Trace2O AquaSafe WSL25 Plus User manual

AquaSafe
WSL25 Plus
Water Safety
Laboratory
Instruction Manual

Contents
Section 1 : Introduction
Section 2 : Kit Contents
Section 3 : WSL25 Incubator Instructions
Section 4 : Membrane Filtration Instructions
Section 5 : Turbidity Tube Instructions
Section 6 : Comparator Instructions
Section 7 : pH Pocket Tester Instructions
Section 8 : EC Pocket Tester Instructions

Introduction
The Aquasafe WSL25 Plus is a fully portable field laboratory for the detection of microbiological and
physico-chemical water quality parameters.
The lab is housed in a waterproof wheeled carry case with telescopic handle and comprises the
following :
Integrated single chamber digital incubator complete with all accessories and consumables
for carrying out 250 tests for Faecal and Total Coliforms.
Comparator complete with accessories and consumables for 250 tests of Free & Total
Chlorine.
Pocket meter for pH
Pocket meter for EC
Turbidity Tube for turbidity testing in the range 5 to 500NTU.

Kit Contents
2
1
1
9, 10, 11,
12, 13, 14,
15, 16, 17,
18, 19
5
2
4, 5, 6, 7
4
3
Fig 1.0 Main Case components
20
Fig 1.1 Tray components
3
1
1
1
5
5
5
6
9
7
8
10

Main Case Components
Item
Qty
Description
1
1
Turbidity Tube 5-500NTU
2
1
Digital Incubator
3
1
Sampling Cable with Carabiner
4
1
Pen
5
1
Multi-Purpose Screwdriver
6
1
Digital Thermometer
7
1
Vacuum Pump
8
5
Lauryl Sulphate Broth Sachet
9
1
Silicone grease
10
1
Calibration Pack
11
1
Vehicle Charging Cable
12
5
Sample Collection Bag (with dechlorination tablet
13
1
Box Membrane Filters (100 Pack)
14
1
Phenol Red reagents (50 Pack)
15
1
DPD No.1 reagents (50 Pack)
16
1
DPD No.3 reagents (50 Pack)
17
2
Comparator Tube
18
1
HydroLite HL101 pH Pocket Tester
19
1
HydroLite HL102 EC Pocket Tester
20
1
Removable Tray
Tray Components
Item
Qty
Description
1
5
Sterilised water for Membrane Lauryl Sulphate Broth preparation
2
1
5ml Sterile syringe
3
1
30ml Methanol
4
1
Membrane Filtration Unit including, waste beaker, sample beaker, measuring
funnel, sintered glass disk & 3 silicone gaskets
5
3
Container of Pads (100 Pack)
6
1
Forceps
7
1
Vacuum Tube
8
1
Eye Glass
9
1
Pad Dispenser
10
1
Comparator

Section 3

AquaSafe
MSI25
Microbiological
Safety
Incubator
Instruction Manual

1.0 Introduction
The Aquasafe MSI25 is a portable incubator for the incubation of microbiological samples prepared
using the membrane filtration method. The incubator is primarily designed to be used with the
supplied 54mm x 3.5mm aluminium petri dishes which are suitable for 47mm membrane filter pads,
but the incubator can also be used with 55mm pre-prepared plastic petri-dishes.
The incubator is supplied with 20 Petri dishes as standard but has a capacity for up to 25 dishes.
The incubator has the option to run 37°C, 44°C and user defined temperature profiles for periods of
1 –24 hours.

2.0 Incubator set-up
The incubator is integrated into the waterproof carry case with a rechargeable battery and power
supply.
The power pack is comprised of a 12Volt 12Ah Sealed Non-Spillable Lead acid battery. The battery
must be electrically isolated during transport on-board aircraft so to facilitate this the power supply
is disconnected from the battery by removal of the positive(red) terminal connection and the DC
Jack is removed from the incubator socket and fitted with a cap. Prior to use the connector labelled
A must be connected to the positive terminal of the battery labelled B (accessed by removing the
white panel) and the DC Jack ( labelled as such) should be plugged into the socket on the side of the
incubator after removal of the cap.
Please note when transporting via aircraft these connections should be removed to prevent any
risk of short circuit during transit.
It is recommended to charge the battery fully prior to use. To do this remove the power supply unit
from the kit without overstretching the cable and plug into a 240V AC outlet. The indicator on the
charger will illuminate red, orange or green. The states indicate battery condition as follows :
Red : discharged.
Orange : Partially charged.
Green : Fully charged.
To charge the battery from empty takes approximately 4-6 hours.
If desired the incubator can be used whilst the battery is being charged however the battery will
charge at a lower rate.
The incubator is also supplied with a vehicle cigarette lighter cable. To use this the incubator can be
removed from the kit by lifting it up and unplugging the DC jack on the right hand side of the unit.
The cigarette lighter cable is then plugged into this socket. 12V or 24V vehicle systems may be used.

