STEINDORFF NYMCS-702 User manual

STEINDORFF®
Inverted Fluorescence Microscope
NYMCS-702
Instruction Manual
This manual is written for Inverted Fluorescence Microscope NYMCS-702. For safety and for keeping the best
performance, making you familiar with the instrument entirely, it is strongly recommended that you read this
manual carefully before using the microscope.

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STEINDORFF®
User Notice ………………………………………………………………………………………………..
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1. Component Names …………………………………………………………………………………...
4
2. Installation ……………………………………………………………………………………………
5
2.1. Installing diagram ……………………………………………………………………………...
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2.2. Installment steps ………………………………………………………………………………..
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3. Adjustment and operation …………………………………………………………………………...
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3.1. Lamp adjustment for fluorescence observation ……………………………………………...
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3.2. Reflected observing illumination adjustment ………………………………………………...
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4. Microscope photography and video ………………………………………………………………...
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4.1. Microscope video ……………………………………………………………………………….
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4.2. Microscope photography ………………………………………………………………………
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5. Outfit ………………………………………………………………………………………………….
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5.1. Specification …………………………………………………………………………………….
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5.2. Objective Specifications ………………………………………………………………………..
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6. Troubleshooting ………………………………………………………………………………………
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CONTENTS

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Safety Note
1. The epi-fluorescent attachment is a precise instrument. Open the box carefully, and avoid dropping the
accessories to ground and causing damage to them.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and variations.
3. Make certain that the burner is installed correctly and all cords are connected firmly.
4. Do not open the lamp housing while it is turned on or for at least 10 minutes after it has been turned off.
Lamp housing parts are extremely hot and cause burns if touched.
5. Always be sure to ground (earth) the equipment.
6. Verify that the voltage and the frequency of the AC mains outlet match the setting of the voltage switch and
the frequency switch on the rear of the power supply unit.
7. Always use the power cord provided and make sure that the main switch is moved to “O” (OFF) before
connecting the power cord plug to the wall outlet.
8. To prevent any hazard, always turn the main switch on the power supply unit to “O” (OFF), unplug the
power cord plug from the mains outlet before replacing the burner or the fuse, and wait for at least 10
minutes before replacing the burner. (Be sure to use a GCQ-100 mercury burner.)
9. To prevent obstruction of the air flow, it is important to leave enough space around and above the lamp
housing.
Safety Symbol
Symbol
Meaning
The surface is very hot, not touch by your hands
Before using, please read the instruction carefully, improper operation will result in bodily injure or
instruction malfunction.
The main switch on
The main switch off
USER NOTICE

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Maintenance and Storage
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with
gauze slightly moistened with a mixture of ether (70%) and alcohol (30%).
Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be
sure to keep these chemicals away from open flames or potential sources of electrical sparks-for
example, electrical equipment that is being switched on or off. Also remember to always use these
chemicals only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the non-optical component of the equipment. To clean
these, use a lint-free, soft cloth lightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the power supply unit as malfunction or damage may occur.
4. In order not to impair the safety of the equipment, replace the burner when the counter of NFP-1 indicates
“100.00” hours. To prevent any hazard, always turn the main switch on the power supply unit to “O”
(OFF), unplug the power cord plug from the mains outlet, and wait for at least 10 minutes before replacing
the burner. High-pressure gas is sealed within the mercury burner. Thus, if it is continued to be used after
its service life expectancy, the glass tube may deform and may sometimes rupture.

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1. COMPONENT NAMES
Trinocular tube head
Phase contrast slider
Lamp
Eyepiece
Illumination bracket
Condenser
Protection barrier
Stage
Illuminator
Nosepiece
Stage inserted plate
Objectives
o4×
o10×
o20×
o40×
Body
Reflected fluorescence units
B excitation, G excitation
Fluorescence power supplier

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Lamp house
Condenser
illumination unit
Glass stage
Objectives
Power supply unit
Lever
Mechanical ruler
Body Stand
Protection barrier
Trinocular head
Eyepiece
Phase contrast slider
①
③
④
⑨
⑩
②
⑤
⑪
⑧
⑫
⑦
⑥
2.1. Installing diagram
The following figure shows the installation sequence of the components. The number in the figure shows the
installation steps.
Before installing, be sure every components is clean, do not score any parts or glass surface.
Keep well with the supplied hexagon wrench. When changing the components, you will need it again.
2. INSTALLATION

