UGO BASILE PAM User manual

P.A.M.
PRESSURE APPLICATION MEASUREMENT
Cat. No. 3 8 5 0 0

UGO BASILE
BIOLOGICAL RESEARCH APPARATUS INSTRUCTION MANUAL
Via G. Borghi 43
21025 COMERIO - Varese, ITALY P.A.M.
Pressure Application Measurement
Cat. No. 3 8 5 0 0
Series No. Mfg. date
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ALTHOUGH THIS INSTRUMENT HAS BEEN DESIGNED WITH INTERNATIONAL SAFETY
STANDARD, THIS MANUAL CONTAINS INFORMATION, CAUTIONS AND WARNINGS WHICH
MUST BE FOLLOWED TO ENSURE SAFE OPERATION AND TO RETAIN THE INSTRUMENT
IN SAFE CONDITIONS.
SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSONNEL,
AUTHORIZED BY UGO BASILE ORGANIZATION.
ANY ADJUSTMENT, MAINTENANCE AND REPAIR OF THE OPENED INSTRUMENT UNDER
VOLTAGE SHOULD BE AVOIDED AS MUCH AS POSSIBLE AND, WHEN INEVITABLE,
SHOULD BE CARRIED OUT BY A SKILLED PERSON WHO IS AWARE OF THE HAZARD
INVOLVED.
CAPACITORS INSIDE THE INSTRUMENT MAY STILL BE CHARGED EVEN IF THE
INSTRUMENT HAS BEEN DISCONNECTED FROM ITS SOURCE OF SUPPLY.
Instruction Manual dated April 2010
Revision 1

PAM
PRESSURE APPLICATION MEASUREMENT
Cat. No. 3 8 5 0 0
Main Features:
User-controlled application of pressure directly to the joint
Automatic recording of Limb Withdrawal
Resolution: 0.1 g
Maximum Applicable Force: 1500 gf
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- J JO OI IN NT T P PA AI IN N
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- A AR RT TH HR RI IT TI IS S
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Me ec ch ha an ni ic ca al l P Pa ai in n T Th hr re es sh ho ol ld d i in n: :
J
Jo oi in nt t H Hy yp pe er rs se en ns si it ti iv vi it ty y
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Ch hr ro on ni ic c J Jo oi in nt t I In nf fl la am mm ma at ti io on n
GENERAL
The new P.A.M. (Pressure Application Measurement)
device from Ugo Basile is a novel, easy-to-use tool for
measuring mechanical pain threshold in experimental
joint hypersensitivity models in rodents.
The PAM device has been designed and validated
specifically for the mechanical stimulation and
assessment of joint pain, and therefore is especially
useful in studying arthritis. The PAM device applies a
quantifiable force for direct stimulation of the joint
and for automatic readout of the response.
The operator simply wears a special force sensor on
his or her thumb and measures the force which elicits
the animal response (normally, limb withdrawal).
Each PAM device comes standard with two force
sensors, which have been specially designed to apply
force to rat and mouse joints. The area stimulated
using the small sensor is useful for mice. The large
sensor is useful for stimulating either mice or rat
joints.

UGO BASILE S.R.L.
BIOLOGICAL RESEARCH
APPARATUS
CHECK-LIST
PAM PRESSURE APPLICATION MEASUREMENT
CLIENTE / CUSTOMER______________________________________________
Ordine No. / Order No. ____________________ Data / Date____/_____/______
Cat. 38500 P.A.M. Pressure Application Measurement
UB code CAT.No.
 Q.ty DESCRIPTION DESCRIZIONE
38500-306 1 INSTRUCTION MANUAL MANUALE D’ISTRUZIONE
38500-001 1 ELECTRONIC UNIT UNITA’ ELETTRONICA
38500-002 1 JOINT TRANSDUCER (large) TRASDUTTORE (large)
38500-003 1 JOINT TRANSDUCER (small) TRASDUTTORE (small)
38500 1
SOFTWARE SOFTWARE
38500-323 1 EXTERNAL TRIGGER SWITCH TRIGGER ESTERNO
52010-325 1 USB LEAD CAVO USB
OPTIONAL 38500-006 0 PAW TRANSDUCER TRASDUTTORE DA ZAMPA
DATE / / Serial No. IMBALLATO DA / PACKED BY
Universal Input 85-264 VAC, 50-60Hz
IMPORTANT/IMPORTANTE:
Check the shipment for completeness immediately after receipt: should you find any discrepancy, please fill in the following part and transmit it
to our fax no. [phone]
Al ricevimento della merce controllate che la spedizione sia completa: in caso di discrepanza, completate il formulario di seguito riportato ed
inviatelo al nostro fax no. 0332 745488
FROM: Name Company/Institution
NOTES
DATE REF.
MOD.04 REV 0

