STEINDORFF CX50 Series User manual

INSTRUCTION MANUAL
360° Rotatable Head
Rubber Feet
Nosepiece
Achromatic color-coded
DIN Objectives
Double layer
Machanical Stage
Coaxial Coarse
and
Fine Focusing
Iris Diaphragm
LED Illuminator
Light Intensity Control
STEINDORFF®

CONTENTS
A.Illustration of Microscope…………………………….……..………COVER
B.Unpacking and Preparation for Use…..………….…...…….............
…… 1
C.Features / Denitions…………………………..……..…….............…….. 2
D.Operating Procedure…….…………………………….........................… 4
E.Maintenance and Care …….………………………………....…..…........ 6
F.Electrical Maintenance ………..………….……
...............….............. ..... 7
G.Troubleshooting….………
…….……………............. .................…...…...9
H.OptionalDigitalCameras…………………..……...........………...….…...10
- 1 -
B. UNPACKING & PREPARATION
Y our The microscope is supplied with an expanded two-part
Styrofoam/polystyrene case. This case should be used for
storage, transport, and shipping. Microscopes that are not
properly packed are usually damaged during transport and
shipping. KEEP STYROFOAM BOX FOR FUTURE USE
FOR SHIPPING AND/OR REPAIR.
Unpack the microscope and its parts carefully. Do not throw
away any boxes or packing materials until the contents of
the shipping container have been checked against the pack-
ing list of your order. In an area that is clean, dry and dust
free, place the microscope and parts on a sturdy desk or
table for initial use and/or assembly.
DO NOT TOUCH ANY LENS SURFACES WHILE HAN-
DLING THE MICROSCOPE; THIS MAY ADVERSLEY AF-
FECT IMAGE QUALITY.

C. FEATURES AND DEFINITIONS
EYEPEICE:
10X wide eld eyepiece with built in calibrated pointer for
measurement.
HEAD:
monocular head: 360° rotatable monocular head.
Binocular head: Dual view head allows 2 people to observe
the microscope simultaneously.
OBJECTIVES:
The lower functional component of the optical system. Avail-
able in 4X, 10X and 40XR, 100XR.
COAXIAL FOCUSING KNOBS:
The microscope is equipped with coaxial coarse (larger con-
trol) and ne focus (smaller control) knobs. Coaxial ne and
coarse adjustment enable you to perform two functions with-
out having to move your hands to a di󰀨erent position. The
reduction system allows for movement on a single ball bear-
ing dove-way for smooth and accurate focusing control. One
complete turn of the ne focus control is equal to a 1.8mm
vertical displacement of the stage.
CONDENSER:
The microscope is equipped with a 1.25 N.A. Abbe condenser
that has rack & pinion focusing, Iris Diaphragm, lter holder.
DIAPHRAGM:
The microscope is equipped with Iris Diaphragm. Its function
is to limit the amount of light transmitted to the objective for
preventing oversaturation, for which allows the amount of am-
bient light reaching the objective to be controlled to ensure the
high image e󰀨ect.
ILLUMINATOR:
LED illumination with intensity control, guarantees a clear and
- 2 -

crisp, cool-to-touch white light that lasts up to 60x longer than
traditional tungsten bulbs.
STAGE:
The stage of the microscope is equipped with built-in double
layer mechanical stage.
SAFETY RACK STOP:
Controls the maximum upward movement of the stage.
SPECIFICATIONS (With 10x Eyepiece)
Objective Total Magnication Field of View N.A.
4X 40X 4.5mm 0.10
10X 100X 1.8mm 0.25
40X 400X 0.45mm 0.65
100X Oil 1000X 0.18mm 1.25
D. OPERATING PROCEDURES
I. Microscope without an 1.25 Abbe-condenser
1. Select a position to work where little direct light falls on the
instrument. Avoiding placing the microscope near or facing
a large window as direct light may fall directly on the mi-
croscope and adversely a󰀨ect the contrast and resolution.
2. Plug it into any grounded receptacle.
3. Adjust the Iris diaphragm to control the amount of light
align with the in-stage condenser.
- 3 -

