include:
• Continuous cooling to -60°C / -76°F
• 1.9 liter stainless steel reservoir and reservoir cover
• Immersion basket and chuck holder
• Front-mounted reservoir drain
• DuraTop™ insulating top deck
General Safety Information
When installed, operated, and maintained according to the directions in this manual and common
safety procedures, your Histology Bath should provide safe operation. Please ensure that all
individuals involved in the installation, operation, or maintenance of this Histology Bath read this
manual thoroughly prior to working with the unit.
This symbol marks chapters and sections of this instruction manual that are particularly
relevant to safety. When attached to the unit, this symbol draws attention to the relevant
section of the instruction manual.
This symbol indicates that hazardous voltages may be present.
This symbol marks information that is particularly important.
This symbol indicates alternating current.
This symbol on the Power Switch / Circuit Breaker indicates that it places the unit into a fully
powered state.
This symbol on the Power Switch / Circuit Breaker indicates that it disconnects power to the
unit.
This symbol indicates a protective conductor terminal.
Read all instructions pertaining to safety, set-up, and operation.
Proper operation is the user’s responsibility.

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Safety Recommendations
To prevent injury to personnel and/or damage to property, always follow your workplace’s safety
procedures when operating this equipment. You should also comply with the following safety
recommendations:
WARNING: The Histology Bath uses isopentane as the bath fluid. Due to the flammability of
this liquid, always use the Histology Bath under a fume hood.
WARNING:
• Always connect the power cord on this Histology Bath to a grounded (3-prong) power outlet.
Make certain that the outlet is the same voltage and frequency as your unit.
• Never operate the Histology Bath with a damaged power cord.
• Always turn the Histology Bath Off and disconnect mains power before performing any
maintenance or service.
WARNING:
• Never operate the Histology Bath without bath fluid in the reservoir. Periodically check the
reservoir to ensure that the liquid depth is within acceptable levels. Always refill the
reservoir using the same bath fluid type that is already in the reservoir.
• Use compatible bath fluids only.
• Always drain all fluid from the reservoir before moving or lifting your Histology Bath. Be
sure to follow your organization’s procedures and practices regarding the safe lifting and
relocation of heavy objects.
WARNING: Always allow the bath fluid to return to ambient temperature before draining.
WARNING: It is the user’s responsibility to properly decontaminate the unit in the event
hazardous materials are spilled on exterior or interior surfaces. Consult manufacturer if there
is any doubt regarding the compatibility of decontamination or cleaning agents.
Regulatory Compliance & Testing
CE
EC Low Voltage Directive 2006/95/EC
EC Electromagnetic Compatibility Directive 2004/108/EC
IEC 61010-1-2001
IEC 61010-2-2001
IEC 61326:2005 / EN 61326 : 2006

110-827 4
Unpacking Your Histology Bath
Your Histology Bath is shipped in a special carton. Retain the carton and all packing materials until
the unit is completely assembled and working properly. Set up and run the unit immediately to
confirm proper operation. Beyond one week, your unit may be warranty repaired, but not replaced.
If the unit is damaged or does not operate properly, contact the transportation company, file a
damage claim, then contact the company where your unit was purchased.
WARNING: Keep unit upright when moving. Be sure to follow your company’s procedures and
practices regarding the safe lifting and relocation of heavy objects.
Contents
The items included with your Histology Bath are:
• Histology Bath
• Reservoir Cover
• Immersion Basket and Chuck Holder
• Power Cord
• Operator’s Manual

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Components & Controls
Power Switch / Circuit Breaker
Reservoir Cover
Drain Valve
Drain Port
IEC Power
Connection
Reusable Air Filter
(behind grille)

