STEINDORFF NYMCS-290 User manual

STEINDORFF®
Inverted Biological
Microscope
NYMCS-290
User Manual
This manual is written for Inverted Biological Microscope NYMCS-290. For safety and for exerting the best
performance, making you familiar with the instrument entirely, it is strongly recommended that you read this
manual carefully before using the microscope. For the further reference, please place this manual in a position
where nearby the worktable and fetched easily.

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STEINDORFF®
1. Do not keep the instrument in a direct sunlight, high
temperature or humidity, dusty and easy shaking
environment. Make sure the stage is plane, horizontal and
stable enough.
2. When moving the microscope, please hold up the
instrument with one hand on the lower side of the eyepiece
tube ①, and the other hand on the illumination bracket
②. (Figure 1)
3. If the bacterium solution or the water splash to the stage,
objective or viewing tube, pull out the power cord at once,
and wipe up the microscope. Otherwise, the instrument
will be damaged.
4. When working, the lamp house on the top of the arm ③
(Figure1) will become very hot, be sure there have enough
room around the lamp house (especially the top) for cool.
5. Before replacing the lamp bulb or fuse, turn the main
switch on the “O” (off) position, then cut off the power. If
the lamp is on, or soon after it has been turned off, it is hot
and will cause serious burns, please do the replacement
after it cool down completely.
Specified: the halogen lamp 6V30W
6. Earth this instrument to prevent the lightning strike.
7. Use the supplied power cord, please.
Safety Note
Maintenance
oUse the gauze to wipe the glass parts gently .If removing the fingerprints and oil stains, slightly dampen
gauze with the xylene or the admixture liquid which comparison is 3:7 of the ethanol and the ether to
wipe.
 Note: the ethanol and the ether are all very combustible, do not put these chemicals near fire or
the possible electricity spark source such as the electronic equipment open and close operation.
Use these chemicals in a well ventilated room as far as possible.
oDon’t use organic solvent to wipe the non-optical elements, If you need to clean, use the neutral
detergent, please.
oWhen using, if the microscope is splashed by liquid, cut off the power at once, and wipe up the
moisture.
USER NOTICE
Figure 1
Figure 2

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oDo not disassemble any parts of the microscope. That will affect the function or decline the
performance of the microscope.
oIf haven’t mounted the objective, please cover the dust cap to prevent the dust and the splashed liquid of
the tissue culture entering the inside.
oWhen not using, remember to cover up the microscope with the dust casing. And make sure the lamp
cools down enough before you do so.
Safety Symbol
Symbol
Meaning
The surface is very hot, not touch by your hands
Before using, please read the instruction carefully, improper operation will result in bodily injure or
instruction malfunction.
The main switch on
The main switch off

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STEINDORFF®
1. COMPONENT NAMES
Objectives
4x
10x (PH)
20x (PH)
40x
Phase contrast slider
Lamp house
Illumination bracket
Condenser
Stage inserted plate
Ocular (eyepiece)
Trinocular viewing
tube (Fixed)
Microscope base
Stage (Fixed)
Objective turret (Nosepiece)
(Fixed)
Figure 1

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2.1. Installing diagram
The following figure shows the installation sequence of the components.
 Before installing, be sure every components is clean, do not score any parts or glass surface.
 Keep well with the supplied hexagon wrench. When changing the components, you will need it
again.
2. INSTALLATION
Figure 2
Color Filter
Lamp house
Condenser
illumination unit
Filter holder
Ocular (eyepiece)
Glass stage
Objectives
Power cord
Mechanical ruler
Stage lengthen splint
Microscope base

