Thermo Scientific Harris MBF-700 Setup guide

MBF-700
Refrigeration System
Installation, Operation and
Service Manual
325152H01 Rev. E July 2015
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IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result
in damage to the unit, injury to operating personnel, and poor equipment performance.
CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.
Material in this manual is for informational purposes only. The contents and the product it describes are
subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with
respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental,
arising from or related to the use of this manual.
© 2015 Thermo Fisher Scientific Inc. All rights reserved.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in
the product operation. This document is copyright protected and any reproduction of the whole or any
part of this document is strictly prohibited, except with the written authorization of Thermo Fisher
Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document
supersede all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or
error-free and assumes no responsibility and will not be liable for any errors, omissions, damage or
loss that might result from any use of this document, even if the information in the document is
followed properly.
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This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and
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Intended Use of the MBF-700 Refrigeration System
The MBF 700 described in this manual is a high performance unit which can be used for research and in
situations that directly support medical applications. When the MBF-700 is used to support a medical
application, it is an accessory to a medical device and is therefore considered as a medical device in its
own right by the regulatory body (e.g. FDA).
This product is intended for use:
• As cold storage in research use.
• As a medical device for diagnostic use (storage of samples not intended to be re-introduced
to the human body).
• As a medical device for blood component storage use and possible re-introduction to the
human body.
Registration : This medical application is considered a Class I medical device by the FDA. This product is
classified as product code – JRM, regulation number 862.2050 and is considered device class 1, 510(K)
exempt.

Table Of Contents
Table Of Contents
Specifications............................................................................1
Models ..................................................................................1
Exterior Dimensions ............................................................1
Power Requirements ............................................................ 1
LCO2 Backup System.......................................................... 1
Environmental Operating Conditions ..................................1
Refrigerant Charge............................................................... 2
Oil Charge ............................................................................2
Safety Precautions....................................................................3
Unpacking.................................................................................5
General Description.................................................................6
Construction .........................................................................6
Incoming Power Wiring .......................................................8
Installation and Operation......................................................9
Installation Checklist............................................................ 9
Responsibilities .................................................................. 10
Control Panel ......................................................................12
Loading Instructions .......................................................... 13
Options and Accessories........................................................15
Water-cooled Condensing Unit Connections..................... 15
Installing the Remote Alarm Connector ............................ 17
Liquid CO2(LCO2) Backup System....................................19
Installation.......................................................................... 19
LCO2Control System Battery Condition .......................... 20
Liquid CO2Backup Installed as Accessory #6100............ 20
Cobex 7-day Circular Chart Temperature Recorder ........24
Setup and Operation ...........................................................24
Maintenance ....................................................................... 25
Theory of Operation..............................................................33
Overview ............................................................................33
Sequence of Operation .......................................................33
Systems Description........................................................... 34
Operation............................................................................ 37
Maintenance and Service ......................................................41
Cleaning the Freezer Surfaces............................................ 41
Air Cooled Condensers ......................................................41
Compressor Oil Level ........................................................ 41
Fan Motors .........................................................................41
Defrosting ...........................................................................41

Table Of Contents
LCO2Vent .........................................................................42
Storage ...............................................................................42
Troubleshooting.....................................................................43
Failure Analysis......................................................................48
Contactors and Relays........................................................ 48
Compressor Motors, Three Phase ......................................48
Partial or Complete Loss of Refrigeration .........................48
Checking Refrigerant Charge .............................................49
Installing Gauges ................................................................49
Leak Testing, Repairing and Charging – an Overview ......50
Repair Procedures .................................................................53
Compressor Replacement ..................................................53
Backflush Procedure ..........................................................56
Evacuation Procedure ........................................................58
Charging Procedure -- 1st (High) Stage ............................. 60
Charging Procedure-- 2nd (Low) Stage.............................. 60
Temperature Calibration......................................................62
Warranty ................................................................................63
Appendix A.............................................................................65
Refrigeration System Data .................................................65
Service Parts List ............................................................... 66
Temperature Conversion Table ..........................................70
230 V Wiring Diagram ......................................................71
12 V Wiring Diagram ........................................................72
LCO2Backup System Electrical Schematic ......................73
Appendix B: Primary Controller .........................................74
Dixell Operating Manual.......................................................75
Introduction ........................................................................75
Overview ............................................................................76
Controlling Loads ..............................................................76
Front Panel Commands ......................................................79
MAX and MIN Temperature Memorization ......................81
Main Functions ..................................................................82
Parameters ..........................................................................85
Digital Inputs...................................................................... 92
RS485 Serial Line (for Monitoring Systems) ....................94
X-REP Output(Optional) ................................................... 94
Installation and Mounting ..................................................94
Electrical Connections .......................................................95
How to Use the Hot Key ....................................................95
Alarm Signals .....................................................................96
Specifications .....................................................................98
Connections ........................................................................99