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2.2.1. Installing trinocular head (Figure 1)
Loosen the setscrew ②and insert the trinocular Viewing
Head ①into the body correctly, screw down with bolt ③.
2.2.2. Installing eyepiece (Figure 2)
Insert the eyepiece ④into tube until they are against.
2.2.3. Installing condenser set (Figure 3)
Install the condenser into the right direction (Figure 3).
2.2. Installing Steps
Figure 1
Figure 2
Figure 3

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2.2.4. Installing lamp house (Figure 4, Figure 5)
Insert the plug “A”into hole “A”of power cord, then insert
the plug B into the B hole of the condenser till there are
against. (Figure 6)
Replace lamp
1. Turn the switch to off position when using or need
replacement. Pull out the lamp house and then the
lamp after it is cool down completely.
2. Insert the new lamp softly to prevent damage.
3. Do not touch the lamp by hands to prevent
reducing lamp expectancy or explode. Clean the
fingerprint by wiping slightly moistened with ether.
2.2.5. Installing phase contrast plate (Figure 6, Figure 7)
1. Keep the slider ①face (the surface which had
character) up towards. Every light ring or opening
has its own located position, so you need to move
them until you heard the “clicked” to ensure the
ring or the opening reach the center of the light
path (as shown in Figure 7).
2. Turn the aperture diaphragm lever ①to adjust
aperture. Turn the diaphragm to a big aperture
when do phase contrast observation.
The light ring was centered beforehand, so it
needn’t to adjust in the use process. If the ring is
not in the center, you could adjust by the centering
bolt.
The 10X/20X light ring is worked with the 10X,
20X phase contrast objective, while the opening is
used for bright field.
Notices
Requirement：
1. Temperature: 0℃～40℃, max humidity: 85%.
2. High temperature and moisture will damage instrument and
affect performance.
3. Keep the instrument away from the dust environment, and
take the dust cover when no using.
4. Lay the instrument without vibration place.
Figure 4
Figure 5
Figure 6
Figure 7

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2.2.6. Installing the objective (Figure 8, Figure 9)
1. Turning the coarse fusing knob ①like the figure shows
till the nosepiece get to its lowest position.
For ensuring the safety of the instruction on
transportation, the nosepiece is located in the lowest
position and the tension adjustment collar ②is
adjusted in an appropriate tight tension while
leaving the factory.
2. Screw the lowest magnification objective on to the
turret from the nearside, then turn the turret clockwise,
mount other objectives according the magnification
sequence of low to high.
oMount objective like this way will make the change of
magnification to be very easy in using.
oIt also can install the objective through the stage
opening.
Clean the objective regularly, the objective used in the
inversed microscope is very sensitivity about dust.
Do cover all the unused holes with turret dust caps ③,
to prevent the dust and contamination entering inside.
When operating, use the low magnification objective
(4X or 10X) to search and focus the specimen at first,
then replace the higher magnifications if necessary.
When replace the objective, slowly turning the
nosepiece until you hear “clicked”, that means the
objective enter into the right position—center of the
light path.
2.2.7. Mounting the Mercury Burner (Figure 10, Figure 11)
1. Loosen the burner socket clamping screw ①, and
remove the burner socket. (Figure 1)
2. After removing the foam backstop ②, securely insert
the + pole (the wide head) of the specified mercury
burner ③to the lower terminal first and then the –pole
(the thin head) to the upper terminal, then tighten the
two socket clamping screws ④.
3. Close the burner socket with burner into the original
position and tighten the socket clamping screw ①.
Figure 8
Figure 9
Figure 10

