Leica DMI6000 B User manual

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Leica DMI6000 B
Operating Manual

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Published October 2004 by:
Leica Microsystems Wetzlar GmbH
Ernst-Leitz-Straße
D-35578 Wetzlar (Germany)
Responsible for contents:
Bernard Kleine
(Marketing CM, Compound Microscopy, Product Manage-
ment)
Holger Grasse
(Safety Officer according to MPG §30) In case of questions, please contact the hotline:
Phone [phone]
Fax [phone]
E-mail: [email protected]

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Leica DMI6000 B
Operating Manual

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Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems Wetzlar GmbH. Reproduc-
tion of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (in-
cluding electronic systems) is not allowed with-
out express written permission from Leica Mi-
crosystems Wetzlar GmbH.
The term "Windows" may appear in the following
text without further identification. It is, however,
a registered trademark of Microsoft Corpora-
tion. The names of companies and products
used herein may be trademarks of their respec-
tive owners.
The instructions contained in the following doc-
umentation reflect state-of-the-art technology
and knowledge standards. We have compiled
the texts and illustrations as accurately as pos-
sible. Nevertheless, no liability of any kind may
be assumed for the accuracy of this manual’s
contents. Still, we are always grateful for com-
ments and suggestions regarding potential mis-
takes within this documentation.
The information in this manual is subject to modifi-
cation at any time and without notification.

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Contents
6.11 Installation of Lamp Housing Mount
and Mirror Housing ................................... 42
6.12 Installation and Replacement
of Incident Light Lamps ............................ 44
6.13 Equipping the Incident Light
Turret Disk ................................................... 48
6.14 Installation of the Polarizer and
Analyzer....................................................... 50
6.15 Optional Accessories ............................... 52
6.16 Connection to the
Electronics Box CTR6000 ......................... 53
6.17 Connection to the Computer ................... 54
6.18 Connection to the Power Supply ............ 54
7. Start-up........................................................ 55
7.1 Functional Principle .................................. 55
7.2 Switching on the Microscope................. 59
7.3 The LeicaScreen........................................ 60
7.4 The Function Buttons on the Stand ....... 61
7.5 The SmartMove Remote Control
Module......................................................... 64
7.6 Illumination ................................................. 65
7.6.1 Transmitted Light ............................ 65
7.6.2 Incident Light - Fluorescence ...... 68
7.7 Checking Phase Contrast Rings ............. 69
7.8 Setting the Motorized Polarizer............. 70
7.9 Adjusting the Light Sources .................... 71
Contents
1. Important Notes about this Manual ...... 7
2. Intended Purpose of the Microscope ... 8
3. Safety Notes ............................................... 9
3.1 General Safety Notes ............................... 9
3.2 Electrical Safety ........................................ 10
4. Overview of the Leica DMI
6000 B......... 12
4.1 Specifications ............................................ 12
4.2 Glossary....................................................... 16
5. Unpacking the Microscope .................... 22
6. Assembling the Microscope .................. 25
6.1 Assembly Tools .......................................... 25
6.2 Installation of the Transmitted Light
Illumination Carrier (DL) ........................... 26
6.3 Installation of the DIC Module
and DIC Objective Prisms ........................ 27
6.4 Installation of Specimen Stages............. 28
6.5 Installation of Condensers....................... 34
6.6 Installation of Eyepieces.......................... 39
6.7 Installation of Objectives ......................... 39
6.8 Installation of Filters
in the Illumination Arm ............................. 40
6.9 Installation of the
Transmitted Light Lamp Housing ............ 40
6.10 Installation and Replacement of the
Transmitted Light Lamps: .........................
Lamp Housing 107 or 107/2 ...................... 41

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Contents
8. Operation .................................................... 74
8.1 Switching On .............................................. 74
8.2. Contrast Methods...................................... 76
8.2.1 Brightfield (TL)................................. 76
8.2.2 Phase Contrast (TL) ........................ 77
8.2.3 Darkfield (TL) ................................... 77
8.2.4 Polarization (TL) .............................. 78
8.2.5 Differential
Interference Contrast (TL) ............ 79
8.3 Fluorescence.............................................. 80
8.4 Combination Methods .............................. 81
8.5 Focusing ...................................................... 82
8.6 Tubes............................................................ 84
8.7 Eyepieces .................................................... 85
8.8 Objectives ................................................... 85
8.9 Stages and Object Displacement ........... 88
8.10 Magnification Changer............................. 89
8.11 Light Sources ............................................. 89
8.12 Aperture and
Field Diaphragm ......................................... 90
9. Troubleshooting......................................... 91
10. Care of the Microscope ........................... 95
10.1 Dust Cover .................................................. 95
10.2 Cleaning....................................................... 95
10.3 Handling Acids and Bases ...................... 96
11. Major Consumable and Replacement
Parts ............................................................ 97
12. Dimensions................................................. 98
13. Abbreviations and Pictograms............... 99
14. Index ............................................................ 101
15. EU Declaration of Conformity ................. 104

