Quantum turret 6/FO Installation guide

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22910EastApplewayAvenue,Suite4,LibertyLake,WA99019‐8606
Tel:(509)624‐9290•Fax:(509)624‐9488•E‐mail:[email protected]•Web:www.qnw.com
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turret6/FO
6‐PositionTemperature‐ControlledCuvetteTurretfor
FiberOpticSpectroscopy
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Manual&ProductOverview
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turre
t
6 CuvetteHolder
TC1Temperature
Controller

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TABLEOFCONTENTS
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1.GettingStarted....................................................................................................................................3
A.YourShipmentWillContain:..........................................................................................................3
B.SystemSetup..................................................................................................................................4
C.LensSystemInstallation.................................................................................................................4
D.SoftwareInstallation......................................................................................................................7
E.SystemOperation...........................................................................................................................7
F.UsingtheMenuButton..................................................................................................................8
2.Featuresoftheturret6....................................................................................................................10
A.GeneralDescription......................................................................................................................10
B.Cuvettes........................................................................................................................................11
C.Cuvettez‐Height...........................................................................................................................11
D.CoolingWaterforthePeltier.......................................................................................................11
E.DryGasPurging...........................................................................................................................12
F.ExternalTemperatureProbe........................................................................................................12
G.ExternalComputerControl..........................................................................................................12
H.DescriptionofLensSystems........................................................................................................12
3.ErrorCodes........................................................................................................................................14
4.SpecificationsSummary....................................................................................................................15
Appendix1.TemperatureSpecificationsfortheturret6.....................................................................16
Appendix2.SerialCommunicationsfortheTC1TemperatureController..........................................19
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1.GETTINGSTARTED
ThankyouforpurchasingaQuantumNorthwestturret 6/FO!Wewantyoutoenjoymanyyearsof
faithfulservicefromyourinstrument.Ifyouhaveanyquestions,feelfreetocontactusdirectly
throughourwebsite:www.qnw.com.Acopyofthismanualcanbefoundonthesiteunderthe
SUPPORTtab.
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A.YourShipmentWillContain:
turret6package
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1. turret6Temperature‐ControlledCuvetteHolder
2. TC1TemperatureController
3. BATH10SubmersiblePumpandBucket
4. powercable
5. USBcable
6. turret6accessorykit(slits,wrench,blanks,and6magneticstirbars)
7. vinyltubingtoconnectwaterandgas
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DependingonYourOrder,YourShipmentMayContain:
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LensSystems
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1. twot6‐QCLCollimatingLensforabsorbancemeasurements
2. t6‐QIL/exImagingLensforExcitation
3. t6‐QIL/emImagingLensforEmittedLight
4. t6‐QMPMirrorPlug
5. Plasticcapforcoveringunusedports
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T‐Appsoftware
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T‐App,providedonaCD,isaprogramthatpermitsexternalcomputercontroloftheTC1
TemperatureController.Thetemperatureofthesampleholderandatemperaturesensedbyan
externalprobemaybeplottedvstime.Simpletextscriptsmaybeusedtoautomatemultiple
operations.

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B.SystemSetup
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1. Placetheturret 6onatable.Forstabilityyoumaywishtousescrewstoattachtheturret
6,usingtheholesintheedgeofthebase.
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2. PlugthepowercableintothebackoftheTC1TemperatureControllerandintoawallsocket.
(TheTC1willacceptACvoltagesfrom85to264at50or60Hz.)
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3. SupplycirculatingwatertothePeltierunit.IfyouchoosetousetheBATH10,thenattach
1/8‐inchIDtubingfromthesubmersiblepumptoeitheroneofthewaterhosebarbsonthe
turret 6.Attachanotherpieceoftubingfromtheturret 6backtothebucket.Directionof
flowisnotimportant.Putwaterinthebucket.Plugthepump’scircularDINconnectorinto
the12VoltoutletonthebackoftheTC1labeled“IO.”BrieflyturnontheTC1andcheckto
besurethatwaterisflowingbackintothebucket.Checkforleaks.
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4. Ifyouplantoworkatlowtemperatures,connectasourceofdrygas(typicallynitrogen)to
releaseonthecuvettewindows.Attachalengthoftubingwith1/16‐inchinsidediameter,to
thesmallhosebarbonthebaseoftheturret 6.(A1/8to1/16‐inchbarbedreducerfittingis
includedwiththeturret 6,topreventanydifficultyconnectingtothisunusuallysmallsize
tubing.)Setthedrygasflowratetoabout50cc/min.
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5. Theturret 6hastwoelectricalcables,onefordrivecurrentsandtheotherfor
sensitivesensorsignals.ConnectthemtothebackoftheTC1TemperatureController
onthe15‐pinconnectorslabeled“Sample”and“Reference”.
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6. Insertopticsandattachsourcesanddetectorsasneededbyyourexperiment.Optical
optionsaredescribedbelow.
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C.LensSystemInstallation
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Therearetwobasicopticalconfigurationsfortheturret 6:onetomeasureabsorbanceandthe
othertomeasurefluorescence.
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Figure1.Absorbanceconfigurationwithcollimatinglensesoneachsideofthecuvette
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AsshowninFigure1,tosettheturret 6 upforabsorbancemeasurementsinsertcollimatinglens
(QCL/t6)atpositions1and3andscrewintoplace.Putablackplasticcapfromtheturret 6
accessorykitinposition2.Thecollimatingopticsproducea5mmdiameterbeamthatpasses
throughthecuvettecenteredat8.5mmabovetheoutsidebottomsurface.Attachfiberopticsas
describebelow.Typically,lightispassedfromposition1throughtheopticalslitandcuvetteand
collectedatposition3.Iftheslitisnotbeingused,directionisnotimportant.
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Figure2.Fluorescenceconfigurationwithexcitationandemissionthroughimaginglensesanda
mirrortoreflecttheincidentlightbackonitself

