Promega GloMax 20/20 User manual

Revised 5/21
TM276
TECHNICAL MANUAL
GloMax®20/20 Luminometer
Instrucons for Use of Products
E5311, E5351 and E5361

All technical literature is available at: www.promega.com/protocols/
Visit the web site to verify that you are using the most current version of this Technical Manual.
E-mail Promega Technical Services if you have questions on use of this system: [email protected]
GloMax®20/20 Luminometer
Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
www.promega.com TM276 · Revised 5/21
1. Description.........................................................................................................................................3
1.A. Inspection..................................................................................................................................3
1.B. Precautions ...............................................................................................................................3
2. Product Components...........................................................................................................................4
3. Hardware Overview ............................................................................................................................4
4. Setting up the GloMax®20/20 Luminometer......................................................................................... 5
4.A. Setup.........................................................................................................................................5
4.B. Software Installation and System Connection ...............................................................................5
5. Operating the GloMax®20/20 Luminometer ........................................................................................ 6
5.A. Touch Screen Basics.................................................................................................................... 6
5.B. Home Screen .............................................................................................................................6
5.C. Navigation ................................................................................................................................. 6
5.D. Running a Promega Protocol .......................................................................................................7
5.E. Creating a New Protocol..............................................................................................................7
5.F. Kinetic Measurements ................................................................................................................ 8
5.G. Default Protocol .........................................................................................................................8
5.H. Running a User Protocol .............................................................................................................8
6. Measuring Your Samples .....................................................................................................................8
7. Saving Your Protocol ........................................................................................................................... 9
8. Tools................................................................................................................................................ 10
8.A. Settings ................................................................................................................................... 10
8.B. Diagnostics ............................................................................................................................. 10
8.C. Using the Light Standard........................................................................................................... 11
8.D. Removing the Light Standard .................................................................................................... 11
9. GloMax®20/20 Luminometer Fluorescent Module.............................................................................. 12
9.A. Installing the Fluorescent Module.............................................................................................. 12
9.B. Touch Screen Controls .............................................................................................................. 13
9.C. Removing the Fluorescent Module ............................................................................................. 13

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TM276 · Revised 5/21 www.promega.com
10. Troubleshooting................................................................................................................................ 14
11. Appendix.......................................................................................................................................... 15
11.A. General Care............................................................................................................................ 15
11.B. Warranty and Service ............................................................................................................... 15
11.C. Specifications........................................................................................................................... 16
11.D. Certificate of Decontamination.................................................................................................. 17
11.E. Related Products ...................................................................................................................... 18
11.F. Summary of Changes................................................................................................................. 18

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
www.promega.com TM276 · Revised 5/21
1. Description
The GloMax®20/20 Luminometer is an ultrasensitive, compact laboratory instrument for measuring luminescent
materials. Specifically, the GloMax®20/20 Luminometer accommodates measurement of firefly and Renilla luciferase for
genetic reporter studies and ATP-based biomass analysis, and so is an ideal platform for reading Promega bioluminescent
assays. Additionally, the GloMax®20/20 Luminometer will measure chemiluminescence from both horseradish
peroxidase and alkaline phosphatase. The touch screen on the luminometer allows easy setup and operation. The
GloMax®20/20 Luminometer also features optional modules for measuring certain fluorescent materials including
fluorescein, Hoescht dye, 4-MU, PicoGreen®dye and RiboGreen®dye.
1.A. Inspection
When you receive your luminometer, please inspect the package carefully to make sure all accessories are present.
5418TA
5431TA
Power Supply Kit
Tube Holder
Luminometer
USB Cable
Figure 1. Standard accessories supplied with the GloMax®20/20 Luminometer (all models).
1.B. Precautions
The GloMax®20/20 Luminometer is intended for indoor use only. Wipe up spills immediately. The GloMax®20/20
Luminometer contains sensitive optical components and precision-aligned mechanical assemblies. Avoid rough
handling.
13514TA

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TM276 · Revised 5/21 www.promega.com
2. Product Components
PRODUCT SIZE CAT.#
GloMax® 20/20 Luminometer 1 each E5311
Includes:
•1 Luminometer
• 1 1.5ml Microcentrifuge Tube Holder
• 1 USB Cable
• 1 Power Supply Kit
• 1 Quick Start Guide (FB081)
Items Available Separately
PRODUCT SIZE CAT.#
GloMax® 20/20 Fluorescent Module, UV 1 each E5351
GloMax® 20/20 Fluorescent Module, Blue 1 each E5361
Tripp Lite USB to Serial Adapter 1 each TS2520
3. Hardware Overview
17690TA
On/Off switch
Power connector
USB port
Serial port
Figure 2. Rear view of the GloMax®20/20 Luminometer.

