UniPix ATMOS 1000 User manual

UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&1/9&
ATMOS 1000
Pipette Accuracy Tester instrument
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Operation*Manual**
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Made&in&Switzerland&
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"""#$%&'&()*+,#-./!!!!! ! &%0.1$%&'&()*+,#-./!

UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&2/9&
Disclaimer
• The$ Pipette$ Accuracy$ Tester$ATMOS$is$ designed$ for$ the$ specific$ use$ of$ testing$ and$ calibrating$ air$
displacement$laboratory$pipette$and$no$other$use$is$recommended.$
• The$user$must$read$and$understand$this$manual$before$operation$
• This$instrument$creates$items$that$should$only$be$used$in$a$laboratory$environment.$
• Do$not$open$or$attempt$to$repair$the$instrument$without$expressed$and$explicit$instructions$from$UniPix.$$
• Don't$use$the$instrument$in$an$atmosphere$with$a$danger$of$explosion.$
• Do$not$allow$unauthorized$and$or$untrained$operatives$to$use$this$device.$
• Any$misuse$will$be$the$sole$responsibility$of$the$user/owner$and$UniPix$assumes$no$implied$or$inferred$
liability$for$direct$or$consequential$damages$from$this$instrument$if$it$is$operated$or$used$in$any$way$other$
than$for$which$it$is$designed.$
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Starting ATMOS
The$UniPix$Atmos$Pipette$Accuracy$Tester$is$an$instrument$for$precise$measurement$air$displacement$pipette,$
without$ need$ of$ liquid.$ It$ is$ based$ on$ comparison$with$ a$ calibrated/internal/ reference/ cavity$in$ the$
instrument.$It$also$measures$the$leak$rate$of$pipettes.$$
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Never%use%the%instrument%with%liquid%in%the%pipette%tip.%%
This%will%permanently%damage%the%instrument%(no%repair%is%possible%!)$
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Let%the%internal%temperature%to%stabilize%during%at%least%30%minutes%
after%unpacking%or%moving%the%instrument%from%another%room.$
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• Press$the$main/switch$on$the$front$of$the$instrument$to$power$on.$Press$again$to$switch$OFF.$
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• It$is$recommended$to$verify$that$the$instrument$battery$is$well$charged$or$use$the$instrument$with$the$
external$power$supply$connected$via$the$USB$cable.$
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• The$instrument$is$powered$by$an$internal$Li-ion$battery$which$allows$continuously$operating$during$
more$than$3/hours$and$several$weeks$with$short$daily$testing$sessions.$
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• After$powering$on,$the$instrument$runs$a$self-test$procedure$for$about$10$seconds.$$
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• When$not$operated,$the$instrument$automatically$turns$OFF$after$5$minutes.$
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• ATMOS$ touch$ screen$ is$ better$ responsive$ when$ the$ finger$ is$ applied$ with$
larger$contact$area$
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UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&3/9&
Pipette size selection panel
Select$the$pipette$size$you$are$testing:$
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Note:&The&accuracy&of&P5&testing&is&not&guaranteed&with&ATMOS&1000&instrument.&Another&instrument&
(ATMOS&Nano)&is&designed&for&pipettes&below&P10.&
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Main panel
After$pipette$selection,$or$after$measurements,$you$will$see$the$main$panel.$
From$this$panel,$you$can$reach$the$following$functions:$
• The$Insert/Tip$button$will$start$the$measurement$procedure.$Please$insert$the$tip$and$be$ready$to$
actuate$the$piston$BEFORE$touching$the$button.$
• The$Pipette/size$button$allows$you$to$select$a$new$pipette$model$
• The$Pipette/ID$button$allows$to$enter$a$numeric$ID$that$will$be$stored$with$the$measured$data$$
• The$Volume$button$allows$the$selection$of$sub-volumes$of$the$pipette$in$the$case$of$variable$volume$
pipettes.$
• The$Settings$panel$can$be$reached$by$touching$on$the$date/&/time/line.$$
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UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&4/9&
Measurement
1. Insert$the$pipette$tip$in$the$center$of$the$tip$port$of$the$instrument.$Hold$the$pipette$vertical$and$firmly/
press/the/tip$into$the$port.$$
23!456758!52!9:;!<5<;99;!95<!!!!!=!!!!!!(liquid'will'permanently'damage'the'instrument)!
