Quantum Turret 6 Installation guide

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22910EastApplewayAvenue,Suite4,LibertyLake,WA99019‐8606
Tel:(509)624‐9290•Fax:(509)624‐9488•E‐mail:[email protected]•Web:www.qnw.com
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Turret6
6‐PositionTemperature‐ControlledCuvetteTurretfor
UV/VisSpectrophotometry
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Manual&ProductOverview
3/18/2015
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Turret6 CuvetteHolder
TC1Temperature
Controller

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TABLEOFCONTENTS
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TableofContents....................................................................................................................................2
1.GETTINGSTARTED...........................................................................................................................3
A.YOURSHIPMENTWILLCONTAIN:..................................................................................................3
Turret6package..............................................................................................................................3
B.DEPENDINGONYOURORDER,THESHIPMENTMAYALSOCONTAIN:..........................................3
BATH10...........................................................................................................................................3
T‐Appsoftware................................................................................................................................3
C.SYSTEMSETUP................................................................................................................................4
D.SOFTWAREINSTALLATION.............................................................................................................4
E.SYSTEMOPERATION.......................................................................................................................4
2.UsingtheMenUBUTTON...................................................................................................................6
3.FeaturesoftheTurret6.....................................................................................................................8
A.GENERALDESCRIPTION..................................................................................................................8
B.CUVETTEHOLDER...........................................................................................................................9
C.CUVETTEZ‐HEIGHT.........................................................................................................................9
E.COOLINGWATERFORTHEPELTIER..............................................................................................10
F.DRYGASTOMINIMIZECONDENSATION.....................................................................................11
G.EXTERNALTEMPERATUREPROBE................................................................................................11
H.EXTERNALCOMPUTERCONTROL.................................................................................................11
4.ErrorCodes.......................................................................................................................................11
E5–celloutofrange..........................................................................................................................11
E6–celloutofrange.........................................................................................................................12
E7–heatexchangererror.................................................................................................................12
E8–inadequatecoolant....................................................................................................................12
5.Turret6Specifications......................................................................................................................13
Appendix:SerialCommunicationsfortheTC1TemperatureController.............................................14
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1. GETTINGSTARTED
ThankyouforpurchasingaQuantumNorthwestTurret 6.Wewantyoutoenjoymanyyearsof
faithfulservicefromyourinstrument.Ifyouhaveanyquestions,feelfreetocontactusdirectly
throughourwebsite:www.qnw.com.
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A.YOURSHIPMENTWILLCONTAIN:
Turret6package
1. Turret6cuvetteholder,mountedonabasefortheAgilentCary50orCary60
spectrophotometer
2. TC1TemperatureController
3. powercable
4. USBcable
5. 6magneticstirbars
6. vinyltubingtoconnectwaterandgas
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B.DEPENDINGONYOURORDER,THESHIPMENTMAYALSOCONTAIN:
BATH10
1. submersiblepumpadaptedto1/8‐inchIDtubing
2. plasticbucket
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T‐Appsoftware
T‐App,providedonaCD,isaprogramthatpermitsexternalcomputercontroloftheTC1
TemperatureController.Thetemperatureofthesampleholderandatemperaturesensedbyan
externalprobemaybeplottedvstime.Simpletextscriptsmaybeusedtoautomatemultiple
operations.

