UniPix ATMOS User manual

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ATMOS
Pipette Accuracy Tester
Operation Manual
www.unipix-atmos.com [e-mail]

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Imprint
Information in this document is subject to change without notice. No part of this document may be reproduced
or transmitted without the express written permission of UniPix GmbH.
While every precaution has been taken in the preparation of this document, the publisher and the author
assume no responsibility for errors or missions, or for damages resulting from the use of information contained
in this document or from the use of programs and source code that may accompany it.
In no event shall the publisher and the author be liable for any loss of profit or any other commercial damage
caused or alleged to have been caused directly or indirectly by this document.
© 2023 UniPix GmbH. All rights reserved.
Printed: 02.08.2023
UniPix GmbH
Bei den Pferdeställen 3
72072 Tübingen
Germany
Phone [phone]
[e-mail]
www.unipix-atmos.com

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Contents
Imprint .................................................................................................................................................................. 2
Contents ............................................................................................................................................................... 3
General Information............................................................................................................................................. 5
About ATMOS................................................................................................................................................... 5
Using ATMOS........................................................................................................................................................ 6
Turning on ATMOS ........................................................................................................................................... 6
ATMOS Functions ................................................................................................................................................. 7
Accuracy Function ............................................................................................................................................ 7
Pipette Size Selection Screen....................................................................................................................... 7
Measurement screen................................................................................................................................... 7
Measurement Procedure............................................................................................................................. 8
Results Screen............................................................................................................................................ 10
Statistics Screen ......................................................................................................................................... 11
Molarity Function........................................................................................................................................... 12
Dilution Function............................................................................................................................................ 13
Serial Dilution Function.................................................................................................................................. 14
Calculator Function ........................................................................................................................................ 15
Settings Screen............................................................................................................................................... 16
“ReadATMOS” program...................................................................................................................................... 19
Firmware V2023-V3.0 and above................................................................................................................... 19
All other firmware versions............................................................................................................................ 20
ATMOS Technical Specifications......................................................................................................................... 22
Technical Specifications ................................................................................................................................. 22
Troubleshooting ................................................................................................................................................. 23
Further Information............................................................................................................................................ 24
Warranty ........................................................................................................................................................ 24
Disposal .......................................................................................................................................................... 24

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Disclaimer
•The Pipette Accuracy Tester ATMOS is designed for the specific use of testing and calibrating air displacement
laboratory pipettes and no other use is recommended.
•The user must read and understand this manual before operation.
•This instrument should only be used in a laboratory environment.
•Do not open or attempt to repair the instrument without expressed and explicit instructions from UniPix.
•Do not use the instrument in an atmosphere with a danger of explosion.
•Do not allow unauthorized and/or untrained operators to use this device.
•Do not recharge the instrument in a liquid or humid environment.
•Any misuse will be the sole responsibility of the user/owner and UniPix assumes no implied or inferred liability
for direct or consequential damages from this instrument if it is operated or used in any way other than for
which it was designed.

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General Information
About ATMOS
The UniPix ATMOS Pipette Accuracy Tester is an instrument for precise measurement of air displacement pipettes,
without need of liquid. It is based on comparison with a calibrated internal reference cavity in the instrument. It
also measures the leak rate of pipettes.
Never use the instrument with liquid in the pipette tip.
This will permanently damage the instrument (no repair is possible)!
Allow the internal temperature to stabilize during at least 30 minutes after
unpacking or moving the instrument from another room.
•It is recommended to verify that the instrument battery is well charged or to use the instrument with the
external power supply connected via the USB cable.
•The instrument is powered by an internal Lithium-Polymer battery which allows continuous operation for
more than 3 hours and several weeks with short daily testing sessions.

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Using ATMOS
Turning on ATMOS
•Press the main switch on the front of the instrument to power on.
•After powering on, the instrument runs a self-test procedure for about 10 seconds.
•The home screen then shows all available functions:
oAccuracy: Precise measurement of air displacement pipettes,
in absence of liquid.
oMolarity: Calculator for molar concentration for your
customizable values.
oDilution: Calculator for dilution of your solution.
oSerial dilution: Calculator for serial dilution by pre-defined or
customized factors.
oCalculator: Standard calculator.
oSettings (gear symbol): See/change basic settings like
version/serial number, date/time.
•Select the function you want to use by touching the respective button on the screen. For detailed
description on each function, please see below.
•When not operated, the instrument automatically turns OFF after 5 minutes.
•To shut down manually, press the main switch on the front of the instrument.

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ATMOS Functions
The ATMOS device offers various functions for daily laboratory work.
Apart from the pipette accuracy measurement, it can also support the user with molarity/dilution/serial
dilution calculations and offers a standard calculator.
All functions are described below.
Accuracy Function
The accuracy function will measure the accuracy of your pipette.
Click the button “Accuracy” on the main screen.
Pipette Size Selection Screen
Select the pipette size you are testing:
Measurement screen
After pipette selection or after measurements, you will see the measurement screen.
Select the nominal
volume of the
pipette
Battery status (it is
recommended to keep
ATMOS in charge when
not in use)
Go to settings
screen
Return to main screen

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From this screen, you can reach the following functions:
•The Start button will initiate the measurement procedure. Please insert the tip (WITHOUT LIQUID) and be
ready to actuate the piston BEFORE touching the button.
•The Pipette size button allows you to select a new pipette model. It will bring you back to the selection
screen.
•The Pipette ID button allows you to enter a numeric ID that will be stored with the measured data.
•The Volume button allows the selection of sub-volumes of the pipette in the case of variable volume
pipettes.
Measurement Procedure
1. Insert the pipette tip firmly in the center port. Hold the pipette vertically and steady.
NO LIQUID IN THE PIPETTE TIP! (liquid will permanently damage the instrument)
2. Press the Start button, without moving the pipette.
Start the
measurement
(insert tip BEFORE
pressing “Start”)
Selected volume (for
variable volume
pipettes)
Pipette nominal
volume. Click to change
volume and return to
previous screen.
Date & time (touching on
date & time line will bring
you to the settings screen)
Pipette ID (touch inside
the frame to set a new
pipette ID)

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3. Push the piston to the first stop, while firmly keeping the tip inserted in the port. Movement should be done
during the BLUE phase of progress bar.
4. The progress bar at the bottom indicates that the instrument is measuring. The pipette and the piston should
be kept steady when the progress bar is RED.
5. Release the piston, when the progress bar is GREEN.

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Results Screen
1-channel version
The result screen shows the following data:
Nb: Measurement number. Up to 10 measurements can be shown.
RESULT: Measured volume displacement of each measurement.
Leak rate:Expressed in volume loss per second. A leak above 0.5%/sec of test volume indicates a leaky pipette, or
leaky tip connection (or a bad insertion of the tip into ATMOS port).
The following actions are possible:
SKIP: This will remove the current measurement. It will not be stored in the memory.
NEXT: This will start the next measurement. The current measurement will be stored in the memory.
END: This will stop the series and display statistics (possible only after 3 measurements). Usually, 3 measurements
are enough to get a good estimate of pipette performance.
Leak rate (in volume
loss per second)
Measurement
number
End: Stop the series
(current data will be
stored. Average value
and STDEV will be
displayed)
Next: Start the next
measurement (current
data will be stored)
Skip: This will remove
the current
measurement (data will
not be stored)
Measured volume
displacement
List of previous
measurements
(up to 10 values)