ThermoFisher Scientific NanoDrop Eight User manual

NanoDrop Micro-UV/Vis Spectrophotometer
NanoDrop Eight
User Guide
M020 NanoDrop Eight UG Revision Date December 2021

© 2021 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to
use in the product operation. This document is copyright protected and any reproduction of the
whole or any part of this document is strictly prohibited, except with the written authorization of
Thermo Fisher Scientific Inc.
For U.S. Technical Support, please contact:
Thermo Fisher Scientific
3411 Silverside Road
Tatnall Building, Suite 100
Wilmington, DE 19810 U.S.A.
Telephone: [phone]
Toll Free: 1 877 724 7690 (U.S. & Canada only)
E-mail: [e-mail]
For International Support, please contact:
http://www.thermofisher.com/
NanoDropSupport
Contact your local distributor. For contact
information go to:
http://www.thermofisher.com/
NanoDropDistributors
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document
supersede all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that this document is complete, accurate or
error-free and assumes no responsibility and will not be liable for any errors, omissions, damage or
loss that might result from any use of this document, even if the information in the document is
followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale,
which Terms and Conditions of Sale shall govern all conflicting information between the two
documents.
For Research Use Only. This instrument or accessory is not a medical device and is not intended to
be used for the prevention, diagnosis, treatment or cure of disease.
WARNING Avoid an explosion or fire hazard. This instrument or accessory is not
designed for use in an explosive atmosphere.

Thermo Scientific NanoDrop Eight User Guide iii
C
Chapter 1 About the Spectrophotometer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
USB-B port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
Accessories. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
PR-1 Pedestal Reconditioning Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
PV-8 Performance Verification Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Instrument Detection Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
Chapter 2 Instrument Set up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Register Your Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Computer Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Update Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
For U.S./Canada Support, please contact: . . . . . . . . . . . . . . . . . . . .12
For International Support, please contact: . . . . . . . . . . . . . . . . . . . . .12
Chapter 3 Application Measurement Ranges . . . . . . . . . . . . . . . . . . . . . . . . . .13
Detection Limits for All Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Chapter 4 Nucleic Acid Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Measure dsDNA, ssDNA or RNA. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Measure dsDNA, ssDNA or RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Best practices for nucleic acid measurements . . . . . . . . . . . . . . . . . .17
Nucleic Acid Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Settings for Nucleic Acid Measurements . . . . . . . . . . . . . . . . . . . . . .20
Calculations for Nucleic Acid Measurements . . . . . . . . . . . . . . . . . . .21
Measure using a Custom Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Measure Nucleic Acid using a Custom Factor . . . . . . . . . . . . . . . . . .25
Custom Factor Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Settings for Nucleic Acid Measurements using a Custom Factor . . . .28
Detection Limits for Nucleic Acid Measurements using a Custom
Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Contents

Contents
iv NanoDrop Eight User Guide Thermo Scientific
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Measure Oligo DNA or Oligo RNA . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Oligo Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Settings for Oligo DNA and Oligo RNA Measurements. . . . . . . . . . . .35
Detection Limits for Oligo DNA and Oligo RNA Measurements. . . . . .36
Calculations for Oligo DNA and Oligo RNA Measurements. . . . . . . . .37
Chapter 5 Protein Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Measure Protein A280. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Measure Protein Concentration at A280 . . . . . . . . . . . . . . . . . . . . . .41
Best practices for protein measurements. . . . . . . . . . . . . . . . . . . . . .43
Protein A280 Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
Settings for Protein A280 Measurements . . . . . . . . . . . . . . . . . . . . . .46
Protein editor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
Detection Limits for Protein A280 Measurements. . . . . . . . . . . . . . . .51
Calculations for Protein A280 Measurements. . . . . . . . . . . . . . . . . . .51
Chapter 6 Custom Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Measure UV-Vis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
Measure UV-Vis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .58
Best practices for UV-Vis measurements . . . . . . . . . . . . . . . . . . . . . .59
UV-Vis Reported Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Settings for UV-Vis Measurements. . . . . . . . . . . . . . . . . . . . . . . . . . .62
Measure Custom. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
Measure using a Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . .63
Delete Custom Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64
Custom Method Reported Results. . . . . . . . . . . . . . . . . . . . . . . . . . .65
Manage Custom Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66
Chapter 7 Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
Micro-Volume Sampling—How it Works . . . . . . . . . . . . . . . . . . . . . . . .78
Set Up the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Connect Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Connect to a Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80
Operating Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Measure a Micro-Volume Sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82
Best practices for micro-volume measurements. . . . . . . . . . . . . . . . .82
Recommended sample volumes . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Sample Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Module startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Sample Plate Map . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Prepare Samples and Blanks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Preparing Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Run a Blanking Cycle. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90