UGO BASILE - ITALY Page i
CONTENTS
C O N T E N T S
1GENERAL.....................................................................................................................1
1.1PRINCIPLE OF OPERATION............................................................................................1
2INSTRUMENT DESCRIPTION ................................................................................2
2.1ELECTRONIC UNIT.........................................................................................................3
2.2JOINT TRANSDUCERS (SMALL AND LARGE)...............................................................4
2.3PAW TRANSDUCER ........................................................................................................4
3INSTALLATION..........................................................................................................5
3.1UNPACKING &PRELIMINARY CHECK..........................................................................5
3.2NOTES ON THE INSTRUCTION MANUAL.......................................................................5
3.3CHARGING THE INTERNAL BATTERY...........................................................................6
3.4CONNECTIONS ...............................................................................................................6
3.5INSTALLING THE PAM SOFTWARE...............................................................................6
3.6ADDITIONAL SAFETY CONSIDERATION........................................................................6
4OPERATION ................................................................................................................7
4.1FIRST TIME OPERATION ................................................................................................7
4.2ROUTINE OPERATION OF THE INSTRUMENT ..............................................................10
4.2.1  T URNING THE INSTRUMENT ON 10
4.2.2  A UTOMATIC DIMMING OF THE DISPLAY 10
4.2.3  M ENU OPERATION 10
4.2.4  T URNING THE INSTRUMENT OFF 11
4.3MAIN MENU .................................................................................................................11
4.4MEASURE MENU..........................................................................................................11
4.4.1  M EASURE P EAK F ORCE 12
4.4.2  V IEW A CQUIRED D ATA 14
4.4.3  M EMORY S TATUS 14
4.4.4  T RANSFER D ATA TO PC 14
4.4.5  E RASE M EMORY 14
4.4.6  B ACK TO M AIN M ENU 15
4.5CONFIGURE MENU.......................................................................................................15
5OTHER IMPORTANT FEATURES OF PAM........................................................17
5.1BATTERY VOLTAGE WARNING ...................................................................................17
5.2MEMORY LIMIT WARNING...........................................................................................17
5.3CALENDAR CLOCK.......................................................................................................17
5.4APPLIED FORCE RATEMETER ......................................................................................18
5.5AVAILABLE CONFIGURATION SETTINGS ...................................................................18
5.6UPDATING THE PAM FIRMWARE................................................................................19

UGO BASILE - ITALY Page ii
CONTENTS
5.7SKIPPING THE INTRODUCTION................................................................................... 19
6USING THE PC SOFTWARE PROVIDED WITH PAM..................................... 20
6.1STARTING THE PAM PC SOFTWARE......................................................................... 20
6.2ONITORING PAM TESTS ............................................................................................. 21
6.3DOWNLOADING DATA FROM PAM................................................................................... 23
6.4ANALYZING DOWNLOADED DATA............................................................................... 24
6.5EXCEL FILES................................................................................................................ 26
7MAINTENANCE ....................................................................................................... 26
7.1LONG INACTIVITY ....................................................................................................... 26
7.2CUSTOMER SUPPORT ................................................................................................. 27
8ORDERING INFORMATION.................................................................................. 27
9BIBLIOGRAPHY....................................................................................................... 28
9.1METHOD PAPER .......................................................................................................... 28
9.2OTHER PAPERS MENTIONING UB PAM ................................................................... 28
FIGURE INDEX
Figure 1 "Joint Transducers" ................................................................................................................................................1
Figure 2 "Paw Transducer"...................................................................................................................................................2
Figure 3 PAM “Standard Package”.......................................................................................................................................3
Figure 4 “Electronic Unit and Small Joint Transducer”.............................................................................................................4
Figure 5 “Large and Small Joint Transducers”........................................................................................................................4
Figure 6 “Paw Transducer”...................................................................................................................................................5
 

UGO BASILE - ITALY Page iv
CONTENTS