3.0 Operating instructions
3.1 Incubator controls
Petri-Lok cassette
On/Off switch
12V DC Power
socket
Switches
LCD Display
Petri-Lok Cassette.
The Petri-Lok cassette consists of a rack
and a spring loaded top to hold the
petri-dishes in place. To remove the
cassette, twist the cassette to the left
and lift out. The top of the cassette is
removed by pushing down on the top
whilst holding the rack, twisting to the
left and then removing the top. To re-
fit align the arrow with either of the
two slots in the top of the rack, push
down and twist lock into place.
Incubator controls
The incubator has an on/off switch and
power connector on the right hand
side. The on/off switch is pressed in to
turn on and depressed to turn off.
The incubator can be run from a power
supply from 12 –24Volts.
The user interface is via the three
switches and the LCD display on the top
of the incubator.

3.2 Getting started
It is assumed that the user is familiar with the preparation of samples using the membrane filtration
method and as such this manual does not cover this however, instructions on membrane filtration
are available on request.
The user should familiarise themselves fully with the set-up, control and calibration of the
incubator prior to carrying out tests for the first time.
Turn on the power to the incubator using the switch located on the right hand side just above the
12V DC power connector. This is accessed by the recess in the foam to the right of the incubator.
The incubator will beep and the screen will turn on.
The incubator may be in one of three operating modes on power up :
1 : New run
2: Partial incubation cycle (idle)
3: Partial incubation cycle (Run)
If the incubator is in mode 1 or 2 the screen shown in Fig1.0 will be displayed. If the incubator is in
mode 3 the incubator will return to the last know state and auto start the incubation cycle from
where it was before the power was removed. Mode 3 is primarily intended for unattended power
failure conditions.
If Fig 1.0 is displayed and the incubator was in mode 2 then the previous incubation cycle can be
restarted from where it was left by pressing restart. This will be discussed later.
The screen will be displayed for 60seconds before defaulting to the restart condition.
Pressing the right hand switch will reset the incubator ready to set-up and start a new incubation
cycle and the screen will change to the one shown in Fig 1.1.
Pressing the left hand button will step through the screens as illustrated in the cyclic sequence
shown in Figures 1.1 to 1.8.
Each screen and options will now be discussed in further detail.

3.3 Menu sequence
20.9°C 24:00
OFF MEM 10.5v
Select Option
Restart NewRun
20.9°C 24:00
->37°C MEM 10.5v
20.9°C 24:00
->44°C MEM 10.5v
20.9°C 24:00
U->##°C MEM 10.5v
20.9°C 24:00
New <SEL 10.5v
20.9°C 24:00
Cal <ADJ 10.5v
20.9°C 24:00
U## <ADJ 10.5v
20.9°C 24:00
Tim <ADJ 10.5v
Fig 1.0
Fig 1.1
Fig 1.2
Fig 1.3
Fig 1.4
Fig 1.5
Fig 1.6
Fig 1.7
Fig 1.8

3.3.1 Start screen (Figure 1.1)
This screen shows the current status of the incubator.
In the state shown the incubator is in idle mode and not running.
3.3.2 37°C Program (Fig 1.2)
This mode is the pre-programmed default 37°C incubation cycle. In this mode the incubator will run
an 18 hour incubation cycle at 37°C. The 18 hour timer can be altered to any setting between 0 and
24 hours. Adjusting the incubation time is described in section 3.3.6
Pressing and holding the middle switch for 5 seconds will start the incubation cycle and the screen
will change to the following :
The asterisk preceding the temperature will switch on and off indicating the status of the heater.
To stop or pause the incubation cycle press and hold the middle switch again for 5 seconds. The
display will return to the one shown in Fig1.2.
20.9°C 24:00
OFF MEM 10.5v
Current temp
Time remaining
Incubator status
Battery voltage
Mode
20.9°C 24:00
->37°C MEM 10.5v
*20.9°C 24:00
->37°C RUN 10.5v

3.3.3 44°C Program (Fig1.3)
This mode is the pre-programmed default 44°C incubation cycle. In this mode the incubator will run
an 18 hour incubation cycle at 44°C. The 18 hour timer can be altered to any setting between 0 and
24 hours. Adjusting the incubation time is described in section 3.3.6
Pressing and holding the middle switch for 5 seconds will start the incubation cycle and the screen
will change to the following :
The asterisk preceding the temperature will switch on and off indicating the status of the heater.
To stop or pause the incubation cycle press and hold the middle switch again for 5 seconds. The
display will return to the one shown in Fig1.2.
3.3.4 User defined Program (Fig 1.4)
This mode will run an incubation cycle at the user defined temperature for 18 hours. Adjusting the
user defined temperature is described in section 3.3.7. The 18 hour timer can be altered to any
setting between 0 and 24 hours. Adjusting the incubation time is described in section 3.3.6
Pressing and holding the middle switch for 5 seconds will start the incubation cycle and the screen
will change to the following :
The asterisk preceding the temperature will switch on and off indicating the status of the heater.
To stop or pause the incubation cycle press and hold the middle switch again for 5 seconds. The
display will return to the one shown in Fig1.2.
20.9°C 24:00
->44°C MEM 10.5v
20.9°C 24:00
U->##°C MEM 10.5v
*20.9°C 24:00
->44°C RUN 10.5v
*20.9°C 24:00
U->##° RUN 10.5v