Contents
Thermo Scientific NanoDrop Eight User Guide v
Basic Instrument Operations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92
NanoDrop Eight Home Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92
NanoDrop Eight Measurement Screens . . . . . . . . . . . . . . . . . . . . . . .95
Measurement Screen Display Options . . . . . . . . . . . . . . . . . . . . . . . .96
NanoDrop Eight General Operations . . . . . . . . . . . . . . . . . . . . . . . .109
Acclaro Sample Intelligence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117
Activate Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117
View Acclaro Sample Intelligence Information. . . . . . . . . . . . . . . . . .117
Contaminant Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119
On-Demand Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . .122
Instrument Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123
System Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123
Preferences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124
Protein Editor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124
Measurement Screen Display Options . . . . . . . . . . . . . . . . . . . . . . . . .124
Chapter 8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
Maintenance Schedule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128
Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128
Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128
Every 6 Months . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .128
Maintaining the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129
Cleaning the Pedestals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .129
Reconditioning the Pedestals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .131
Decontaminating the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133
Instrument Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .135
Intensity Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .136
Performance Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .138
Chapter 9 Safety and Operating Precautions . . . . . . . . . . . . . . . . . . . . . . . . .143
Operating Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .144
Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .145
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .145
When the System Arrives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .147
Lifting or Moving the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . .147
Electrical Requirements and Safety . . . . . . . . . . . . . . . . . . . . . . . . .148
Power Cords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .148
Fire Safety and Burn Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149
Optical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .150
Hazardous Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .150

Contents
vi NanoDrop Eight User Guide Thermo Scientific

Thermo Scientific NanoDrop Eight User Guide 7
1
About the Spectrophotometer
The Thermo Scientific™ NanoDrop™ Eight is a UV-Visible spectrophotometer
developed for micro-volume analysis of a wide variety of analytes. The patented
sample retention system enables the measurement of highly concentrated samples
without the need for dilutions. The instrument accurately measures up to 8 individual
1 µl samples in one measurement cycle. The software allows the user to measure
samples using either the full 8-channel mode or a convenient single sample mode.
The NanoDrop Eight software is installed on a local PC used to control the
instrument and view data.
Note Locate the instrument away from air vents and exhaust fans to minimize
evaporation
NanoDrop Eight Spectrophotometer
Arm
Pedestal
Well tray sample
indicator array

1About the Spectrophotometer
Features
8 NanoDrop Eight User Guide Thermo Scientific
Features
TheNanoDrop Eight spectrophotometer features the patented micro-volume sample
retention system.
USB-B port
Connect the NanoDrop Eight to your PC via USB to operate the instrument using the
PC software.
Note Before operating a NanoDrop Eight instrument, please read the safety and
operating precautions and then follow their recommendations when using the
instrument.

1 About the Spectrophotometer
Accessories
Thermo Scientific NanoDrop Eight User Guide 9
Accessories
This section lists the accessories included for use with the NanoDrop Eight.
PR-1 Pedestal Reconditioning Kit
PV-8 Performance Verification Kit
Kit containing plastic consumables and liquid photometric standard used to check
instrument performance. For more information, see Performance Verification.
Specially formulated conditioning compound
that can be applied to the pedestals to restore
them to a hydrophobic state (required to
achieve adequate surface tension for accurate
sample measurements). The kit includes
conditioning compound and applicators. For
more information, see Reconditioning the
Pedestals.