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AsshowninFigure2,tosettheturret 6 upforfluorescencemeasurementsinserttheimaginglens
forexcitation(t6‐QIL/ex)intoposition1.Thislensacceptsthelightfromafiberusinganumerical
apertureof0.22andfocusesanimageoftheendofthefiberintothecenterofthecuvetteusinga
magnificationof2.7.Inserttheimaginglensforemission(t6‐QIL/em)inposition2.Thislensimages
thecenterofthecuvetteontotheendofthecollectionfiberusingamagnificationof1.0.To
enhancecollectionefficiency,asphericalmirror(60mmradiusofcurvature)maybeplacedin
position3toreflecttheexcitationsourcebackonitself.
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Figure3‐Componentsofalensassembly
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1. Usethe5/64‐inchhexscrewdriverfromtheturret 6 accessorykittoloosenthesetscrew
(Figure3).
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2. Removethesilversleevewithablackendpiecefromthelensassembly.Setitaside.
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3. Screwtheemptyopticalassemblyallthewayintotheappropriatepositionintheturret 6.
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4. Lookforthelocationofthesetscrew.Ifitisinadifficultpositiontoreach,removethethree
fibersteeringscrews,rotatetheendoftheopticalassemblyuntilthesetscrewisaccessible,
andreattachthealignmentassemblysothescrewismoreavailable.Thesteeringscrews
compressanO‐ring,permittingfinealignmentoftheendofthefiberrelativetothelens.
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Figure4‐AttachingthefiberoptictotheSMAconnector

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5.Now,unscrewthesilversleevefromtheblackendpiecerevealingtheSMAconnector
(Figure4).
6.Slidethesilversleeveovertheendofthefiberopticconnectorandcable,leavingthe
threadedendtowardtheendofthecable.
7.AttachthefiberopticcabletotheSMAconnector.
8.Holdingtheblackendpiece,screwthesilversleevebackintoplace.
9.InserttheSMAconnectorandsilversleeveintotheholeintheopticalassembly.Forafirst
adjustment,inserttheassemblyuntilthealignmentgrooveisevenwiththefaceofthelens
assembly.Later,youcanoptimizethesignalintensitybyslidingtheSMAconnectorand
silversleeveindeeper.
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D.SoftwareInstallation
NOTE:IFYOUPURCHASEDTHET‐AppPROGRAM,DONOTPLUGINTHEUSBCABLEUNTILTHE
SOFTWAREISLOADED!IFYOUDO,WINDOWSMAYAUTOMATICALLYINSTALLANINCORRECT
DRIVERTHATWILLBEDIFFICULTTOREMOVE.
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1.IfyoupurchasedtheT‐Appprogramforexternalcontroloftheturret 6,inserttheCDinto
thedrive.Iftheinstallationdoesnotstartautomatically,locatetheSETUP.exefileintheroot
directoryandrunit.Theinstallationprocessstartswithasmallblackwindowthatisshown
duringinstallationofthedriversneededtocontroltheturret 6throughaUSBconnection.
Thiswindowwillthenbereplacedbythesoftwareinstallationwindow.Followtheonscreen
promptstocompletetheinstallation.
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2.Connecttheturret 6toyourcomputerusingtheUSBcableprovided.TheNewHardware
installationprocesswillbeginautomaticallyandtakeafewmomentstofinish.
E.SystemOperation
1. Useliquidsamplesinstandard1x1‐cmsquarecuvettesandplacethecuvettesintheturret
6.
2. Placeamagneticstirbarineachcuvette.
3. Ifyouwishtomonitorthetemperatureinsideacuvette,plugastandardSeries400orSeries
500thermistorprobe(notprovided)intothe¼‐inchphonejacklabeled“probe”intheback
paneloftheTC1.Placetheendoftheprobeinaregionofthesolution,whereitwillnot
occludethespectrometerlightbeam.
4. TurnontheTC1controllerusingtheswitchonthebackpanel.
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MenuButton
5. Thecontrollerdisplaybeginsonthe
Start page.UsetheleftorrightarrowsontheMenu
Buttontocyclethroughthefivepagesofoptions.Onthe
SetPosition pageusethetopand
bottomarrowstochooseaposition,sayposition5.Presssetandtheturretwillrotateto
thispositionandthemenuwillreturntothe
Start page.Tosetatemperature,gotothe Set
Temperature pageandusetheupanddownarrowstosetatargettemperature,say37.0
°C.Press
SET toinitiatetemperaturecontrol.Thetemperaturewillriseandstabilize.The
menuwillthenadvancetothe
SetStirrer page.Usetheupanddownarrowstosetaspeed,
say1200rpm,andpress
SET tostartthestirrer.
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6. Whentemperaturecontrolisrunningandthe
Start pageisbeingdisplayed,press SET to
turnofftemperaturecontrol.