Table Of Contents
E2 MODBUS Network Wiring ..........................................99
ECT MODBUS Networking to E2s................................. 101
Default Setting Values .....................................................107
Dixell Parameter Table .......................................................112

Specifications
Thermo Scientific MBF-700 Refrigeration System Installation, Operation and Service Manual 1
1 Specifications
1.1 Models The following MBF-700 models are described in this manual:
1.2 Exterior Dimensions
1.3 Power Requirements
1.4 LCO2Backup
System 115 V, 120 V, 230 V available
Phase 1
1.5 Environmental
Operating Conditions
Model Number Revision Level
MBF700LSAO-E Rev. 20
MBF700LSAW-E Rev. 20
Height 47.5 in (121 cm)
Width 121 in (307 cm)
Depth 35.3 in (90 cm)
Weight 1510 lbs (685 kg) – shipping weight 1790 lbs (812 kg)
Frequency 60 Hz 50 Hz
Voltage 208/230 220
Phase 3 3
Amperage 24 A 38 A
Pollution Degree 2
Installation Category II
Altitude 2000 m MSL (mean sea level)
Humidity max 80% - non-condensing
Voltage Tolerance ±10%

Specifications
2 Installation, Operation and Service Manual Thermo Scientific MBF-700 Refrigeration System
1.6 Refrigerant Charge
1.7 Oil Charge
Stage 1 R404a (50 oz)
Stage 2 R290 (1.5 oz), R508b (20.3 oz)
Stage 1 50 oz POE RL32H
Stage 2 65 oz Alkyl Benzene Zerol

Safety Precautions
Thermo Scientific MBF-700 Refrigeration System Installation, Operation and Service Manual 3
2 Safety Precautions In this manual, the following symbols and conventions are used:
This symbol used alone indicates important operating instructions which
reduce the risk of injury or poor performance of the unit.
CAUTION: This symbol, in the context of a CAUTION, indicates a
potentially hazardous situation which if not avoided could result in minor to
moderate injury or damage to the equipment.
WARNING: This symbol indicates situations where dangerous voltages
exist and potential for electrical shock is present.
The snowflake symbol indicates extreme low temperatures and high risk of
frostbite. Do not touch bare metal or samples with unprotected body parts.
This symbol indicates a need to use gloves during the indicated procedures.
If performing decontamination procedures, use chemically resistant gloves.
Use insulated gloves for handling samples and when using liquid nitrogen.
Before installing, using or maintaining this product, please be sure to read
this manual and product warning labels carefully. Failure to follow these
instructions may cause this product to malfunction, which could result in
injury or damage.

Safety Precautions
4 Installation, Operation and Service Manual Thermo Scientific MBF-700 Refrigeration System
Below are important safety precautions that apply to this product:
Use this product only in the way described in the product literature and in
this manual. Before using it, verify that this product is suitable for its
intended use. If this equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be impaired.
Do not modify system components, especially the controller. Use OEM
exact replacement equipment or parts. Before use, confirm that the product
has not been altered in any way.
WARNING: Your unit must be properly grounded in conformity with
national and local electrical codes. Never connect the unit to overloaded
power sources.
WARNING: Disconnect the unit from all power sources before cleaning,
troubleshooting, or performing other maintenance on the product or its
controls.

Unpacking
Thermo Scientific MBF-700 Refrigeration System Installation, Operation and Service Manual 5
3 Unpacking At delivery, examine the exterior for physical damage while the carrier’s
representative is present. If exterior damage is present, carefully unpack and
inspect the unit and all accessories for damage.
If there is no exterior damage, unpack and inspect the equipment within five
days of delivery. If you find any damage, keep the packing materials and
immediately report the damage to the carrier. Do not return goods to the
manufacturer without written authorization. When submitting a claim for
shipping damage, request that the carrier inspect the shipping container and
equipment.
CAUTION: Do not discard the sub lids. They are necessary for the
operation of the unit.

