Thermo Scientific BIOShield 720 User manual

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Thermo Scientific
BIOShield™ 720
Instruction Manual
50121034-d• 07/ 2020

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Thermo Scientific 75003621 BIOShieldTM 720 i
T
Preface .......................................................................................................................................iii
Scope of Supply ........................................................................................................................iii
Precautions ...............................................................................................................................iii
Chapter 1 Rotor Specifications ..............................................................................................................1-1
Technical Data ........................................................................................................1-2
Chapter 2 Accessories .............................................................................................................................2-1
Chapter 3 AutoLock™.............................................................................................................................3-1
Rotor Installation .....................................................................................................3-2
Removing the Rotor .................................................................................................3-3
Chapter 4 Rotor Loading ..........................................................................................................................4-1
Before a Run ............................................................................................................4-2
Proper Loading .........................................................................................................4-2
Improper Loading ....................................................................................................4-3
Maximum Loading ...................................................................................................4-3
Cycle Counter ..........................................................................................................4-4
Chapter 5 Aerosol-tight Applications ...................................................................................................5-1
Basic Principles .........................................................................................................5-2
Placing O-ring ..........................................................................................................5-2
Fill Level ...................................................................................................................5-2
Checking the Aerosol-Tightness ...............................................................................5-2
Table of Contents
Chapter 6 Maintenance and Care ......................................................................................................... 6-1
Cleaning intervals .................................................................................................... 6-2
Cleaning .................................................................................................................. 6-2
Disinfection ............................................................................................................. 6-3
Decontamination ..................................................................................................... 6-4
Autoclaving .............................................................................................................. 6-5
Service of Thermo Fisher Scientific .......................................................................... 6-6
RCF-Values ...............................................................................................................A-1
Chemical Compatibility Chart ..............................................................................B-1

Thermo Scientific 75003621 BIOShieldTM 720 iii
P
Preface
Before starting to use the rotor, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it
is forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the seller’swarranty.
Scope of Supply
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Precautions
In order to ensure safe operation of the BIOShield™ 720, the following general safety regulations
must be followed:
•Do not remove the magnet at the rotor bottom.
•Do not use rotors which show any signs of corrosion and / or cracks.
•Use only with rotors which have been loaded properly.
•Never overload the rotor.
•Never run the rotor without lid.
Article Number Quantity Check
75003621 BIOShield™720 1
76003500 Rubber seal grease 1
75003786 Bolt grease 1
50121034 Instruction Manual 1

Preface
iv 75003621 BIOShieldTM 720 Thermo Scientific
•Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this
rule are commercially available glass or plastic centrifuge tubes, provided they have been approved
for the speed or the RCF value of the rotor.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Rotor installation: Check that the rotor is locked properly into place before operating the
centrifuge.
•Always balance the samples.
Maximum sample density at maximum speed: 1.2 g
cm3
-----------
This symbol refers to general hazards.
ATTENTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific 75003621 BIOShieldTM 720 1-1
1
Rotor Specifications
Contents
•“Technical Data” on page 1-2

1-2 75003621 BIOShieldTM 720 Thermo Scientific
1Rotor Specifications
Technical Data
Technical Data
Table 1-1. 230 V,50 / 60 Hz ventilated
Centrifuge Multifuge X1 Megafuge 16
Catalog # 75004210 75004230
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 65 / 65 45 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23 °C, running time
60 minutes
16 9
Aerosol-tight *
*Tested by HPA, Porton-down, UK
yes yes
Permissible temperature range autoclavable
°C
121 121
Table 1-2. 230 V,50 / 60 Hz refrigerated
Centrifuge Multifuge X1R Megafuge 16R
Catalog # 75004250 75004270
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 65 / 65 45 / 50
Aerosol-tight *yes yes
Permissible temperature range autoclavable
°C
121 121
*Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003621 BIOShieldTM 720 1-3
Table1-3.120 V,60 Hz ventilated
Centrifuge Multifuge X1 Megafuge 16
Catalog # 75004211 75004231
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 75 / 70 55 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23 °C, running time
60 minutes
16 9
Aerosol-tight *
*Tested by HPA, Porton-down, UK
yes yes
Permissible temperature range autoclavable
°C
121 121
Table1-4.120 V,60 Hz refrigerated
Centrifuge Multifuge X1R Megafuge 16R
Catalog # 75004251 75004271
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 75 / 70 55 / 50
Aerosol-tight *yes yes
Permissible temperature range autoclavable
°C
121 121
*Tested by HPA, Porton-down, UK