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C.SYSTEMSETUP
1. InstalltheTurret 6inyourspectrometer.(Itmayhavebeenalreadyinstalledbythe
manufacturer.)
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2. SupplycirculatingwatertothePeltierunit.IfyoupurchasedtheBATH10,thenattachtubing
fromthesubmersiblepumptoeitheroneofthewaterhosebarbsontheTurret 6.Attach
anotherpieceoftubingfromtheTurret 6backtothebucket.Putwaterinthebucket,turn
onthepumpbypluggingitintoanoutletandchecktobesurethatwaterisflowingbackinto
thebucket.Checkforleaks.Unplugthepumpuntilyouhavecompletedtheremainderof
thesystemsetup.
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3. Ifyouplantoworkatlowtemperatures,connectasourceofdrygas(typicallynitrogen)to
releaseonthecuvettewindows.Attachalengthoftubingwith1/16‐inchinsidediameter,to
thesmallhosebarbonthebaseoftheTurret 6.(A1/8to1/16‐inchbarbedreducerfitting
isincludedwiththeTurret 6,topreventanydifficultyconnectingtothisunusuallysmallsize
tubing.)Setthedrygasflowratetoabout50cc/min.
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4. TheTurret 6hastwoelectricalcables,onefordrivecurrentsandtheotherfor
sensitivesensorsignals.ConnectthemtothebackoftheTC1TemperatureController
onthe15‐pinconnectorslabeled“Sample”and“reference”.
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5. PlugthepowercableintothebackoftheTC1TemperatureControllerandintoawallsocket.
(TheTC1willacceptACvoltagesfrom85to264at50or60Hz.)
D.SOFTWAREINSTALLATION
NOTE:IFYOUPURCHASEDTHET‐AppPROGRAM,DONOTPLUGINTHEUSBCABLEINTOYOUR
COMPUTERUNTILTHESOFTWAREISLOADED.IFYOUDO,WINDOWSMAYAUTOMATICALLY
INSTALLANINCORRECTDRIVERTHATWILLBEDIFFICULTTOREMOVE.
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1.IfyoupurchasedtheT‐AppprogramforexternalcontroloftheTurret 6,inserttheCDinto
thedrive.Iftheinstallationdoesnotstartautomatically,locatetheSetup.exefileintheroot
directoryandrunit.Theinstallationprocessstartswithasmallblackwindowthatisshown
duringinstallationofthedriversneededtocontroltheTurret 6throughaUSBconnection.
Thiswindowwillthenbereplacedbythesoftwareinstallationwindow.Followtheonscreen
promptstocompletetheinstallation.
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2.ConnecttheTurret 6toyourcomputerusingtheUSBcableprovided.TheNewHardware
installationprocesswillbeginautomaticallyandtakeafewmomentstofinish.
E.SYSTEMOPERATION
1. Usealiquidsamplesinstandard1x1‐cmsquarecuvettesandplacethecuvettesinthe
Turret 6.
2. Placethemagneticstirbarineachcuvette.

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3. Ifyouwishtomonitorthetemperatureinsideacuvette,plugastandardSeries400orSeries
500thermistorprobe(notprovided)intothe¼‐inchphonejacklabeled“probe”intheback
paneloftheTC1.Placetheendoftheprobeinaregionofthesolution,whereitwillnot
occludethespectrometerlightbeam.
4. Turnonthecirculatingwater.
5. TurnontheTC1controllerusingtheswitchonthebackpanel.
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MenuButton
6. BriefInstructions:ThecontrollerdisplaybeginsontheDisplaypage.Usetheleftorright
arrowsontheMenuButtontocyclethroughthefivepagesofoptions,Display(thedefault
screen),SetTemperature,SetStirring,SetRamping,andSetPosition.Forexample,from
theDisplaypage,pushtheleftarrowoncetogettotheSetPositionpage.Oncethere,use
thetopandbottomarrowstochooseaposition,e.g.position5.PresstheSETbutton.The
cuvetteholderwillmovetoposition5andthemenuwillreturntotheDisplaypage.Toseta
temperature,presstherightarrowoncetogototheSetTemperaturepage.Oncethere,use
theupanddownarrowstosetatargettemperature,e.g.37.0°C.PressSETtoinitiate
temperaturecontrol.Thetemperaturewillriseandstabilize,indicatedbythechangeinthe
greenLEDlightonthefrontpanelfromflashingtosteady.Tocontrolstirring,gototheSet
Stirringpage.Usetheupanddownarrowstosetaspeed,e.g.1200rpm,andpressSETto
startthestirrer.MoredetailsontheMenuButtonareprovidedinSection2below.
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7. Aftermeasurementsarecompleted,turnoffpoweronthebackoftheTC1controllerand
turnoffthewatersource.
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2.USINGTHEMENUBUTTON
Usetherightandleftarrowstocyclethroughfivepages:
–Display–SetTemperature–SetStirring–SetRamping–SetPosition–
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Display
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Display:ThismainpageshowstheactualcuvetteHoldertemperature,theTargettemperatureanda
Probetemperature(ifaprobeispresent).ThepagealsoshowswhethertheRampandStirfunctions
areonoroff,andtheidentityofthecuvettePositionNumber(POSn,n=1to6)currentlyinthelight
pathway.Afterafewsecondsofinactivity,allotherpageswillrevertbacktotheDisplaypage.
Whenseekingatemperature,thegreenLEDonthefrontpanelwillflashslowly.Whenithaslocked
ontoanewtargettemperature,thegreenLEDwillremainlit.ArapidlyflashingredLEDusually
meansalooseelectricalcableorinadequatewaterflowforthePeltierunit.
Note:PressingtheSETbuttonwhiletheDisplaypageisactiveresultsinnoaction.Tostartorstop
controllerfunctions,accessthefourotherpages,SetTemperature–SetStirring–SetRamping
–SetPosition.
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SetTemperature
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SetTemperature:TosettheTargettemperature,usetheupanddownarrows.PressSETtoretain
thisnewTargetandinitiatetemperaturecontrol.ThegreenLEDlightwillbeginflashingslowlyasthe
deviceseeksthetargetedtemperature,andthepagewindowwillshowtheCurrenttobeON.
Holder = 37.0 °C POS
Target = 37.0 °C 1
Probe = 36.9 °C
Ramp On Stir On
Set Temperature
Target = 37.0 °C
Current= 23.6 °C Off