General Description
6 Installation, Operation and Service Manual Thermo Scientific MBF-700 Refrigeration System
4 General
Description This unit is a low temperature freezer designed to operate with a chamber
temperature in the range of –65 to –80 °C (-85 to –112 °F) and in an
ambient temperature range of 15 to 32 °C (59 to 89.6 °F).
Figure 1. General Freezer Outline (refer to Figure 4 for control panel
details)
The interior useable space is also rectangular. The four corners are coved.
With insulating sub lids in place it measures 71” long (180.3 cm)x 35.37”
high (89.9 cm) x 22.63” deep (57.5 cm). The interior volume is 32 Ft.(929
liters.)
The cabinet is insulated with nominal 5” of foamed-in-place polyurethane
insulation.
The refrigeration system used in this freezer is a specially designed assembly
using four hermetic motor compressors. This design is termed a “redundant
cascade system” described in detail in Section 9.
Stainless steel storage racks for inventory control are available as an option
and must be ordered separately.
4.1 Construction The lid is held tightly closed by means of cam locks having provision for a
user supplied padlock.
Durable corrosion protection is provided by an electrostatically applied
epoxy fused powder coating over cold rolled steel.

General Description
Thermo Scientific MBF-700 Refrigeration System Installation, Operation and Service Manual 7
To provide a redundant refrigeration system, the inner tank is double
wrapped with copper tubing forming a dual circuit evaporator. In addition,
each of these circuits has its own compressor, condenser and liquid
refrigerant control (capillary tube). Each of the redundant systems will
support full operation to maintain the storage temperature. When set to
alternate between system “A” and system “B”, refrigeration is applied to the
freezer chamber alternating between systems on each cycle.
Figure 2. Cabinet Dimensional Drawing

General Description
8 Installation, Operation and Service Manual Thermo Scientific MBF-700 Refrigeration System
4.2 Incoming Power
Wiring The illustration below shows the incoming power wiring and label:
Figure 3. Incoming Power Wiring
Note that the compressor is not rotation-specific. The contactor is wired as
“L L L”.
WARNING: Only a licensed electrician is to make these connections.

Installation and Operation
Thermo Scientific MBF-700 Refrigeration System Installation, Operation and Service Manual 9
5 Installation and
Operation WARNING: The MBF-700 is intended to be hard-wired upon installation.
Use with quick-disconnect plugs is not approved. The unit must be installed
with proper circuit breakers and overcurrent protection, and installation
must be done by a licensed electrician in accordance with all applicable
building codes and regulations.
Power conductors must be at least 10 AWG. Heavier wire may be required
due to the specifics of a given installation or variations in applicable
regulations. The circuit breaker must be located in close proximity to the
equipment within easy reach of the operator, and must be clearly marked as
the disconnect device for the equipment.
CAUTION: Do not attempt to operate this freezer until the following
Installation Check List steps have been completed.
Note Install with clearances of 6 inches at rear and 8 inches on sides.
5.1 Installation
Checklist
Date Initials
The freezer is in place.
The freezer has been levelled.
Check freezer data plate voltage.
Check supply voltage is the same as data plate.
Check that power panel circuit protection rating does not
exceed that indicated on the data plate.
Backup system has been installed (refer to Section 7)
and LCO2supply valve is Off.
Facility monitoring system connected to freezer’s remote
alarm contacts (refer to Section 6.2).
If unit uses a water-cooled condenser, water supply and
discharge are connected in accordance with Section 6.1.
No product loaded in freezer.
Main building power panel breakers are On.
Using key provided, turn the compressor key switch to
ON.

Installation and Operation
10 Installation, Operation and Service Manual Thermo Scientific MBF-700 Refrigeration System
5.2 Responsibilities Note The following table define the Operator's responsibilities for reporting
trouble symptoms on startup of the freezer and also observing its operation on an
ongoing basis in order to prevent any mechanical or electrical failures that could
result in loss of blood products.
Set primary control set points. (refer to Appendix B).
The frame heater switch (#8, Figure 4) is shipped from
the factory in the OFF position. Turn frame heater switch
ON only if the humidity is sufficiently high that it may
cause frost on the strips.
Turn LCO2supply valve On after cabinet reaches.
Frequency
Of Task Description Of Tasks, Observations & Record Keeping Requirements
Once on first
start
a.
Time required for initial pulldown. If only partial loss of the refrigerant has occurred, the sign will be
slow pulldown time required reaching -80 °C. Pulldown time should be 2.5 hours or less. If it is more
than 2.5 hours, the technician must either determine the cause and the remedy therefore or
IMMEDIATELY SHUT DOWN THE FREEZER until the problem can be diagnosed and remedied.
b. Carefully record the first few on-off periods with freezer empty, and then again loaded with product.
Keep this record with the freezer at all times for future reference in troubleshooting.
c.
If the installation is made in an air-conditioned area, there will not be much, if any, change in cycle.
However, if the ambient temperature is subject to changes, make a record of cycle times at these various
ambient temperatures.
Daily
d. Listen for rattles. If refrigerant-carrying copper tubing is allowed to rub against other tubes or any object,
a leak will very quickly occur resulting in loss of the refrigerant charge and cabinet temperature.
e.
Watch the system operation indicator light. It should not be showing the same system always operating.
For instance, when system A has been operating and shuts off, system B should start on the next control
call for cooling.