110-827 6
Installation
Your Histology Bath is simple to setup and install. The only tool required is a container for adding a
suitable fluid to the bath reservoir.
General Site Requirements
WARNING: The Histology Bath uses isopentane as the bath fluid. Due to the flammability of
this liquid, always use the Histology Bath under a fume hood.
Locate your Histology Bath on a level surface in an area that is free from drafts and wide ambient
temperature variations, such as near heater or air conditioning vents. Do not place it where there
are corrosive fumes, excessive moisture, or in excessively dusty areas.
Position the unit to allow for unobstructed air flow through the side and rear vents. A space of at
least 4 inches (10.2 cm) should be provided behind the unit.
Avoid voltage drops by using properly grounded power outlets wired with 14 gauge or larger
diameter wire and if possible, be close to the power distribution panel. The use of extension cords is
not recommended; this will reduce the potential for problems caused by low line voltage.
Adding Liquid to the Bath Reservoir
WARNING: Fill reservoir with fluid before applying power to the unit.
WARNING: Read the safety data sheet for the bath fluid being used carefully before filling
reservoir.
The Histology Bath is designed for use with isopentane (also known as 2-methlybutane).
Fill bath so that the liquid level is approximately 1 inch (2.54 cm) from the top for best refrigeration
performance. Be sure to allow for the fluid displacement that will result when the immersion basket
and samples are placed in the reservoir.
WARNING: Always drain all fluid from the reservoir before moving or lifting your Histology
Bath. Be sure to follow your organization’s procedures and practices regarding the safe lifting
and relocation of heavy objects.
WARNING: To avoid the potential for burns, allow the Histology Bath to return to ambient
temperature before cleaning or performing any maintenance.
Electrical Power
WARNING: The Histology Bath’s power cord must be connected to a properly grounded
electrical receptacle. Make certain that this electrical outlet is the same voltage and frequency
as your unit. The correct voltage and frequency for your Histology Bath are indicated on the
identification label on the rear of the unit.
CAUTION: The use of an extension cord is not recommended. If one is necessary, it must be
properly grounded and capable of handling the total wattage of the unit. The extension cord
must not cause more than a 10% drop in voltage to the unit.
Plug the Histology Bath’s power cord into a properly grounded electrical outlet of the correct
voltage and frequency.

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Normal Operation
WARNING: Due to the flammable nature of isopentane, the Histology Bath should always be
used under a fume hood. Be sure to wear appropriate protective equipment, such as splash
goggles and gloves.
NOTE: The Histology Bath operates at maximum cooling performance.
Place the Power Switch / Circuit Breaker on the front of the Histology Bath in the On position. The
compressor will start up and the unit will begin cooling. It will take about one hour for the unit to
achieve a -60°C / -76°F working temperature.
Once the unit has reached working temperature, remove the reservoir cover and slowly submerge
the immersion basket in the reservoir. Use the tissue mounts (not supplied) and chuck holder to
position the samples properly in the sample basket. Replace the reservoir cover.
Immersion Basket
Tissue Mounts
(user-supplied)
Chuck Holder

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Routine Maintenance & Troubleshooting
WARNING: Hazardous voltages may be present. Disconnect power before performing
maintenance.
Draining the Reservoir
To drain fluid from the bath, attach a short length of suitable 11.5 mm ID / 0.45 inch ID tubing to the
drain port and secure it using a hose clamp with a minimum ID of 18 mm / 0.7 inch. Open the drain
valve using a flat blade screwdriver. When closing the valve, do not over tighten.
WARNING: Be sure to close the drain valve before refilling the bath reservoir. Do not over
tighten.
Cleaning the Bath
Thoroughly clean the bath before each use. Use only mild soap and water when cleaning. Do not use
steel wool as damage to the unit may result. Non-steel scouring pads are acceptable.
The entire unit is housed in a tough, well-insulated stainless steel casing that is corrosion and
chemical resistant.
Air Vents
The unit draws air through the rear and exhausts it through the side vents. The vents should be
inspected regularly for the accumulation of dust and cleaned as required.
Air Filter
To keep the refrigeration system operating at optimum cooling capacity, the reusable filter located
at the rear of the unit should be kept free of dust and dirt. It should be checked on a scheduled basis
and cleaned as required.
To access the air filter, remove the three screws securing the grille cover to the unit’s housing,
detach the grille, and then gently remove the air filter from the filter compartment. Use a mild
detergent and water solution to wash off any accumulated dust and dirt and then rinse and dry
thoroughly before reinstalling.
Grille
Air Filter

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Troubleshooting Chart
Problem Cause Corrective Action
No power to unit Electrical power
disconnected
Power Switch / Circuit
Breaker in Off position
Compressor overload switch
has tripped
Check that electrical cord is secure and plugged
into an operating electrical outlet.
Place Power Switch / Circuit Breaker in On
position.
Allow approximately 10 minutes for unit to restart.
No or insufficient
cooling
Blocked air flow
High ambient temperature
Improper line voltage and/or
frequency
Check air vents for blockage.
Check that air flow around unit is not restricted
and that room temperature does not exceed 95°F
/ 35°C.
Verify that Mains voltage during start cycle is
within 10% of rated voltage.
Gradual loss of cooling Blocked air flow Check air vents for blockage.
Compressor shakes,
stalls, or continually
restarts
Compressor cycling or
overload
Check air vents for blockage.
Check that air flow around unit is not restricted
and that room temperature does not exceed 95°F
/ 35°C.
Verify that Mains voltage during start cycle is
within 10% of rated voltage.
Check compressor fan for operation (listen for fan
noise, check airflow through unit).
Technical Information
Performance Specifications
Working Temperature: Fixed at -60°C / -76°F
Reservoir Capacity: 1.9 liters
Tank: Insulated stainless steel
Bath Work Area: 4.8 ∅x 6.6 inches deep / 12.2 ∅x 16.8 cm deep
Overall Dimensions (L x W x H): 22.75 x 11 x 11 inches / 57.8 x 27.9 x 27.9 cm
Electrical Requirements: 60 Hz: 120V, 60Hz, 5.0A
50 Hz: 240V, 50Hz, 2.5A
Specifications subject to change without notice.
Environmental Conditions Indoor use only
Maximum Altitude: 2000 meter
Operating Ambient: 5° to 35°C (41° to 95°F)
Relative Humidity: 80%, non-condensing
Installation Category: II
Pollution Degree: 2