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DiscontinueTemperatureControl:PressSETwhilethegreenLEDlightislitorslowlyflashingto
discontinuetemperaturecontrol.TheLEDlightwillturnoffcompletely,andthepagewindowwill
showtheCurrenttobeOff.
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SetStirring
SetStirring:ToturnonmagneticStirring,usetheupanddownarrowstochooseanapproximate
stirringspeedbetween1and2500rpm.PressSETtosetthespeedandinitiatestirring.Thepage
willupdatetoshowtheCurrentvaluetobethesameasStirspeed.
DiscontinueStirring:PressSETwhiletheStirringison(shownbynonzeroCurrentrpm)toturnthe
stirreroff.ThepagewillupdatetoshowtheCurrentspeedtobeOff.
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SetRamping
SetRamping:Toperformatemperatureramp,settheRamprateusingtheupanddownarrows,
andpressSET.ThepagewillupdatetoshowtheCurrentratein°/mintobethesameastheRamp
rate.Withrampingset,turningontemperaturecontrolwillgeneratealinearramptothetarget
temperature.Whenthesampleholderreachesthetargettemperature,nofurthertemperature
changeoccurs,althoughtheCurrentratewillremainasset.
Thefastestpossiblerampisdeterminedbyhowfastthecuvetteholdercouldreachthetarget
temperaturewithoutramping.Attemptingtoramptooquickly,especiallyathighandlow
temperatureextremes,willresultinanonlinearramp.Theslowestrampthatmaybesetonthis
pageis0.01°C/minute.(Ifneeded,muchslowerrampsmaybesetthroughsoftwarecommands.)
DiscontinueRamping:PressSETwhiletheRampison(shownbynonzeroCurrent°/min)toturn
theRampoff.ThepagewillupdatetoshowtheCurrentratetobeOff.

Set Stirring
Stir speed = 1200 rpm
Current = Off
Set Ramping
Ramp = 0.55°/min
Current = Off

8

SetPosition

SetPosition: Tochangethecuvetteposition,usetheupanddownarrowstochoosethedesired
Targetpositionandpress
SET .Thecontrollerwillmovethecuvetteholdertotheselectedcuvette
position,reflectedbythevalueofCurrent.
Whenthecuvetteholderispoweredon,thedevicehomestoastartingpointbeforeseekingthe
targetedcuvetteposition.Tomanuallyhomethedevice,pushandholdthe
SET buttonforthree
seconds.Thiswillhomethecuvetteholderbeforemovingthedevicetothetargetedposition.

3.FEATURESOFTHETURRET6
A.GENERALDESCRIPTION
TheTurret 6hasarotating,temperature‐controlled,cuvettetowerwhichholdsuptosixcuvettes.
Thecenterofthetowerishollow,permittinglighttopassintotheentrancehole,throughthetower,
throughacuvetteandexittheoppositesidefromtheentrance.Aplasticcoverenclosesthecuvette
tower,providingthermalinsulation.Afloorisattachedtothebottomofthecuvettetower,andthe
rotatingbodyoftheholdercontainsaPeltierelementsandwichedbetweenthisfloorandabrass
heatexchanger.Waterisputthroughtheheatexchangertodrawoffheatgeneratedwhenthe
temperateislowered.

Figure1.Turret 6
Aminiaturesteppingmotorisplacedundereachcuvettetodriverotatingmagnetandthesesix
motorsrotatewiththeTurretbody.Drygasmaybedirectednexttothecuvettewindows,bothon
theinsideandtheoutsidesurfaces,tominimizecondensationwhenworkingbelowthedewpoint
Set Position
Target = 5
Current = 1

9

temperature.Therotatingbodyismountedonalargeprecisionbearingandrotatedviagears,using
asteppingmotor.


