Thermo Scientific VSTAR-ISE User manual

Thermo Scientific Orion VERSA STAR™
pH/ISE/Temperature Module
Reference Guide

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Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module
Table of Contents
Chapter 1 Introduction ..........................................................1
Chapter 2 Module Overview .....................................................3
Module Connections and Inputs ........................................ 3
Module Maintenance.................................................. 4
Display Information From Module ....................................... 4
Chapter 3 Setup Menus .........................................................5
Setup Menus for Primary Measurements ................................ 5
Read Type Information..............................6
Isopotential Value..................................7
Linear Regression Information ........................7
Low Level Stability .................................7
Setup Menu for Temperature ........................................... 7
Chapter 4 pH, RmV and ORP Calibration ...........................................9
pH Calibration ........................................................ 9
RmV Calibration....................................................... 9
ORP Calibration...................................................... 10
Chapter 5 ISE Calibration.......................................................11
Chapter 6 Temperature Calibration ..............................................13
Chapter 7 Measurement........................................................15
pH/mV/RmV/ORP Measurement........................................ 15
ISE Measurement .................................................... 15
Chapter 8 Methods ............................................................17
Chapter 9 Incremental Techniques ..............................................19
Known Addition and Subtraction ...................................... 19
Single Known Addition.............................20
Double Known Addition ............................20
Single Known Subtraction ..........................21
Double Known Subtraction .........................21
Analyte Addition and Subtraction...................................... 22
To Access Incremental Techniques .................................... 22

Chapter 10 Data Storage and Review .............................................23
Data Storage Settings ................................................ 23
Measurement Read Type...........................23
Datalog, Computer and Printer Export Settings ..........23
Datalog Review ...................................................... 23
Calibration Review................................................... 24
Chapter 11 Customer Services ...................................................25
Troubleshooting Guide................................................ 25
Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Warranty and Registration ............................................ 25
WEEE Compliance ................................................... 25
Declaration of Conformity ............................................. 26
pH/ISE/Temperature Module Specifications ............................ 27
Ordering Information ................................................. 28
Appendix Advanced Features...................................................33
Automatic pH Buffer Recognition Feature............................... 33
Testing a pH Electrode for Automatic Buffer Recognition . 33
pH Electrode Condition Icon .......................................... 34
Multipoint Calibration Curve Feature .................................. 35
Automatic Blank (Non-Linear) Correction Feature ....................... 36
Determining an Isopotential Value ..................................... 37

1
Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 1 Introduction
Thank you for your purchase of the Thermo Scientific Orion VERSA STAR™ pH/ISE/Temperature Module.
This module is for use with the Thermo Scientific Orion VERSA STAR Advanced Electrochemistry Benchtop Meter.
Some key features of this module include:
• pHrangefrom-2to20withresolutionoptionsof0.001,0.01and0.1
• ISErangefrom0to19999withresolutionofuptofoursignicantdigits
• mVandRmVrangesfrom±2000mV
• Automaticandmanualdataloggingoptions
• Upto6-pointpHcalibrationwithautomaticrecognitionforUSA/NISTandDINbuffers
• Upto6-pointISEcalibrationwithadjustableISOpointcapabilities
• TheabilitytoxpH/ISEcalibrationerrorswithoutacompleterecalibrationthankstocalibrationediting
• AdvancedISEfeaturessuchasauto-blank,timedendpoint,linearpoint-to-point,non-linearselectable
auto-blank,low-concentrationrangestability,ISEIDandincrementaltechniques
Pleasereadthisreferenceguidethoroughly.Anyuseoutsideoftheseinstructionsmayinvalidateyourwarrantyand
causepermanentdamagetothemeter.

