Olympus CX43 User manual

INSTRUCTIONS
This instruction manual is for the Olympus biological microscope.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating this
microscope, and always keep this manual at hand when operating this product.
Retain this instruction manual in an easily accessible place near the work desk for future
reference.
Biological Microscope
CX43
A X 8878
Optical Microscope and Accessory

In accordance with European Directive on Waste Electrical and Electronic Equipment, this
symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in
your country.
NOTE: This product has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the product is operated in a commercial environment. This product
generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance
with the instruction manual, may cause harmful interference to radio communications.
Operation of this product in a residential area is likely to cause harmful interference in which case the
user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the product.
For Korea only
B급 기기 (가정용 방송통신기자재)
이 기기는 가정용(B급) 전자파적합기기로서 주로 가정에서 사용하는 것을
목적으로 하며, 모든 지역에서 사용할 수 있습니다.

Safety precautions .......................................................................................................................................1
1 Nomenclature of configuration units ......................................................................................5
2 Nomenclature of operating portions ......................................................................................6
3 Transmitted brightfield/darkfield observation methods ........................................7
4 Operating procedures of each portion ................................................................................9
4-1 Base portion .............................................................................................................................................................9
1 Turning ON the LED illumination ......................................................................................................................................9
2 Using the filter ..................................................................................................................................................................................9
4-2 Stage portion ........................................................................................................................................................ 10
1 Placing the specimen ............................................................................................................................................................10
2 Moving the specimen ............................................................................................................................................................. 11
4-3 Revolving nosepiece portion .................................................................................................................. 14
1 Selecting the objective .......................................................................................................................................................... 14
4-4 Focusing portion ................................................................................................................................................ 14
1 Focusing on the specimen ................................................................................................................................................ 14
2 Adjusting the tension of the coarse focusing knob ....................................................................................15
3 Using the pre-focusing lever ............................................................................................................................................15
4-5 Tube portion ........................................................................................................................................................... 16
1 Adjusting the interpupillary distance ........................................................................................................................16
2 Using the eye shades ............................................................................................................................................................16
3 Adjusting the diopter ............................................................................................................................................................... 17
4 Trinocular tube (U-CTR30-2) ............................................................................................................................................. 18
5 Tilting binocular tube (U-CTBI) ........................................................................................................................................18
6 Intermediate attachment .....................................................................................................................................................18
4-6 Condenser portion ........................................................................................................................................... 19
1 Selecting the observation method ............................................................................................................................. 19
2 Adjusting the aperture diaphragm ..............................................................................................................................20
4-7 Immersion objective ..................................................................................................................................... 21
1 Using the 100X oil immersion objective ................................................................................................................21
Contents

5 Various observation methods .....................................................................................................23
5-1 Transmitted phase contrast observation method .............................................................. 23
1 Centering the ring slit for phase contrast .............................................................................................................25
5-2 Transmitted simple polarization observation method .................................................... 26
1 Adjusting the crossed Nicol .............................................................................................................................................28
6 Image acquisition ..................................................................................................................................29
1 Camera adapter ..........................................................................................................................................................................29
7 Glossary of optical performance terminology .............................................................30
8 Troubleshooting ......................................................................................................................................32
9 Specifications ............................................................................................................................................34
10 List of optical performances .....................................................................................................35
11Assembly ....................................................................................................................................................37
11-1 Assembly diagram ....................................................................................................................................... 37
11-2 Assembly procedures ............................................................................................................................... 38
1 Attaching the intermediate attachment and the tube ...............................................................................38
2 Attaching the camera adapter ......................................................................................................................................38
3 Attaching the eyepieces (WHB10X, WHB10X-H).............................................................................................39
4 Attaching the eyepiece micrometer ..........................................................................................................................39
5 Attaching the objective .........................................................................................................................................................40
6 Attaching the specimen hold plate CX3-SHP or the specimen holder CX3-HLDT .........41
7 Attaching the polarizer (U-POT) .....................................................................................................................................41
8 Connecting the AC adapter and power cord ...................................................................................................42
9 Attaching the allen wrench holder .............................................................................................................................43