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
www.promega.com TM276 · Revised 5/21
4. Setting up the GloMax®20/20 Luminometer
4.A. Setup
1. Place the GloMax®20/20 Luminometer on a flat, level surface. Allow at least 6 inches (16cm) of vertical clearance
to open and close the lid. Position the luminometer so that the touch screen faces you.
2. Connect the power supply into the back of the luminometer.
3. Plug the power cord into a wall outlet. See Section 11.C for power requirements.
4. Turn the On/Off switch to the “On” position (Figure 2).
Note: Touch screen calibration can drift during shipment. If this happens, touch screen alignment will be affected
and buttons may be difficult to press or unresponsive. See Section 8.B for Touch Screen Calibration.
4.B. Software Installation and System Connection
To export data from the luminometer to an Excel spreadsheet, you must first install the GloMax®Spreadsheet Interface
Software on a PC with an available serial port or USB connector. The GloMax®Spreadsheet Interface Software is
compatible with 32-and 64-bit PCs using Windows®7 Professional Service Pack 1 with either Microsoft®Office 2010
Home and Business Edition (32- and 64-bit versions) or Microsoft®Office 2007 (32-bit version).
1. Download the software from the Promega website: www.promega.com/resources/software-firmware/
glomax-systems/glomax-20-20-luminometer/. This will initiate the installation program. When
installation is complete, the GloMax®Spreadsheet Interface Software icon will appear on your PC desktop and in
the Programs menu.
2. To establish a connection between the luminometer and the PC, connect the USB cable between the luminometer
and your PC. If connecting to the PC with a USB/RS-232 adaptor, we recommend using the Tripp-Light, Model#
U209-000-R, which can be purchased from Tripp Lite (Cat.# U209-000-R), or from Promega (Cat.# TS2520).
When using a USB-Serial adaptor, you may need to download the appropriate computer driver available at:
www.tripplite.com. Select the PC COM port in the software using the Select COM port button to establish a
connection with the instrument.
Alternatively, connect the luminometer to a printer to print the data as they appear onscreen. A thermal printer
(Cat.# E2821) is available separately from Promega.

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TM276 · Revised 5/21 www.promega.com
5. Operating the GloMax®20/20 Luminometer
5.A. Touch Screen Basics
The touch screen monitor on the GloMax®20/20 Luminometer is sensitive to the light pressure of a fingertip. After
turning ON the luminometer, the touch screen will automatically light up with the Home Screen. After 15 minutes
without activity or user stimulation, the touch screen will hibernate to conserve power. To reactivate, lightly touch the
screen once. To select a function, touch the button corresponding to the function once.
5.B. Home Screen
The Home Screen (Figure 3) provides orientation for the multiple functions of the GloMax®20/20 Luminometer.
From the Home Screen, you can select Protocols, Tools, or Help. You can also measure luminescent samples from
the Home Screen by selecting Measure Luminescence.
Figure 3. Home Screen of the the GloMax®20/20 Luminometer.
5.C. Navigation
A navigation bar at the bottom of the touch screen allows you to access menus quickly. Selecting the Home key takes
you to the Home Screen. The Help menu is also available from the navigation bar. You can select Tools to access the
‘Tools and Settings’ options. Select Protocols to access Promega protocols, create a new protocol, access a saved
protocol or use the default protocol.
5383TA

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5.D. Running a Promega Protocol
The GloMax®20/20 Luminometer accommodates the measurement requirements for luminescent assays from
Promega. Touch Run Promega Protocol from the Protocol Menu (Figure 4), and select the protocol that corresponds
to your assay system. For example, if you are using the Steady-Glo®Assay for a luciferase gene reporter study, select
Steady-Glo. The luminometer will open a Steady-Glo®protocol with the recommended measurement settings
automatically programmed. Touch OK to accept the parameters and return to the Home Screen. You are now ready to
measure your samples.
5389TA
Figure 4. The Protocol Menu.
5.E. Creating a New Protocol
If you touch Create New Protocol, the GloMax®20/20 Luminometer prompts you to adjust the integration time
(Figure 5). The integration time is another term for the measurement duration. Touch Integration, then use the up
and down arrow keys to increase or decrease the integration time. When you are finished, touch Save As to save your
protocol under a specific name of your choice. Alternatively, touch OK to return to the Home Screen to measure your
samples. The luminometer will automatically name your protocol “New Protocol” and return to the Home Screen.
5393TA
Figure 5. A typical parameters screen.