7. Aftermeasurementsarecompleted,turnoffpoweronthebackoftheTC1controllerand
turnoffthewatersource.

F.UsingtheMenuButton
Usetherightandleftarrowstocyclethroughfivepages:
–Display–SetPosition–SetTemperature–SetStirring–SetRamping–


Display: ThismainpageshowstheactualcuvetteHoldertemperature,theTargettemperatureanda
Probetemperature(ifaprobeispresent).Thepagewillalsoshowthecurrentturretposition.After
afewsecondsofinactivity,allotherpageswillswitchbacktothe
Display page.
Whenseekingatemperature,thegreenledonthefrontpanelwillflashslowly.Whenithaslocked
ontoanewtargettemperature,thegreenledwillremainlit.Arapidlyflashingredledusuallymeans
alooseelectricalcableorinadequatewaterflowforthePeltierunit.

Holder = 37.0 °C
Target = 37.0 °C
Probe = 36.9 °C
Current Position = 5

9
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
SetPosition:Torotatetheturret6,usetheupanddownarrowstochoosethenewpositionand
pressSET.ThecontrollerwillreturntotheDisplaypage.


SetTemperature:TosettheTargettemperature,usetheupanddownarrows.PressSETtoretain
thisnewTargetandinitiatetemperaturecontrol.

SetStirring:Toturnonmagneticstirring,usetheupanddownarrowstochooseanapproximate
stirringspeedbetween1and2500rpm.PressSETtoturnonstirringatthisspeed.

SetRamping:Toperformaramp,settheRampingrateusingtheupanddownarrows,andpress
SET.Withrampingset,turningontemperaturecontrolwillgeneratealinearramptothetarget
temperature.Whenthesampleholderreachesthetargettemperature,therampratewillbeset
backto0°/minute.
Thefastestpossiblerampisdeterminedbyhowfastthecuvetteholdercouldreachthetarget
temperaturewithoutramping.Ifyouattempttoramptooquickly,especiallyathighandlow
temperatureextremes,youwillobtainanonlinearramp.Theslowestrampthatmaybesetonthis
pageis0.01°C/minute.(Ifneeded,muchslowerrampsmaybesetthroughsoftwarecommands.)
Set Ramping
Ramp = 0.55 °/min
Current = 0 °/min
Set Stirring
Stir Speed = 1200rpm
Current = 0rpm
Set Temperature
Target = 37.0 °C
Current= 20.0 °C
Set Position
Position = 5
Current Position = 1

10

2.FEATURESOFTHEturret6
A.GeneralDescription
Theturret 6hasarotating,temperature‐controlled,metalcuvettetowerwhichholdsuptosix
cuvettes.Aninsulatingcoverofurethaneplasticenclosesthecuvettetower,providingthermal
insulation.Afloorisattachedtothebottomofthetower,andtherotatingbodyoftheholder
containsaPeltierelementsandwichedbetweenthisfloorandabrassheatexchanger.Waterflows
throughtheheatexchangertodrawoffheatgeneratedwhenthetemperateofthecuvettetoweris
lowered.



Figure5.turret 6 withasectioncutawaytoshowtheopticaccesstooneofthecuvettes
Aminiaturesteppingmotorresidesundereachcuvettetodriverotatingmagnet.Drygasentersa
1/16‐inchhosebarbonthefrontoftheinstrumentandiscarriedthroughamanifoldinthefloorof
theturrettosmallholesonthebottomofthe18cuvettewindows.Thisflowofgaswillkeepthe
windowsfreeofcondensationwhenworkingbelowthedewpointtemperature.Therotatingbodyis
mountedonalargeprecisionbearingandrotatedviagears,usingtheexternalVextasteppingmotor
(notvisibleinthisview).

TwowiresexitbelowtheVextasteppingmotor.Oneconductsthedrivecurrentsforthestepping
motorandPeltierunit.Theotherconductsthesensorlines.Thetwobrass1/8‐inchhosebarbsfor
waterprovideaccesstotheheatexchanger.A1/16‐inchbrasshosebarbisusedforthedrygas.

CAUTION: Accidentlyattachingawaterlinetothedrygasbarbwillseverelydamagethe
instrument!Attachthelineswithcare.