4. Place a low powered objective into position.
5. Place the micro-slide specimen to be observed under the
spring stage clips. If using a mechanical stage, pull back
the lever on the left side of the stage, insert the slide, then
bring the crescent shaped holder into contact with the slide.
Be certain that the cover slip of the slide is facing towards
the objective; otherwise you will not be able to focus your
specimen at high magnications.
6. Position the specimen so that it is centered over the in-
stage condenser.
7. Focus the objective on your specimen by turning the
LARGE COARSE ADJUSTMENT KNOBS until the image
of your specimen is bright and clear. Microscopists will al-
ways lower the objective to a point that they know is be-
neath the focal plane and focus upwards. Now you can
bring the specimen into sharp focus by turning the SMALL-
ER FINE FOCUS KNOBS.
8. With the specimen now in sharp focus, rotate the nose-
piece to the other objectives and focus using only the ne
focus knobs. Since the optics on the Microscopes are
Par-focal and Par-centered only a slight turn of the ne
focus knobs with be necessary.
NOTE: It is important to note that because of our built – in
stop the 4X and 10X objectives can never come into con-
tact with your microscope slides. The 40XR and 100XR may
occasionally touch the micro-slide but because these objec-
tives have retractable mounts your slide will not be damaged.
9. Adjust the Iris diaphragm until proper specimen contrast is
achieved.
10.You are now ready for microscope observation.
- 4 -

II. Microscope equipped with an 1.25 Abbe-condens-
er with or without the 100XR oil immersion objective.
1. Follow steps 1 through 6 and then:
2. FOCUSING THE CONDENSER. The sub-stage Abbe
condenser is mounted beneath the stage in a rack & pinion
focusing mount. Adjust the Abbe condenser by turning the
knob until the illumination of the eld is uniform.
3. Adjust the condenser iris diaphragm to match the N.A. of
the objective. This is done by rst closing the iris and open-
ing it slowly until the entire led is evenly and brightly lit
and in good focus. If the objective is changed to a higher
power, the iris must be adjusted to the new objective.
4. THE USE OF THE 100XR, OIL IMMERSION IS RE-
QUIRED FOR 100xR OBJECTIVES. Here is how to do it:
5. Lower the stage and spring out the objective to give your-
self room to work with. Place one or two drops of quality
immersion oil over the slide cover slip, which is needed
to gather enough light for viewing with 100xR. Spring the
100xR objective back in place again, and proceed by mov-
ing the stage up until the lens makes contact with the oil.
Continue (slowly) to focus down with coarse adjustment
until the color or a blurred outline of the specimen appears.
Now complete the focusing with the ne adjustment so that
your image details come into sharper focus.
6. When you are nished using the slides, ensure to clean
the oil o󰀨 of the slides and microscope lens by using lens
paper and cleaning solution.
7. In using the 100xR DIN objective, the most favourable res-
olution is obtained with the Abbe condenser nearly touch-
ing the slide specimen. Ideally, a drop of immersion oil is
placed between the condenser and the slide, as well as
between the slide and the 100xR DIN objective. Although
this practice is not always followed in routine study, it is the
only way to take full advantage of the inherent resolution
of the 1.25 NA Abbe condenser.
- 5 -

E. MAINTENANCE & CARE
Cleaning of the optical surfaces
Never take objectives or eyepieces apart. They should be
cleaned on the instrument since they are not easily removed.
To clean lens surfaces, rst remove dust using a soft brush
or blow o󰀨 with a small syringe. Use a cotton-tip applicator
and a small amount of xylene. Clean only the front lens ele-
ment of the objective and the top lens of the eyepiece. Wipe
again with a clean cotton-tip and, nally, blow or brush o󰀨 the
lens surfaces. The mirror or illuminator lens surfaces may
be cleaned in the same manner, but better results may be
obtained by wiping with a soft lint free cloth.
Cleaning and Lubricating of Mechanical parts
This type of maintenance should be done by an authorized
technician and will help insure many years of trouble free
use of your microscope.
Stage Elevation Caution - The stage is set at a standard
position for this particular optical system. Please exercise
caution if you need to elevate the stage. If the stage is raised
too high, the objective lens and the slide can easily be dam-
aged due to unintended contact, which could result in abra-
sions to each surface.
Empty Magnication
Keep in mind that there is a limit to meaningful magnication.
When you magnify an object beyond 1000x, it will continue
to get larger, but the resolution will stay the same. This will
actually make the image blurry and unclear, and it is called
empty magnication.
This is why it is not professionally recommended to change
the WF 10x eyepiece to a higher magnication eyepiece
such as the (WF 15x, 20x, or 25x), because 1000x magni-
cation is the maximum limit of clear magnication achieved
with a microscope.
- 6 -

F. ELECTRICAL MAINTANENCE
Charging/Re-charging NiMH rechargeable batteries.
(Rechargeable models only)
Your microscope is equipped with rechargeable Nickel Metal
Hydride (NiMH) batteries. Batteries should be charged prior
to rst use. Fully charge batteries, approximately 8 hours, by
plugging the supplied charger into a standard 110v outlet, and
attaching to the power inlet on the microscope.
You may also use the microscope while charging. Each com-
plete charge should give between 50-70 hours of use. Re-
chargeable batteries should give up to 500 recharge cycles,
depending on conditions. LED lamps will last a minimum of
10,000 hours under normal conditions.
If for any reason the light on your microscope does not appear
to be working, check the following:
1. Adjust the light intensity
switch to ensure that it is
not turned all the way down.
2. Check the Fuse – check to
ensure the fuse is not bro-
ken or burnt out.
- 7 -
WARNING
DISCONNECT POWER SUPPLY CORD FROM WALL
RECEPTACLE BEFORE REPLACING LAMP OR FUSE
!
Part No. LBB13

