Slee MICROTOME CUT 4062 User manual

DESIGN &
MANUFACTURING
MADE IN GERMANY
O P E R A T I N G
M A N U A L
MICROTOME
CUT 4062
MANUAL
ROTARY MICROTOME

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CONTENTS
1INTENDED USE _______________________________________________________ 5
2SYMBOLS____________________________________________________________ 5
3SAFETY NOTES _______________________________________________________ 5
3.1 HAND WHEEL STOP 6
3.2 FINGER PROTECTION GUARD 8
3.3 WORKING WITH KNIVES AND DISPOSABLE BLADES 9
4COMPONENTS ______________________________________________________ 10
4.1 MAGNIFIER WITH ILLUMINATION, DIMMABLE (OPTIONAL) 11
5SPECIFICATIONS_____________________________________________________ 14
6UNPACKING AND INSTALLATION _____________________________________ 15
6.1 UNPACKING THE DEVICE 15
6.2 INSTALLATION 15
7OPERATION OF CUT 4062 ____________________________________________ 16
7.1 INSERTION OF SPECIMEN IN STANDARD SPECIMEN CLAMP 16
7.2 INSERTION OF SPECIMEN IN UNIVERSAL CASSETTE CLAMP 17
7.3 SPECIMEN ORIENTATION ADJUSTMENT 18
7.4 EXCHANGING THE CLAMP TYPE 19
7.5 INSERTION AND ORIENTATION OF MICROTOME KNIVES 24
7.6 INSERTION AND ORIENTATION OF DISPOSABLE BLADES 25
7.7 APPROACH TO SPECIMEN 30
7.8 COARSE ADVANCE 31
7.9 MANUAL CUTTING 32
8CLEANING AND MAINTENANCE ______________________________________ 33
8.1 CLEANING 33
8.2 BATTERY REPLACEMENT 33
8.3 RECOMMENDED MAINTENANCE AND SERVICE SCHEDULE 34
9SERVICE ____________________________________________________________ 35

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10 OPTIONAL ACCESSORIES_____________________________________________ 36
11 WARRANTY_________________________________________________________ 37
12 DISPOSAL __________________________________________________________ 37

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1INTENDED USE
The rotary microtome CUT 4062 is intended for cutting thin sections of soft paraffin-embedded and harder specimens for
professional use in routine and research laboratories in the fields of biology, medicine and industry.
The system of specimen advance operates very reliable from 0.5 to 60 µm. The quality of cutting of this microtome is
increased by the automatic retraction during the upstroke of the specimen, which avoids rubbing on the disposable blades
or microtome knives. This stops rapid deterioration of the disposable blade or microtome knife.
2SYMBOLS
Slee rotary microtomes are provided with the following safety features:
CUT 4062
Hand wheel stop •
Finger protection guard for knife- or blade-holder •
The institution which owns the unit and the persons working with the unit, servicing or repairing it have the responsibility
for a hazard-free use.
Dangers, warnings and cautions are marked by this symbol.
This symbol indicates possible fire hazards.
iSpecial instructions regarding the operation of the device are marked by this symbol.
Mechanical components that can lead to injuries during operation are marked with this symbol.

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MAGNIFIER WITH LIGHTING (OPTIONAL)
3.1 HAND WHEEL STOP
3.1.1 HAND WHEEL STOP WITH BRAKE ON THE HANDLE
Always use the finger protection guard with the knife- /
blade holder and put hand wheel in stop position
•before working with the knife or specimen,
•before changing the specimen,
•during break time.
The hand wheel can be stopped in any position. For this
purpose, move stop towards the center. To do this, turn
the locking lever 90° counterclockwise.
To release the handwheel lock again, turn the lever 90°
clockwise.
Risk of glare and injury! Never look into the sun or any other bright light source with optical
devices! Do not look directly into the light source.
Fire hazard! Lenses in optical devices can cause considerable damage due to the "burning glass
effect" if improperly handled or stored! Make sure that optical lenses are never left in the sun
without a cover!
Use the supplied cover!

