Thermo Scientific EVOS AMEP4947 User manual

EVOS™Onstage Incubator O2Sensor Replacement Kit
Catalog Number AMEP4947
Doc. Part No. 710399(01) Pub. No. MAN0025857 Rev. A.0
Note: For safety and biohazard guidelines, see the "Safety" appendix in the EVOS™M7000 Imaging System User Guide (Pub. No. MAN0018326)
or the EVOS™M5000 Imaging System User Guide (Pub. No. MAN0017563). Read the Safety Data Sheets (SDSs) and follow the handling
instructions. Wear appropriate protective eyewear, clothing, and gloves.
Kit contents
• O2sensor
• O‑ring
• 2‑mm Allen wrench
• Instructions
Replace the O2sensor
1. Power o the EVOS™Onstage Incubator, then unplug the power cord, the USB cable, and the 6-pin sensor data cable from the control unit of
the incubator.
2. Turn o the regulators on the gas tanks, then detach the gas lines from the control unit.
3. Use the 2‑mm Allen key to remove the 3 button head screws, then slide out the side panel.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
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2. Edit number and/or line-length, as needed.
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1
1
4. Unplug the O2sensor's connector (
1
: plugged,
2
: unplugged).
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
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2
USER GUIDE
For Research Use Only. Not for use in diagnostic procedures.
2 November 2021

5. Unscrew the O2sensor.
Note: You might need to use pliers (not included in the kit) to overcome the initial friction. If using pliers, do not apply excessive pressure to
prevent damage to the sensor.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
6. Verify that the O‑ring
1
is installed on the new O2sensor. If not, install the O‑ring as shown in the image.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
7. Screw in the new O2sensor until the O‑ring is compressed between the clear manifold block and the O2sensor.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
2EVOS™Onstage Incubator O2Sensor Replacement Kit

8. Plug in the O2sensor's connector
1
.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
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1
9. Install the side panel and secure it with the 3 button head screws.
10. Calibrate the new O2sensor.
Calibrate the O2sensor (EVOS™M7000 Imaging System)
1. Plug the power cord into the power input jack on the control unit and the wall outlet.
2. Connect the control unit and the computer with the USB cable, then plug the 6-pin sensor data cable from the environmental chamber into
the appropriate input jack on the control unit.
3. Re-attach the gas lines (Air and Nitrogen or CO2) via the PTC (push-to-click) connectors to the control unit, then turn on the valves on the gas
tanks and regulators.
4. First turn on the EVOS™Onstage Incubator, then power on the EVOS™M7000 Imaging System.
5. Start the EVOS™M7000 Software, navigate to the Settings tab
1
in the upper right of the screen, then open the Incubator menu
2
.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
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2
1Settings tab 2Incubator menu
EVOS™Onstage Incubator O2Sensor Replacement Kit 3

6. Open the Oxygen sensor4Calibrate controls
1
from inside the Incubator window.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
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2
3
4
1Calibrate controls
2Oxygen %
3Purge gas selection
4Begin Calibration button
7. Set the Oxygen %
2
to the oxygen content of the air used by the system.
Note: The default value for Oxygen % is 20.95% for normal compressed air.
8. Select the purge gas to use: Nitrogen or CO2
3
.
9. Press Begin Calibration
4
to start the calibration routine.
The calibration process takes approximately three minutes to complete. When complete, the system displays the “Calibration was
successful." message next to the Begin Calibration button.
If there was an error during calibration, the system displays the "Calibration error, please check if gas is connected." message.
10. In case of a calibration error, verify that the gas is connected, the gas valves and regulators are on, and the correct gas was selected as the
purge gas, then repeat the calibration routine.
Note: If the calibration error persists, contact Technical Support.
11. Press Done when finished.
4EVOS™Onstage Incubator O2Sensor Replacement Kit

Calibrate the O2sensor (EVOS™M5000 Imaging System)
1. Plug the power cord into the power input jack on the control unit and the wall outlet.
2. Connect the control unit and the computer with the USB cable, then plug the 6-pin sensor data cable from the environmental chamber into
the appropriate input jack on the control unit.
3. Re-attach the gas lines (Air and Nitrogen or CO2) via the PTC (push-to-click) connectors to the control unit, then turn on the valves on the gas
tanks and regulators.
4. First turn on the EVOS™Onstage Incubator, then power on the EVOS™M5000 Imaging System.
5. Start the EVOS™M5000 Software, click the Settings button
1
in the upper right of the screen, then click to open the Incubator menu
2
.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
2
3
1Settings button
2Incubator menu
3Oxygen Sensor Calibration button
6. Click Oxygen Sensor Calibration
3
in the Incubator menu to open the Incubator Oxygen Sensor Calibration window.
Common Callouts and Arrows
1. Copy-paste a callout or arrow to use in this SVG.
Note: If you need more advanced callouts or arrows
use the TechComm_Inkscape_Callout&Arrow_Libary.
3. Delete this text, this rectangle, and unused
callouts, arrows, or other SVG elements
before adding this SVG to the repository.
2. Edit number and/or line-length, as needed.
1 1
1
1
1
2
3
1Oxygen %
2Purge gas selection
3Begin Calibration button
7. Set the O2%
1
to the oxygen content of the air used by the system.
Note: The default value for O2% is 20.95% for normal compressed air.
8. Select the purge gas to use: Nitrogen or CO2
2
.
EVOS™Onstage Incubator O2Sensor Replacement Kit 5

9. Click Begin Calibration
3
to start the calibration routine.
The calibration process takes approximately three minutes to complete. When complete, the system displays the “Calibration was
successful." message under the Begin Calibration button.
If there was an error during calibration, the system displays the "Calibration error, please check if gas is connected." message.
10. In case of a calibration error, verify that the gas is connected, the gas valves and regulators are on, and the correct gas was selected as the
purge gas, then repeat the calibration routine.
Note: If the calibration error persists, contact Technical Support.
11. When finished, click Close to close the calibration window, then click Done to go back to the main instrument screen.
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www.thermofisher.com/support.
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The information in this guide is subject to change without notice.
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Revision history: Pub. No. MAN0025857
Revision Date Description
A.0 02 November 2021 New document
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©2021 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
For Research Use Only. Not for use in diagnostic procedures.
2 November 2021