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Be sure to use a GCQ-100 mercury burner.
Be sure to mount positive pole (the wide head) before
the other, or the damage to the burner may occur.
Never subject the burner to excessive force when
mounting the Mercury Burner.
Be careful and avoid leaving fingerprints or dirt on the
mercury burner. Attached stain may cause distortion in
glass which could result in a ruptured burner. If stained,
wipe it a way gently with clean gauze.
To prevent any hazard, always turn the main switch on
the power supply unit to “O” (OFF), unplug the power
cord plug from the mains outlet, and wait for at least 10
minutes before replacing the burner.
2.2.8. Assembly of the Fluorescent Attachment, Cable and
Cord Connections (Figure 12, Figure 13)
1. Mount the lamp housing into the other end of the
attachment and fix it with two screws ④.
2. Plug the connector ⑤from the burner socket securely
into the connector on the power supply unit and make
sure the cord is correctly connected. (Make sure that the
main switch ④of the power supply is set to “O” (OFF)
before connecting cables)
3. Connect the power cord connector ⑥into connector on
the power supply unit and make sure the cord is
correctly connected.
Verify that the voltage and the frequency of the AC
mains outlet match the setting of the voltage switch and
the frequency switch on the rear of the power supply
units and improper setting may degrade burner
performance, or in the worst case(although very rare ),
cause the burner to explode.
It is better to use the power cord provided by Labomed
and the same type power cord should be used if you lose
or damage the old one.
2.2.9. Fuse Replacement (Figure 12, Figure 13)
1. Set the main switch to “O” (OFF) and unplug the
power cord before replacing fuses.
2. Using a flat-blade screwdriver, remove each of the
fuses holder ⑦by tuning it counter- clockwise and
pulling out.
3. Replace both fuses with new ones.
Always use the designated fuses (8A). And make sure
the voltage of the fuse match the voltage of the AC
mains outlet.
Figure 11
Figure 12
Figure 13

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2.2.10. Installing the stage lengthen splint and the
mechanical ruler (Figure 14, 15)
oStage lengthen splint can be installed in either side of
the stage to enlarge the work surface. But you can’t
install the mechanical ruler together.
oGenerally, the mechanical ruler will be installed in the
right side for comfortable adjustment.
1. Installing the stage lengthen splint.
First, Screw the fixed bolt ①on to the splint, then
mount it on to the stage from right or left below,
screwing down it until it stay hard.
2. Installing the mechanical ruler.
Please install the ruler like the way of the stage splint.
2.2.11. Installing protection barrier, glass plate, lever
(Figure 16)
1. Install the protection barrier on the attachment by
tightening the screw ②.
2. Placing the glass plate to the right position.
3. Screw the lever to the inversed components under the
nosepiece.
Figure 14
Figure 15
Figure 16

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3.1.2. Function of button (Figure 1)
①Hour counter
②Ammeter。
③Excitation button
④Start reset button
⑤Voltage switch
3.1.3. Adjusting the diopter (Figure 2)
1. Look into the right ocular by your right eye, then
revolving the coarse focus knob to focus on the
specimen.
2. Then use your left eye to look into the left ocular. If the
image is not sharp, just use the diopter adjustment ring
①to adjust please.
There are ±5 diopter in the adjustment ring. The number
which the reticle on the eyepiece holder pointed is your
eye’s diopter graduation.
3.1. Lamp adjustment for fluorescence observation
3.1.1. Connecting power
Set the main switch of the power supply unit to “I” (ON). It will stabilize in 5 to 10 minutes after
ignition.
Some mercury burners may not ignite the first time the power is turned on due to variance in
production, and the safety mechanism in the starter in such a case. If this occurs, set the main switch
to “1” (ON), then press the starter reset switch on the front panel of the power supply and between 1
to 4 seconds are required for igniting the burner. Repeat as necessary.
To avoid shortening the burner life, do not turn the burner off within 15 minutes after ignition.
The burner cannot be re-ignited for about 10mimutes, that is, until the mercury vapor inside it has
cooled down and condenser to liquid.
Ensure that the hour counter is reset to “000.00” after replacement of the burner. And you can insert
a thin object such as a mechanical pencil tip into the reset hole on the front panel of the power
supply unit to press the internal switch
3. ADJUSTMENT AND OPERATION
Figure 1
Figure 2

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3.1.4. Adjusting the interpupillar distance (Figure 3, 4)
When observing with two eyes, hold on the left and right
prism holder, turn around the axis, adjusting the
interpupillar distance until the left and right fields of view
coincide completely.
The reticle on the interpupillar distance indicator ③,
pointed by the spot “.” ②on the eyepiece holder, shows
the scale of the interpupillar distance. (Figure 2)
The range of the interpupillar distance: 48～75mm.
3.1.5. Switching the light path (Figure 4)
oPulling out the light path selector lever ④by your thumb,
select the light path you needed.
oWhen in the binocular observation, pushing in the lever
until you heard a “clicked”. While in video or photography,
pulling out the lever until it reached the “clicked” position.
3.1.6. Mounting Auxiliary Stage (Figure 5)
1. When using mechanical ruler. Located the specimen by
moving the X,Y knob(120mmх78mm)
2. Use the standard specimen cover (1.2mm) for best
observation.
Carefully replacing objectives or else the objective will
touch the inserted glass plate when observing the
specimen by short-working distance objective.
Figure 3
Figure 4
Figure 5

