Takara Bio Thermal Cycler Dice Touch User manual

TAKARA BIO INC
.
TaKaRa PCR Thermal Cycler DiceTM Touch
Ver.2.0.1
PCR Program Editor:
Configure the PCR Program
PCR File Manager:
For managing the PCR run files on the device
Manual Incubation:
Use for incubator
Configuration:
Optional settings of the unit
The Power Switch locates on the rear of the unit to put on or shut off the Power supply.
※Avoid to touch the screen and shut off the power supply until the screen appear the 'Home' screen. That is the cause of failure the
unit and wrong movement.
···
·Instructions and installations about TaKaRa Thermal Cycler DiceTM Touch is confirmed the content of User’s Manual .
·
The stylus[touch pen] (that is optional of the unit) is used to operate the program on the Touch Panel.
【Caution】·The beginning to use TaKaRa Thermal Cycler DiceTM Touch，you may read this Quick Guide and User’s
Home Scree
１．Prepare new user acountment and folder ···２
２．Create new PCR program ···
３．Edit the PCR program ···
４．Load the configured PCR program ···
５．Setting of specific measurement ···
６．Manual Incubation ···
．Optional setting ···

Dice Touch Quick Guide -2
TAKARA BIO INC.
１．Prepare new user acountment and folder
Prepare new user accountment
Touch a 'Configuration' from the Home screen.
Touch an 'User Accounts' icon from
Configuration.
Touch an 'Add User' on the operating icons
under the user accounts screen.
Enter an user name.
If you wish to set the password, you will check
the checkbox for 'Password Protect User' and
type your password of a four-degit integer
Choose an user profile image.
Identify to create a new user account.
Click to utilize the user icon in user accounts.
Check to note the log in user account name
on the 'Currently logged in :'.

Dice Touch Quick Guide -3
TAKARA BIO INC.
２．Create new PCR Program
Touch the 'PCR Program Editor' from the Home screen.
Show a standard program(named Program1)
on the screen.
Touch the 'Wizard' icon of PCR Program Editor.
Edit the 'PCR Experiment Wizard' screen.
Create new program that note the temperature,
the reaction time and number of cycles etc.
※If you keep on cool the reacted solution after PCR
reaction, you would check 'Hold on completion' .
Regulate the content
of Wizard and touch
the '✔' button.
When you select the 'Save ' icon, you are
assigned the User Accounts that save the
program method and enter the program
name .
Note the User Accounts and saved program
name at the upper left of PCR program.
Touch the ’Run File' icon of PCR Program Editor.
Confirm the content of program method on
the 'Confirm Run' screen and select the
'✔Start Run' button.

Dice Touch Quick Guide -4
TAKARA BIO INC.
３．Edit the PCR program
Touch a 'PCR Program Editor' from Home screen.
Change the User Accounts to click this part.
Show a standard program on the screen.
Touch an 'Load File' icon on the bottom shelf.
Select a objective program file to load and
click' ✔'button.
Touch a column of temperature, reaction time
and number of cycles. Appear the numeric
keypad screen to enter numeral of each column.
Touch an 'Add Step' icon, Add new event
after positive blue column or the end of
profile or start of profile that you select.
Check 'Insert after xx Within Cycling',
Insert to select a 'Hold' or 'Pause' step after
positive blue column within cycling
Pause event hold the temperature at the last
set point and display time is 'Infinite'.
Hold event is a simple hold at given
temperature for a set time.
Regulate the time rates reaching the set temperature
to check 'Use Custom Setting' on 'Limit the Ramp Rate
to the Temperature'.

Dice Touch Quick Guide -5
TAKARA BIO INC.
Check 'Add to end of Profile' or 'Insert at start
of Profile' or Insert after xx without cycling.
The first screen of the Add Event Wizard:
● Cycling (2～10 Step)
● Hold
● Ramp (≦2.5℃/sec)
● Pause
Cycling x Step will proceed to generate a multi-step cycling event.
Change the number of steps for the cycling event.
Input the number of steps between Within the cycling configuration screen there are
2 and 10. numeraous options that input temperature, hold
time and so on.
Ramp event allows the block temperature to be ramped either up or down between
two set temperatures over a preset length of time at temperature change less than
or equal to 2.5℃/sec.
If the Ramp rate is more than 2.5℃/sec,
the system message that refer to the picture on the right is appeared.
Step Options is supported for specific measurement (p.9) and
moves the positive blue step for left or right place.