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Replacement Parts
Description Part Number
Immersion Basket 703-023
Holder 703-067
Air Filter 305-054
Operator’s Manual 110-827
Equipment Disposal (WEEE Directive)
or
This equipment is marked with the crossed out wheeled bin symbol to indicate it is covered by the
Waste Electrical and Electronic Equipment (WEEE) Directive and is not to be disposed of as unsorted
municipal waste. Any products marked with this symbol must be collected separately, according
to the regulatory guidelines in your area.
It is your responsibility to correctly dispose of this equipment at lifecycle-end by handing it over to
an authorized facility for separate collection and recycling. It is also your responsibility to
decontaminate the equipment in case of biological, chemical and/or radiological contamination, so
as to protect the persons involved in the disposal and recycling of the equipment from health
hazards. By doing so, you will help to conserve natural and environmental resources and you will
ensure that your equipment is recycled in a manner that protects human health.
Requirements for waste collection, reuse, recycling, and recovery programs vary by regulatory
authority at your location. Contact your local responsible body (e.g., your laboratory manager) or
authorized representative for information regarding applicable disposal regulations.
Service and Technical Support
If you have followed the troubleshooting steps outlined previously and your Histology Bath still fails
to operate properly, contact the supplier from whom the unit was purchased. Have the following
information available for the customer service person:
• Model, Serial Number, and Voltage (from back panel label)
• Date of purchase and purchase order number
• Supplier’s order number or invoice number
• A summary of the problem

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Warranty
The manufacturer agrees to correct for the original user of the product, either by repair (using new
or refurbished parts), or at the manufacturer’s election, by replacement (with a new or refurbished
product), any defects in material or workmanship which develop during the warranty period. The
standard warranty is twenty-four (24) months after delivery of the product. In the event of
replacement, the replacement unit will be warranted for the remainder of the original warranty
period or ninety (90) days, whichever is longer. For purposes of this limited warranty, “refurbished”
means a product or part that has been returned to its original specifications. In the event of a
defect, these are your exclusive remedies.
If the product should require service, contact the manufacturer’s/supplier’s office for instructions.
When return of the product is necessary, a return authorization number is assigned and the product
should be shipped, transportation charges pre-paid, in either its original packaging or packaging
affording an equal degree of protection to the indicated service center. To insure prompt handling,
the return authorization number must be placed on the outside of the package. A detailed
explanation of the defect should be enclosed with the item.
The warranty shall not apply if the defect or malfunction was caused by accident, neglect,
unreasonable use, improper service, acts of God, modification by any party other than the
manufacturer, or other causes not arising out of defects in material or workmanship.
EXCLUSION OF IMPLIED WARRANTIES. THERE ARE NO WARRANTIES, EXPRESSED OR IMPLIED,
INCLUDING, BUT NOT LIMITED TO, THOSE OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR
PURPOSE WHICH EXTEND BEYOND THE DESCRIPTION AND PERIOD AS STATED IN THE
OPERATOR’S MANUAL INCLUDED WITH EACH PRODUCT.
LIMITATION ON DAMAGES. THE MANUFACTURER’S SOLE OBLIGATION UNDER THE WARRANTY
IS LIMITED TO THE REPAIR OR REPLACEMENT OF A DEFECTIVE PRODUCT AND THE
MANUFACTURER SHALL NOT, IN ANY EVENT, BE LIABLE FOR ANY INCIDENTAL OR
CONSEQUENTIAL DAMAGES OF ANY KIND RESULTING FROM USE OR POSSESSION OF THIS
PRODUCT.
Some states do not allow: (A) limitations on how long an implied warranty lasts; or (B) the exclusion
or limitation of incidental or consequential damages, so the above limitations or exclusions may not
apply to you. This warranty gives you specific legal rights and you may have other rights that vary
from state to state.
Manufactured by:
PolyScience
6600 W. Touhy Avenue Niles, IL 60714 U.S.A.
1-800-229-7569 1-847-647-0611
www.polyscience.com