1
CX43
If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
product may also be damaged. Always use the product according to this instruction manual.
The following symbols are used in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury.
NOTE
: Indicates a potentially hazardous situation which, if not avoided, may result in
damage to the product or other property.
TIP
: Indicates the useful knowledge or information for use.
Safety precautions
Wear protective equipment such as gloves, etc.
When you observe the specimens which have potentiality of infection, wear protective equipment such as gloves, etc.
to prevent the specimens from touching your skin directly.
When you maintain the product which may have contacted the specimens which have potentiality of infection, wear
protective equipment such as gloves, etc., or clean the product before operation.
After observation, clean the portions where specimens contacted directly.
Remove the specimen when moving the product.
When moving this product, be sure to remove the specimen first, since it is in danger of dropping and spattering.
In case the specimen is damaged, promptly take the infection prevention measures.
When disposing of the product, follow the regulations and rules of your local government.
When disposing of the product which contacted the specimens which have potentiality of infection, follow the
regulations and rules of your local government.
Install the microscope on a sturdy, level table or bench.
For safety in particular, do not place a mat, etc. under the product.
CAUTION - Installation of the product -
CAUTION - Prevention of infection -

2
CAUTION - Electric safety -
CAUTION - LED (light emitting diode) -
Do not look directly at the light from LED for a long time.
If you feel that the light from LED is too bright during observation, adjust the light intensity using the brightness adjustment
knob before continuing the observation. The LED built in this product is basically eye-safe. However, do not look directly
at the light from LED for a long time while feeling too bright, since it may cause damage to your eyes.
CAUTION - Safety symbols -
Always use the AC adapter and power cord provided by Olympus.
If the proper AC adapter and the power cord are not used, the electric safety and the EMC (Electro-Magnetic
Compatibility) performance of the product cannot be assured. If no power cord is provided, please select the proper
power cord by referring to the section “Proper selection of the power cord” at the end of this instruction manual.
Always connect the ground terminal.
Check that the ground terminal of the power cord and that of the power outlet are connected properly. If the product is
not grounded, our intended electric safety and EMC performance of the product cannot be assured.
Do not use this product near the sources of strong electromagnetic radiation.
Proper operation may be interfered. Be sure to evaluate the electromagnetic environment before operating the product.
Disconnect the power cord in case of emergency.
In case of emergency, disconnect the power cord from the power cord connector of the product or from the power
outlet.
Install the product at the location where you can reach the power cord connector or the power outlet with your hand to
disconnect the power cord immediately.
This product complies with the emission and immunity requirements described in IEC61326 series.
The following symbols are attached to the product.
Study the meaning of the symbols and always use the product in the safest possible manner.
Symbol Meaning
Indicates a non-specific general hazard. Follow the description given after this symbol
or in the instruction manual.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
CAUTION - Light from the objective -
Do not look directly at the light coming out from the objective or the light reflected from the specimen.
Be careful about the light coming out from the objective, since not only visible light but also light of invisible wavelengths
(such as ultraviolet and infrared) may be emitted depending on the illumination methods.

3
CX43
Handling precautions
·This product is a precision instrument. Handle it with care and avoid subjecting it to a sudden or
severe impact.
·Never disassemble any part of the product. Otherwise, failure could be caused.
1. Be careful not to apply the impact or let the dirt/dust attached to the microscope when carrying it.
2. Do not use the product in areas where it may be subjected to direct sunlight, high temperature and/or humidity, dust
or vibrations. (For conditions of operating environments, see "9 Specifications" on page 34.)
3. When installing the microscope, assure sufficient space around the microscope.
4. When carrying this microscope, disconnect the AC adapter from the microscope, and store the AC adapter and the
power cord in the microscope frame. Then, hold both sides of the arm as shown in the lower left picture, and carry it
carefully. Then, hold both sides of the arm a as shown in the picture on the left, and carry it carefully. (For procedures
to store the AC adapter and the power cord, see "Storing the AC adapter and power cord" on page 42.)
NOTE
·Do not hold the stage b or the tube c, since they may
be damaged.
·When carrying the microscope, be sure to remove the
specimen and filters in advance, since they are in a risk of
falling. Also, be careful not to touch the objective.
·Do not carry the microscope keeping the AC adapter
connected to the microscope. The AC adapter or the
microscope may be damaged by hitting the output
connector of the AC adapter.
NOTE
c
b
a