2. When$the$tip/is/well/inserted/on/the/port,$then$press$the$Start$button,$without$moving$the$pipette.$
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3. Just$after$the$“ready”$message,$quickly$Press$on$the$pipette$piston,$while$firmly$keeping$the$tip$inserted$in$
the$port.$The$displacement$of$the$piston$has$to$be$done$in$about$1$to$2$seconds,$during$the$BLUE/phase/of/
progress/bar.$
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Don't$press$the$piston$beyond$the$first/stop$
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4. The$progress/bar$at$the$bottom$indicates$that$the$instrument$is$measuring.$The/pipette/and/the/piston/
should/not/move/when/the/progress/bar/is/RED.$$
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5. When$release$display$appears,$move$the$piston$up$again,$without$removing$the$pipette$form$the$port.$
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UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&5/9&
Results panel
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The$leak$rate$is$expressed$in$volume$loss$per$second.$$
A$leak$above$0.5%/sec$of$test$volume$indicates$a$leaky$pipette,$or$leaky$tip$connection$(or$a$bad$insertion$of$
the$tip$into$ATMOS$port).$
The$END$button$will$stop$the$series$and$display$statistics$(only$after$3$measurements).$Usually,$3$
measurements$are$enough$to$get$a$good$estimate$of$pipette$performance.$
The$reasons$for$bad$reading$can$be:$
• Bad$insertion$of$the$tip$into$the$port$
• User$not$following$instructions$during$the$calibration$process$
• A$totally$nonfunctional$pipette$
• A$very$leaky$pipette$or$tip$connection.$
Statistics panel
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The$New/measurement$button$will$go$back$to$main$panel.$

UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&6/9&
Settings panel
The$Settings$panel$can$be$reached$by$touching$on$the$date/&/time/line.$$
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Read ATMOS program
Read$ATMOS$is$a$program$which$allows$reading$data$from$ATMOS$instrument$via$USB$cable$connected$to$a$
computer.$
After$downloading$ReadATMOS$to$your$computer,$connect$ATMOS$to$USB$port$and$run$the$program.$
First$click$the$button$“update$ports”$in$order$to$connect$to$ATMOS.$Select$of$top$left$menu$the$port$which$will$
appear$as$/dev/cu.usbserial-2140$,$or$similar.$
Then$click$“Read$ATMOS”$button.$You$will$be$asked$for$a$filename.$
!!!!!!!! ! !!!!! !
The$first$reading$downloads$all$data$from$ATMOS$and$stores$them$in$the$excel$file.$
You$can$then$display$the$data$for$a$specific$Pipette$ID$in$the$Pipette$ID$scrolling$list.$$If$you$have$several$volumes$
from$the$same$ID,$select$a$volume$(otherwise$keep$*)$
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UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&7/9&
ATMOS 1000 Performance
P"="nominal"pipette"volume"
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./)',&(0(##1)
2(3(*'*"4%4'-))
./)3,(+4#4$01)
P=1000$
1000$µl$and$all$sub-volumes$
±5$µl$
±1.5$µl$
P=200$
200$µl$and$all$sub-volumes$
±1$µl$
±0.3$µl$
P=100$
100$µl$and$all$sub-volumes$
±0.6$µl$
±0.15µl$
P=20$
20$µl$and$all$sub-volumes$
±0.2$µl$
±0.05µl$
Technical specs
Dimensions$ $ 12.5$$x$$8.5$$x$$3$cm$
Weight$$ $ 420$g$
Power$supply$ $ 5V$DC,$2A$
Battery$$$2000$mAh,$Li-ion$
Operational$temp.$from$10$°C$to$40°C$
Altitude$ $ from$sea$level$up$to$3500m$
Troubleshooting
Problem/
Possible/cause/
Solution/
No$power$
Low$battery$
Connect$to$power$supply$
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Instrument$turned$OFF$after$
unused$period$
Press$main$switch$
Not$reproducible$
measurement$
Bad$manipulation$
Refer$to$user's$manual$for$proper$
operation$