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TM276 · Revised 5/21 www.promega.com
5.F. Kinetic Measurements
The kinetic measurement feature provides a method for monitoring real-time luminescence. The data point frequency
is the rate at which the measurements are taken for a particular luminescent reaction. Access the kinetic measurement
options from the Protocol Screen (Figure 5). Touch Kinetics to turn Kinetics on. Use the up and down arrow keys to
set the frequency of data point collection as well as the number of total data points. The integration time does not apply
when the kinetics feature is turned on. Be sure to connect the luminometer to a computer or printer using the RS-232
serial cable to collect the data points during a kinetic protocol. Touch OK to save your changes.
5.G. Default Protocol
Select Default Protocol to measure your sample for an integration of one second. Touch Default Protocol to jump
directly to the Home Screen.
5.H. Running a User Protocol
To access a saved protocol, select Run User Protocol. The next screen will display protocols previously saved on the
luminometer. Identify and touch the name of your protocol. The next screen will display the parameters of the saved
protocol for your review and adjustment. Touch OK to go to the Home Screen.
6. Measuring Your Samples
The GloMax®20/20 Luminometer is a bottom-reading luminometer and does not require a minimum sample volume.
The sample compartment of the luminometer accommodates 35mm petri dishes and 1.5ml microcentrifuge tubes with
a tube holder.
1. If using a microcentrifuge tube, open the lid of the GloMax®20/20 Luminometer and insert the Microcentrifuge
Tube Holder.
2. Place your sample in a microcentrifuge tube, and gently place the tube in the microcentrifuge tube holder. Be
sure that the microcentrifuge tube cap is open. Alternatively, you can place a petri dish directly into the sample
compartment.
3. Close the lid.
4. Optional: Touch Sample ID to name your sample. Using the keypad, enter the sample name into the name field
(Figure 6). You can enter a maximum of 10 characters. Touch Save to save the sample ID.
5. Touch Measure Luminescence once to begin the protocol sequence. To abort a measurement in progress,
touch the screen once.

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Notes:
Do not open the instrument lid while a run is in progress.
Touching any part of the screen during measurement will abort the reading.
The GloMax®20/20 Luminometer will report the Relative Luminescence Units (RLU) on the Home Screen. If you are
running a Dual-Luciferase®Reporter (DLR™) protocol, the ratio for each set of measurements will also appear. The
GloMax™ 20/20 Luminometer will display the status of the last 20 measurements. Use the arrow keys located in the
navigation bar to scroll through recent measurements. Note: Measurements are not saved after power down. The
instrument display will only show 20 measurements; additional measurements will overwrite the first measurements.
For more than 20 measurements, connect the instrument to a PC (Section 4.B).
Figure 6. You may name your sample from the Sample ID screen.
7. Saving Your Protocol
If you wish to store your protocol for future use, touch Save As from the Parameters Screen. The Save As screen
contains a keypad to name your protocol. Enter the name of your protocol in the Name field. You can enter a maximum
of 10 characters for your protocol name. Touch Save to store the protocol. You can access the saved protocols from the
Run User Protocol feature in Protocols. You may save up to 18 protocols at one time.
5396T
A
Figure 7. You can customize and save your protocol for future use.