1About the Spectrophotometer
Instrument Detection Limits
10 NanoDrop Eight User Guide Thermo Scientific
Instrument Detection Limits
Pathlength (mm)
Upper Detection Limit
(10 mm Equivalent Absorbance)
1.0 12.5
0.2 90
0.1 200

Thermo Scientific NanoDrop Eight User Guide 11
2
Instrument Set up
Register Your Instrument
Register your instrument to receive e-mail updates on software and accessories for
the NanoDrop Eight instrument. An Internet connection is required for registration.
To register your instrument
From any PC that is connected to the Internet, use any web browser to navigate to
www.thermofisher.com/nanodropsw.
On the website, locate the Register Your Instrument button, and follow the
instructions to register the instrument.
Computer Requirements
Required Windows Operating System
• Windows 10 Enterprise or Professional, build 1607 or greater
Minimum hardware configuration
• 2.0 GHz dual-core processor enabled
• 4 GB RAM with system managed memory enabled
• 5 GB available on drive C
• Display resolution 1366 × 768
Recommended hardware configuration
• 2.33 GHz 4-core processor enabled (or greater)
• 8 GB RAM with system managed memory enabled (or greater)
• 200 GB available on drive C (or greater)

2Instrument Set up
Update Software
12 NanoDrop Eight User Guide Thermo Scientific
Update Software
Quickly and easily download and install the latest NanoDrop Eight software and
release notes from our website. Follow the steps to update or upgrade the
NanoDrop Eight software on a personal computer (PC). An Internet connection is
required to download software. Navigate to www.thermofisher.com/nanodropsw
and click on the NanoDrop Eight software tab. Follow the Instrument Software
Download instructions.
To install or update NanoDrop Eight software on a PC
1. Insert the USB flash drive containing the installer software into an available USB
port on your PC, or open the installation folder downloaded from the internet.
2. Launch Start.exe and click the Software button. The software installer will
run.
Technical Support
For U.S./Canada Support, please contact:
Thermo Fisher Scientific
3411 Silverside Road
Tatnall Building, Suite 100
Wilmington, DE 19810 U.S.A.
Telephone: [phone]
Toll Free: 1 877 724 7690 (U.S. & Canada only)
Fax: [phone]
E-mail: [e-mail]
Website: www.thermofisher.com/nanodrop
For International Support, please contact:
Contact your local distributor. For contact information go to:
http://www.thermofisher.com/NanoDropDistributors
If you are experiencing an issue with your system, refer to the troubleshooting
information. If the issue persists, contact us. If you are outside the U.S.A. and
Canada, please contact your local distributor.
If your instrument requires maintenance or repair, contact us or your local distributor.

Thermo Scientific NanoDrop Eight User Guide 13
3
Application Measurement Ranges
Detection Limits for All Applications
Note Detection limits provided in the following tables are approximate and apply
to micro-volume measurements only; they are based on the instrument’s
photometric absorbance range (10 mm equivalent) of 0.04–200 A.
Sample Type Lower Detection
Limit
Upper Detection
Limit Typical Reproducibilitya
aBased on five replicates (SD=ng/µL; CV=%)
dsDNA 2.0 ng/µL 10,000 ng/µL ±2.0 ng/µL for sample concentrations
between 2.0 and 100 ng/µL samples;
±2% for samples >100 ng/µL
RNA 1.6 ng/µL 8,000 ng/µL ±2.0 ng/µL for sample concentrations
between 2.0 and 100 ng/µL samples;
±2% for samples >100 ng/µL
Purified BSA by
Protein A280
IgG by Protein A280
0.06 mg/mL
0.03 mg/mL
300 mg/mL
145 mg/mL
±0.10 mg/mL (for 0.10–10 mg/mL
samples);
±2% for samples >10 mg/mL

3Application Measurement Ranges
14 NanoDrop Eight User Guide Thermo Scientific
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Thermo Scientific NanoDrop Eight User Guide 15
4
Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
Measure dsDNA, ssDNA or RNA
Use the dsDNA, ssDNA and RNA applications to quantify purified double-stranded
(ds) or RNA samples. These applications report nucleic acid concentration and two
absorbance ratios (A260/A280 and A260/A230). A single-point baseline correction
can also be used.
To measure dsDNA, ssDNA or RNA samples
Measures the concentration of purified
dsDNA, ssDNA or RNA samples that
absorb at 260 nm.
Measure dsDNA, ssDNA or RNA
Reported Results
Settings
Detection Limits
Calculations
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.

4Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
16 NanoDrop Eight User Guide Thermo Scientific
Before you begin...
Before taking pedestal measurements with the NanoDrop Eight instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see Cleaning the
Pedestals.
To measure nucleic acid
1. From the Home screen, select the Nucleic Acids tab, select your sample
mode, and select dsDNA, ssDNA or RNA, depending on the samples to be
measured.
2. Specify a baseline correction if desired.
3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm.
4. Select Blank and wait for the measurement to complete.
Tip: If Auto-Blank is On, the blank measurement starts automatically after you
lower the arm.
5. Lift the arm and clean all pedestals with a new laboratory wipe.
6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm.
7. Start the sample measurement: If Auto-Measure is On, lower arm;
if Auto-Measure is off, lower arm and select Measure .
When the sample measurement is completed, the spectrum and reported values
are displayed (see the next section).
8. When you are finished measuring samples, select End Experiment .
9. Lift the arm and clean all pedestals with a new wipe.

4 Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
Thermo Scientific NanoDrop Eight User Guide 17
Best practices for nucleic acid measurements
• Isolate and purify nucleic acid samples before measurement to remove
impurities. Depending on the sample, impurities could include DNA, RNA, free
nucleotides, proteins, some buffer components and dyes. See Preparing
Samples for more information.
Typical nucleic acid spectrum
Comparison of nucleic acid spectra with and without two
common contaminants

4Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
18 NanoDrop Eight User Guide Thermo Scientific
• Ensure the sample absorbance is within the instrument’s absorbance detection
limits.
• Blank with the same buffer solution used to resuspend the analyte of interest.
The blanking solution should be a similar pH and ionic strength as the analyte
solution.
• Run a blanking cycle to assess the absorbance contribution of your buffer
solution. If the buffer exhibits strong absorbance at or near the analysis
wavelength (typically 260 nm), you may need to choose a different buffer or
application. See Choosing and Measuring a Blank for more information.
• For micro-volume measurements:
– Ensure pedestal surfaces are properly cleaned and conditioned.
– If possible, heat highly concentrated or large molecule samples, such as
genomic or lambda DNA, to 63 °C (145 °F) and gently (but thoroughly) vortex
before taking a measurement. Avoid introducing bubbles when mixing and
pipetting.
– Follow best practices for micro-volume measurements.
– Use a 1-2 µL sample volume. See Recommended Sample Volumes for more
information.
Related Topics
• Measure a Micro-Volume Sample
• Best Practices for Micro-Volume Measurements
• Prepare Samples and Blanks
• Basic Instrument Operations
Note Extraction reagents such as guanidine, phenol, and EDTA contribute
absorbance between 230 nm and 280 nm and will affect measurement
results if present in samples (even residual amounts).

4 Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
Thermo Scientific NanoDrop Eight User Guide 19
Nucleic Acid Reported Results
dsDNA measurement screen
For each measured sample, the dsDNA, ssDNA and RNA applications show the UV
absorbance spectrum and a summary of the results. Below is an example of the
measurement screen of the instrument software:
Click row to select sample
and update spectrum.
UV spectrum
Right click graph
area to view display
options.
M
easure samp
l
e
End experiment
Run Blank
Purity ratios
Menu of table
options;
click to choose
which columns to
report
Nucleic acid
concentration
Sample name;
select to edit
Application
Well plate
options
Baseline
correction
Wellplate view;
toggle to view/ hide
well plate map
Load Sample ID file
View history data
Auto measure
Note Micro-volume absorbance measurements are normalized to a 10.0 mm
pathlength equivalent.

4Nucleic Acid Applications
Measure dsDNA, ssDNA or RNA
20 NanoDrop Eight User Guide Thermo Scientific
dsDNA, ssDNA and RNA reported values
• sample name
• created on (date sample measurement was taken)
• nucleic acid concentration
• Species
• A260/A280
• A260/A230
• A260
• A280
• factor
• baseline correction
• monitor wavelength
• pathlength used
• contaminant
• corrected
Settings for Nucleic Acid Measurements
Baseline Correction
For the dsDNA, ssDNA or RNA, enter baseline correction wavelength in nm or use
default value (340 nm)
Optional user-defined baseline correction. Can be used to correct for any
offset caused by light scattering particulates by subtracting measured absorbance at
specified baseline correction wavelength from absorbance values at all wavelengths
in sample spectrum. As a result, absorbance of sample spectrum is zero at specified
baseline correction wavelength.