Thermo Scientific 1025 Operating instructions

Model 1025/1029
Forma Anaerobic System
perating and Maintenance Manual 7011025 Rev. 18
Visit us online to register your warranty
www.thermoscientific.com/labwarranty

Thermo Scientific
MA UAL UMBER 7011025
18 40772 9/6/17 Updated Bottle info, pg 1-7 bpg
17 41193/IN-4777 10/20/16 Pressure switch from 360057 to 360284 on pg 7-1, update elec schematics ccs
16 31096/IN-4531 6/4/14 Change 1025-06-6 interior door assembly to 1900594 - pg 5-4, 7-1 ccs
15 29929/IN-4369 7/29/13 Ballast part number from 500033 to 500030, updated schematics ccs
14 27315/IN-4154 6/7/12 Updated 1029 electrical schematics (line cord inlet) ccs
13 27418/IN-4092 7/28/11 Updated schematics to current production ccs
12 25593/SI-10224 6/30/09 BBL strips 4370504 to R65055 ccs
11 24885/SI-10078 10/30/08 Updated schematics, 280005 from 290113 red LED ccs
10 24217/SI-9875 8/21/07 Added noise suppressor across inner door switch wires (update schematics) ccs
9 23746 6/22/07 Changed fluorescent ballast from 225329 to 500033 ccs
8 23862 1/24/07 Added warning that test strips must be kept moist to read properly ccs
7 19841 02/05/01 Corrected Figure 1-1, incubator and interchange labels reversed ccs
Preface
Anaerobic System i

Thermo Scientificii Anaerobic System
Preface
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION
Important Read this instruction manual. ailure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Caution Lamps, thermometers and thermoregulators contain mercury. Do not put in trash. Recycle or dispose as
hazardous waste. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Scientific makes no representations or warranties with respect to this manual.
In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to the use
of this manual.
©1994 Thermo Scientific. All rights reserved.

Thermo Scientific Anaerobic System iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo isher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. urther
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo Scientific products will be available at www.thermoscientific.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv Anaerobic System
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
1-740-373-4763 Direct
1-800-438-4851 Toll Free, U.S. and Canada
1-877-213-8051 FAX
http://www.thermofisher.com Internet Worldwide Web Home Page
[e-mail] Tech Support Email Address
Certified Service Web Pagewww.unitylabservices.com
Our staff can provide information on pricing and give you quotations. We canSales Support
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
Internet and we can be contacted through our Internet home page.
Our staff can supply technical information about proper setup, operation orService Support
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
Warranty for your Thermo Scientific products.
Whatever Thermo Scientific products you need or use, we will be happy to discuss your
applications. If you are experiencing technical problems, working together, we will help you
locate the problem and, chances are, correct it yourself...over the telephone without a service
call.
When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific (Asheville) LLC
401 Millcreek Road, Box 649
Marietta, OH 45750
International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific Anaerobic System v
Preface
Warranty Notes
Information You Should Know Before Requesting Warranty Service
•Locate the model and serial numbers. A serial tag is located on the unit itself.
• For equipment service or maintenance, or with technical or special application inquiries, contact Technical
Services at 1-800-438-4851 or 1-740-373-4763 (USA and Canada). Outside the USA, contact your local
distributor.
Repairs NOT Covered Under Warranty
• Calibration of control parameters. Nominal calibrations are performed at the factory; typically ±1°C for
temperature, ±1% for gases, and ±5% for humidity. Our service personnel can provide precise calibrations as
a billable service at your location. Calibration after a warranty repair is covered under the warranty.
• Damage resulting from use of improper quality water, chemicals or cleaning agents detrimental to
equipment materials.
• Service calls for improper installation or operating instructions. Corrections to any of the following
are billable services:
1) electrical service connection
2) tubing connections
3) gas regulators
4) gas tanks
5) unit leveling
6) room ventilation
7) adverse ambient temperature fluctuations
8) any repair external to the unit
• Damage resulting from accident, alteration, misuse, abuse, fire, flood, acts of God, or improper
installation.
• Repairs to parts or systems resulting from unauthorized unit modifications.
• Any labor costs other than that specified during the parts and labor warranty period, which may
include additional warranty on CO2sensors, blower motors, water jackets, etc.