1-4 75003621 BIOShieldTM 720 Thermo Scientific
1Rotor Specifications
Technical Data
Table 1-5. 230 V, 50 / 60 Hz ventilated
Centrifuge Sorvall Legend X1 Sorvall ST 16
Catalog # 75004220 75004240
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 65 / 65 45 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23 °C, running time
60 minutes
16 9
Aerosol-tight *
*Tested by HPA, Porton-down, UK
yes yes
Permissible temperature range autoclavable
°C
121 121
Table 1-6. 230 V,50 / 60 Hz refrigerated
Centrifuge Sorvall Legend X1R Sorvall ST 16R
Catalog # 75004260 75004380
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 65 / 65 45 / 50
Aerosol-tight *yes yes
Permissible temperature range autoclavable
°C
121 121
*Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003621 BIOShieldTM 720 1-5
Table1-7.120 V,60 Hz ventilated
Centrifuge Sorvall Legend X1 Sorvall ST 16
Catalog # 75004221 75004241
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 75 / 70 55 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23 °C, running time
60 minutes
16 9
Aerosol-tight *
*Tested by HPA, Porton-down, UK
yes yes
Permissible temperature range autoclavable
°C
121 121
Table1-8.120 V,60 Hz refrigerated
Centrifuge Sorvall Legend X1R Sorvall ST 16R
Catalog # 75004261 75004381
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 75 / 70 55 / 50
Aerosol-tight *yes yes
Permissible temperature range autoclavable
°C
121 121
*Tested by HPA, Porton-down, UK

1Rotor Specifications
Technical Data
1-6 75003621 BIOShieldTM 720 Thermo Scientific
Table 1-9. 100 V,50 / 60 Hz ventilated
Centrifuge Sorvall Legend X1 Sorvall ST 16
Catalog # 75004223 75004243
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 75 / 70 55 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23 °C, running time
60 minutes
16 9
Aerosol-tight *
*Tested by HPA, Porton-down, UK
yes yes
Permissible temperature range autoclavable
°C
121 121
Table 1-10. 100 V,50 / 60 Hz refrigerated
Centrifuge Sorvall Legend X1R Sorvall ST 16R
Catalog # 75004263 75004283
Weight of empty rotor [kg] 5.7 5.7
Max. cycle number 66,000 66,000
Maximum permissible load [g] 4 x 470 4 x 470
Maximum speed nmax [rpm] 6,300 5,300
Maximum RCF value at nmax 7,188 5,088
Max. / min. radius [cm] 16.2 / 6.7 16.2 / 6.7
Pitch angle [°] 90 90
Accel. / braking time [s] 75 / 70 55 / 50
Aerosol-tight *yes yes
Permissible temperature range autoclavable
°C
121 121
*Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003621 BIOShieldTM 720 1-7
Table1-11.230 V,50 / 60 Hz ventilated
Centrifuge Thermo Scientific SL 16
Catalog # 75004000
Weight of empty rotor [kg] 5.7
Max. cycle number 66,000
Maximum permissible load [g] 4 x 470
Maximum speed nmax [rpm] 5,300
Maximum RCF value at nmax 5,088
Max. / min. radius [cm] 16.2 / 6.7
Pitch angle [°] 90
Accel. / braking time [s] 45 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23 °C, running time
60 minutes
9
Aerosol-tight *
*Tested by HPA, Porton-down, UK
yes
Permissible temperature range autoclavable
°C
121
Table1-12.230 V,50 / 60 Hz refrigerated
Centrifuge Thermo Scientific SL 16R
Catalog # 75004030
Weight of empty rotor [kg] 5.7
Max. cycle number 66,000
Maximum permissible load [g] 4 x 470
Maximum speed nmax [rpm] 5,300
Maximum RCF value at nmax 5,088
Max. / min. radius [cm] 16.2 / 6.7
Pitch angle [°] 90
Accel. / braking time [s] 45 / 50
Aerosol-tight *yes
Permissible temperature range autoclavable
°C
121
*Tested by HPA, Porton-down, UK

1Rotor Specifications
Technical Data
1-8 75003621 BIOShieldTM 720 Thermo Scientific
Table 1-13. 120 V,60 Hz ventilated
Centrifuge Thermo Scientific SL 16
Catalog # 75004001
Weight of empty rotor [kg] 5.7
Max. cycle number 66,000
Maximum permissible load [g] 4 x 470
Maximum speed nmax [rpm] 5,300
Maximum RCF value at nmax 5,088
Max. / min. radius [cm] 16.2 / 6.7
Pitch angle [°] 90
Accel. / braking time [s] 55 / 50
Sample heating at nmax [°C] referred to
ambient temperature of 23 °C, running time
60 minutes
9
Aerosol-tight *
*Tested by HPA, Porton-down, UK
yes
Permissible temperature range autoclavable
°C
121
Table 1-14. 120 V,60 Hz refrigerated
Centrifuge Thermo Scientific SL 16R
Catalog # 75004031
Weight of empty rotor [kg] 5.7
Max. cycle number 66,000
Maximum permissible load [g] 4 x 470
Maximum speed nmax [rpm] 5,300
Maximum RCF value at nmax 5,088
Max. / min. radius [cm] 16.2 / 6.7
Pitch angle [°] 90
Accel. / braking time [s] 55 / 50
Aerosol-tight *yes
Permissible temperature range autoclavable
°C
121
*Tested by HPA, Porton-down, UK