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3.1.2 HANDWHEEL STOP WITH BRAKE ON THE BASE (OPTIONAL)
Always use the finger protection guard with the knife- /
blade holder and put hand wheel in stop position
•before working with the knife or specimen,
•before changing the specimen,
•during break time.
The basic brake can be activated in every handwheel posi-
tion. To close, turn the lever for 90° clockwise vertically
(notch).
Basic brake open
To release the handwheel lock, turn the lever 90° counter-
clockwise.
Basic brake closed

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3.2 FINGER PROTECTION GUARD
Use the finger protection guard always
•before you start any work with the knife or spec-
imen,
•before changing the specimen,
•during work break.
Finger protection guard released
Finger protection guard activated
i
Please note that the finger protection guard may be engaged throughout processing (exchange
of blade, lateral displacement, trimming, cutting), thereby increasing operation safety!
Do never move your fingers or hands above or behind the blade holder during operation of the
microtome. For loading and unloading of the sample holders and exchange of disposable blades
in the disposable blade holder, always activate the handwheel lock and activate the finger pro-
tection guard.

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3.3 WORKING WITH KNIVES AND DISPOSABLE BLADES
Microtome knives and disposable blades have extremely sharp edges and this can lead to injuries. Please be extremely careful
when handling microtome knives and disposable blades.
Do not place microtome knives or disposable blades at unsecured areas. Never position micro-
tome knives or disposable blades with the sharp edge pointing upwards.
Store blades in a covered container. Use a container that has guides to hold the blades rigid.
Never try to catch a falling microtome knife.
Always insert the specimen first and then the microtome knife or disposable blade.
When applying the brake, ensure that it is tight. Most accidents occur when the brake slips and
the operator’s hand is drawn into the blade.
To avoid compression or knife marks, ensure that the blade is clean.
In case of multiple use of the knife or blade, use the finger guard.
To increase the usage time of knives and blades, avoid increased wear and tear by cleaning the
cutting edge and blade holder regularly.

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4COMPONENTS
Slee rotary microtomes are provided with the following standard components:
CUT 4062, #10065000
Specimen orientation •
Universal cassette clamp, orientable, alternatively standard specimen clamp, orientable •
3-component knife holder, can be used for low profile blades as well as high profile blades •
Section waste tray •
Disposable blades (low profile, 50 pcs.), optional with disposable blades (high profile, 50
pcs.) or microtome knife 16 cm C-profile
•
Dust cover •
Allen key, for fixation of specimen orientation to microtome •
Manual •

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4.1 MAGNIFIER WITH ILLUMINATION, DIMMABLE (OPTIONAL)
Mounting:
The ring magnifier with illumination can easily be at-
tached to the side of the microtome's base plate.
2 M5x12 countersunk screws (supplied) are screwed into
the base plate from below.

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Switching on / off:
The light can be switched on and off via the sensor switch
on the light head.
Switching on: Touch the sensor for a brief moment -> the
light will be switched on (switch-on value is the last dim-
ming value).
Switching off: Touch the sensor again for a brief moment
-> the light will be switched off.
Dimming: Touch the sensor until the desired brightness is
obtained. To reverse the dimming direction, briefly inter-
rupt touching the sensor.
Note:
The illumination is equipped with overheating protection. In case the luminaire is switched off because of a risk of overheat-
ing, the luminaire must be cooled down, before being put back into operation.
Warning: Fire hazard!
When not in use, cover the magnifier against solar radiation. To do this, use the bag in-
cluded in the scope of delivery.

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Specifications magnifier with illumination:
Protection class II
Protection type IP 20
Operating mode Continuous operation
Technical safety check EN 60 598-1
Light head ca. Ø 122 x 13 mm
Magnifier 6.0 dpt. approx. Ø 72
Rated voltage 100 - 240 V AC
Frequency range 50/60 Hz
Power consumption approx. 6 W

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5SPECIFICATIONS
Operating temperature range +10 to +35 °C
Operating humidity max. rel. 80 % non-condensing
Storage temperature range +5 to +55 °C
Storage humidity max. rel. 80 % non-condensing
Section thickness range 0.5 - 60 µm
Section thickness settings 0.5 - 2 µm in 0.5-µm steps
2 - 10 µm in 1-µm steps
10 - 60 µm in 2-µm steps
Trimming thickness settings 10 µm, 20 µm, 30 µm, 40 µm
Horizontal advance 28 mm (via coarse feed handwheel)
Vertical stroke 60 mm
Maximum specimen size 50 x 50 mm
Knife holder basis - north-south axis 35 mm
Specimen retraction on return stroke automatic
Specimen orientation, horizontal 8°
Specimen orientation, vertical 8°
Specimen orientation horizontal / vertical optionally clockwise or counter clockwise (Standard:
When turning
counter clockwise, the sledge moves out.)
Specimen orientation, z-axis 360°
Section counter strokes (optional)
Dimensions [width x depth x height] 560 mm x 550 mm x 325 mm incl. handwheel and waste tray
Weight without accessories 33 kg