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TM276 · Revised 5/21 www.promega.com
8. Tools
Use the Tools menu to access Settings and Diagnostics. Touch the Tools key to reach the menu.
8.A. Settings
Contrast
The screen contrast adjustment is located under the Settings menu. Use the left and right arrow keys to increase the
brightness of the touch screen. Touch Home when finished to return to the Home Screen.
Reset
The reset key is located under the Settings menu. It automatically power cycles the luminometer. The power cycle
erases any unsaved protocols and the data on the Home Screen. The reset key is an easy way to restart the luminometer
after inserting a Fluorescence Module or the Light Standard.
Lid Start
The lid start feature is also located under the Settings menu. When lid start is enabled, the luminometer begins
measurement immediately after closing the lid. Touch Lid Start once to enable the lid start feature. Return to the
Tools menu to disable the Lid Start feature.
8.B. Diagnostics
Touch Screen Calibration
Access the screen calibration procedure from the Diagnostics menu. Although the touch screen is calibrated at the
factory, it may need recalibration during instrument setup and over time. Contact Promega for more information on
screen calibration. If the touch screen appears nonresponsive and the Diagnostics menu cannot be activated, please see
the alternative instructions below.
1. Select Touch Screen Calibration.
2. When the screen reads “Please touch the calibration point”, touch the gray box on the + with a rounded object
like a pen cap, a stylus or the pointed end of a microcentrifuge or PCR tube. Do not use a pen or anything sharp
because this might damage the screen.
3. A second gray box will appear when the first + has been touched. Touch the +in this gray box.
4. Options for saving the calibration will appear. Touch Apply New Calibration if the new calibration is correct.
Select Reset To Factory Calibration or Abort Calibration if a mistake is made during the calibration process.
Alternatively, the touch screen can be reset to factory calibration settings by powering off the instrument and
then placing your finger on the screen during power on.
Keep your finger on the screen until the Home Screen appears. The touch screen should be reset to factory settings.
Device Configuration
The Device Configuration key contains information on the firmware version and other useful information for
troubleshooting instrument-related problems.

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
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8.C. Using the Light Standard
The optional Light Standard is a simple way to check the health status of the GloMax®20/20 Luminometer before
beginning measurement. The Light Standard contains three varying light levels to check the linearity and sensitivity of
the GloMax®20/20 Luminometer.
1. To use, turn the luminometer off.
2. Remove the Microcentrifuge Tube Holder from the sample compartment.
3. Insert the Light Standard into the sample compartment (Figure 8).
4. Turn the luminometer on. On the first use of the Light Standard, the Module Identification Screen will appear.
Identify the Light Standard. The luminometer will automatically power OFF. The GloMax®20/20 Luminometer
will automatically identify the Light Standard for subsequent Light Standard checks. If this is the first use, you
will need to turn the luminometer back on to proceed.
5. The GloMax®20/20 Luminometer will automatically begin the Light Standard protocol. Afterward, the
luminometer status will appear on the touch screen. If the status is “Good,” remove the Light Standard and
proceed with measurement. Otherwise, contact Promega for information.
8.D. Removing the Light Standard
1. To remove the Light Standard from the GloMax®20/20 Luminometer, turn the luminometer off.
2. Grasp and lift the red knob located in the center of the Light Standard to unlock it from the sample compartment.
Figure 8. Inserting the Light Standard into the Sample Compartment.
5399MA
Light Standard

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TM276 · Revised 5/21 www.promega.com
9. GloMax®20/20 Luminometer Fluorescent Module
The Fluorescent Module (Cat.# E5351 and E5361) enables measurement and analysis of common fluorophores. The
following Fluorescent Modules are available for different excitation and emission wavelengths:
• Blue: excitation, 460nm; emission, 515–575nm
• UV: excitation, 365–395nm; emission, 440–470nm
The type of Fluorescent Module required for a particular assay depends on the excitation and emission wavelengths of
the fluorophore or dye. The Fluorescent Module is designed for easy installation and removal. Store the Fluorescent
Module in the product case when not in use. Keep liquids, dust and debris away from direct contact with the Fluorescent
Module.
9.A. Installing the Fluorescent Module
1. Turn off the GloMax®20/20 Luminometer.
2. Align the metal pins of the Fluorescent Module with the module port inside the luminometer (Figure 9).
3. Slide the Fluorescent Module forward and press down to lock it into place.
4. Turn on the GloMax®20/20 Luminometer.
5. If this is the first-time use of the Fluorescent Module, you must identify the type of module. Touch the button
that corresponds to the name of the module. If you are not sure of the identification of your module, contact
Promega Technical Services.
Figure 9. Aligning the Fluorescent Module with the module port.
5399MA
Light Standard
Module Port
Fluorescent Module