Thermo Scientific 75003621 BIOShieldTM 720 2-1
2
Accessories
Contents
•“Rotor data”on page 2-2
•“Accessories”on page 2-2

2Accessories
2-2 75003621 BIOShieldTM 720 Thermo Scientific
Rotor Specifications
* Max Speed varies dependin
g
on model of centrifu
g
e
RCF/Radius
Maximum
Minimum
BIOShield 720 Rotor Package
Cat. No. Description
75003621 BIOShield 720 with buckets and sealin
g
lid
Tube Ordering Information
Sealing Assembly Required: Adapters Required: Accessories Required:
Cat. No. Qty
/Pkg Description Cat. No. Qty /Pkg
No. of
Places
75003800 180 180 PP Screw Cap Bottle 12 6,300 56 x 123 Included 12 PP Sealing 75003693 2 1 - -
150 -Round Bottom Open
Top Tube - - 50 x 122 - - - 75003813 2 1 - -
76009007 100 75 Glass Bottle 10 -44 x 120 - - - 75003814 2 1 - -
76009084 100 75 PP Bottle 1 6,300 45 x 98 - - - 75003814 2 1 - -
76009095 100 75 PC Bottle 1 6,300 45 x 98 - - - 75003814 2 1 - -
334959 50 50 Nunc Conical 25 6,300 29.5 x 116 Included 25 PP Sealing 75003677 2 2 - -
50-
Round Bottom Open
Top Tube - - 34 x 120 -- - 75003816 2 2 - -
25 -Universal - -22.5 x 105 -- - 75003818 2 4 - -
25-
DIN Round Bottom
Tube - - 25 x 107 -- - 75003817 2 5
366036 15 15 Nunc Conical 50 6,300 16.5 x 121 Included 50 PP Sealing 75003678 2 6 - -
15 - Blood Collection - - 17 x 109 -- - 75003820 2 11 - -
10 - Blood Collection - - 17 x 109 -- - 75003820 2 11 - -
7- Blood Collection - - 13 x 115 -- - 75003821 2 12 - -
5- Blood Collection - - 13 x 115 -- - 75003821 2 12 - -
5- RIA or Round - - 13 x 115 -- - 75003822 2 20 - -
2- Conical Microtube - - 11 x 45 -- - 75003823 2 40 - -
1.5- Conical Microtube - - 11 x 45 -- - 75003823 2 40 - -
90
Thermo Scientific General Purpose Rotors BIOShield 720
4 x 180
56 x 123
Capacity (ml)
Tube Dimensions (mm)
BIOShield 720 Swin
g
in
g
Bucket Sealed Roto
r
6300*
Angle o
Maximum Speed (rpm)
K Factor
Net Weight (kg)
Max.
Speed
(rpm)
5627
5.7
RCF (x g)
71880
29700
Radius (cm)
Dims ØxL
(mm)
16.2
6.7
Qty.Cat. No.
Tube
Vol.
(ml)
Vol.
Fill
(ml)
Description
-
-
-
-
-
-
-
-
-
-
-

Thermo Scientific 75003621 BIOShieldTM 720 3-1
3
AutoLock™
Contents
•“Rotor Installation” on page 3-2
•“Removing the Rotor” on page 3-3

3AutoLock™
Rotor Installation
3-2 75003621 BIOShieldTM 720 Thermo Scientific
Rotor Installation
This rotor is equipped with an AutoLock™-system.
This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have
to be bolted onto the centrifuge spindle.
Proceed as follows:
1. Open the lid of the centrifuge and if necessary,remove any dust, foreign objects orresidue from
the chamber.
AutoLock™and O-ring must be clean and undamaged.
Figure 3-1. AutoLock™
2. Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle.
The rotor clicks automatically into place.
3. Check if the rotor is properly installed by lifting slightly on the handle. If the rotor can be pulled
up, then it must be reclamped to the centrifuge spindle.
CAUTION Unapproved or incorrectly combined accessories can cause serious damage to
the centrifuge.
CAUTION Do not force the rotor onto the centrifuge spindle.
If the rotor is very light, then it may benecessary to press it onto the centrifuge spindle
with a small amount of pressure.
WARNING If the rotor cannot be properly locked in place after several attempts, then the
AutoLock™ is defective and you are not permitted to operate the rotor.
Check for any damage tothe rotor, damaged rotors must not be used.
Keep the hub area clear of objects.
AutoLock™
O-ring