U.S. Solid USS-FX User manual

ULTRASONIC PROCESSOR
MANUAL
MODEL: USS-FX
is manual should be made available to all users of this equipment. For best results, and for maximum durability of the
equipment, carefully read and follow all instructions. Failure to do so can lead to serious injury or catastrophic damage to
the user, machine, supplies, or surrounding areas. All safety suggestions must be followed closely, and extreme precaution
must be taken to assure proper use of the equipment by only qualied personnel who have read this guide.
For questions or concerns, e-mail [email protected], visit www.ussolid.com,
or call [phone]

Contents
•Summary…………………………….................…………………………………I
The Principle of Working….....……..……......................................................II
Product Features……………………...……..................................................III
Common Applications…….................………………………….……………IV
Technical Parameters………….............................………………...………...V
Appearance Display……………................................………....…………...VI
Energy Converter Transducer……………..................………………..…….VII
•Installation……………………....................................…………………..……VIII
Warnings and Notes…………………………….....……………………...…….X
Removing Amplitude………………....………………………..…………….....XI
Installing Amplitude...................………….......…………………......……......XII
Packing List…………............…………………………………………………...XIII
Operation……………………....................................………………….....……IX

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I. Summary
II. The Principle of Working
Ultrasonic homogenizers are multi-function and multi-purpose
instruments that utilize intense high-frequency sound to induce
cavitation in liquid. As the cavitation bubbles collapse, high shearing
energies disrupt animal and plant tissue, lyse yeast, bacteria and
spores. The ultrasonicator can also be used for non-biological
applications such as emulsification, nanoparticle dispersion, intense
washing or acceleration of chemical reactions (sonochemistry).
This machine is made up of an ultrasonic power supply and an
ultrasonic transducer assembly (generator or probe). The ultrasonic
power supply converts the input single-phase electricity of 110 VAC, 60
Hz into an alternating electrical signal of 20-25 kHz. The circuitry
consists of a power rectifier, power switcher, a frequency conversion
system, power amplifier, phase-locked frequency automatic tracking
device, power regulator, power detector, power protector device, and
microcomputer controller.
Driven by the appropriate impedance and power, the transducer
subassembly generates mechanical energy through a piezoelectric
resonator which is focused and amplified with a tuned, titanium metal
horn (probe). When the probe tip is immersed in the sample solution,
intense, high-frequency sound waves coming from the tip of the probe
induce cavitation. As the cavitation “bubbles” collapse, high shear
forces break or tear open the cells. Indeed, cavitation forces are high
enough to break covalent bonds. Hence, ultrasonicators can be used to
fragment high molecular weight DNA.

III. Product Features
1. Automatic frequency tracking routinely scans and tracks within 20KHz
frequency.
2. Auto-tuning for convenience of use and optimal processing efficiency
3. Temperature indicator and controller
4. 99h total working time controller, 1s – 99h, count-down time display
5. Automatic amplitude compensation
6. Power-emitted display for accuracy and repeatability, variable power
output
7. Integrated soundproof box to reduce cavitation sound emitted during
processing
8. 7-inch color industrial touch screen applied and all functions integrated
display
IV. Common Applications
9. Mixing, blending, emulsifying, and homogenizing solutions
10. Reducing the particle size of suspensions
11. Dispersing suspensions
12. Disrupting cells
13. Catalyzing chemical reactions

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V. Technical Parameters

V. Technical Parameters

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VI. Appearance Display
2. Touch Screen 3. Handle
1. Host
4. Ultrasonic Probe 5. Sample Observation Window
6. Power Button
8. Temperature Probe Connecting Button
7. Power Cable Connector

VII. Energy Converter Transducer
3. Piezoelectric Ceramics
1. High Tensile Screw
4. Amplitude
2. Electrode Plate
5. Connecting Screw
VIII. Installation
1. Have the bracket and clamp assembled (the holding system for the
transducer).
2. Have the transducer cable and transducer connected, then connect them
with the main machine (ultrasound generator).
3. Connect the power cable to the power supply and main machine.
4. Put the transducer unit on the bracket or soundproof box. Make sure the
ultrasonic probe tip is inserted into the sample liquid surface and in the
center of the container. The probe should get into the liquid 5 mm to 10
mm beneath the liquid surface. For large sample volumes, the distance
between the probe tip and the container’s bottom should be bigger than
30 mm. (The ultrasonic probe tip can be as close as 10 mm from the
bottom of the container when processing a small sample size or when
using a lower transducer power setting) If you hold the transducer unit to
process the sample, hold it on SLEEVE position. Other positions are not
allowed.
It is prohibited to start ultrasonic output when the probe tip is
out of the liquid. Otherwise the energy converter and
ultrasonic wave generator will be damaged.