Rechargeable Battery Replacement
(Rechargeable models only)
1. Lay the microscope on its side on a at surface, and un-
screw the four rubber feet
2. Remove the base and lay on a at surface
3. Unscrew the two screws on both ends of the rectangular
plastic box
4. Remove the cover and replace the NiMH rechargeable
batteries
LED or Tungsten Halogen Lighting Replacement
(LED Models)
1. Lay the microscope on its side on a at surface
2. Ensure your microscope has been unplugged
3. Loosen the thumbscrew on the lamp housing cover and open
4. Remove the plastic pins on each side of the disc shaped
bulb with small pliers. Carefully remove the bulb by sliding
it out from under the metal tabs
CAUTION:
- Avoid contact with ngers as oils from skin can lead to break-
age or shorten the life of the lamp
- Do not look directly into the operating lamp for any period of
time; this may cause eye injury
- Halogen lamps operate at high temperatures that can cause
serious physical injuries
FUSE REPLACEMENT
The fuse holder is located on the back of the
microscope base. Unscrew the fuse holder
cap, remove fuse, and replace with a new
fuse rated 1.0 amp
- 8 -
Part No. FU102

G. TROUBLE SHOOTING
Objectives
If you are using any type of liquid on the slides and then view
through the microscope, make sure that you clean the ob-
jectives immediately after you are done. You can clean with
paper towel and xylene. If you do not clean after contact with
liquid the objective lens will become very dirty. This will greatly
a󰀨ect the viewing quality of the objective.
The eyepiece
If view is hindered, check for ngerprints or scratches on the
eyepiece. Do not scratch eyepiece with your ngernail, as
this will remove the optic coating. Only clean the outer lens
and do not attempt to take eyepiece apart. Clean by using a
can of compressed air meant for dusting, or breathe on lens
and wipe with lens paper.
Rack Stop Screw
This screw sets how far up the stage will move. Students
have a tendency to screw this all the way down. This limits the
upper movement of the stage, preventing objects from com-
ing into focus. Turn the screw back up. The more the screw
comes up the higher the stage is able to move upwards. Do
not turn the screw to far as the stage may hit the 40x and
100x objectives. When properly adjusted, the safety rack stop
prevents the objective lens from hitting the microscope which
can result in damage to the specimen slide.
Slide and Cover slip
If the slide does not come into focus check to see that the
slide is not upside down. Make sure the cover slide is facing
up. Also make sure the slide is not dirty; spots will show up if it
is. Also make sure that the cover slip over the specimen is not
too thick, use a 0.17mm thick cover slip over your specimen
- 9 -

H. OPTIONAL DIGITAL CAMERAS
Three digital cameras to choose from:
DG 0.35 / DG 1.3 / DG 3.0
The digital cameras are specically designed for use with any
standard biological or stereo microscope. It acts as a converter
by converting your analog microscope into a digital microscope
enabling you to capture, analyze or share the digital images. DG
Series digital cameras use the UVC design.
The 0.35, 1.3, or 3.0 mega pixel digital eyepiece cameras of-
fer USB 2.0 plug-and-play compatibility, and comes with a us-
er-friendly imaging software. Windows compatible
Our software is free and easy-to-use. Additional processing func-
tions provide strong support for your scientic research. Further-
more, this DG series digital camera allows 3rd party applications
to setup and control, giving you more options.
Includes:
•Digital Eyepiece Camera • USB 2.0 Cable (1.5m long)
•User Manual • CD with Software (ISCapture)
Camera Specications
DG 0.35 DG 1.3 DG 3.0
E󰀨ective pixels 0.35MP 1.3MP 3.0MP
Max. resolution
(H x V) 640 x 480 1280 x 1024 2048 x 1536
Image Sensor ¼ “ COMS 1/3” COMS ½” COMS
Pixel size 2.8 x 2.8 3.6 x 3.6 3.18 x 3.18
Frame Rate 40 fps
(640 x 480)
30 fps
(640 x 480)
30 fps
(640 x 480)
White Balance Manual/Auto
Sensitivity 1.0V/Lux-sec
Image Format JPG/BMP
Data Interface USB 2.0
Compatibility Win XP, Win Vista, Win 7, Win 8, Mac
- 10 -

100 Lauman Lane, Suite A, Hicksville, NY 11801
Tel: [phone] | Fax: [phone]
Email: [email protected]
www.microscopeinternational.com