Slee MICROTOME Cuttec S User manual

DESIGN &
MANUFACTURING
MADE IN GERMANY
MICROTOME
Cuttec S
MANUAL
SLIDING MICROTOME
O P E R A T I N G
M A N U A L

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CONTENTS
1INTENDED USE _______________________________________________________ 4
2SYMBOLS____________________________________________________________ 4
3SAFETY NOTES _______________________________________________________ 4
4COMPONENTS _______________________________________________________ 6
5SPECIFICATIONS______________________________________________________ 6
6UNPACKING / SETTING UP THE DEVICE _________________________________ 7
7FUNCTIONS OF THE DEVICE ___________________________________________ 7
8LIST OF REFERENCE NUMBERS _________________________________________ 8
9OPERATION_________________________________________________________ 10
9.1 WORKING WITH KNIVES AND DISPOSABLE BLADES 10
9.2 PREPARATION OF A CUT PREPARATION 11
9.3 ACCEPTANCE OF THE CUT PREPARATION 11
10 ERRORS AND SOLUTIONS ____________________________________________ 12
11 CLEANING AND MAINTENANCE ______________________________________ 13
11.1 CLEANING 13
11.2 RECOMMENDED MAINTENANCE AND SERVICE INTERVALS 14
12 SERVICE ____________________________________________________________ 14
13 OPTIONAL ACCESSORIES_____________________________________________ 15
13.1 STANDARD ACCESSORIES 15
14 WARRANTY_________________________________________________________ 16
15 DISPOSAL __________________________________________________________ 16

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1INTENDED USE
The sledge microtome Cuttec S is a laboratory device and is used for making thin sections of specimens of varying
hardness and texture. The device is intended exclusively for professional routine and research laboratory applica-
tions in biology, medicine and industry.
The system reliably cuts samples in the cutting range of 0.5 to 60 μm.
2SYMBOLS
Slee sledge microtomes are provided with the following safety features:
Cuttec S
Finger protection for disposable- and standard knife holder •
Hazards, warnings and preventive measures are marked with this symbol.
iAdditional application information and useful tips are marked with this symbol.
Mechanical components which can lead to injuries during operation of the device are marked
with this symbol.

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In order to ensure the performance of the device and ensure safe operation, the user must ob-
serve the instructions, recommendations and warnings.
The operating instructions must be read in full before use. The operating personnel must be
trained according to the operating instructions.
The device must be placed in a fixed and stable place. The protective devices must not be dis-
mantled.
Before mounting the accessories or a preparation, the carriage must always be locked in the rear
position with the fixing.
No blade / blade holder may be clamped when inserting or changing the object clip. When in-
serting an object into the object clip, the finger guard must be pushed in front of the knife /
disposable blade.
The operating personnel must be trained according to the operating instructions.
Always wear protective goggles when working on hard specimens.
When cleaning the appliance, the blade / disposable blade holder must always be removed. No
solvents such as acetone may be used. No cleaning agent may enter the appliance.
When transporting, the carriage must be locked in the middle position.
Do not lift by moving parts. Only lift the device by its base.
Maintenance work may only be carried out by the trained service technician.
Despite existing safety constructions there is a residual risk at all times when dealing with the
product and the necessary accessories. Cut wounds are to be expected, especially when handling
a microtome knife.

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4COMPONENTS
Slee sledge microtomes are delivered with the following standard components:
Cuttec S, #10040001
Blade rail (for low profile disposable blades) •
Universal cassette clamp
optional with standard-object-clamp
•
Standard knife holder
optional with disposable blade holder
•
Section waste tray •
User manual •
Dust cover •
Disposable blades (low, 50 pcs.)
Optional with disposable blades high or microtome knife 16 cm C-profile
•
Tool set •
5SPECIFICATIONS
General Information
Operating temperature range
+10 to +35 °C
Operating humidity
max. rel. 80 % non-condensing
Storage temperature range
+5 to +55 °C
Storage humidity
max. rel. 80 % non-condensing
Cutting range
0 - 60 μm
Cutting thickness settings 0 - 15 μm in 1-μm steps
15 - 60 μm in 5-μm steps
Automatic delivery
0 – 60 μm
Sample delivery
30 mm
Maximum sample size
50 x 70 x 20 mm
Sample orientation
+/- 8°
Cutting distance
180 mm
Dimensions (length x width x height)
450 x 150 x 200 mm

