TAN Bead Maelstrom 4800 LH User manual

"TANBead” Nucleic Acid Extractor
(Non-Sterile)
Maelstrom 4800 LH
User Manual

1
Table of Contents
1.
Introduction........................................................................... 3
2.
Overview ................................................................................ 7
3.
Installation ........................................................................... 10
4.
Get started ........................................................................... 10
5.
Edit program ....................................................................... 13
6.
Communication ...................................................................20
7.
Product Performance…………………………………………….23
8.
Cleaning and maintenance ............................................... 25
9. Disposal ........................................................................... 25
10.
Patent .................................................................................. 26
11.
Appendix .............................................................................26
Revised: V1.0_202201

2
About this manual
The label on the instrument, the User Manual, and other packaging material
may contain the following symbols:
The CE mark
In Vitro Diagnostic Medical Device
Authorized European Representative
Caution, consult accompanying documents
Hot surface, risk of burns
Watch your fingers and your hands
The symbol indicates that the product should not be discarded as
unsorted waste but must sent to separate collection facilities for
recovery and recycling
Catalog number
Serial number
Manufacturer
The date of manufacture
Non-Sterile
Temp Limit
(Operating)
Biologic Risks
Keep Dry
Keep Away From Sunlight
Consult Instructions for Use
Humidity Limit
(Operating)

3
1.
Introduction
Maelstrom 4800 LH is an automated nucleic acid platform designed for high-
throughput applications. Specialized spin tips enable super mixing efficiency
of magnetic beads and larger processing volume. With intuitive interface and
flexible program, Maelstrom 4800 LH can boost laboratory productivity by
transforming routine operations into a walk-away solution.
Operation Principle
Maelstrom 4800 LH uses magnetic rods to collect and transfer magnetic
beads and spin tips to mix suspension. Rotating the spin tips will mix
suspension. Extended magnetic rods can collect magnetic beads efficiently.
Purified nucleic acids can be obtained after lysis, nucleic acids absorption,
wash and elution.
■ Flow chart for viral DNA/DNA extraction procedure
Intended Use:

Maelstrom 4800 LH can be used in combination with TANBead Nucleic Acid
Extraction Kit to extract DNA/RNA from various human specimen such as whole
blood plasma or serum.

Maelstrom 4800 LH can handle magnetic beads for purification of nucleic acid.

4
Environmental Requirements
To avoid shortening of the lifespan of the instrument, use Maelstrom 4800 LH
in a location that meets the following criteria:

Choose a location that has good air circulation.

Place Maelstrom 4800 LH on the table that can bear the weight of
Maelstrom 4800 LH (26 kg).

Do not use Maelstrom 4800 LH in a location where there is large
temperature variability or high humidity.

Operation condition:
Temperature: 4-40℃
Humidity: 40-80%

Storage and transportation condition:
Temperature: 8-40℃
Humidity: 0-85%

Maximum operating altitude: 2000 m

5
Safety instructions and guidelines

This device can be used with potentially biohazardous materials. Use
appropriate personal protective equipment (gloves, safety goggles, lab
coat, etc.) for handling and disposing of biohazardous materials.

Under normal condition, sound pressure level from Maelstrom 4800 LH
does not exceed 80 dB and cause a hazard. Please contact technical
support in case of higher sound pressure level.

This device can be hazardous due to the use of chemical and
biohazardous substances.

Users should adhere to their institutional guidelines for the handling and
disposal of all infectious substances used with this device.

It is important to clean the device after every use. If samples or reagents
have been spilled, it is important to clean the instrument immediately to
avoid damage or contamination of samples.

This device is to use with the compatible spin tips. Using incompatible
spin tips may cause poor extraction performance.

Read this user manual in its entirety prior to operating the device. Failure
to read, understand, and follow the instructions in the manual may result
in damage to the device, injury to laboratory and operating personnel or
poor performance.

6
Equipment list
No.
Item
1 Power cord
2
A plate of spin tips
3 Magnetic separator
4 User Manual

7
2.
Overview

Specifications
Model: Maelstrom 4800 LH
Weight: 26 kg
Dimensions: 29 x 55 x 28.5 cm
Controller dimensions: 32 x 30 x 14 cm
Power supply: 100-240 V
Capacity: 48 samples per run
Process. volume: 50 μl – 1600 μl
Spin speed:
500 - 3000 rpm
Heater: 2 heated plates
Magnetic rod: 3500 gauss
Display: 240 64 Dots TFT LCD

Back view of controller:

8

Side view of extraction platform:

Shield plate:

9

Regulators of the sensors for detecting falling spin tip:

Control panel:

Buttons on control panel:

10
3.
Installation
1.
Controller and extraction platform are to be connected by 25-pin power
cable and 25-pin signal line connectors.
2.
Connect electrical inlet of controller to an electrical outlet.
4. Get started
Turn on the main power switch (located on the back of controller) to the (on)
position. Once Maelstrom 4800LH is switched on, the instrument will perform a
self-test and calibration. After booting, you will see a menu showing the list of
programs in the controller:
The following example operation procedure will help you understand:
Select
the
The
Display
of
the
Controller