4
Maintenance and storage
1. Do not leave stains or fingerprints on the lenses and filters. If they get dirty, blow away dust with a commercially
available blower and gently wipe the lens or filter with a piece of cleaning paper (or clean gauze). Only when cleaning
fingerprints and oil stains, slightly moisten a piece of cleaning paper with commercially available absolute alcohol
and wipe them off with it.
Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away
from open flames or potential sources of electrical sparks. For example, the electrical equipment that
is switched ON and OFF may cause the ignition of a fire. Also, always use absolute alcohol only in a
well-ventilated room.
2. Wipe the portions other than the lens with a dry soft cloth. If the dirt cannot be removed by dry-wiping, moisten a soft
cloth with diluted neutral detergent and wipe the dirty surface with it.
Do not use organic solvents because they may deteriorate the coated surface or plastic parts.
3. After using this product, store it in a dry place or cover it with a dust cover. If you need the dedicated dust over (made
by Olympus), contact Olympus.
Do not use a highly sealable cover, such as a plastic bag, etc. as a dust cover. The humidity in the
microscope may increase to damage the product.
4. When disposing of this product, be sure to follow the regulations and rules of your local government. Contact Olympus
for any questions.
CAUTION
NOTE
NOTE
Intended use
This product has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
This includes the observation of living cells or of specimen taken from tissues to gain physiological or morphological
information at hospitals or laboratories. Typical field of applications are genetics, human blood and tissue examination,
neurology, pharmacology and cellular biology.
Do not use this instrument for any purpose other than its intended use.
This product complies with the requirements of directive 98/79/EC concerning in vitro
diagnostic medical devices. CE marking means the conformity to the directive.
This product is applied with the requirements of EMC standard IEC/EN61326-2-6 and
IEC/EN61326-1 concerning electromagnetic compatibility.
This product complies with the emission and immunity requirements described in IEC61326
series. The electromagnetic environment should be evaluated prior to operation of this product.

5
CX43
1 Nomenclature of configuration units
· The units described on this page are configuration units of the CX43 system including options.
· For other combinable units, refer to the latest catalogs or contact Olympus.
TIP
Tube
Eyepieces
Revolving
nosepiece
Stage
Specimen holder
Power cord
AC adapter
Objective
Microscope frame
Intermediate attachment
Condenser

6
2 Nomenclature of operating portions
Y-axis knob
Revolving nosepiece
X-axis knob
Main switch
: Power is ON.
: Power is OFF.
Fine focusing knob
Coarse focusing knob
Aperture diaphragm
lever
Specimen holder
Condenser turret
Brightness adjustment knob
Diopter
adjustment ring
Binocular portion
Main switch
X-axis knob
Fine focusing knob
Y-axis knob
Y-axis knob
Fine focusing knob
Brightness adjustment knob
Various filters
Pre-focusing lever
Condenser top lens
Ring slit centering knob
Coarse focusing knob
Ring slit centering knob
Ring slit centering knob
Aperture diaphragm
Aperture diaphragm
lever
Tension adjustment ring
Tension adjustment ring
Tension adjustment ring
Aperture diaphragm
fixing knob
Specimen holder
fixing knobs

7
CX43
3 Transmitted brightfield/darkfield observation methods
  (Operation portion)  (Page)
2 Condenser turret 19
5 Revolving nosepiece 14
Observation
b Various filters 9
1 Main switch 9
3 Specimen holder 10
4 X-axis/Y-axis knobs 11
6 Coarse/fine focusing knobs 14
7 Brightness adjustment knob 9
8 Binocular portion 16
5 Revolving nosepiece 14
6 Coarse/fine focusing knobs 14
a Aperture diaphragm lever 20
9 Diopter adjustment ring 17
7 Brightness adjustment knob 9
Place the specimen on the stage.
Engage the 10X objective in the light path.
Engage the objective you use in the light path and bring
the specimen into focus.
Bring the specimen into focus and adjust the brightness.
Set the main switch to (ON).
Place the necessary filter.
Adjust the brightness.
Select BF (brightfield observation).
This section describes the procedures of the transmitted brightfield/darkfield observation, which is the basic of all observations.
The transmitted phase contrast observation and transmitted simple polarization observation are explained in "5 Various observation
methods" on page 23.
If the analyzer for simple polarization and the polarizer are engaged in the light path, remove them from the light path.
2 Condenser turret 19
For darkfield observation, select DF (darkfield observation).
For brightfield observation, adjust the aperture diaphragm.
Adjust the interpupillary
distance.
Adjust the diopter.