Dice Touch Quick Guide -6
TAKARA BIO INC.
Touch a button of 'Preheat' to preheat top heater.
Press OK✔to turn top heater ON, CANCEL×to turn top heater OFF on System Message.
Change the 'Preheat' to red button
of 'Heating' with top heater heating.
Change temperature of top heater
to touch a button of [105].
Set top heater temperature from
60° to 115°.
Touch a icon of 'Run File' Confirm the content of running program
to run the setting program.
and touch the 'Start Run' ✔button then
start it.
※If you want to turn the temperature off
you have to touch a Configuration on
top heater on running program, the Menu and select a machine settings.
Uncheck the 'Use Top Heater' check box and Done.
Appear the system message to select CANCEL×
that turn top heater OFF running program.

Dice Touch Quick Guide -7
TAKARA BIO INC.
４．Load the configured PCR program
Touch a 'PCR File Manager' from Home screen.
Select an User Account for use or an
USB drive from Accounts on the PCR
File Manager.
Appear some program files(.t3p) at the
User Account for use.
※This software is available for .s3p filename
extension until version 1.1.2.
But new .t3p extension is not available for old
software.
Touch a file name in some program files,
appear the specification of this file.
Edit File： Edit the contents of the program file.
Rename：Change the name of a program file.
Put a cursor position on the rename program file, click 'Rename' icon. Input new file name using keyboard.
Caution: Disappear the original file name.

Dice Touch Quick Guide -8
TAKARA BIO INC.
Copy： Copy the program files.
Copy plural program files surrounding
them with a drag.
Paste： Paste the copied program files.
Select an account that want to make
copied files.
Delete：Delete the program files.
Confirm plural delete files and click OK.
History：Note the Name, Date run and Result of recently run program
on an User account.
Click the program name and select
Run program Again' then restart a
chosen program.
Remain 10 post records on a PostRunReport
folder.
Click a PostRunReport file, Appear the content
of post record. ⇒ Enable move for USB drive.
※Not available for rename of PostRunReport file.
The PostRunReport file change the text file to .t3p file.
This file is not able to open and show the error message.
Run Files: Run the program file.

Dice Touch Quick Guide -9
TAKARA BIO INC.
５．Setting of specific measurement
Touch a Step Options/Advanced Edit.
１．Setting of Gradient
Set different temperatures hat is programmable 0～25℃
across block width (12wells) for both the left and right sides of the block.
From PCR Experiment Editor
Check a checkbox of Gradient, Enter a
temperature on the 'Temp Left' and 'Temp
Right' each other.
The predicted well temperature can be
shown by clicking the
View Gradient Temps link.
Done
From the Wizard
Appear the left temp.-right temp. of Gradient Predicted Well Temperature is refer from the left to
mode on the temperature box. right zone when looking at the machine.
✔Check
From the Wizard
Check a Gradient checkbox, Enter
a temperature on the 'Temp Left'
and 'Temp Right' each other.
Appear the left and right terminal of gradient
temperature like L70°R45°on the measuring
monitor.

Dice Touch Quick Guide -10
TAKARA BIO INC.
２．Setting of Touchdown
The means of prevent non-specific
amplification from PCR reaction.
Check the 'Touchdown' checkbox.
Given step within a cycling event to be
progressively decreased or (increased)
from an initial value to a final value
over a defined number of steps.
３．Setting of Long Range
The use of long chain length amplification
for PCR reaction.
Check the 'Long Range' checkbox.
Given step within a cycling event to be
progressively increased or (decreased)
from an initial value to a final value
over a defined number of steps.

Dice Touch Quick Guide -11
TAKARA BIO INC.
６．Manual Incubation
Touch a 'Manual Incubation' from Home screen.
Touch a column of Temp, Enter target incubate temperature then click run.
Top heat :
Check a checkbox of Top heat on the
Run control.
Top heater is warm up (105℃) with
regulated block temperature.
Time hold:
Show the Enter time screen that enter time(maximum 23:59:59) holding target
temperature.
It appear the message when
pass the setting hold time.
Gradient:
Set different temperatures hat is programmable 0～25℃across block width (12wells)
for the left and right sides of the block.

