Turner Cyclops 6K User manual

User’s Manual
May 18, 2018
P/N 998-2100
Revision 2.9
TURNER DESIGNS
1995 N. 1st Street
San Jose, CA 95112
Phone: [phone]
FAX: [phone]

Table of Contents
1.
Introduction
1.1
Description
4
2.
Inspection and Setup
2.1
Instrument Checklist
5
2.2
Housing Configurations
5
2.3
Optional Accessories
6
2.4
Functional Test for Cyclops with connector
7
3.
Measurements with the Cyclops
3.1
Introduction
8
3.2
Setting the Gain
8
3.2.1
Gain Determination Procedure
8
3.2.2
Static Gain Control
9
3.2.3
Auto Gaining
9
3.3
Direct Concentration Calibration
9
3.3.1 Direct Concentration Calibration for Chlorophyll Cyclops
3.3.2 Direct Concentration Calibration for Turbidity Cyclops
10
10
4.
Maintenance and Warranty
4.1
Maintenance
11
4.1.1
Rinsing
11
4.1.2
Care for the bulkhead connector
11
4.1.3
Cleaning the Optics
11
4.2
Warranty Terms
11
4.3
Warranty Service
12
4.4
Out of Warranty Service
12
Appendices
A
Specifications
14
B
Recommended Measurement Practices
15
C
Wiring Guide
16
D
Pigtail Cable and Connector Information
17
E
Controlling the Gain
18
F
Linear Range, Quenching and Temperature Considerations
19
G
Functional Test for Cyclops with no Connector
20
H
Using the Cyclops Submersible Sensor with the DataBank
21

WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) DIRECTIVE
Turner Designs is in the business of designing and selling products that benefit the
well-being of our environment. Accordingly, we are concerned with preserving the
surroundings wherever our instruments are used and happy to work with customers by
complying with the WEEE Directive to reduce the environmental impact resulting from
the use of our products.
WEEE Return Process:
To arrange the return of an end-of-life product, proceed as follows:
If you purchased your instrument through a Turner Designs Distributor please
contact your local representative. They will instruct you where to return the end-of-
life product.
If you purchased your instrument directly from Turner Designs please contact
Turner Designs Customer Service
By Phone: [phone] or Toll Free: [phone]
By Email: Customer Service at [e-mail]
Turner Designs will provide a WEEE RMA Number, a Shipping Account Number,
and a Ship to Address. Package and ship the product back to Turner Designs.
The product will be dealt with per Turner Designs’ end-of-life recycling program in an
environmentally friendly way.

Cyclops Submersible Sensor
998-2100 Rev. 2.9 Page 4
1. Introduction
1.1 Description
The Turner Designs Cyclops Submersible Sensor is an accurate single-channel
detector that can be used for many different applications. It is designed for
integration into multi-parameter systems from which it receives power and
delivers a voltage output proportional to the concentration of the fluorophore,
particle, or compound of interest.
The Cyclops voltage output can be correlated to concentration values by
calibrating with a standard of known concentration.
Effective April 1, 2017 all Cyclops-7 shipping will be Cyclops-7F. Updated
to have a faster settling time, Cyclops-7F is ready to make measurements
1 second after power up.

Cyclops Submersible Sensor
998-2100 Rev. 2.9 Page 5
2. Inspection and Setup
2.1 Instrument Checklist
The Cyclops Submersible Sensor shipment package consists of:
Cyclops Submersible Sensor:
Configured and factory scaled for the specified analysis (see Identification
Letter stamped on the connector for specified analysis):
“C” = Chlorophyll
“R” = Rhodamine
“F” = Fluorescein
“P” = Phycocyanin
“E” = Phycoerythrin
“U” = CDOM / FDOM
“O” = Crude Oil
“B” = Optical Brighteners
“T” = Turbidity
“G” = Refined Fuels
“A” = PTSA
“L” = Tryptophan
“D” = Red Excitation
Test Criteria
2.2 Housing Configurations:
Stainless Steel Cyclops-7F (P/N: 2110-000-“Identification Letter”)
Stainless Steel Cyclops-7 (P/N: 2100-000-“Identification Letter”)
Plastic or Titanium housings (recommended for highly corrosive
environments or long term deployments).
Titanium Cyclops-7F (P/N: 2110-000-“Identification Letter” T)
Plastic Cyclops-7F (P/N: 2118-000-“Identification Letter”)
Plastic Cyclops-7F with Titanium connector (P/N: 2118-000-“Identification
Letter”T)
Titanium Cyclops-7 (P/N: 2100-000-“Identification Letter” T)
Plastic Cyclops-7 (P/N: 2108-000-“Identification Letter”)
Plastic Cyclops-7 with Titanium connector (P/N: 2108-000-“Identification
Letter”T)
6000 meter Cyclops-6K (P/N: 2160-000-“Identification Letter”)
No Connector Cyclops-7F (P/N: 2110-000-“Identification Letter”-NC).
No Connector Cyclops-7 (P/N: 2100-000-“Identification Letter”-NC).
Refer to Appendix G for wiring and functional testing.
2.3 Optional Accessories include:
Cyclops Pigtail Cables with Locking Sleeve (see Appendix D for more
information)
“A”= PTSA“A”= PTSA