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6UNPACKING / SETTING UP THE DEVICE
Before unpacking, check the pack for possible damage. If damage is noticed, inform your carrier or supplier.
Open the cardboard box and remove the protective material and small parts.
Remove manual and small parts.
The carriage must remain fixed for transport.
Lift the device only by the base of the unit.
The device must be placed in a fixed place with sufficient working space.
Remove the transport protection.
7FUNCTIONS OF THE DEVICE
•Horizontal moving sledge / knife holder (cutting motion)
•Locking via brake lever
•Locking automatically
•Process object via coarse feed wheel
•Cutting thickness adjustment
•Feeding movement manually
•Feeding movement automatically
•Object holder clamp
•Object holder orientation
•Knife clamping
•Angle of bevel (for solid section)
•Free angle clamping
•Free angle orientation

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8LIST OF REFERENCE NUMBERS
1
Disposable blade holder
2
Finger protection
3
Basic body
4
Section waste tray
5
Clamp lever object clamp
6
Cylinder
7
Object orientation
8
Feed lever
9
Coarse wheel
10
μm-setting
11
Object clamping
12
Universal cassette clamp
13
Clamping screw for disposable blade holder / knife holder
14
Clamping lever disposable blade holder / knife holder
15
Free angle adjustment
16
Handle for slide movement
17
Adjustment screw automatic infeed
18
Brake

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1
2
3
5
4
6
7
8
9
10
11
12
13
14
17
15
16
18

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9OPERATION
1. Use the universal clamp or the standard clamp
2. Insert the sample
3. Align the sample
4. Clamp the sample
5. Adjust the oblique position of the knife holder
6. Adjust the cutting angles
7. Insert the blade holder or knife
8. Tighten the blade / blade holder
9. Adjust the thickness
10. Push the finger protection aside
11. Release the brake on the slide
12. Run the sample with the coarse wheel to the cutting plane
13. Cut the sample
14. Continuous cutting
9.1 WORKING WITH KNIVES AND DISPOSABLE BLADES
Microtome knives and disposable blades have extremely sharp edges and this can lead to injuries. Please be
extremely careful when handling microtome knives and disposable blades.
Do not place microtome knives or disposable blades at unsecured areas. Never position micro-
tome knives or disposable blades with the sharp edge pointing upwards.
Store blades in a covered container. Use a container that has guides to hold the blades rigid.
Never try to catch a falling microtome knife.
Always insert the specimen first and then the microtome knife or disposable blade.
In case of multiple use of the knife or blade, use the finger guard.
To increase the usage time of knives and blades, avoid increased wear and tear by cleaning the
cutting edge and blade holder regularly.

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9.2 PREPARATION OF A CUT PREPARATION
Align the specimen clamped in an object clamp in the horizontal section with the object orientation. Fix the
object clip with the clamping lever. Move the blade over the object by moving the carriage. Use the coarse wheel
to move the object slowly in the measuring plane. Before first touching the object with the knife, move the
preparation upwards using only the infeed lever or the automatic infeed. Do not set the cut thickness above
30 μm. The infeed must only be carried out when the blade is brought into the rear position with the carriage.
After each delivery procedure, pull the knife with the carriage forward over the preparation. As soon as the first
contact of the blade with the specimen has taken place, set the required cutting thickness and repeat the process
until the desired cutting plane is reached.
9.3 ACCEPTANCE OF THE CUT PREPARATION
Pull the sled to the front of the user. In the front end position the carriage is held automatically by spring force.
Now the cut can be taken with the help of brushes and wooden sticks.
Caution When the blade is in contact with the tool, the cutting edge can be damaged!
Be careful when handling the blade, risk of cutting.
i After the cut has been cut, always slide the carriage into the rear end position and slide the finger
protector in front of the cutting edge.

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10 ERRORS AND SOLUTIONS
Error Reason Solution
Excessive cutting Free angle set incorrectly Adjust the free angle
Excessive cutting Temperature of preparation too high Cool the preparation
Thick / thin section Cutting blunt Replace the blade
Thick / thin section Knife not tightly clamped Check the blade clamping
Thick / thin section Preparation not tightly clamped Check the clamping of the specimen
Chatter marks in section Preparation too hard Use a knife for harder preparations
Chatter marks in cut Preparation is not fixed in the object clip Check the paraffin supernatants on the
cassette and scrape them if necessary.
i
Please note that you can activate the finger protection during the entire working process (blade
change, lateral displacement, trimming, cutting) and thus considerably increase the working
safety!
Do not hold your fingers or hands over or behind the blade or knife holder during operation.
To secure or remove the sample and replace the disposable blades or knives, always activate
the handwheel lock and the finger guard on the blade or knife holder.