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6. The GloMax®20/20 Luminometer will automatically turn off.
7. Turn on the luminometer with the module properly installed. The controls specific to the Fluorescent Module
will automatically appear on the touch screen.
8. Subsequent installation of the Fluorescent Module will automatically launch the proper touch screen controls.
9.B. Touch Screen Controls
Home Screen: From the Home Screen you can access Help, Tools, Calibration, Mode or Measure Fluorescence.
Touch the Help button to access a variety of help topics.
Mode: There are two measurement modes for the Fluorescent Module. The Raw Fluorescent mode provides a simple
method to measure the fluorescence of a sample without calibration. The Direct Fluorescent mode requires a calibration
with one standard and one blank. The Direct Fluorescent mode reports readings in one of several units (i.e., µg/ml,
ng/ml, pg/ml, ppb, ppt or FSU). With a valid calibration, it is not necessary to generate a standard curve to determine
the actual concentration of the sample.
Calibration: Touch Calibration to calibrate the Fluorescent Module with a known standard. Select the units of
measurement that correspond to the units of your standard. For example, if your standard is 200ng DNA/ml, select the
ng/ml option. Next, enter the final concentration of your standard. We recommend selecting a standard with a
concentration similar to a typical sample concentration. Subsequent sample readings reflect the actual concentration.
Measurement: Analysis with the Fluorescent Module requires the minicell cuvette. The maximum volume of the
minicell is 250µl. The minimum volume is 100µl. Insert the minicell cuvette into the Fluorescent Module. Close the lid
and touch the green Measure Fluorescence button on the touch screen. After a 5-second measurement time, the
result will appear on the touch screen next to the Sample ID.
9.C. Removing the Fluorescent Module
1. Turn off the GloMax®20/20 Luminometer.
2. Unlock the module by pulling up on the red knob. The module will disengage from the luminometer.
3. Carefully remove the Fluorescent Module and store it in the product case.

14 Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
TM276 · Revised 5/21 www.promega.com
10. Troubleshooting
For questions not addressed here, please contact your local Promega Branch Office or Distributor. Contact information
available at: www.promega.com. E-mail: [email protected]
Symptoms Causes and Comments
Luminometer not reporting measurements Integration time is set to “0” seconds. Touch Parameters
on the touch screen and increase the integration time.
Touch screen blank or not responsive Sample has saturated luminometer. Turn the luminometer off.
Wait 5 seconds, and then turn the luminometer on.
Fingerprints on touch screen Turn off, the luminometer then use a laboratory tissue dampened
with 70% ethanol to clean the touch screen.
Touch screen is misaligned, requiring selection Touch screen requires calibration. Turn off the GloMax®
outside the button area for the luminometer to 20/20 Luminometer. Touch the luminometer screen with one
respond finger. Keep the finger on the screen and turn the power on.
Continue touching the GloMax®20/20 Luminometer screen
until the Home Screen appears. (Also see Section 8.B, Touch
Screen Calibration.
Excel does not open. Excel is not installed on the PC. Make sure Microsoft®Excel is
installed on your PC and activated.
The GloMax®Software cannot find Excel on the PC. Open Excel
from the Programs menu on the PC and then open the GloMax®
Spreadsheet Interface Software.
Both green lights are on, but data fail to The wrong COM port is selected. Click Stop, then click on the
appear in Excel COM button to change the COM port.
Luminometer is not connected to the PC. Check the USB or
RS-232 connection between the luminometer and the PC. In
some cases, another program may have reserved the COM port
and locked out the GloMax®Spreadsheet Interface Software.
New data not reporting to Excel There is an editing process occuring within an Excel spreadsheet
cell. Save the data currentl displayed in Excel, then restart the
GloMax®Luminometer and begin collecting new data. In future,
wait until all the data are collected before editing the Excel
spreadsheet.
The software does not install. The PC allows only administrators to install new software. Log
in as Administrator, then install the software or contact your
internal computer support desk.
The software does not open. The software was not installed properly. Log in as Administrator.
Remove the software and re-install.

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
www.promega.com TM276 · Revised 5/21
11. Appendix
11.A. General Care
Proper care for the GloMax®20/20 Luminometer ensures accurate measurements and preserves sensitivity.
• Do not spill liquids into the sample compartment. Wipe up any spills immediately.
• Periodically wipe off the outside of the instrument with a cloth dampened in deionized water or 70% ethanol. Do
not use solvents or abrasive cleaners. Take caution when cleaning the touch screen. Use a laboratory tissue
dampened with 70% ethanol to remove fingerprints.
• Do not open the lid while a measurement is in progress. This action may damage the light detector.
11.B. Warranty and Service
The GloMax®20/20 Luminometer comes with a one-year warranty from Promega. Additional warranty is available.
For more information contact Promega Technical Services. Contact information is available on the web at:
www.promega.com. E-mail: [email protected]
To obtain service during the warranty period, please take the following steps:
1. Contact Promega Technical Services.
2. Carryout minor adjustments or tests as suggested by your Technical Services contact.
3. If it is determined that the instrument should be returned for repair, Promega Technical Services will arrange for
service by an authorized GloMax®service agent. You will be issued a Promega return authorization number.
Note: YOU MUST OBTAIN an Promega return authorization number before returning a luminometer for service.
4. Before returning the luminometer, you will be reponsible for cleaning it and providing a Certificate of
Decontamination (see Section 11.D)