11
Set the
Temperature
UV LAMP
Confirm the
Selected
Program
Mount the
Spin Tips

12
Eject the
Spin Tips
STATUS:
RUNNING
STATUS:
PAUSE
STATUS:
PAUSE ?
STATUS:
END

13
5. Edit program
Programs (protocol) can be edited in PC and be transferred to the controller via
AutoMag program.
5.1
Connection
(1) Switch on Maelstrom 4800 LH.
(2) Connect (A) of transmission cable to the Maelstrom 4800 LH controller.
(Fig 2)
(3) Connect (B) to the USB port of PC. (Fig 3)
Fig 1: RS232 transmission cable
Fig 2: RS232 port of Maelstrom 4800 LH Fig 3: USB port of PC

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5.2
To start AutoMag software
(A)
Enter letters as User name. (maximum characters: 10)
(B)
Enter “0” as a default Password. (maximum characters: 8)
(C)
COM port: In drop-down menu, select the used USB port.
NOTE: The used USB port can be checked in window system control
/device manage/port.
(D)
Controller version: In drop-down menu, select “2D_M16” and click
Enter button to start AutoMag software.

15
5.3
Edit a program by AutoMag software
Parameter Name Function
Program No
File name (00~99)
Program Name
Program name (maximum characters: 15)
Plate
Plate position (1-8, 0 is program end.)
Tip
Mount/Eject spin tips (ON=Mount, OFF=Eject)
Pre-Heat
Warm up temperature (0-150℃)
Heating Execute program temperature (0-150℃)
Mixing(M) Spin time (0-999 minutes)
Speed(RPM) Spin speed (500-3000 RPM)
Shuttle Spin at the lower and the upper alternately.
Collect(s)
Collect magnetic beads (0-999 second)
Volume
Buffer volume (50-1600μl)
Vapor(M) Stand for alcohol vaporization (0-999 minutes)
Pause
Pause and wait to restart by user for adding
buffer/ enzyme or change reaction plate.

16

NOTE 1:

NOTE 2:
Definition of plate number

17
Sample programs:
(1)
SHORTTEST
STEP1: The extraction head will mount spin tips on the left half part of a spin
tip rack. After mounting the spin tips, the user can replace the spin tip rack with
a reagent deep well plate. After placement of reagent deep well plate, press
“Pause” to continue.
STEP2: The 4800 LH will perform mixing for 0.1 minutes and collecting for 10
seconds in plate 1.
STEP3: The 4800 LH will perform mixing for 0.1 minutes and collecting for 10
seconds in plate 2. After that, it will move out the plate holder and beep (Pause:
ON setting). The user can place the next reagent deep well plate and press
“Pause” button to continue.
STEP4: The 4800 LH will perform mixing for 0.1 minutes and collecting for 5
seconds in plate 3.
STEP5: The 4800 LH will perform mixing for 0.1 minutes and collecting for 10
seconds in plate 4. After that, it will move out the plate holder and beep (Pause:
ON setting). The user can place the next reagent deep well plate and press
“Pause” button to continue.
STEP6: The 4800 LH will perform mixing for 0.1 minutes and ejection of spin
tips in the left half part of reagent deep well plate.
(2)
EJECT-TIP
STEP1: The extraction head will mount spin tips from the left half part of spin
tip rack. After mounting of spin tips, it will move out plate holder. The user can
take away the spin tip rack and place an empty deep well plate. After that, press
“Pause” button to continue.
STEP2: The 4800 LH will eject spin tips in the left half part of deep well plate.

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5.4
Instructions of keys
File:
New: Create a new file
Open: Open an old file
Save: Save a file
End: Close the file
Edit:
Cut: You can cut a whole row of data when you
select a certain row. You can also cut data of
selected cells.
Copy: Copy data of a whole row
Paste: Paste copied data
Move up: Move up a whole row of data
Move
down: Move down a whole row of data
Insert:
Insert a row of blank space
Delete: You can delete a whole row of data when
you select a certain row. You can also delete data
of selected cells.
COM Port：
Choose a COM port. There 16 COM ports.
COM1 is a preset port.
Assign a new COM port with the drop-down menu.

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5.5
Transmission of programs between the PC and M4800 LH
controller

During program transmission, please do not switch off the machine and
the PC.


Import: Transfer the program from the machine to the PC (A).

Export: Transfer the programs from the PC to the machine (B).
(A)
Example of program transmission (import):
There are 3 programs installed in the controller. You can use “AutoMag” to
retrieve these programs.
-
Type “0” and press import button to get the program #0 (SHORTTEST)
from the controller. The details of the program will appear if the connection
between the PC and the controller is established.
-
Type “1” and press import button to get the program #1 (EJECT-TIP) from
the controller.