8
Make a copy of this observation procedure guide and put it near the microscope to use for observation.
TIP
1
2
3
4
5
6
9
7
b
a
8

9
CX43
1 Turning ON the LED illumination
Set the main switch a to (ON).
Rotating the brightness adjustment knob b in the arrow direction
increases the brightness and rotating it in the opposite direction
decreases the brightness.
1
2
4-1 Base portion
4 Operating procedures of each portion
2 Using the filter
Engage the filter in the light path if necessary
Engage the filter in the filter holder (window lens a) of the base portion
of the microscope frame.
One to several filters of 45mm in diameter can be inserted in
stack.
(Insertable thickness: up to 1.6mm)
If it is difficult to attach the filter due to the narrow space,
rotate the coarse focusing knob to raise the stage.
1
TIP
NOTE
a
b
a

10
1 Placing the specimen
4-2 Stage portion
When using the specimen holder for observing one slide glass
Rotate the coarse focusing knob a in the arrow direction to fully
lower the stage.
Press the specimen holding lever knob b backward (arrow direction)
to open the lever c, and slide the specimen from front to back on
the stage to place it.
After placing the specimen, return the specimen holding lever c
gently.
Place the specimen carefully. If you return the specimen
holding lever c with excess force or release your hand
from the specimen holding lever knob b in the middle,
the lever may collide with the specimen to damage it.
Placing the specimen with one hand
Rotate the coarse focusing knob a in the arrow direction to fully
lower the stage.
Place the specimen on the front side of the stage.
Spread the specimen holding lever c at the edge of the specimen
as shown in the picture on the left, and slide the specimen from front
to back on the stage to place it.
1
2
3
NOTE
1
2
3
b
For procedures to attach the specimen holder (option), see
page 41.
When using the specimen hold plate CX3-SHP (Option)
Rotate the coarse focusing knob a in the arrow direction to fully
lower the stage.
Place the specimen on the resin sheet b.
·The outside of the aperture c of the resin sheet is not
illuminated by the light from the condenser, the specimen
cannot be observed.
When operating the X-axis knob, be careful not to cover
the aperture of the stage with the resin sheet.
·The specimen hold plate cannot be used in combination
with the 100X oil immersion objective because the optical
performances cannot be delivered sufficiently during
observation using the 100X oil immersion objective.
·The specimen hold plate cannot be used for the darkfield
observation because the shadow appears in the center of
the field of view during darkfield observation.
For fixing the stage, see page 12.
TIP
1
2
NOTE
TIP
b
c
b
a
a
a
c
c

11
CX43
Rotating the lower X-axis knob a moves the specimen in the X-axis
direction (right and left).
Rotating the upper Y-axis knob b moves the specimen in the Y-axis
direction (front and back).
Stage movable range: Depth 52 mm x Width 76 mm
·Do not move the specimen by touching the specimen
holder directly by hand, since it will damage the rotary
mechanism of the knobs.
·The rotation torque of X-axis and Y-axis knobs become
heavier near the end of movable range of X-axis and
Y-axis. In this case, stop rotating the knob.
· If the stage knob rubber c is attached and it is difficult to
operate the stage, remove the stage knob rubber. If you want
to change the height of the stage knob rubber, move the stage
knob rubber up and down to adjust it.
· When attaching the stage knob rubber, insert the larger one to
the Y-axis knob (upper side) and the smaller one to the X-axis
knob (lower side) from below.
1
2
NOTE
TIP
a
c
b
2 Moving the specimen
When using thethe specimen holder CX3-HLDT (Option)
Rotate the coarse focusing knob a in the arrow direction to fully
lower the stage.
Press the specimen holding lever knob b backward (arrow direction)
to open the lever c, and slide the specimen from front to back on
the stage to place it.
After placing the first specimen until it touches the end, place the
second specimen so that it touches the first specimen.
After placing the specimens, return the specimen holding lever c
gently.
Place the specimen carefully. If you return the specimen
holding lever c with excess force or release your hand
from the specimen holding lever knob b in the middle,
the lever may collide with the slide glass to damage it.
1
2
3
4
NOTE
b
c
a