3.3.5 Current Run status (Fig 1.5)
In this mode pressing the middle switch will display the following screen which will flash
intermittently :
The time remaining is displayed in the top left corner. This can be reset to the programmed default
cycle time by pressing the left switch. After the switch press it will jump to the screen shown in
Fig1.6. Pressing save will save the current time and jump to the screen shown in Fig1.6. Exiting will
return from this mode.
3.3.6 Incubation cycle time setting (Fig 1.6)
In this mode the default cycle time can be changed from 18 hours to anywhere between 0 and 24
hours.
To adjust the time press the middle switch. The screen will change to :
Pressing the left switch will decrease the time and pressing the right switch will increase the time.
When the desired time has been set press and hold the middle switch to save the settings.
20.9°C 24:00
New <SEL 10.5v
15:45 TIMER A
RST SAVE EXIT
20.9°C 24:00
Tim <ADJ 10.5v
18:00 TIMER A
- Save +

3.3.7 User defined temperature setting (Fig1.7)
In this mode the user defined incubation temperature can be adjusted to a temperature between
20°C and 50°C.
To adjust the temperature press the middle switch. The screen will change to :
Use the left switch to decrease the temperature and the right switch to increase the temperature.
When the desired temperature is set press and hold the middle switch to save the settings.
20.9°C 24:00
U## <ADJ 10.5v
##.#°C User Temp
- Save +

3.3.8 Calibration (Fig 1.8)
In this mode the incubator is calibrated.
To calibrate the unit the following equipment is required:
-Digital thermometer with 100mm x 4mm stainless steel probe (Not included)
-Trace2o Incubator calibration pack (Included)
-Petri-Lok petri-dish cassette.
-10 Aluminium Petri dishes.
Place 10 empty Petri dishes into the bottom of the cassette.
Then place the calibration pack into the cassette.
Fit the spring loaded top and insert the thermometer through
the hole into the top until it is fully inserted. See Fig 3.1.
Fit the cassette into the incubator chamber.
Set the incubator to the desired mode of operation –37/44/User, and start
the incubation cycle. Wait at least 1 hour.
Compare the displayed temperature on the incubator screen to that shown on the thermometer. If
there is a difference > 0.2°C the calibration should be adjusted as follows :
Exit the run mode and step through the screens until the calibration screen is displayed. Press the
middle switch to enter calibration mode. The following screen will be displayed.
Adjust the temperature up or down using the left and right switches until it matches the
temperature displayed on the thermometer. Press the middle switch to save the settings.
Step through the screens until the mode previously been used is displayed and start the incubation
cycle again.
Allow the incubator time to adjust the temperature which could take up to 30mins and check the
calibration again. If the original temperature was significantly different to the thermometer then the
calibration steps may need to be repeated two or three times.
20.9°C 24:00
Cal <ADJ 10.5v
##.#°C Calibrate
- Save +
Fig 3.1

4.0 Care and Maintenance
The Aquasafe incubator is designed to require minimal maintenance although from time to time
cleaning will be required.
To clean the incubator outer surfaces and plastics use a cloth moistened with a mild soap solution or
an alcohol impregnated cloth or wipe. Do not use acetone or other solvents.
The incubator is splash proof but it is not fully waterproof so it should not be left outside in wet
conditions and must not be submersed in water.
The incubator chamber is sealed at the bottom so spills will not affect the unit but should be cleaned
as soon as possible. The Aluminium chamber can be cleaned with Alcohol, soap or solvent based
cleaners.
The Petri-Lok rack can be steam sterilised if required but it is not recommended to steam sterilise
the top.
5.0 Guarantee and Assistance
Trace2o hope that the Aquasafe incubator will give many years of trouble free operation but in the
event of a technical problem occurring the Aquasafe incubator is covered by Trace2o Ltds standard
Guarantee terms and conditions available via email or via download from www.trace2o.com.
In the event that any technical assistance is required Trace2o Customer service department will be
happy to assist. Contact details as follows :
Trace2o Ltd
The Technology Centre
Wagtech Court
Station Road
Thatcham
Berkshire. RG19 4HZ
UK.
T: 01635 866772
E: [e-mail]