Anaerobic System viThermo Scientific
Table of Contents
Installation and Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2
Connect the Vacuum Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Connect the Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Gas Recommendations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
To Connect the Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-4
Connection for Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Connection for Normal Operation . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Connect the oot Switch (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Installing the Gloves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Connect to Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
Install Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
Charcoal, Catalyst & Desiccant Wafers . . . . . . . . . . . . . . . . . . . . . . . 1-6
Prepare the Chamber Relief Bubbler . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Inspection Before Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
Start-Up Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
Automatic Operation of the Interchange . . . . . . . . . . . . . . . . . . . . . . 1-9
Manual Operation of the Interchange . . . . . . . . . . . . . . . . . . . . . . . 1-11
Initial Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-12
Set Incubator Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-12
Set Overtemp Safety Thermostat . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-12
Section 1

vii Anaerobic System Thermo Scientific
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Interchange Vacuum Gauge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Manual Chamber ill Knob . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Glove Chamber Relief Bubbler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Interchange Status Indicator Lights . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Inner and Outer Door Indicator Lights . . . . . . . . . . . . . . . . . . . . . . . 2-2
Start Cycle Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Transfer unction Switches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Interchange Cycle Indicator Lights . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
Glove Chamber Negative Pressure Switch . . . . . . . . . . . . . . . . . . . . . 2-4
Gas System Switch and Indicator Light . . . . . . . . . . . . . . . . . . . . . . . 2-4
Light Switch and Indicator Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Electrical oot Switch (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Vacuum Pump Outlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Catalyst Switch and Indicating Light . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
Power Switch and Indicator Light . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Dial Thermometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Variable/35°C Switch, Temp Control and Indicator Light . . . . . . . . 2-5
Temp Safety and Overtemp Indicator Light . . . . . . . . . . . . . . . . . . . 2-5
Disposable Anaerobic Indicator Strips . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Applying Preservative to Vinyl ront . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Leak Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Checking the Interchange . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Checking the Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Removing the Gloves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
Regenerating the Wafers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Replacing the Charcoal ilter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Gas Saving Hints . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
Table of Contents
Section 2
Section 3

Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Install the Add-On Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Join the Glove Chambers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Reassemble the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
35°C Setpoint Controller Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
35°C Setpoint Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Calibrate the 35°C Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Variable Setpoint Controller Check and Calibration . . . . . . . . . . . . 4-3
Replace luorescent Light Bulbs . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
Reverse Vacuum Solenoid Valve (In-House Vacuum) . . . . . . . . . . . . 4-3
Reverse Vacuum Solenoid Valve (In-House Vacuum) . . . . . . . . . . . . 4-4
Replace the Temperature Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Adjust the Pressure Switches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Adjust the Interchange Cycle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Replace Interchange Door Gaskets . . . . . . . . . . . . . . . . . . . . . . . . . . .4-6
Replace an Indicator Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Replace the Circulation an Motor . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Leak-check Anaerobic Interchange . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
Leak-check Anaerobic Glove Chamber . . . . . . . . . . . . . . . . . . . . . . . 5-2
Adjustment and Part Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Outer Door Latch Alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
Outer Door Hinge Alignment . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
Inner Door Latch Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Outer Door Switch Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
Inner Door Switch Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Interchange Leaks - Troubleshooting Chart . . . . . . . . . . . . . . . . . . . .5-5
General Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-1
Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-1
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-1
Anaerobic System viiiThermo Scientific
Table of Contents
Section 4
Section 5
Section 6
Section 8
Section 7

Anaerobic System 1-1Thermo Scientific
Section 1 Installation and Start-Up
If the Anaerobic System is to be moved by forklift, leave the unit on the
skid until it has been moved to its designated location. A cardboard box
containing the following accessories is packed with each unit:
Outlets
Interchange
Incubator
Control
Panel
Charcoal Filter, Palladium Catalyst
and Desiccant Wafers
Figure 1-1. Front View
Qty Stock o. Description
2 13054 Clear Plastic Tape (to secure gloves)
1 pr 188000 Glove Retaining Ring
1 188009 Waste Bag Holder
1 191022 Activated Charcoal Filter
1 191024 Palladium Catalyst Filter
1 191025 Desiccant Drying Filter
2 600034 375 Snapper Hose Clamp (connecting lines)
1 bx 921025 100 Clear Bags, 6” x 6” x12”
1 pr 921032 Size 9 Neoprene Gloves
1 285812 Plug, 15A
1 7285812 Instruction Sheet
1 380519 1/2” Hose x 1/2” FPT Adapter
Table 1-1. Packing List