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11 CLEANING AND MAINTENANCE
11.1 CLEANING
The frequency of cleaning the microtome depends on the frequency of use and duration.
Wear gloves and protective clothing in accordance with good laboratory practice.
Please observe the safety instructions.
Use only aqueous detergents, no acetone or xylene.
The housing parts should be cleaned with commercially available plastic cleaners. Please do not use any alcoholic
or organic cleaning agents to clean the case.
Do not spray any cleaning agents directly on the operating films or the display.
Before cleaning the appliance, the blade / disposable blade holder must always be removed.
Before cleaning the device, move the sample holder downwards. Use the brush to free the device
from cutting residues. Use commercially available household cleaners on cloth cloths for wiping.
Do not spray appliance with detergent. Do not immerse the appliance in water. No liquid must
penetrate the interior of the appliance.
For disinfection, the device can be rubbed with a surface disinfectant on a cloth. Metallic surfaces
can be cleaned with solvent (e. g. acetone) on a cloth. Cleaning is recommended after each
operation. To maintain the cylinder guide, it should be lubricated after the cleaning process.
The device may only be opened by a trained service technician for maintenance work. To do this,
please contact the sales company you purchased the device from.

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11.2 RECOMMENDED MAINTENANCE AND SERVICE INTERVALS
Daily Remove section waste from the waste tray.
Please use supplied dust cover when device is not in use.
Weekly Cleaning of blade holder.
Yearly Annual inspection (performed by authorized Slee service technician)
•Check of all functions
•Lubricating of movable parts
•Check of driving system
•Complete cleaning
12 SERVICE
Internal components may only be serviced by authorized technicians.
If technical service or spare parts are necessary, please contact your local SLEE medical GmbH distributor. Please
have the following information available:
•complete contact details,
•type of device and serial number,
•location of device and name of user,
•purpose of service call,
•delivery date of the unit.
If it is necessary to return the device, it must be cleaned and disinfected before delivery. It must be returned in its original
packing.
If the device or parts thereof are sent back in a dirty or non-disinfected condition, SLEE medical GmbH reserves the right to
return the parts to the debit of the customer.

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13 OPTIONAL ACCESSORIES
13.1 STANDARD ACCESSORIES
Description Item No. Cuttec S
Universal cassette clamp, orientable 10090032 •
Standard object clamp, orientable 10090033 •
Dispoable blades (low; 50 pcs.) 28407000 •
Dispoable blades (high; 50 pcs.) 28408000 •
Adhesive foil for pickup of microtome sections 10090029 •
Dissection needle 30030095 •
Microtome knife profile C, length 10 cm 28000002 •
Microtome knife profile C, length 12 cm 28000010 •
Microtome knife profile C, length 16 cm 28000026 •
Microtome knife profile C, length 18 cm 28000034 •
Microtome knife profile C, length 30 cm 28000058 •
Microtome knife profile C, length 16 cm 28000027 •
Microtome knife other versions
profile C or D, length 10 to 40 cm
on
demand

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14 WARRANTY
SLEE medical GmbH guarantees that the product delivered has been subjected to a comprehensive quality control procedure,
and that the product is faultless and complies with all technical specifications and / or agreed characteristics warrant-ed.
SLEE medical GmbH guarantees that the device is manufactured under an ISO 9001:2015 and ISO 13485:2016 quality
management system.
Unauthorized modification or repair by third party persons will void the warranty.
Only original SLEE medical GmbH spare parts must be used.
Guarantee claims can be put forward only if the device is used according to this manual and for the purpose described.
Mistakes and errors which occur because of improper use cannot be accepted.
15 DISPOSAL
The device or parts of the device must be disposed of according to existing local applicable regulations.

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Notes

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Notes

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Notes

LEE medical GmbH • Am Neuberg 14
55268 Nieder-Olm • Germany
www.slee.de
T: [phone]
E: [email protected]
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