Dice Touch Quick Guide -12
TAKARA BIO INC.
７．Optional setting
Touch a 'Configuration' from Home screen.
１． System Information
２． Machine Settings
３． Time & Date
４． Thermal Calibration
５． User Accounts
６． Software Upgrade
７． Touchpanel Calibration
１． System Information
Confirm the situation of the unit.
・Serial Number
・Controller Software Version
・Firmware Version
Allows changes to be made to he instrument
２． Machine Settings settimgs.
Use predicted liquid temperature for hold times：
Using predicted temperature effectively increase
the hold times and total run time. [Default：✔]
(Based on a liquid volume is 10～50μl)
Accelerated Thermal Equilibration：[Defalt：None]
Selecting this option will use block overshoot and undershoot control to accelerate thermal equilibration.
(Optimized for reaction volumes 20～30μl)
Auto Resume Run After Power Outage：[Default:✔]
Cause the instrument to automatically resume a run from the point where a power inrerruption occurs.
Rate limit heating cooling rates：[Defalt：None]
Set rate limit for a run. This has a limit of 0.1～2,5[Max]℃. (Only available in runs not manual mode)
Use Top Heater：[Default:✔]
Cause the top heater to be used during a run if it is checked. Entry box for top heater temperature.
Temperature between 30℃～115℃ （Default 105℃）. 'Turn on Top heater NOW' button for manually
enabling the top heater to preheat it if required. (Enable top heater when set temperature >30℃)
Used to set the time for this instruments
３． Time & Date internal clock.
Set the day and time using a caｌender thaｔappear to click small arrow near the year and month.
Audible alarm on run completion：[Default:
Alert an operator that run has completed.
①②③④
⑤⑥⑦

Dice Touch Quick Guide -13
TAKARA BIO INC.
Used to adjust the machine calibration.
４． Thermal Calibration This machine is factory calibrated and should
not require user recalibration of the machine.
Click a 'Ramp Rates' button on the below column of the Calibration Wizard. Appear the
ramp rates of the temperature curve just before the reaction.
Administer the User accounts. （Refer to p.2）
５． User Accounts existing user or create or delete a user profile.
６． Software Update Allows upgrading of the operating software.
Backup the program files on internal drive
Update Software :
More information with a download file and
reference is available at the homepage.
Exit Software :
Transfer WIndowsCE to touch a button of
Exit to shell' and input password.
Backup Internal Memory
Set the USB memory stick on the device
Export Backup to USB
Files on internal drive ⇒USB memory
Import Backup to USB
Files on USB memory ⇒Internal drive
Show the folder named 'Backup-Nxxxx(Serial Number) that exist the backup files.
７．Touchpanel calibration Perform a calibration of the touch-screen
interface using the calibration wizard.
If the calibration has varied click on this button and follow
the instructions to re-calibrate the screen.
In the user area you are able to select an

TAKARA BIO INC
.
List of supplies（Tubes and Plates）
※High recovery that the sample is not hard to remain on the tube
【Attention】Please be aware that recommended caps must be used for each tube and plate.
Use of other caps or seal might decrease reaction volume by vaporization.
Nojihigashi 7-4-38, Kusatsu, Shiga, 525-0058
0.2 ml ８-Tube＆8-Cap
96 well snap plate (12×8, 96well Plate）
Flat cap for snap plate (8-Cap for Plate）
0.2ml Hi-8-Tube (8-Tube）
0.2ml Hi-8-Flat Cap (8-Cap）
0.2 ml Plate & Cap
0.2 ml Single Tube
0.2ml Hi-Tube Dome Cap Recovery ※
0.2ml Hi-Tube Flat Cap Recovery ※
NOTICE
L18 Gradient Thermocycler
This product is licensed under U.S. Patent Nos. 5,525,300; 5,779,981; 6,054,263; 6,962,821, and corresponding foreign
counterparts thereto.
V20150106