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2.2.1. Installing and replacing the lamp (Figure 3)
Please use the specified halogen Lamp 6V30W
1. Hold to the bulb ①after you wrap it with gauze or other
protection materials, then depress the plugs ②into the
jack ③on the lamp house, ensure the filament and the bolt
④are in a same level.
2. Replacing the lamp when using or soon after.
When using, or soon after it is turned off, the lamp, the
lamp house and nearby parts will be very hot and will
cause serious burns. Please turn the main switch on “O”
(off), pull up power plug, and make sure the bulb, the lamp
house and periphery are all cool. Then, you can do your
replacing.
Please insert the lamp gently, or it will be damaged by
excessive extrusion.
Do not touch the Halogen bulb with your hands. It will
shorten the service life or cause it to burst. If you leave
fingerprints on the surface carelessly, clean it with a dry
soft cloth.
2.2.2. Installing the condenser illumination unit (Figure 4)
1. Insert the condenser illumination unit ①into the bracket
②gently, according the figure showed in the left.
2. Turn the condenser illumination unit at clockwise about
90°, let the “AS” mark of filter holder ③facing forwards,
and keep the screw of condenser illumination unit and the
hole of the holder in line, then screw down the bolt in the
hole with the supplied hexagon spanner.
3. Insert aviatic BNC connector plugs ④into aviatic BNC
connector jack ⑤.
2.2.3. Installing the lamp house (Figure 5)
Keep the BNC connector plugs ①and the lamp house pin ②in line, and keep the bolt ③and the condenser jack ④
in line, too. Then push the lamp house into the illumination unit gently until they are against.
2.2. Installing steps
Figure 3
Figure 4
Figure 5

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2.2.4. Installing the objective (Figure 6 and 7)
1. Turning the coarse fusing knob ①like the figure shows till
the nosepiece get to its lowest position.
For ensuring the safety of the instruction on
transportation, the nosepiece is located in the lowest
position and the tension adjustment collar ②is adjusted
in an appropriate tight tension while leaving the factory.
2. Screw the lowest magnification objective on to the turret
from the nearside, then turn the turret clockwise, mount
other objectives according the magnification sequence of
low to high.
oMount objective like this way will make the change of
magnification to be very easy in using.
oIt also can install the objective through the stage opening.
Clean the objective regularly, the objective used in the
inversed microscope is very sensitivity about dust.
Do cover all the unused holes with turret dust caps ③, to
prevent the dust and contamination entering inside.
When operating, use the low magnification objective (4X or
10X) to search and focus the specimen at first, then replace
the higher magnifications if necessary.
When replace the objective, slowly turning the nosepiece
until you hear “clicked”, which means the objective enter
into the right position - center 7 of the light path.
Figure 6
Figure 7
Figure 8

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2.2.5. Installing the stage lengthen splint and the mechanical ruler (Figure 8)
oStage lengthen splint can be installed in either side of the stage to enlarge the work surface. But you can’t install the
mechanical ruler together.
oGenerally, the mechanical ruler will be installed in the right side for comfortable adjustment.
1. Installing the stage lengthen splint
First, Screw the fixed bolt①on to the splint , then mount it on to the stage from right or left below, screwing down it
until it stay hard.
2. Installing the mechanical ruler
Please install the ruler like the way of the stage splint.
2.2.6. Installing the stage inserted plate (Figure 9)
1. When using the glass stage ①, there is no special require, you just need to place it in a plane.
2. Install the stage inserted plate on to the stage opening.
oTurn the disk, let the V nick to face user, so the recognition of the objective will become easier.
2.2.7. Installing the eyepiece (Figure 10)
1. Remove the cap of the eyepiece tube ①.
2. Insert the eyepiece into tube until they are against.
2.2.8. Installing the color filters (Figure 11)
Be sure the color filter cools down completely before you change them. Take down the filter holder ①, then
install the color filters ②you need.
oMount the color filter downwards like ③shown, keep it horizontal through the end, not allow inclined.
If the color filter is inclined or not get to the end, it will drop possibly.
oThe color filter could be piled on the holder, so you can install more than one filter according the needs if you can
ensure the whole thickness is less than 11mm.
Figure 9
Figure 10
Figure 11