12
Scale of specimen position
By reading and taking a note of X-axis and Y-axis scales of the observation
position on the specimen, you can return to the original observation position
easily even if the specimen is moved.
< Reading the scale >
Read the "0" position of the outside scale (vernier scale) by the inside
scale (main scale).[a]
The scale indicates the unit of 1 mm.
Read the position where the outside scale line coincides with the
inside scale line by the outside scale (vernier scale).[b]
The scale indicates the unit of 0.1 mm.
The position shown in the left picture can be read as
137.8mm.
1
2
TIP
Fixing the stage
If you want to move the observation position by moving the specimen with
your finger without using the specimen holder, the stage can be fixed so
that it does not move unexpectedly.
Move the X-axis/Y-axis knobs to match the hole a at the back right
of the stage with the screw hole b.
Put the stage fixing knob c through the hole a, and screw it in the
screw hole b to fix the stage.
The stage fixing knob is provided with the microscope.
Be sure to store the stage fixing knob in the safe place.
1
2
NOTE
b
a
c
ab

13
CX43
b
a
How to track the
image that moves
to the left (how to
move the image to
the right)?
Move the
specimen to the
front.
How to move
the image in the
lower area to the
upper area?
Move the
specimen to the
left.
Direction of moving the image
How to track an observation image
The up-down and left-right movements of the image
observed through eyepieces are opposite to the
movements of the specimen.
NOTE
Slide glass
It is recommended to use the following slide glassesa.
Length: 76 mm, Width: 26 mm, Thickness: 0.9 to 1.4 mm
(Thickness: 0.9 to 1.2 mm for darkfield observation)
Use the cover glass b with the thickness of 0.17mm to
fully deliver the performance of objective.
NOTE

14
1 Focusing on the specimen
Rotate the coarse focusing knob a in clockwise direction (arrow
direction) facing the microscope from right side to move the objective
c to the specimen as close as possible.
While observing the specimen through the eyepieces, slowly rotate
the coarse focusing knob a in the direction opposite to the arrow
direction to lower the stage.
When the specimen comes into view, rotate the fine focusing knob
b to bring the specimen into focus precisely.
1
2
3
4-4 Focusing portion
4-3 Revolving nosepiece portion
1 Selecting the objective
Hold the revolving nosepiece a and rotate it so that the intended
objective comes exactly above the specimen.
·Do not rotate the revolving nosepiece by holding the
objective.
·Be careful if you rotate the revolving nosepiece while
observing the edge of the slide glass with the high
magnification objective (40X, etc.), the objective may
interfere with the specimen holder.
For attaching and removing the objectives, see page 40.
1
NOTE
TIP
c
a
b
a

15
CX43
3 Using the pre-focusing lever
The pre-focusing lever prevents the specimen from being damaged by
collision between the specimen and objective.
After bringing the specimen into focus with the high magnification
objective, rotate the pre-focusing lever a in the arrow direction until
it stops.
To provide a certain margin for focusing range, rotate the pre-focusing
lever a by about half turn in the direction opposite to the arrow
direction from the stopped position.
1
2
If the pre-focusing lever is not positioned appropriately,
the stage may not rise and the specimen may not be
focused. In this case, adjust the pre-focusing lever
position again.
It is recommended to use the pre-focusing lever to prevent the
specimen from damage, but if you do not use the pre-focusing
lever, rotate the pre-focusing lever in the direction opposite to
the arrow direction until it stops.
NOTE
TIP
The tension of the coarse focusing knob can be adjusted.
Insert a commercially-available flathead screwdriver into the groove c of
the tension adjustment ring. Rotating it clockwise (arrow direction) increases
the tension and counterclockwise decreases the tension of the coarse
focusing knob a.
If the stage descends by its own weight or the focus obtained
with the fine focusing knob b is lost soon, the tension is set
too loose. In this case, rotate the tension adjustment ring in the
arrow direction to increase the tension.
TIP
2 Adjusting the tension of the coarse focusing knob
c
b
a
a

16
4-5 Tube portion
1 Adjusting the interpupillary distance
The interpupillary distance adjustment is to adjust the distance between
two eyepieces according to the distance between your eyes. This makes
it possible to observe a single image and to reduce eye strain during
observation.
Keep the right and left eyepieces horizontally. While looking through
the eyepieces, move the binocular portion either in a or b direction
until the right and left fields of view coincide completely. The value
shown by the indicator (·) c on the eyepiece sleeve represents your
interpupillary distance.
Be sure to make the interpupillary distance same between
right and left.
Note your interpupillary distance so that it can be easily adjusted
next time.
1
NOTE
TIP
When wearing eyeglasses
Use the eye shades in the normal, folded-down position.
When not wearing eyeglasses
Extend the folded eye shades in the arrow direction. Since the eye shades
prevent the unnecessary light from entering between eyepieces and eyes,
you can observe the specimen comfortably.
2 Using the eye shades
a
b
c