IX.Operation
Up Button: You can increase power by pressing the Up button.
Down Button:
You can decrease power by pressing the Down button.
Press the ON/OFF button to start or pause the
working process.
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IX. Operation
By pressing the Settings button, you will move to below interface.
1. Press User to set user code in the machine.

IX. Operation
Total Working Time: Total time that machine works.
Pulse-On Time: Time that machine produces ultrasound.
Pulse-Off Time: Time that machine is on but stops producing ultrasound.
Power: The percentage of the power available.
Shutdown Temp: The machine will stop working when it reaches the set
temperature.
Save: Save the settings.
Back: Go back to the previous interface.
There are three modes:
1. Continuity Mode: It will not stop working if you do not stop the machine.
2. Time Mode: The working process will stop when it reaches the set time.
3. Pulse Mode: Machine works when the pulse is on and pauses when the
pulse is off.
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X. Warnings and Notes
1. NEVER START ULTRASONIC OUTPUT WHEN THE PROBE TIP IS EXPOSED IN
THE AIR
2. The apparatus swithes the power supply without industrial frequency
transformer. Don’t randomly touch after opening the generator housing to
avoid electric shock.
3. The apparatus does not need to warm up and should be grounded well
when being used.
4. Use it in an environment free from moisture, sunshine and corrosive gases.
5. Be sure the platform is tightly fixed to the rod, and the ultrasonic probe is
in liquid and not touching the walls of the sample vessel.
6. The liquid temperature will increase rapidly due to the cavitation effect
during crushing. The user should pay attention to the temperatures for
different cells. It is suggested that the user adopt multiple short time
crushing (not longer than 5s each time) and ice bath cooling.
7. It is practice-proven that multiple short-ime operations, working time 1-2s
and interval time 1-2s, have better effects than continuous long-time
operations. The long interval time can be set to avoid the heating of liquid.
Additionally, continuous long-time operations are subject to no load
operations to shorten the apparatus service lifetime.
8. The horn end will become rough due to cavitation corrosion after being
used for a period of time. Use oil stone or rasper to smooth it. Otherwise,
it will influence the working effects.
9. In general, the volume of homogenization media should be 3 to 10 times
the net volume of the solid sample. Pre-chopping the sample into pieces
less than 1 mm in cross-section will significantly decrease the time of
homogenization.

10. Heating of the sample occurs with long runs. Gross sample heating can be
minimized by:
A. Nesting the sample vessel in an outer vessel containing ice.
B. Select longer interval (gap) times.
C. Using a specially fabricated leptosomatic ultra-sonication vessel
(available as an accessory).
The user should keep in mind that transient (millisecond) heating and free
radical formation in the immediate vicinity of the ultrasonic probe tip is
unavoidable. Please take this into consideration when working with samples
especially sensitive to heat or free radical damage. To avoid excessive
cavitation leading to overload when using smaller ultrasonic probes of 2,
3, and 6 mm tip diameter, the ultrasonic power wheel should be rotated to a
lower power setting.
11. If the liquid volume containing the sample is less than 5 ml, choose an
ultrasonic probe with a tip diameter of 2 to 3 mm. Smaller probes are
inserted into liquid at a depth about 1 cm,and the distance between the tip of
the ultrasonic probe and container bottom will vary to some extent by the
power setting selected for the probe. However, it should not be less than 0.5
cm.
X. Warnings and Notes continued
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XI. Removing Amplitude
2. Buckle 2
1. Buckle 1
5. Amplitude
3. Wrench 1
6. Workbench
4. Wrench 2
7. Cushion
Place energy converter on the bench covered by soft materials (e.g. towels). Put
the small wrench into the wrench hole on the horn and the big wrench into the
wrench hole on the energy converter. The big wrench should be placed towards
the left and the small wrench towards the right.
Facing the horn with the big wrench in your left hand and the small wrench in
your right hand, loosen by turning downwards with two hands at the same
time. When changing the horn, if the M10 screw is attached to the horn,
manually unscrew the screw from the lever and screw the screw 1/2 way into
the energy converter.
Then screw on the required horn. If the screw cannot be manually screwed out
from the horn, knock the screw lightly on wooden materials so that the screw
can be screwed out manually.

3. Wrench 11. Buckle 1 2. Buckle 2
6. Workbench
4. Wrench 2
7. Cushion
XII. Installing Amplitude
5. Amplitude
1. Screw amplitude manually.
2. Place energy converter on the bench covered by soft materials (e.g.
towels).
3. Put wrench1 on buckle2 and wrench2 on buckle2.
4. Facing the horn with wrench1 in the left hand and wrench2 in the
right hand, tighten by turning downwards with two hands at the
same time.
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XIII. Packing List