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2.2.9. Connecting the power cord (Figure 12, 13 and 14)
Do not bring the power cord to bear a powerful stress.
When being bent or wrapping, the cable and wires will be
broken easily.
1. Turn the main switch ①on “O” (off) state before
connecting the power cord.
2. Insert the plugs ②into the power jack ③of the
microscope safely.
3. Plug the power cord ④into the power supply receptacle.
Make sure the connection is well.
4. Insert aviatic BNC connector plugs ⑨into aviatic BNC
connector jack ⑧
Do use the supplied power cord all the time. If lost or
damaged, select the same standard cord, please.
Connect the power cord correctly, to ensure the
instrument is earthing.
2.2.10. Replacing the fuse (Figure 12 and 13)
Do remember to turn the main switch ①on the state of “O” (off)
before replacing the fuse, and unplug the power cord. Rotate the
fuse ⑥kits out of the holder ⑦by the “--”type screwdriver,
replace a new fuse, then rotate back to the holder again.
Fuse rating: 250V，500mA.
Figure 12
Figure 13
Figure 14

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3.1.1. Turning on the lamp (Figure 15)
Connect the power, turn on the main switch ①which on the
bottom side of the base to “ - ”(on).
3.1.2. Adjusting the brightness (Figure 16)
Turning the brightness adjustment knob clockwise, the voltage
raise, and the brightness strengthen; whereas turning at the contra
direction, the voltage decline, and the brightness weaken.
oUsing the lamp in a low voltage condition, will prolong
the service life.
3.1.3. Adjusting the tension adjustment collar (Figure 17)
The tight tension of the coarse focus knob ②had
already adjusted before leaving factory.
oHow to adjust the tight tension
Turning the tension adjustment collar ①. While revolving
at the direction which shown by the arrowhead on the
figure, the tight tension of the coarse focus knob ②is
increasing; and if at the contra direction, the tight tension
will decline.
If the nosepiece dropped automatically, or the specimen
defocused soon even you focus with the fine focus knob
③. It means the coarse focus knob is too loose, you
should screw it down at the direction shown by the
arrowhead in the figure 17.
3.1. Microscope base
3. OPERATING THE ADJUSTMENT
Figure 15
Figure 16
Figure 17

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3.2.1. Setting the specimen (Figure 18 and 19)
Set the specimen in the center of the stage, please.
To obtain the best observe effect, please select the
containers, such as culture dish and culture bottle, with the
bottom thickness is 1.2mm, and the same thickness is also
required by the object slide when it is laid the specimen.
oUsing the Φ35mm culture dish
You can lay a Φ35mm culture dish on the stage directly by
using the standard center board ①of the stage.
oUsing the mechanical ruler
1. When using the 96bit or 24bit micro-titration board, please
fasten it tightly by the stage clips ②.
2. When fastening other model boards, please use the
following supplied brackets with mechanical ruler:
Terasaki bracket ③for Terasaki board
Culture dish bracket ④for Φ35mm culture dish
Object slide bracket ⑤for object slide and Φ54mm
culture dish
3. Turning the transverse knob ⑥and lengthways knob ⑦,
move the specimen to the required position. Movement
Range: 120mm (width) × 78mm (Length)
3.2. Stage
3.2.2. Moving the specimen
Turn the knob of the mechanical ruler or use your hands directly to move the specimen to the position you
wanted, please.
 Be careful when you replace the objective, please, especially after a short work distance
observation. Not let the objective to touch the stage inserted plate or the culture dish bracket.
Figure 18
Figure 19

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3.3.1. Adjusting the diopter (Figure 20)
1. Look into the right ocular by your right eye, then revolving
the coarse focus knob to focus on the specimen.
2. Then use your left eye to look into the left ocular. If the
image is not sharp, just use the diopter adjustment ring ①
to adjust please.
There are ±5 diopter in the adjustment ring. The
number which the reticle on the eyepiece holder pointed
is your eye’s diopter graduation.
3.3.2. Adjusting the interpupillar distance (Figure 21)
When observing with two eyes, hold on the left and right prism
holder, turn around the axis, adjusting the interpupillar distance until
the left and right fields of view coincide completely.
The reticle on the interpupillar distance indicator ③,
pointed by the spot “.” ②on the eyepiece holder, shows
the scale of the interpupillar distance. (Figure 20)
The range of the interpupillar distance：48～75mm.
3.3. The viewing tube
Figure 20
Figure 21
1

