D. Bi-Annual Preventative Maintenance.......................................................................................................................33
X. Troubleshooting............................................................................................................................................................34
A. Technical Support.....................................................................................................................................................35
Table of Figures
Figure 1. The ICELL8 cx instrument....................................................................................................................................10
Figure 2. Water Bottle seated on the Electronic Scale..........................................................................................................11
Figure 3. Humidifier unit......................................................................................................................................................11
Figure 4. Syringe Pumps Unit...............................................................................................................................................12
Figure 5. ICELL8 cx Stage Module......................................................................................................................................12
Figure 6. ICELL8 cx workflow.............................................................................................................................................15
Figure 7. Example of DewPoint parameter display. .............................................................................................................17
Figure 8. Wash Container. ....................................................................................................................................................18
Figure 9. Startup tab..............................................................................................................................................................18
Figure 10. Daily checklist.....................................................................................................................................................18
Figure 11. The [Tip Clean] button in the Manual Control section........................................................................................19
Figure 12. Source plate map. ................................................................................................................................................20
Figure 13. Single Cell / TCR tab...........................................................................................................................................20
Figure 14. Placing the chip on the Dispensing Platform.......................................................................................................21
Figure 15. Empty plate nest for the sample source plate. .....................................................................................................21
Figure 16. View of the ICELL8 cx with the front door closed and open..............................................................................22
Figure 17. Select the 3’DE / TCR tab. ..................................................................................................................................22
Figure 18. Vacuum status confirmation................................................................................................................................23
Figure 19. Confirm plate and chip loading. ..........................................................................................................................23
Figure 20. Confirm seal removal. .........................................................................................................................................23
Figure 21. Live image of chip scanning................................................................................................................................24
Figure 22. Input chip ID........................................................................................................................................................24
Figure 23. Vacuum status after dispense. .............................................................................................................................24
Figure 24. Initiate chip scanning...........................................................................................................................................25
Figure 25. Remove the chip seal...........................................................................................................................................25
Figure 26. Enter the chip ID after autofocus and barcode scanning steps. ...........................................................................26
Figure 27. Select analysis setting and barcode gal files........................................................................................................26
Figure 28. Select path and file name for saving data............................................................................................................27
Figure 29. Naming and saving results file. ...........................................................................................................................27
Figure 30. Cell / RT dispense and scanning..........................................................................................................................29
Figure 31. RT-PCR mix Source Plate map...........................................................................................................................29
Figure 32. Initiate RT dispense.............................................................................................................................................29
Figure 33. Loading filter files...............................................................................................................................................30
Figure 34. Confirm removal of sealing film. ........................................................................................................................31
Figure 35. Live image of chip scanning................................................................................................................................31
Figure 36. Enter chip ID. ......................................................................................................................................................32
Figure 37. Confirm that chip ID matches filter file name.....................................................................................................32