Installation and Operation
Thermo Scientific MBF-700 Refrigeration System Installation, Operation and Service Manual 11
Weekly
f. Air-Cooled units only: clean the condenser air filter when its original jet-black color has changed.
Attention to this detail will assure shortest running times and prolong the life of the freezer.
g. Make sure the lid closes without interference and that sub lids are always in place.
h. Ensure that the sub lids are in place.
i. Verify that the LCO2vent is free of frost accumulation.
On Using
j. Watch for lid gasket tears. Streaks of frost will collect inside the freezer at the precise location of the torn
or cut gaskets.
k. Defrost the cabinet if frost or ice build-up exceeds 1/4” thickness: remove product, turn power key switch
Off, leave unit open 48 hours, remove moisture, restart unit.

Installation and Operation
12 Installation, Operation and Service Manual Thermo Scientific MBF-700 Refrigeration System
5.3 Control Panel All controls are located at the right end of the cabinet. Refer to Appendix B
for instructions on controller operation.
Figure 4. Control Panel Detail
1. Key-operated alarm ON/OFF switch. Turns on local audio alarm.
2. Key-operated compressor ON/OFF switch. Turns on power to
compressors and control system.
3. Digital temperature indicator and primary control.
4. Temperature recorder, 7-day, 6” diameter.
5. System “B” indicator light. “ON” when system “B” 1st stage running.
6. Alternate system alarm indicator light. “ON” when auxiliary control
causing both systems to operate.
7. System “A” indicator light. “ON” when system “A” 1st stage running.
8. Frame heater switch. Operates breaker strip condensate heater.
9. Battery meter. Displays state of battery charge when button is pressed.

Installation and Operation
Thermo Scientific MBF-700 Refrigeration System Installation, Operation and Service Manual 13
5.4 Loading Instructions The MBF-700 freezer is designed to be used for long-term storage and is not
intended to be operated as a blast freezer. The unit has dual refrigeration
systems primarily to provide redundant reliability in the event of a
catastrophic failure of one side.
A secondary result of having dual refrigeration systems is that the unit also
has a greater heat removal capacity than a conventional ULT freezer. This
feature makes the MBF-700 more suitable for use with large product loads
than a typical ULT; subject to the following loading recommendations.
Overloading the freezer beyond these recommendations can result in
excessive temperatures and pressures which may be detrimental to unit life
and reliability. For applications that will exceed these restrictions it is
recommended that the user select and qualify a dedicated purpose-built
blast freezer that will be suitable for the highest anticipated load.
The general requirements that apply to all loads are as follows:
• Upon initial startup the unit must be empty. Do not place any
payload in the freezer until it is cycling at setpoint temperature.
• The maximum payload temperature is 32 °C.
• The maximum door opening time is 5 minutes.
• The load should be evenly distributed throughout the cabinet as
much as is practical. For example, it is preferable to load in
horizontal layers rather than vertical stacks.
• The unit must be permitted to cycle normally for at least one hour
after the cabinet returns to the setpoint temperature before another
load is placed inside. This ensures that the unit has made a complete
recovery and prevents successive loads from combining to create an
overload condition.
Additionally, the limits below must be followed depending on the nature of
the payload:
•Forliquid loads that will freeze solid inside the cabinet the
maximum mass of an individual load is 30 kg of liquid plus 10 kg
of solid material such as bottles or racks.
CAUTION: Water-based solutions expand on freezing. Do not use glass
containers, which may burst and present a cut or contamination hazard.
•Forsolid loads that will not change phase inside the cabinet the
maximum mass of an individual load is 50 kg total, including both
the payload and any other material such as storage containers or
racks.