Figure2.BackoftheTurret 6

Twowiresexitthesteppingmotorhousing,oneconductsthedrivecurrentsforthesteppingmotor
andPeltierunit.Theotherconductsthemoresensiblesensorlines.Thetwobrass1/8‐inchhose
barbsforwaterprovideaccesstotheheatexchanger.A1/8‐inchstainlesshosebarbisusedforthe
drygas.Thehosestotheseconnectionsandthepowerandsensorcablemustbebroughtoutofthe
samplecompartmentbyameansthatdoesnotinterferewiththespectroscopicmeasurement.
Accessfortheseutilitiesmayneedtobelighttight.

CAUTION: Accidentlyattachingawaterlinetothedrygasbarbwillseverelydamagethe
instrument!Attachthelineswithcare.
B.CUVETTEHOLDER

TheTurret 6holdsstandard10x10mmcuvetteswithoutsidedimensionsof12.5x12.5mm.A
metalclipisusedtopusheachcuvetteintooneofthecornersofitsindividualspaceforreproducible
positioningandtofavortemperaturetransfer.WallsoftheTurretbodyarerelievedtoprevent
scratchingoftheopticalsurfacesofthecuvette.

Cuvettesshouldbe45mmortaller,otherwisetheywillbedifficulttorecoverfromthecuvette
holders.
C.CUVETTEZ‐HEIGHT
The“zheight”ofacuvetteisthedistancebetweenthebottomsurfaceofthecuvetteandthe
designedpositionfortheopticalcenterline,wheretheincidentbeamoflightstrikesthecuvette.
Thezheightforcuvettesinthe
Turret  6 is15mm.


10


Figure3.Thezheightofatypicalmicrocuvette
E.COOLINGWATERFORTHEPELTIER
ThePeltierelement(or“thermoelectriccooler”)isaheatpump.Whencooling,ittransfersheat
fromtheTurretbodytoaheatexchanger;whenheating,electricalpolarityisreversedandit
transfersheatfromtheheatexchangertothetower.Whencooling,itisparticularlyimportantto
transferthisheatawayfromthePeltier.Thisisaccomplishedwithflowingwaterthroughtheheat
exchanger.

Asourceofwater(orothercoolingfluid)mustbeconnectedtothe⅛‐inchhosebarbsonthebackof
theTurret 6.IfyoupurchasetheBATH10fromus,youwillgetaninexpensivesubmersible
aquariumpump,theappropriatefittingsforconnectingtubing,andaplasticbucket.Connectthe
pumptotheTurret 6,placeitinthebucketwithwater,andrunareturntubetothebucket.Amore
robustpumpisavailableastheBATH100forahigherprice,althoughthelargerpumphasahigher
Wattageandtendstoheatthewaterwhenusedforextendedperiodsoftime.

Youmayalsoprovidethecoolingwaterfromanothersource,suchasarefrigeratedcoolingbathor
evenatapforbriefuse.

Youwillneedaflowof100‐300ml/minute.Thisflowshouldrequireapressureofabout3‐5psi
(0.2‐0.3bar).Donotexceedaninputwaterpressureof25psi(1.7bar),asdamagemayoccurinside
theTurret 6.Theheatexchangerandhosebarbsarebrass,andthetubinginsidetheTurret 6is
vinyl.Besurethatanycirculatingfluidused,otherthanwater,willnotcorrodethesematerials.

ThetemperatureoftheheatexchangerintheTurret 6ismonitoredusingathermistor.Ifthe
temperatureexceeds60°C,thentemperaturecontrolisshutdowntopreventdamagetothePeltier
elementandthewarning,“checkcoolantflow,”displayedontheTC1temperaturecontroller.This
willhappenifthecirculatingfluidgetstoowarmand/orisrestrictedinflow.Theheatexchanger
temperaturemaybeaccessedbycomputerthroughtheRS232orUSBconnectionsonthebackof
theTC1TemperatureController(seeExternalcomputercontrolbelow).

Temperatureincreaseswillbefasterwhenroomtemperaturewaterisusedinthecirculator.
Temperaturedecreaseswillbefasterwhenicewaterisused.Onlywatershouldbecirculatedusing
theBATH10.Whenusingarefrigeratedbath,circulatingpre‐cooledfluids(suchas30%methanolor
dilutedethyleneglycol)atbelow0°Cwillpermitmeasurementsbelowthespecifiedtemperature
range.