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3.1.7. Centering the mercury burner (Figure 6-8)
oBefore proceeding to center the burner, wait for the arc
image to stabilize to protect against glare during arc
image centering, it should be viewed across the excitation
light protective shield.
1. Switch the light shutter ①to“●” position to shut off the
light path.
2. Revolve the filter block turret to engage the green or
blue excitation filter block into the light path. If U/V
excitation filter block used, be sure to use the protective
shield.
3. Revolve the nosepiece to engage 10× objective into the
light path. Place the centering plate on stage, through
transmission observation; adjust the stage until the cross
is in the center of the field of view.
4. Remove the objective from the revolving nosepiece
position and engage this position in the light path.
5. Pull out the field iris diaphragm lever ②to close the iris
diaphragm and push in the aperture iris diaphragm lever
③to open the iris diaphragm to the large limit.
6. Switch the light shutter ①to “O” position to open the
light path.
7. Turn the collector adjusting knob ④to project the arc
image on the centering plate and sharpen it. (A)
8. Revolve the burner adjusting knob ⑤to move the arc
image and the mirror reflected arc image in the
symmetrical position. (B)
9. Adjust the mirror focusing knob ⑥(Figure 6) to
sharpen the mirror reflected arc image. (C)
10. Turn the burner adjusting knob ⑤to overlap the arc
image with the mirror reflected arc image. (D)
oTurn the collector adjusting knob ④to make the field of
view as bright as regular as possible.
oMaintain this condition until the next time the burner is
replaced.
Figure 6
Figure 7
Figure 8

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Note:
When the hour counter indicates “100.0”, set the main switch to “o” (OFF) for safety, wait for more than
10 minutes, and then replace the lamp burner after making sure that the lamp housing has cooled down. A
mercury burner seals high-pressure gas inside. If the burner is used beyond its service life, stress may
accumulate inside the burner, and in the worst (but very rare) case, the burner could explode.
After replacing with a new burner, reset the hour counter, be sure to press the reset switch until “000.00” is
displayed.
Centering the mirror reflected image (Figure 6)
The mirror reflected image has been centered before leaving the factory. Do not adjust the knob ⑦please
if not necessary. Only when the burner has been centered precisely, can the knob ⑦be adjusted.
Note: once the knob is adjusted, the reflected mirror cannot be reconverted to the status when
leaving the factory.
Knob control: (Figure 6)
1. The middle knob ⑥is the mirror reflected image focusing knob which can sharpen the reflected image.
2. The knobs at both sides ⑦can adjust the up/down or left/right position of the mirror reflected image.

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3.1.8. Centering the Field Iris Diaphragm (Figure 9, 10)
1. Engage the 10×objective in the light path, and place the
specimen on the stage and bring into approximate focus.
2. Pull the field iris diaphragm lever ②out until the
diaphragm comes into the smallest state.
3. Use the hexangular wrench to adjust the two field iris
diaphragm centering screws alternately to move the
image of the diaphragm to the center. (Figure 2 show the
adjustment of diaphragm)
4. Push in the field diaphragm lever to open the diaphragm.
As this makes slight deviation noticeable, adjust the
centering precisely.
5. Enlarge the diaphragm until it just circumscribes the
field of view.
Adjusting the field iris diaphragm
The field diaphragm adjusts the diameter of the illuminating
beam to obtain good image contrast.
Keeping the field diaphragm stopped down to the smallest
required area for each observation makes it possible to prevent
color fading of areas outside the observation target region.
According to the objective in use, adjust the diaphragm image
using the field diaphragm lever so that the field of view is
circumscribed by the field diaphragm to exclude stray light.
Figure 9
Figure 10
a
b
c