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1. Important Notes about this Manual
(1.2)
→
p.20
!
*
Numbers in parentheses, such as "(1.2)", corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with pointer arrows (for example
→p.20), point to a certain page of this manual.
Caution!
Special safety instructions within this manu-
al are indicated with the triangle symbol
shown here, and have a gray background.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Explanatory note.
Item not contained in all configurations.
Text symbols, pictograms and their meanings:
Caution!
This operating manual is an essential com-
ponent of the microscope, and must be read
carefully before the microscope is assem-
bled, put into operation or used.
1. Important Notes about this Manual
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the microscope and
accessories. It must therefore be kept safely for
future reference.
A separate manual is available on CD-ROM cov-
ering the operation of the Leica Application
Suite (LAS).

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2. Intended Purpose of the Microscope
2. Intended Purpose of the Microscope
The Leica DMI 6000 B microscope described in
these instructions is intended for biological rou-
tine and research applications. This includes the
examination of samples taken from the human
body to provide information on physiological or
pathological states or congenital abnormalities,
or determing the safety and compatibility with po-
tential recipients, or monitoring therapeutic
measures.
The Leica DMI
6000 B is a further development
of Leica’s proven inverted research micro-
scopes. It is designed for cellular and tissue ex-
amination, micromanipulation and microinjec-
tion techniques, microdissection, and confocal
microscopy. The Leica DMI
6000 B is suitable for
universal deployment, all contrast methods such
as darkfield, brightfield, phase contrast, DIC, flu-
orescence, and modulation contrast are integral
to the microscope and can be adapted or
changed quickly and easily. Variable illumination
and imaging beam paths, as well as HCS optics,
modular accessories and a comprehensive
range of peripherals, complement the Leica DMI
6000 B inverted research stand.
The above-named microscope complies with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. It also conforms to the Council
Directives 73/23/EEC concerning electrical ap-
paratus and 89/336/EEC concerning electromag-
netic compatibility for use in an industrial envi-
ronment.
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscope for other purposes
than those for which it is intended or not us-
ing it within the specifications of Leica Mi-
crosystems Wetzlar GmbH.
In such cases the declaration of conformity
shall cease to be valid.
Caution!
This (IVD) device is not intended for use in
the patient environment defined by DIN VDE
0100-710. Neither is it intended for combin-
ing with medical instruments according to
EN 60601-1. If a microscope is electrically
connected to a medical instrument accord-
ing to EN 60601-1, the requirements de-
fined in EN 60601-1-1 shall apply.

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3. Safety Notes
3. Safety Notes
3.1 General Safety Notes
This safety class 1 device is constructed and
tested in accordance with
EN 61010-2-101:2002,
EN 61010-1:2001,
IEC 1010-1:2001,
Safety regulations for electrical measuring, con-
trol, and laboratory devices.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted whenev-
er the device is altered, modified, or used in
conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!

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3. Safety Notes
Caution!
Power plugs may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
Caution!
Peripheral devices with their own or sepa-
rate power supplies that are connected to
the microscope can have the same protec-
tive conductor potential by connecting them
to the ground screw on the back of the Leica
CTR6000 electronics box. For connections
without a ground connector, Leica Service
must be consulted.
Caution!
Never use any fuses as replacements other
than those of the types and the current rat-
ings listed here. Using patched fuses or
bridging the fuse holder is not permitted. The
use of incorrect fuses may result in a fire
hazard.
90-250V~
50-60 Hz
max. 290VA
T6.3 A
(IEC 60127-2/3)
15-35°C
max. 80% to 30°C
II
2
90-250V~
50-60 Hz
See CTR6000
See CTR6000
15-35°C
max. 80% to 30°C
II
2
90-250V~
50-60 Hz
max. 155VA
2xT2A (IEC 127)
10-36°C
max. 80% to 30°C
II
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3.2 Electrical Safety
General specifications
Leica CTR6000 Electronics Box
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
Microscope
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
ebq 100 Supply Unit*
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Overvoltage category:
Pollution degree:
(See enclosed manual)