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3
Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 2 Module Overview
Toconnectthemodulestothemeterandforspecicinformationregardingthemeteroutputs,pleaseseethe
OrionVERSASTARReferenceGuide.TheReferenceGuideisontheCDincludedwiththemeterandalsoavailable
at www.thermoscientific.com/water.
Module Connections and Inputs
TheOrionVERSASTARmetercanacceptOrioncombinationISEelectrodesaswellasseparatereferenceandsensing
half-cellelectrodes.ThisallowsforawiderISEmeasurementoffering.Half-cellelectrodesseparatetheionssensing
fromthereferenceelectrode.Fordifcultsamples,theexibilityofusingalternatellsolutionsmaybeanadvantage
to researchers.
1. PreparethepHorISEelectrodeandATCprobeforuseasinstructedintheelectrodeuserguides.
The pin tip reference connector is on the lowest panel of the pH/ISE module.
2. PlacethepHorISE,ATCprobeandOrionStarstirrerprobe(cat.no.096019,optional)intothe
electrode arm.
3. AttachthepHorISEBNCandATCprobetothepH/ISEmetermodule.LowerISEandATCinto
solutionforconditioningifrequired.Ifusingthestirrerprobe,attachprobetothemeter,placeintothe
electrodearmandassigntheprobetothechannelinthesetupmenuforinstrumentsettings.
4. Preparefreshstandardsdailyforcalibration.ForISEtesting,standardsshouldbeatleastonedecade
(10times)apart.Thestandardsshouldexceedtheexpectedminimumandmaximumsamplerange.
Serialdilutionisrecommendedusingcalibratedpipettestoaccuratelypreparecalibrationstandards
from a stock solution.
5. Gatherreagents,rinsewater,andothersupplies.
 ForISEtesting,ifISAwillbeaddedtothesample,itneedstobeaddedtoallthestandardspriorto
calibrationtoensureaconsistentdilutionfactor.
pH/ISE Module
8pinmini-DINforATC
BNCconnectionwithshortingcap
Reference pin socket

4Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Module Maintenance
Forroutinemaintenance,dustandwipethemodulewithadampcloth.Ifnecessary,warmwaterormildwater-based
detergentcanbeused.Maintenancecanbeperformedonadaily,weeklyormonthlybasis,asrequiredbyoperating
environment.Immediatelyremoveanyspilledsubstanceusingthepropercleaningprocedureforthatspilltype.
Display Information from Module
ExampleISEMeasurementScreen–SingleChannel
Dateandtime
Primaryreading
Calibration
information
Corresponding
mV value
Method
Theinformationonthedisplaycanbecustomized.
Enterthesetupmenutomakechanges.
1.PressSetup.
2.UsethearrowkeystohighlightInst.Settings,
press (f3/select) key.
3.UsethearrowkeytohighlightDisplayView.
Press (f3/select) key.
4. Follow the onscreen prompts to choose what you
would like to see on the measurement screen.

5
Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 3 Setup Menus
Setup Menus for Primary Measurements
1. PressSetup (f3) to access the Main Setup Screen.
2. UsethearrowkeytoscrollacrosstothemodulestoISEchannelyouwishtouseand
press Select (f3).
3. Scroll to across to the mode menu and press Select (f3).
ISE Setup Menu
(Default values are in bold in the table below)
ElectrodeSerialNumber Input
Electrode Type Seetablebelow
SampleID Off,Manual,AutoIncrement
SignicantDigits 1,2,3,4digits
Stability Smart,Fast,Medium,Slow
Averaging Off,Automatic Smart
Read Type AutoRead,Timed,Continuous
MeasureUnit ppm,Molar,mg/L,%,ppb,None
Isopotential Value Off,On
LinearRegression Off,On
Blank Correction Off,On
LowLevelStability Off,On
Alarm** Limit,CalDue,SetPoint
**Limitandsetpointalarmdefaultsetto‘off’.
Calduealarmsetto12hours.
pH Setup Menu
(Default values are in bold in the table below)
ElectrodeID Input
SampleID Off,Manual,AutoIncrement
Resolution 0.1,0.01,0.001
Stability Smart,Fast,Medium,Slow
Averaging Off,Automatic Smart
Read Type AutoRead,Timed,Continuous
Buffer Group USA,DIN,Custom
Isopotential Value Off,On 7.000
LinearRegression Off,On
Alarm** Limit,CalDue,SetPoint
**Limitandsetpointalarmdefaultsetto‘off’.
Calduealarmsetto12hours.

6Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
4. ForISEtesting,selecttheISEelectrodebeingusedinsetup.Ifyourelectrodeisnotlisted,
usethe“X”electrodewiththevalence(+1,+2,-1,or-2)matchingyourelectrodefor
accurate results.
Read Type Information
Therearethreemainreadtypesavailableasshowninthesetupmenus.
• Auto-Read – Themeterwilldisplaythemeasurementasitstabilizesandlockandholdthe
measurementwhenitisstable(TheARiconwilllockonthescreen.)Oncethemeasurementislocked,
themeterwillautomaticallyexportthemeasurementtothedatalog,ifthedatalogisenabledin
thesetupmenu,andtoaprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterand
enabledinthesetupmenu.Totakeanewmeasurement,pressmeasure (esc)/1.
• Timed – Themeterwillreadanddisplayameasurementatthesettimeintervalthatisprogrammed
bytheoperator.Therearetwooptions:
 1. Bychecking/selectingthe"Interval"box,atthesettimeinterval,themeterwillautomatically
 exportthemeasurementtothedatalog,ifthedatalogisenabledinthesetupmenu,andto
 aprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterandenabledinthe
 setupmenu.Ifselected,enterthetimeintervalvalueinahours:minutes:secondsformatusing
the numeric keypad and press f3 (select) key.
 2. Ifthe"Interval"boxisunchecked/notselected,asingletimedmeasurementwillbetaken.
 Themeterwillapplythesamesavingandexportingconditionsaswiththesettimeintervalto
 recordandexportdata,butlockafteronetimedintervalhasexpired.Pressmeasure (esc)/1 to
 resetthecountdownclockandtakeanewreading.
ISE Types
Ag+ I- X- CO2REDOX(ORP) Cl2S-2 ClO4O2
Cd+2 NH3BF-4 K+ X-2 Cu+2 X+F- SCN-
CN- Pb+2 Cl- NO3- Br- NOxCa+2 Na+ X+2
ORP, RmV and mV Setup Menus
(Default values are in bold in the table below)
ElectrodeID Input
SampleID Off,Manual,AutoIncrement
Resolution 1.0,0.1
Stability Smart,Fast,Medium,Slow
Averaging Off,Automatic Smart
Read Type AutoRead,Timed,Continuous
Alarm** Limit,CalDue*,SetPoint
*Only for ORP and RmV modes.
**Limitandsetpointalarmdefaultsetto‘off’.
Calduealarmsetto12hours.

7
Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
• Continuous – The meter will continuously measure and update the display. This read type is useful
whenperforminganexperimentthatrequirescontinuousmeasurementstobetaken.Pressthe
log/print/0printkeytoexportthemeasurementtothedatalog,ifthedatalogisenabledinthesetup
menu,andtoaprinterorcomputer,ifaprinterorcomputerisconnectedtothemeterandenabledin
the setup.
Isopotential Value
Theslopeofallelectrodeschangeswithtemperature,basedontheNerstequation.Theslopecurvesatthedifferent
temperaturesintersectattheisopotentialvalue,whichistheconcentrationatwhichthepotentialoftheelectrodedoes
notvarywithtemperature.ForglasspHelectrodes,thisvalueis7.00.Forotherionselectiveelectrodes(ISEs),ifthe
isopotentialvalueisknownorcanbemeasuredexperimentally,adjustingthisvaluemakestemperaturecompensation
foranISEpossible.Temperaturecompensationcanbeperformedforoneandtwopointcalibrations.
Linear Regression Information
Thedefaultisoffwhichusesthetraditional,segmentedapproach.Thesegmentedapproachisthemostaccurateand
precise.MoreinformationcanbefoundintheappendixsectionofthisreferenceguideundertheMultipointCalibration
Curve Feature.
Selecting“on”forthisoptionresultsintheuseofasinglebesttlineforthecalibrationcurve,andcanbebenecialif
pHmeasurementsvarygreatly.
Low Level Stability
ThisfeatureadjuststhetimingforthecalibrationpointsforlowerlevelISEstandards.
Setup Menu for Temperature
AllofthetemperaturemeasurementsettingsarefoundinTemperatureSetupmenuforeachmeasurement.
*Thisnumberwillchangetomatchthesetupmenuchannel.
Temperatureprobescanbesetupinthemeasurementmodeofthesampleyouaremeasuring.Ch-1ATCallowsthe
temperatureprobetobeusedinmorethanonechannelfortemperature-compensatedmeasurements,suchaswhen
apHelectrodeanda2-cellconductivitycellareinthesamemeasurementsample.TemperatureisacriticalpHand
conductivitymeasurementasthemeasurementvaluesrequireatemperaturecorrectiontodeterminethereference
value,often25ºCintheAmericaand20ºCinEurope.
Temperature Unit ºCorºFselection
Temperature Input and Type Ch-1ATC,Ch-2ATC,Ch-3ATC,Ch-4ATC,MTC
Temperature Cal Ch-1*ATConlyorAllATCallowsallATCstobe
calibratedatonce

8Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
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9
Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 4 pH, RmV and ORP Calibration
pH Calibration
UptosixpHbufferscanbeusedforcalibration.Thiscanbeacombinationofautomatically-recognizedUSA/NISTor
DINbuffervaluesandcustombuffers.AlwaysusefreshpHbuffersandselectbuffersthatbracketthesamplepHand
areatleastonepHunitapart.PreparethepHelectrodeaccordingtotheinstructionsintheelectrodeuserguide.
1. Pressf1 (Cal)toperformapHcalibration.
2. Followscreenpromptsforcalibration.Usefreshbuffers,rinseelectrodewithDIwaterbeforeeach
reading,blotwithalintfreetissue.
3. USAandDINbuffersetsareautomaticallyrecognizedbasedofftheselectioninthesetupmenu,
andthemeterallowscustombufferstobeusedforupto6calibrationpoints.Custombufferoption
prompts for the nominal values and stores data.
4. Duringcalibration,pointscanbeeditedorretestedpriortoacceptance.
5. Informationforthelastcalibrationcanbedisplayedonthemainmeasurementscreen.Refertothis
referenceguide,Chapter2ModuleOverview,DisplayInformationsectionformoreinformation.
RmV Calibration
OnestandardcanbeusedforrelativemVcalibration.Alwaysusefreshstandard.Preparetheelectrodeaccordingto
theinstructionsintheelectrodeuserguide.
1. Pressf1 (Cal) toentercalibrationmode.
2. Followthescreenpromptsforcalibration.
3. Thecalibrationcanbesavedorprintedwhencomplete.
4. Informationforthelastcalibrationcanbedisplayedonthemainmeasurementscreen.Refertothis
referenceguide,Chapter2ModuleOverview,DisplayInformationsectionformoreinformation.

10 Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
ORP Calibration
ThemVreadingofanORPstandardisdependentontheORPelectrodereferencesystem,referencematerial,lling
solutionandORPstandardtemperature.AnORPelectrodecanbeautomaticallycalibratedtoread420mVrelativeto
theStandardHydrogenElectrode(EH)at25°CwhentheOrionORPstandard(cat.no.967901or96791),OrionORP
electrode(cat.no.9678BNWP,9778BNWP,9180BNMDor9179BNMD)andOrion4MKClllingsolution(cat.no.
900011)or4MKClgel(inelectrodewithcat.no.9179BNMD)areused.TherawmVreadingofanelectrodewiththe
sameconditionsis220mVat25°C.
OnestandardcanbeusedforORPcalibration.Alwaysusefreshstandard.Preparetheelectrodeaccordingtothe
instructionsintheelectrodeuserguide.
1. Pressf1 (Cal)toentercalibrationmode.
2. Followthescreenpromptsforcalibration.
3. Thecalibrationcanbesavedorprintedwhencomplete.
4. Informationforthelastcalibrationcanbedisplayedonthemainmeasurementscreen.Refertothis
referenceguide,Chapter2ModuleOverview,DisplayInformationsectionformoreinformation.