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6UNPACKING AND INSTALLATION
6.1 UNPACKING THE DEVICE
Remove the upper wooden cover.
Remove the upper supporting foams.
Lift the device out of the wooden transportation case.
Grasp the device underneath the base plate from the rear and front as shown in the figure below.
Save the packaging material for later transport purposes, as the microtome should be transported in its original packaging
to avoid transport damage.
Set up the microtome at its destination.
6.2 INSTALLATION
The unit should be positioned onto a plane, vibration-free surface.
Assure convenient and unobstructed access to the hand wheel.
Before use remove the transport security underneath the specimen head.
i
Do not transport the device by holding it on the hand wheel shaft, the object head, the cover or
the tray.

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7OPERATION OF CUT 4062
7.1 INSERTION OF SPECIMEN IN STANDARD SPECIMEN CLAMP
Turn the handwheel to the highest position and activate the
handwheel lock (see chapter 3.1) and the finger guard.
Loosen the standard specimen clamp by turning the fixing
screw counterclockwise.
Remove the old specimen or insert a new one.
Loosening of object fixation and insertion of new object
Close the specimen clamp and fix the specimen by turning
the clamping screw (see fig.) clockwise.
Loosen the finger guard and the handwheel lock (see chap-
ter 3.1) to cut the specimen.
Closing of specimen clamp and fastening of object fixation
If the cassette is clamped horizontally (rotated 90°), it is rec-
ommended to use the supplied clamping block (item no.
34123507) to re-center the cassette. If the cassette is nor-
mally clamped vertically, this additional clamping block is
not used.
In order to bring the specimens closer to the middle section,
the clamping block must be clamped to the upper cheek.
Clamping block
Additional note:
In case you process exceptionally hard specimens, the required clamping force may not be achieved by simply tightening the
clamping screw. For these exceptional cases, an attachment for an open-end wrench SW17 is provided in the middle area
of the clamping screw. We would like to point out that very high clamping forces can occur when using tools and this can
lead to increased wear on the corresponding components.

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7.2 INSERTION OF SPECIMEN IN UNIVERSAL CASSETTE CLAMP
Turn hand wheel to its highest position and activate the
hand wheel lock.
Activate finger protection guard.
Open cassette fixation by pulling the fixation lever.
Insert / remove cassette.
Object fixation will go back into fixation position automati-
cally.
Release finger protection guard and hand wheel lock (see
chapter 3.1) for cutting.
Opening of cassette fixation and insertion of new cassette

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7.3 SPECIMEN ORIENTATION ADJUSTMENT
Turn hand wheel to its highest position and activate the
hand wheel stop (see chapter 3.1).
For orientation of the specimen, open the fixing lever on
the right side of the orientation.
For upward and downward orientation use the upper ori-
entation wheel (see fig.).
For orientation to the left and right use the left orientation
wheel (see fig.).
Opening of orientation fixation lever and orientation of
specimen holder upwards and downwards
For orientation in the z-axis, turn the cassette clamp (see
fig.).
Fix the specimen orientation by locking the clamping lever
on the right side of the specimen holder.
Loosen the finger guard and the handwheel lock (see chap-
ter 3.1) for cutting the specimen.
Change specimen orientation to right or left and sample
orientation in z-axis

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7.4 EXCHANGING THE CLAMP TYPE
For exchanging the universal cassette clamp with specimen orientation with the standard specimen clamp (fixed), please
proceed as follows:
Move the universal cassette clamp to the upper position
and fix the hand wheel by pressing the brake lever.
Pull out the right handle of the specimen orientation to re-
lease the universal cassette clamp out of the system.
Use a 6 mm Allen wrench to remove the screw out of the
center of the specimen orientation.

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Disassemble the specimen orientation.
Disassemble the standard specimen clamp to get access to
the center screw – M8 x 50 mm.
Install the basic part of the standard specimen clamp and
fix it with the Allen screw.