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3.3.3. Switching the light path (Figure 22)
oPulling out the light path selector lever ①by your thumb,
select the light path you needed.
oWhen in the binocular observation, pushing in the lever
until you heard a “clicked”. While in video or photography,
pulling out the lever until it reached the “clicked” position.
3.4.1. Using color filter (Figure 23)
oSelecting the appropriate color filters according your need,
it became more effective to observe or photography the
specimen. Especially, we suggest using the LBD color
filter, which can compensate more neutral colors.
oYou could pile up a group of color filters to the filter
holder, if you ensure they are level and the whole thickness
is less than 11mm.
Color Filter
Meaning
IF550
Single contrast color filter (green)
(used for the phase contrast microscopy)
LLBD
Color temperature transit color filter (blue)
(used for bright field observation and
microphotography)
Light path selector lever
Brightness proportion
Application
Pushing in the lever until it
reached the limit position
100% used for binocular
observation
Binocular observation
Pulling out the lever until it
reached the limit position
20% used for binocular
observation, and 80% used for
video or photography
Binocular observation, television,
and micrography or video can be
operated simultaneous
3.4. Illumination Unit
Figure 22
Figure 23

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3.4.2. Using the aperture diaphragm (Figure 24)
When in the bright field observation, the aperture
diaphragm control the numerical aperture of the
illumination system. Only when the numerical aperture of
the objective and the illumination system being matching,
you can obtain the higher image resolution and contrast, and
the increased depth of field, too.
To recognize the aperture diaphragm, you could remove the
eyepiece if necessary. (You also could insert in the center
telescope) then looked into the viewing tube, you might see
a field of view like the figure shown. The proportion could
be changed by dialing the aperture adjustment lever
according your need. (①is the image of the aperture
diaphragm, ②is the edge of the objective)
Generally, when observing the chromatic specimen, you
need to set the size of the condenser aperture diaphragm at
70%～80% of the numerical aperture which marked in the
objective. But if observing the bacterium specimen which
not colored, you could turn the aperture diaphragm lever at
the direction of “ ” (clockwise).
Figure 24

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4.1.1. Phase contrast objective (Figure 25)
oThe optional magnification of the phase contrast is: 10X,
20X.
oIf you want to know how to mount the phase contrast
objective, please see 2.2.4. You ought to mount it on the
turret.
4.1.2. Phase contrast slider (Figure 26)
oPhase centering adjustable slider
oThe light ring was centered beforehand, so it needn’t to
adjust in the use process. If the ring is not in the center, you
could adjust by the centering bolt.
oThe 10X/20X light ring ①is worked with the 10X, 20X
phase contrast objective, while the opening ②is used for
bright field.
4.1. Name of the components
4. PHASE CONTRAST VIEWING
Figure 25
Figure 26

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4.2.1. Installing the phase contrast slider (Figure 27)
1. Keep the slider ①face (the surface which had character)
up towards, then inserted it into the illumination system
from the right to the left like the figure showed.
2. Every light ring or opening has its own located position, so
you need to move them until you heard the “clicked” to
ensure the ring or the opening reach the center of the light
path.
3. When in the phase contrast observation, do keep the
aperture diaphragm adjustment lever ②on the position of
“O” (wide opening).
4.2.2. The centering ring (Figure 28 and 29)
Usually you needn’t the operation of centering. If
necessary, please accord to the following steps:
1. Place the specimen on the stage and focus it.
2. Take out the eyepiece, replace it with the CT (the centering
telescope), and inserted it into the viewing tube without
diopter adjustment.
3. Make sure the matched phase contrast objective and light
ring (in the phase contrast slider) have been in the center of
the light path.
4. Using the CT to look the light ring’s image ①and the
phase contrast ring’s image ②, if the light ring’s image is
not sharp, please shifting the CT’s ocular until you can see a
clear image of the light ring ②.
5. Adjusting the bolts of the two centering holes ③in the
phase contrast slider by the screwdriver ③until the light
ring center and the phase contrast center are coincided.
4.2. Installation and use
Figure 27
Figure 28
Figure 29