11
Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 5 ISE Calibration
OnetosixstandardscanbeusedforISEcalibration.Ifmorethanonestandardisused,startwiththelowest
concentrationrst.Alwaysusefreshstandards.Selectstandardsthatbracketthesampleconcentrationandarea
decadeapart.Preparetheionselectiveelectrodeaccordingtotheelectrodeuseguide.
1. Presschannel key until ISE channel is displayed. Press Cal (f1).Followthescreenpromptsstarting
with the lowest concentration.
2. PressEdit (f3)tothecorrectconcentrationvalueofastandardifitisnotautomaticallyrecognized.
3. Aftertherstdatapointhasbeenaccepted,pressAccept (2) tocontinuecalibration.
The meter then offers options of Esc (f1)toleavecalibration,Next (f2)measurenextcalibration
point,orCal Done (f3).
4. TheoptionsafterCalDoneispressedarePrint (f1),Cal Edit (f2) or Slope Edit (f3).
5. TheCalibrationreporttableisdisplayedwiththeconcentration,mVreading,andTemperatureand
averageslopewhichisupdatedwitheachsuccessivepointmeasured.
6. Fromthisscreenthenavigationoptionsare Print (f1),Cal Edit (f2) or Slope Edit (f3).Printexports
datatoprinterorcomputer,Caleditallowsconcentrationtobecorrectedpriortothecompletionof
thecalibrationbyDelete (f1) or ReMeasure (f3).
7. PressingtheSlopeEditchangesthescreentitletoSinglePointCal–SlopeEdit.Thisallowsthe
currentslopelinetobeupdatedwiththepresentelectrodeperformance.
8. Press Cal Done (f1)whencompleted,pressviewgraph,savethecalibrationinCalLog.

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Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 6 Temperature Calibration
TheATCtemperaturedisplayhasarelativeaccuracyof±0.1°C.ATCprobes(bothseparateandthosebuiltinto
electrodes)havevaryingtemperatureaccuracies,usually±0.5°Cto±2°C.Use this function only if necessary.
Sincethetemperatureoffsetcalculatedduringthecalibrationisappliedtoallfuturetemperaturemeasurements,
recalibrateifadifferentATCprobeisused.Fortemperaturecalibration,theprobeneedstobeconnectedtothemeter
andthecalibrationsolutionshouldhaveaknown,stabletemperature.ItisrecommendedthattwoNIST-traceable
thermometersbeusedtomeasureandverifysolutiontemperature.
1. Pressf3 (setup) key.
2. Usethearrowkeystohighlightthechannelthatthetemperatureprobeisconnectedto.
Press f3 (select).
3. UsethearrowkeystohighlightTemperatureandpressf3 (select).
4. UsethearrowkeystohighlighttheappropriateTemperatureCaloptionandpressf3 (select).
5. Follow the onscreen prompts.

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Thermo Scientific Orion VERSA STAR pH/ISE/Temperature Module
Chapter 7 Measurement
pH/mV/RmV/ORP Measurement
Afterasuccessfulcalibration,thesamplescanbetested.
1. Rinsetheelectrodewithdeionizedwater.Blotdrywithlint-freetissue.
2. Placeelectrode/ATCprobeinrstsampleandstirgently.
3. IfthemeterisinAutoReadmode(meterdefault)pressmeasure (esc) (1) key. If the meter is in
continuousortimedreadmode,themeterwillimmediatelystarttakingreadings.
4. IfinAutoReadmodeanddatalogisenabled(default),thereadingwillautomatically
bestoredwhenthe“AR”appears.Ifincontinuousreadmodeanddatalogisenabled,
press log/print (0) keytostoreintothemeter’smemory.
Note: Toenableordisabledatalogging,presssetupandselectInst.Settings.
ISE Measurement
1. Preparethesampleasinstructedintheelectrodeuserguide.
2. FollowdirectionscarefullyonISAandothersampleadditions.
3. Rinsetheprobe(s)andplaceintothesample.IfthemeterisinAutoReadmode(meterdefault)press
measure (esc) (1)key.Ifthemeterisincontinuousortimedreadmode,
themeterwillimmediatelystarttakingreadings.
4. IfinAutoReadmodeanddatalogisenabled(default),thereadingwillautomaticallybestoredwhen
the“AR”appears.Ifincontinuousreadmodeanddatalogisenabled,presslog/print (0) key to store
intothemeter’smemory.
Note:Toenableordisabledatalogging,presssetupandselectInst.Settings.

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