Cyclops Submersible Sensor
998-2100 Rev. 2.9 Page 6
0.6 meter Pigtail Cable with Locking Sleeve (P/N 2100-750)
5 meter Pigtail Cable with Locking Sleeve (P/N 2100-755)
10 meter Pigtail Cable with Locking Sleeve (P/N 2100-751)
25 meter Pigtail Cable with Locking Sleeve (P/N 2100-752)
50 meter Pigtail Cable with Locking Sleeve (P/N 2100-753)
Note: Turner Designs recommends you do NOT tow sensors with
cables.
DataBank Handheld Data Logger (see Appendix H) (P/N 2900-000)
Flow Cap (see Accessory Instructions on USB Flash Drive)
Cyclops Stainless Steel and Titanium (P/N 2100-600)
Cyclops Plastic (P/N 2100-608)
Cyclops-6K (P/N 2160-600)
Shade Cap (see Accessory Instructions on USB Flash Drive)
Note: We recommend use of the shade cap as it provides a fixed
distance for sample measurement and minimizes effects from ambient
light.
Cyclops Stainless Steel and Titanium (P/N 2100-701)
Cyclops Plastic (P/N 2100-708)
Cyclops-6K (P/N 2160-700)
Cyclops-7 Chlorophyll Standard Solution, Blue Excitation (p.n. 2100-320)
Note: Not for use with Cyclops-6K.
Solid Secondary Standard (SSS) for in vivo Chlorophyll, Phycocyanin,
Phycoerythrin, Rhodamine, Fluorescein (see Accessory Instructions on USB
Flash Drive)
Cyclops Stainless Steel and Titanium (P/N 2100-900)
Cyclops Plastic (P/N 2100-908)
Cyclops-6K (P/N 2160-900)
Solid Secondary Standard (SSS) for UV Sensors (CDOM / FDOM, Optical
Brighteners, Refined Fuels and Crude Oil) (see Accessory Instructions on
USB Flash Drive)
Cyclops Stainless Steel and Titanium (P/N 2100-904)
Cyclops Plastic (P/N 2100-905)
Cyclops-6K (P/N 2160-901)
Flow Cap
Solid Secondary Standard
Shade Cap

Cyclops Submersible Sensor
998-2100 Rev. 2.9 Page 7
2.4 Functional Test for Cyclops with connector
To perform a functional check on the Cyclops, connect the Pigtail colored wires
to the power supply and multi-meter as shown in Figure 1 below.
Additional Equipment required for functional tests:
DC Power Supply, 3 - 15 VDC, >100 mA
Multi-meter to read 0 –5 VDC
Note: Supply voltages greater than 15 VDC will result in damage to
the sensor.
Figure 1.
With the Cyclops connected as shown in Figure 1 answer questions 1-3 by
making the following functional tests:
1. Is the LED on?
Hold a piece of white paper about ½ an inch in-front of the optical head to
ensure the LED is on. Note: Cannot perform this test for Turbidity
sensors because they use IR which is not visible.
2. Is there voltage output?
The multi-meter should be reading some voltage >0 VDC
3. Does the voltage output change?
Move the light source closer to your hand or a surface and check if the
voltage output increases
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops Pigtail cable holes
3
6
4
2
1
55
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops Pigtail cable holes
33
66
44
22
1
55 5
2
4
4
6
1
333
66
44
22
1
55 5
2
4
4
6
1
3
Cyclops pins / wires
3
4
2
1
5
6
6
3
4
2
1
5
6
6
33
44
22
1
55
6
6
Cyclops pins / wires
3
4
2
1
5
6
6
33
44
22
1
55
6
6
33
44
22
1
55
6
6
33
44
22
1
55
6
6
12.00 .015
+ -
DC Power Supply
PSU Positive Connection
(Red) Supply Ground
0 VDC (Black)
3.52
Multimeter
10 VDC
+-
Signal Output
“+”(White)
Analog Ground
“-”(Green)
Blue
(Tie to Green for 10X) Brown
(Tie to Green for 100X)
X1(Low) Gain
Leave both wires disconnected
for Functional Check
Cyclops Pigtail Cable
12.00 .015
+ -
DC Power Supply
12.00 .015
+ -
DC Power Supply
PSU Positive Connection
(Red) Supply Ground
0 VDC (Black)
3.52
Multimeter
10 VDC
+-
3.52
Multimeter
10 VDC
+-
Signal Output
“+”(White)
Analog Ground
“-”(Green)
Blue
(Tie to Green for 10X) Brown
(Tie to Green for 100X)
X1(Low) Gain
Leave both wires disconnected
for Functional Check
Cyclops Pigtail Cable