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6. The 10X and the 20X phase contrast objective use the same light ring on the phase contrast slider. So
you need to check the coincidence of the light ring center and the phase contrast center when changing
the objective. If having departure, you ought to center again.
 If the light ring is centering incorrectly, you will fail to obtain the best viewing effect of the
microscopy.
 After removing or replacing a thick specimen, the light ring and the phase contrast ring are likely
to deviate each other, which will result in a decline of the image contrast. So if happened, please
repeat the steps as above.
 If the container or the cover flip which used to place the specimen is not flat, it maybe need to
repeat the centering steps for obtaining a more contrast effect. Please center the light ring by the
phase contrast objective, according to the sequence of low to high magnification.

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5.1.1. Selecting the light path (Figure 30)
Just used in the trinocular observation
1. Pulling out the light path selector lever, until you heard the
“clicked”.
In the dark specimen observation, you can make the focus
by both eyes at first, then change the light path
5.1.2. Installing the video set (Figure 31)
1. Loosen the locking bolt ①on the trinocular viewing tube,
and take out the dust cap ②.
2. Remove the dust cover on the both ends of the video
accessories ③, and revolve the screw head end into the
CCD/CMOS port.
3. Install the accessories into the tri-through port, and screw
down the bolt ①.
5.1.3. Focus (Figure 31)
Doing a binocular observation at 20% brightness, look the image on
the video or the computer which connected with the microscope
video system when the image is sharp. If it is not in focus, please
turning the revolving video connected tube ④until the image is
sharp enough.
5.1. Microscope video
5. MICROSCOPE PHOTOGRAPHY AND VIDEO
Figure 30
Figure 31

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5.2.1. Selecting the light path
Just used in the trinocular observation
The operation diagram is shown in 5.1.1, and the details reference is
in 3.3.3.
5.2.2. Installing the photography set (Figure 32)
1. Loosen the locking bolt ①on the trinocular viewing tube,
and take out the dust cap ②.
2. Install the photography accessories ③into the tri-through
port, and screw down the locking bolts ①.
3. Inserted the camera gate which on the digital photography
connected head ④into the correspond position of the
camera set port, and screw it down clockwise.
4. Plug the digital photo connected head into the photo tube,
then screw down the locking bolts ①.
Before connecting the camera and adapter, remove the
camera lens firstly, then connect the lens port with the
adapter. Pay attention to the gate type, please.
To avoid the disturbing from the ocular in the observation,
please place the viewer finder on the two sides of the
microscope when installing the camera set.
The camera magnification = objective magnification ×
camera lens magnification
When shooting the micrograph, the lens close will bring an
impact in some camera. In order to weaken the impact, and
obtain a clear image, you could select a longer time of
exposure or decrease the brightness to have some
compensation.
This explanation is used for Nikon single-lens reflex digital
camera
5.2. Microscope photography
Figure 32

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5.2.3. Focus
Do the binocular observation at 20% brightness, and focus primary. When in microscope photography, do use
the camera viewfinder to focus the specimen. Please refer the user manual of the camera set to obtain the details.
5.2.4. Adjusting the color temperature
oWhen shooting the chromo photograph, please use the sunlight film.
1. Mount the LBD temperature changed color filter on to the color filter bracket.
2. Turn the brightness adjustment knob to the maximal position, so you obtain a sunlight illumination.