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3.1.9. Using color filters (Figure 11)
oSelecting the appropriate color filters according your
need, it became more effective to observe or photography
the specimen. Especially, we suggest using the LBD
color filter, which can compensate more neutral colors.
oYou could pile up a group of color filters to the filter
holder, if you ensure they are level and the whole
thickness is less than 11mm.
Color Filter
Meaning
IF550
Single contrast color filter (green)
(used for the phase contrast microscopy)
LED
Color temperature transit color filter (blue)
(used for bright field observation and
microphotography)
3.1.10. Using the aperture diaphragm (Figure 12)
oWhen in the bright field observation, the aperture
diaphragm control the numerical aperture of the
illumination system. Only when the numerical aperture
of the objective and the illumination system being
matching, you can obtain the higher image resolution and
contrast, and the increased depth of field, too.
To recognize the aperture diaphragm, you could remove the eyepiece if necessary (You also could insert in
the center telescope), then looked into the viewing tube, you might see a field of view like the figure shown.
The proportion could be changed by dialing the aperture adjustment lever according your need. (①is the
image of the aperture diaphragm, ②is the edge of the objective).
Generally, when observing the chromatic specimen, you need to set the size of the condenser aperture
diaphragm at 70%～80% of the numerical aperture which marked in the objective, but if observing the
bacterium specimen which not colored, you could turn the aperture diaphragm lever at the direction of “ ”
(clockwise)
Figure 11
Figure 12

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3.2.1. Turn power, adjusting brightness (Figure 13)
Connect the power, turn on the main switch ①on the
bottom side of the base to “－”(on). Turning the
brightness adjustment knob clockwise ②, the voltage
raise, and the brightness strengthen; whereas turning at the
contra direction, the voltage decline, and the brightness
weaken.
oUsing the lamp in a low voltage condition, will prolong the
service life.
3.2.2. Adjusting the Tension Adjustment Collar (Figure14)
The tension of the coarse focusing knob ②has already
been adjusted properly before leaving factory.
oHow to adjust tension of the coarse focusing knob?
Turn the tension adjustment collar ①. While revolving at
the direction of the arrow in the figure, the tension of the
coarse focusing knob②is increasing, and if at the contra
direction, the tension will decline.
3.2.3. The centering ring (Figure 15, 16)
Usually you do not need the operation of centering. If
necessary, please accord to the following steps:
1. Place the specimen on the stage and focus it.
2. Take out the eyepiece, replace it with the CT (the centering
telescope), and inserted it into the viewing tube without
diopter adjustment.
3. Make sure the matched phase contrast objective and light
ring (in the phase contrast slider) have been in the center of
the light path.
4. Using the CT to look the light ring’s image ①and the
phase contrast ring’s image ②, if the light ring’s image is
not sharp, please shifting the CT’s ocular until you can see
a clear image of the light ring ②.
5. Adjusting the bolts of the two centering holes ③in the
phase contrast slider by the screwdriver ③until the light
ring center and the phase contrast center are coincided.
6. The 10X and the 20X phase contrast objective use the
same light ring on the phase contrast slider. So you need to
check the coincidence of the light ring center and the phase
contrast center when changing the objective. If having
departure, you ought to center again.
3.2. Reflected observing illumination adjustment
Figure 13
Figure 14
Figure 15
Figure 16
Knob

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If the light ring is centering incorrectly, you will fail to obtain the best viewing effect of the
microscopy.
After removing or replacing a thick specimen, the light ring and the phase contrast ring are likely to
deviate each other, which will result in a decline of the image contrast. So if happened, please repeat
the steps as above.
If the container or the cover flip which used to place the specimen is not flat, it maybe need to repeat
the centering steps for obtaining a more contrast effect. Please center the light ring by the phase
contrast objective, according to the sequence of low to high magnification.

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4.1.1. Selecting the light path (Figure 1)
Just used in the trinocular observation
Pulling out the light path selector lever, until you heard
the “clicked”.
In dark specimen observation, you can make the focus
by both eyes at first, then change the light path.
4.1.2. Installing the video set (Figure 2)
1. Loosen the locking bolt ①on the trinocular viewing
tube, and take out the dust cap ②.
2. Remove the dust cover on the both ends of the video
accessories ③, and revolve the screw head end into the
CCD/CMOS port.
3. Install the accessories into the tri-through port, and
screw down the bolt ①.
4.1.3. Focus (Figure 2)
Doing a binocular observation at 20% brightness, look the image
on the video or the computer which connected with the
microscope video system when the image is sharp. If it is not in
focus, please turning the revolving video connected tube ④until
the image is sharp enough.
4.1. Microscope video
4. MICROSCOPE PHOTOGRAPHY AND VIDEO
Figure 1
Figure 2