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Dirty$or$damaged$tip$port$
Clean$or$replace$tip$port$joint$(no$liquid$!)$
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Very$bad$pipette$
Try$another$pipette$
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Warranty
UniPix$shall$not$be$retained$liable$for$any$consequences$of$improper$ handling,$use,$servicing,$operating$or$
unauthorized$repairs$of$the$instrument$or$the$consequences$of$normal$wear$and$tear$as$well$as$the$failure$to$
follow$the$instructions$of$the$operating$manual.$UniPix$shall$not$liable$for$damage$resulting$from$any$actions$
other$than$those$described$in$the$operating$manual$or$following$the$use$of$non-original$replacement$parts.$
Disposal
Storage$batteries$ and$ electronic$ devices$ must$ be$ disposed$ of$ separately$ from$ household$ trash$ (mixed$
municipal$waste)$at$the$end$of$their$service$life.$
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UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&8/9&
Annex 1: Printer (optional)
The$mini$Bluetooth$thermal$printer$allows$direct$printing$of$ATMOS$test$results.$
It$automatically$connects$to$ATMOS$when$an$instrument$is$detected.$
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• Turn$the$printer$ON$before$turning$ATMOS$ON$
• When$the$printer$is$connected,$the$message$"ATMOS"$is$printed$on$the$paper$
When$using$the$printer,$the$button$PRINT$appears$on$the$statistics$panel.$Pressing$PRINT/will$also$save$the$
data$in$ATMOS$memory$
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Changing$the$paper:$
• Open$the$lid$
• Place$a$58mm$thermal$paper$roll$in$the$right$orientation$
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UniPix&&&&&Atmos&ATMOS&1000&Operation&manual-B& page&9/9&
Annex 2: Interfacing ATMOS
ATMOS$was$conceived$to$allow$transfer$of$the$captured$results$to$any$computer$(Window,$Mac,$Linux)$using$
the$appropriate$software.$ATMOS$USB$is$connected$to$the$industry$standard$CP2102$from$Silicon$labs.$
Connection$is$read$as$a$serial$port$and$communication$is$done$at$115200$bauds$using$the$standard$ASCII$code.$
Commands$are$sent$as$a$single$ASCII$character$and$will$be$interpreted$by$the$ATMOS$internal$parser.$Upon$
reception$of$a$meaningful$command$the$device$will$issue$a$reply$followed$by$<CR/LF>$
Command/summary:/
The$first$set$of$commands$is$for$downloading$measurement$data$from$Atmos$:$
l$Atmos$sends$a$string$of$characters$listing$all$measurements$data$stored$in$the$memory$
w$Erase$all$data$stored$in$ATMOS$memory$
h$Help$command:$this$will$send$the$list$of$all$available$commands$
This$set$of$commands$is$for$remote$controlling$Atmos$for$operation$in$a$robotic$platform$:$
a$After$receiving$this$command,$Atmos$will$start$a$calibration$measurement$sequence$with$the$stored$
sequence$parameters.$The$sequence$parameters$can$be$changed$with$the$commands$below.$
c$Enter$the$calibration$mode.$
Soon$after$issuing$the$“c”$command,$Atmos$will$send$the$character$“<”$indicating$that$it$is$ready$to$read$
the$ pressure$ increase.$ The$ calibration$ process$ takes$ place$ and$ when$ completed$ Atmos$ send$ the$
character$“>”$indicating$that$the$pressure$can$be$released.$
t$After$receiving$this$command,$Atmos$will$read$a$string$of$character$terminated$by$<CR/LF>$used$to$set$
the$timing$of$the$calibration$sequence$(values$should$be$between$2-10)$
v$Set$the$nominal$displacement$volume$(in$microliters)$that$is$planned$to$be$tested.$Enter$a$valid$integer$
followed$by$<CR/LF>$
p$After$receiving$this$command,$Atmos$will$read$a$string$of$character$terminated$by$<CR/LF>$used$for$
the$identification$number$(max.$8$digits)$of$the$pipette$to$be$calibrated$
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