Sper scientific 300001 User manual

Sugar/Brix
Refractometer
0 - 32%
300001
28 - 62%
300002
Instruction Manual

2
Prism
Calibration screw
Cover Plate
Barrel
Eyepiece with focus ring
INTRODUCTION
Your portable refractometer is a precision optical instrument
designed to measure the concentration of sugar in aqueous
solutions. It utilizes a Brix scale that is accurate and easy
to read. It is excellent for quality assurance, process control
requirements, and research. Refractometers operate on the
principle that as the concentration or density of a solution
increases, its refractive index changes proportionately. The
refractive angle measured by your refractometer registers on
the scale. The larger the concentration of sugar in solution,
the higher the reading on the scale.
DESCRIPTION
CONTENTS
INTRODUCTION ............................................................ 2
DESCRIPTION................................................................ 2
OPERATING PROCEDURES........................................ 3
PRECAUTIONS .............................................................. 4
SPECIFICATIONS .......................................................... 4
WARRANTY .................................................................... 4

2 3
OPERATING PROCEDURE
1. With the COVER PLATE open, clean the PRISM with a
soft cloth to avoid scratching the surface.
2. Aim the refractometer toward a light source and rotate
the EYEPIECE to obtain the clearest focus.
3. Zero Point Calibration:
A. Open the COVER PLATE.
B. Apply a few drops of the included calibration solution
onto the PRISM platform.
C. Close the COVER PLATE.
D. Turn the CALIBRATION SCREW until the dark and
light boundary line coincides with the bottom line
of the scale. The bottom line is 0 for 300001 Model
and 28 for 300002 Model.
4. Carefully dry the prism platform and COVER PLATE.
5. Place a few drops of the test solution on the prism and
close the COVER PLATE so the solution spreads evenly
on the prism.
6. Aim the front of the refractometer towards the light
source and focus the eyepiece on the boundary line.
7. The boundary line indicates the concentration of sugar in
the test sample at 20°C.
8. After use, clean the prism with distilled water and a cloth
to remove any residue.
9. The temperature of the zero point calibration should
be at the same temperature as the test solution. For
variations in temperature the zero point should be
adjusted once every 30 minutes.
15% Sample (300001)
20°CBrix%
0
5
10
15
20
25
30
0
5
10
15
20
25
30
45% Sample (300002)
20°CBrix%

4
PRECAUTIONS
Never submerge the unit, and do not let liquid seep into the
unit’s body. Clean the refractometer after each use with a soft
cotton cloth. Do not scratch surface of the prism. Store in a dry,
clean, and non-corrosive environment. Avoid strong impacts.
IMPORTANT NOTE...
This Sugar/Brix Refractometer is intended for solutions
containing sugar solute. Any test solutions that contain salt
will a󰀨ect the reading.
SPECIFICATIONS
Model 300001 300002
Range 0-32% 28-62%
Resolution 0.2%
Accuracy ±0.2%
Size 6 3/4” x 1 1/2"
(171 x 38 mm)
6 1/4” x 1 1/2”
(159 x 38 mm)
Weight 3.1 oz. (89 gr.) 3.2 oz. (90 gr.)
Calibration Solution Distilled Water 28% Brix
Accessories Screwdriver, Carrying Case, Transfer Pipette, Manual.
WARRANTY
Sper Scientic warrants this product against defects in
materials and workmanship for a period of ve (5) year from
the date of purchase, and agrees to repair or replace any
defective unit without charge. If your model has since been
discontinued, an equivalent Sper Scientic product will be
substituted if available. This warranty does not cover probes,
batteries, battery leakage, or damage resulting from accident,
tampering, misuse, or abuse of the product. Opening the
meter to expose its electronics will break the waterproof seal
and void the warranty. To obtain warranty service, ship the
unit postage prepaid to:
SPER SCIENTIFIC LTD.
8281 E. Evans Rd., Suite #103
Scottsdale, AZ 85260
[phone]
The defective unit must be accompanied by a description of
the problem and your return address. Register your product
online at www.sperwarranty.com within 10 days of purchase.
Rev 12/6/2017