SCIEX P/ACE MDQ Plus Operating manual

P/ACE™ MDQ Plus Capillary
Electrophoresis System
System Maintenance Guide
RUO-IDV-05-4904-A
January 2019

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P/ACE™ MDQ Plus Capillary Electrophoresis System System Maintenance Guide
2 / 88 RUO-IDV-05-4904-A | B54955AB
AB Sciex LLC
500 Old Connecticut Path
Framingham, Massachusetts 01701
USA

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Contents
Chapter 1 Installation Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Install the UV or PDA Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Install Wavelength Filters for the UV Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Calibrate the PDA Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Install the LIF Detector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Installation Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Install the LIF Detector Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Install the LIF Detector in the P/ACE™ MDQ Plus Instrument . . . . . . . . . . . 18
Connect the Probe to the Clamp Bar. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Calibrate the LIF Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Troubleshoot the LIF Detector Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Install the Capillary Cartridge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Rebuild a Capillary Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Install the Capillary Cartridge into the P/ACE™ MDQ Plus Instrument . . . . . 42
Install the Universal Vials and Caps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Fill the Universal Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Fill the Micro Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Chapter 2 Maintenance Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Clean the Ejecting Levers, Electrodes, and Interface Block. . . . . . . . . . . . . . . . . 48
Remove the Ejecting Levers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Remove the Electrodes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Replace the Electrodes in the Interface Block . . . . . . . . . . . . . . . . . . . . . . . . . 52
Replace the Ejecting Levers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Refill the Capillary Coolant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Clean the Fiber Optic Cable (UV and PDA Detectors) . . . . . . . . . . . . . . . . . . . . 54
Replace the Deuterium Lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Procedures for Instrument Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Replace the Quad Rings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Replace the Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Procedures for LIF Detector Care and Maintenance . . . . . . . . . . . . . . . . . . . . . . 65
Inspect the LIF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Clean the LIF Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Store the LIF Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Chapter 3 Connect the System to a Mass Spectrometer . . . . . . . . . . . . . . . . . . . 67
About CE-MS Mode. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Install the Capillary in the Standard Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Install the Capillary in the EDA-Only Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . 71

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Install the Ground Connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73
Chapter A Evaluating UV Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75
Chapter B Performance Tests for the LIF Detector . . . . . . . . . . . . . . . . . . . . . . .79
Relative Fluorescent Units (RFU) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
Calibration Correction Factor (CCF). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
Applying the CCF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Automatic Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Calibration Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Calibration Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82
Non-fluorescent Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82
LIF Calibration for Other Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
LIF Calibration for Different Fluorescent Solutions . . . . . . . . . . . . . . . . . . . . . . .83
Standard System Performance Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84
Calibration Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84
Calibration Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Contact Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Customer Training. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Online Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
SCIEX Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
CyberSecurity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Documentation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88

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CHAPTER 1
Installation Procedures
CAUTION
Prior to using the system, refer to the safety content in the System Overview Guide
for detailed information on the safe use and operation of the system.
This chapter contains the following installation procedures for the SCIEX P/ACE™ MDQ Plus
capillary electrophoresis system. Begin by installing the detector, and then continue to remaining
items.
• Install the UV or PDA Detector on page 6
• Install Wavelength Filters for the UV Detector on page 9
• Calibrate the PDA Detector on page 14
• Install the LIF Detector on page 15
• Install the Capillary Cartridge on page 24
• Install the Universal Vials and Caps on page 44
WARNING
Disconnect the power before any instrument disassembly. Failure to do
so can cause electrical shock or other injury.
WARNING
Maintenance or repair procedures not specifically described in this
guide present a risk of electrical shock or injury. Refer additional
servicing to SCIEX.
WARNING
Do not attempt to defeat any of the instrument interlocks or safety
mechanisms.

P/ACE™ MDQ Plus Capillary Electrophoresis System System Maintenance Guide
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Install the UV or PDA Detector
Required Materials
• UV or PDA detector
• Fiber optic cable (UV cable is 2-ended and the PDA cable is 3-ended)
1 Verify the UV source optic module is installed.
2 Verify that power is off and no capillary cartridge is installed.
3 Lift the cartridge cover door and find the detector mounting location as shown in Figure 1.1
(for a UV detector) and Figure 1.2 (for a PDA detector).
4 Remove the detector from the package and remove the protective connector caps.
5 Position the detector on the mounting location. Carefully slide the detector back to make
contact with the back-plane receptacle.
6 Tighten the thumbscrew until tight.
7 Unpack the fiber optic cable and remove the protective covers from each end (two covers for
the UV cable and three covers for the PDA cable). Make sure to keep the protective covers
for storing the cable when it is not in use.
8 Connect the cable to the clamp arm first, then to the detector.
9 Depending on the detector, either:
• If needed, Install Wavelength Filters for the UV Detector on page 9.
• Calibrate the PDA Detector on page 14.

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Figure 1.1 P/ACE™ MDQ Plus Instrument–Front View With UV Detector Installed
1. Outer Door Cover
2. UV Detector
3. Two-ended Fiber Optic Cable
4. Clamp Bar
5. Capillary Cartridge
6. Interface Block

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Figure 1.2 P/ACE™ MDQ Plus Instrument–Front View With PDA Detector Installed
1. Outer Door Cover
2. PDA Detector
3. Three-ended Fiber Optic Cable
4. Clamp Bar
5. Capillary Cartridge
6. Interface Block

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Install Wavelength Filters for the UV Detector
The P/ACE™ MDQ Plus system includes 200 nm, 214 nm, 230 nm, and 254 nm filters by default.
The following section is for reference, if a different filter is required for your application.
Required Materials
• Filters
• Latex gloves
• Small forceps
1 Using the Direct Control window, move the trays to the load position.
2 Lift the outer door and then the cartridge cover door. The cartridge cover door is to the left of
the UV detector as shown in Figure 1.1.
3 Loosen the two thumbscrews and lift the clamp bar.
4 Remove the capillary cartridge from the interface block.
5 Turn the system power off.
6 Loosen the two thumbscrews and remove the optics source assembly. Put the assembly on a
clean work surface. The optics source assembly is located right behind the interface block and
has a horizontal bar handle as shown in Figure 1.3.
7 Remove the filter wheel access cover and rotate the filter wheel until the correct filter is
visible (Figure 1.3).

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Figure 1.3 Remove the Filter Wheel Access Cover
8 Put on latex gloves and use small forceps to remove the filters from the filter wheel. Touching
the optical surfaces of the filters without gloves leaves skin oil that can cause improper filter
performance.
9 Inspect each filter for a burned spot. Replace any filters that appear damaged.
The filters are affected by heat and time. It is good practice to periodically check filter
performance with a spectrometer or to replace the filters.
10 Insert the new filter with the reflective side facing inward (Figure 1.4).
1. Open cover at the indicated point
2. Access Cover
3. Thumbscrews (2), one on each side