Cyclops Submersible Sensor
998-2100 Rev. 2.9 Page 8
3. Measurements with the Cyclops
3.1 Introduction
The following information will describe how to:
oDetermine and set the appropriate gain
oCalibrate the Cyclops using standards with known concentrations
oMake measurements with the Cyclops
oUse the Solid Secondary Standard
Note: To make accurate and repeatable measurements it is important to
keep the sensor clean; see section 4.1 for information on cleaning your
sensor.
3.2 Setting the Gain
Gain setting refers to the sensitivity configuration of the sensor. There are three
gain settings; X1, X10 and X100. As the gain increases, the sensitivity
increases and the concentration range decreases.
3.2.1 Gain Determination Procedure
1) For in vivo applications, take a natural sample of water from a
sampling station where you plan to deploy the Cyclops. Applying
good measurement practices, store it properly and quickly transport it
to a laboratory where you have the Cyclops connected to a multi
meter and DC power source (see Figure 1).
2) Pour the water sample into a clean glass beaker and submerge the
optical end of the Cyclops (see Appendix B for “Recommended
Measurement Practices for using your Cyclops in the Lab” for how
best to accomplish these steps).
3) Activate the X10 gain setting (see Wiring Guide Appendix C) if you
believe the sample to represent a typical condition. You would like to
obtain a signal from the sample that is significantly higher than a
blank sample (de-ionized water or filtered seawater), but not a signal
that is close to the maximum of 5 Volts.
4) If the sample signal is high, (>3.0 V for example) you may choose to
use the X1 gain instead of the X10 gain setting so that you avoid
going over scale once you deploy the Cyclops.
5) If the sample signal is very low (<0.3V) you may choose to use the
X100 gain setting to achieve higher sensitivity but a smaller
measurable range
This process is easier for dye tracing applications. Simply create the dye
dilution of interest and record what signal level it provides on the three
gain settings.

Cyclops Submersible Sensor
998-2100 Rev. 2.9 Page 9
3.2.2 Static Gain Control
If integrating into a multi parameter system or data logger that is set up
for “Static Gain Control”, which refers to the use of only one gain setting
at a time, then you must determine which gain to use prior to deployment
(see section 3.2.1) and have an integration cable made to activate that
specific gain (see Appendix C). For most applications the X10 gain will
provide the best sensitivity, range, and resolution.
Customers wanting to dynamically change the gain ranges to achieve
the optimum operating range should refer to “Method 2 – Dynamic Gain
Control” in Appendix E on how to interface with a Data Collection System
with programmable outputs.
3.2.3 Auto Gaining
Certain data loggers or multi parameter systems will have an auto
gaining feature which will automatically adjust the sensitivity according to
the voltage output from the Cyclops sensor. This feature maximizes the
performance of Cyclops sensors allowing users to detect a broad range
of concentrations, obtain the best resolution, and read minimum
detection limits without having to rewire or manually change the sensor’s
sensitivity. Turner Designs manufactures the DataBank Handheld Data
Logger (see Appendix H) which has the auto gain feature and other
functions that maximize the performance of Cyclops sensors.
3.3 Direct Concentration Calibration
Calibrating the Cyclops is a simple process requiring the use of calibration
standards. The Cyclops can be calibrated using a single calibration standard
which correlates the standard’s known concentration to the voltage measured
for that specific standard:
1) Connect the Cyclops to a power source and set the Cyclops to a gain setting
(see section 3.2.1 for explanation on how to determine gain)
2) Measure the voltage from a blank sample for the configured gain setting.
Note: A good blank to use for this application is ultra pure or
deionized water.
3) Use a standard of known concentration and create a correlation between the
standard’s concentration and the sensor’s voltage response.

Cyclops Submersible Sensor
998-2100 Rev. 2.9 Page 10
4) Once a correlation has been made, use the following equation to calculate
concentration estimates for the calibrated gain:
CSample = [(CStd)/(VoltsStd - VoltsBlank)] * (VoltsSample –VoltsBlank)
CStd = Concentration value of standard used for calibration
Csample = Concentration of sample
VoltsStd = Voltage reading from standard concentration
VoltsSample= Voltage reading from sample(s)
VoltsBlank = Voltage reading from blank
3.3.1 Direct Concentration Calibration for Chlorophyll Cyclops
Turner Designs sells Cyclops Chlorophyll Standard Solution, Blue
Excitation P/N 2100-320 for direct concentration calibration. This
standard represents an equivalent chlorophyll concentration of 40 µg/L.
Follow instructions in section 3.3 to establish a correlation between the
standard and the sensor’s voltage response. Use the equation from step
4 of section 3.3 to calculate chlorophyll estimates as µg/L in the
calibrated gain.
Note: This calibration is an approximation. Actual µg/L estimates
will vary depending on factors such as speciation, light history,
algal stresses, pigment ratios, etc.
3.3.2 Direct Concentration Calibration for Turbidity Cyclops
Turner Designs recommends Amco Clear Turbidity Standards. They are
non-toxic safe solutions consisting mainly of deionized water that comes
prepared in a broad range of concentrations and have a shelf life
guaranteed for one year. These standards, optimized for the Cyclops,
are available from GFS Chemicals under part numbers 8506 (10 NTU),
8507 (100 NTU), and 8620 (1000 NTU). Follow instructions below to
establish a correlation between the standard’s concentration and the
sensor’s voltage response. Use the equation from step 4 of Section 3.3
to calculate turbidity values in NTU for the calibrated gain.