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TM276 · Revised 5/21 www.promega.com
11.C. Specifications
Performance Specifications
Detection Limit: Better than 700 molecules of luciferase
Linear Dynamic Range: Greater than 8 decades
Sample Adaptor: Holds 1.5ml microcentrifuge tube
Detector: Bottom-reading Photomultiplier Tube (PMT)
Spectral Response Range: 350–650nm
Peak Wavelength: 420nm
Technical Specifications
Data Output: 100% ASCII format through a 9-pin RS-232 serial cable at 9600 baud or USB output
Spreadsheet Software Interface: Compatible with 32-and 64-bit PCs using Windows®7 Professional Service Pack
1 with either Microsoft®Office 2010 Home and Business Edition (32- and 64-bit versions) or Microsoft®Office 2007
(32-bit version)
Power: 0.5A @ 100–240V, 50–60Hz (universal)
Dimensions: 12.92 D × 10.44 W × 8.42” H (32.82 × 26.52 × 21.39cm)
Weight: 8.1 lb. (3.65kg)
Warranty: One year
CE Compliance: Pollution degree 2
Installation category II
Indoor use only
Mains supply voltage fluctuations are not to exceed ±10 percent of the
nominal supply voltage
This instrument complies with the requirements of the EU Directives for
electromagnetic compatibility (EMC Directive) and electrical safety (the LVD).
Compliance with the relevant Directives was demonstrated by third-party
testing to the appropriate EN and IEC standards. The product bears the CE
mark on its label as evidence of compliance, and an EU Declaration of
Conformity for the product is available from Promega on request.
Environmental Requirements
Shipping and Storage Conditions: 4–50°C, under noncondensing conditions, 20–75% humidity
Operating Conditions: 60–105 °F (15–40°C), 20–75% humidity
Operating Altitude Conditions: less than 2,000 meters above sea level

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
www.promega.com TM276 · Revised 5/21
11.D. Certificate of Decontamination
Disinfection and decontamination are required before shipping the instrument and instrument accessories for repair.
Instruments returned for service must be accompanied by a signed and dated Certificate of Decontamination, which
must be attached to the outside packaging of the instrument.
1. Clean the optical head according to the instructions in Section 11.A.
Failure to confirm disinfection and decontamination will result in decontamination charges before the
instrument will be serviced.
Select either (A) or (B):
A. I confirm that the returned items have not been contaminated by body fluids or by toxic, carcinogenic, radioac-
tive, or other hazardous materials.
B. I confirm that the returned items have been decontaminated and can be handled without exposing personnel to
health hazards.
Circle the type of material used in the instrument:
Chemical Biological Radioactive**
Briefly describe the decontamination procedure performed:
Date:
Place:
Signature:
Name (block capital letters):
** The signature of a Radiation Safety Officer is also required if the instrument was used with radioactive materials.
This instrument is certified by the undersigned to be free of radioactive contamination.
Date:
Place:
Signature:
Name (block capital letters):

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TM276 · Revised 5/21 www.promega.com
11.E. Related Products
GloMax®20/20 Accessories
Product Size Cat.#
GloMax® 20/20 Light Standard 1 each E5341
GloMax® 20/20 Fluorescent Module, UV 1 each E5351
GloMax® 20/20 Fluorescent Module, Blue 1 each E5361
GloMax® 20/20 Test Tube Holder, 1.5ml Tubes 1 each E5371
GloMax® 20/20 Replacement Power Supply 1 each E5411
Thermal, Serial Printer and Cable, Universal Power 1 each E2821
Tripp Lite USB to Serial Adapter 1 each TS2520
Microplate Readers
Product Size Cat.#
GloMax® Discover System (Mulmode Plate Reader) 1 each GM3000
GloMax® Navigator System (Microplate Luminometer) 1 each GM2000
Luminescent Assays
Please visit www.promega.com for complete listings of Luciferase Assays, Cell Health Assays and Cell Signaling Assays.
11.F. Summary of Changes
The following changes were made to the 5/21 revision of this document:
1. Updated Section 11.C, Specifications.
2. Incorporated other general edits.
3. Updated the cover page.

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www.promega.com TM276 · Revised 5/21
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