Labnet ENDURO GEL XL E0160 User manual

Lit M00078 Rev 3; September 2016
ENDURO™ GEL XL
User Manual
HORIZONTAL GEL ELECTROPHORESIS UNIT
E0160
E0160-230V
E0160-230V-UK
E160-CAN

About This Manual
This manual is designed to assist you in the optimal usage of your Enduro
GelXL. The manual is available in English, French, German, Italian, Portuguese,
and Spanish on our website at www.labnetinternational.com/document-center

TABLE OF CONTENTS
I. MAINTENANCE 1
II. OPTIONS AND SPECIFICATIONS 2
A.
Components and Accessories 2
B.
Specifications 3
III. OPERATING INSTRUCTIONS 3
A.
Preparation of the Agarose Gel and Electrophoresis Buffer – DNA 3
B. Preparation of the Agarose Gel and Electrophoresis Buffer - RNA 5
C.
Casting the Gel 6
D. Removing the Comb 7
E. Loading the Samples onto the Gel 8
F.
Electrical Connections to the Safety Lid 8
G. Sample Electrophoresis 9
H.
Detection and Documentation of Separated Fragments 10
IV APPENDICES 11
A. Buffers for Electrophoresis 11
B. Physical Properties of Electrophoretic Plastics 12
V REFERENCES 12
DECLARATION OF CONFORMITY 17
WARRANTY 18

1
I. MAINTENANCE
Please handle the unit with care:
Do not expose the unit or its accessories to temperatures above
60°C.
Do not expose the unit to organic solvents.
Do not clean the unit with abrasive cleaners or cleaning aids.
In most cases, rinsing with deionized water will sufficiently clean the unit.
For heavier dirt, use a mild cleansing solution such as dish soap (alkaline
cleansers are not recommended). Hand wash and dry with a soft cloth.
To remove residual ethidium bromide, occasionally soak the unit in 1%
commercial bleach solution for 16 hours. Rinse well.
PLEASE NOTE: The degradation of acrylic due to solvents may result in
substantial discoloration, cracking, warpage, or etching of the
electrophoresis unit.
Do not apply any of the following solvents: benzene, xylene,
toluene, chloroform, carbon tetrachloride, alcohols, phenols, ketones,
or esters.
Do not expose the ABS combs supplied with this unit to
formaldehyde for extended periods. When casting gels containing
formaldehyde, remove the combs promptly upon hardening of the gel
and rinse completely with deionized water.
Elimination of RNase Contamination
Should treatment of the unit to eliminate RNase contamination be desired, clean
the unit with a mild detergent as described above followed by soaking for 10
minutes in a solution of 3 % hydrogen peroxide, and then for 1 hour in 0.1 % DEPC
(diethyl pyrocarbonate). Pour out final rinse, and air dry.
CAUTION: DEPC is a suspected carcinogen; handle with care.
Alternatively, soak the unit and accessories in freshly made 2.2 mM acetic
anhydride treated water (200 µl/liter) for at least five minutes. Solutions for RNA
work (electrophoresis buffers, etc) may be made from the same acetic anhydride
treated water as well.

2
WARNINGS:
CAUTION! Injury, damage to equipment, or property, may result if used in a
manner not specified by the manufacturer.
CAUTION! A pinch hazard exists between the plastic enclosure and the shaking
head.
CAUTION! NOT for use with flammable liquids.
II. OPTIONS AND SPECIFICATIONS
A. Components and Accessories
Catalog # Description
E0160 ENDURO Gel XL Complete Electrophoresis System
Comes complete with 1) 12.5 x 12 cm, 2) 12.5 x 6 cm UV Transmittant casting
trays, casting stand with divider, and four 1.0 mm thick 28/14 reversible tooth
combs, power cord and manual.
Accessories
Catalog #
Description
E0161
(1)12.5 x 12cm UV Transmittant Casting Tray
E0162
(2) 12.5 x 6 cm UV Transmittant Casting Tray
E0163
(4) 6 x 6cm UV Transmittant Casting Tray
E0164
(2) 1 mm x 14/28 tooth Reversible Comb
E0165
(
2) 1mm 5/8 tooth Reversible comb
E0166
Micro casting set - (4) 6 x 6cm UV Transmittant Casting Tray, 2) 1mm
5/8 tooth Reversible combs, Casting stand with divider
E0167
Casting Stand with divider
E0168
Standard casting set – (1) 12.5 x 12cm tray, (2) 12.5x6cm trays, (4)
14/28 tooth multi-channel compatible combs, casting stand with
divider
R1000
-
100BP
Molecular Weight marker 100 bp
R1000
-
1KB
Molecular Weight marker 1 Kb
B. Specifications
Unit dimensions 24.5 x 17.0 x 6.2 cm
Gel dimensions 12.5 x 12.0 cm
Maximum sample capacity: 112 samples (4 combs, 26
samples each)
Buffer Capacity: 300 ml
Distance Between Electrodes: 13.5 cm
Electrophoresis Tank
Overall dimension 18.3 × 16.4 × 5.6 cm
Material characteristic UV transmitting (50% at 254nm,
80% at 312nm)
Solution volume 300ml (includes buffer and gels)
Safety Lid
Overall dimension 19.7 × 16.9 × 3.8 cm
Material characteristic UV non-transmitting Polycarbonate