VWR avantor 710-2126 User manual

Instruction manual
VWR®Media Pump
EU cat. no 710-2126
___
Version 01 Issue 06/2021
For manuals in other languages,
please visit vwr.com

2vwr.com I Instruction manual VWR®Media Pump
Legal address of Manufacturer
Europe
VWR
®
International bv,
Researchpark Haasrode 2020
Geldenaaksebaan 464 B-
3001 Leuven
+3216385011
http://be.vwr.com
UK Importer:
VWR International Ltd
Hunter Boulevard, Magna Park
Lutterworth, Leicestershire, LE17 4XN
http://uk.vwr.com
Country of Origin
France

3vwr.com I Instruction manual VWR®Media Pump
Table of Contents
Warning .........................................................................................................................................................................................................4
Safety Information......................................................................................................................................................................................4
Package Contents ......................................................................................................................................................................................5
Installation.....................................................................................................................................................................................................5
Intended use .................................................................................................................................................................................................6
Product Specifications...............................................................................................................................................................................6
Overview ........................................................................................................................................................................................................ 7
Description of Buttons and Switches .................................................................................................................................................... 7
Operational keys.........................................................................................................................................................................................9
Getting Started ............................................................................................................................................................................................9
Important Guidelines ...............................................................................................................................................................................10
General settings.......................................................................................................................................................................................... 11
Operation .................................................................................................................................................................................................... 12
Calibration .................................................................................................................................................................................................. 15
Troubleshooting........................................................................................................................................................................................ 20
Appendix..................................................................................................................................................................................................... 20
Repair and maintenance of Media Pump ......................................................................................................................................... 21
User replaceable accessories and spare parts ................................................................................................................................22
Technical service.......................................................................................................................................................................................22
Warranty......................................................................................................................................................................................................23
Compliance with local laws and regulations ...................................................................................................................................23
Equipment disposal..................................................................................................................................................................................23
Local VWR offices in Europe and Asia Pacific
.............................................................................................................................24

4vwr.com I Instruction manual VWR®Media Pump
Warning
Powerful, it guarantees from 10 to 550 Rotations Per Minute (RPM). The liquid flow is kept closed and sterile:
tubes, bottle and connectors are autoclavable up to 121°C.
Liquids up to 100°C can be dispensed.
Media Pump can be used with lab temperature from 5 to 30°C.
Media Pump is in compliance with ISO 7218 and FDA-BAM standards. 2% precision can be achieved in many
applications.
Safety Information
Avoiding Electrical Shock
Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
To avoid electrical shock:
The power connection should be made with an undamaged power cord provided with a ground
terminal.

5vwr.com I Instruction manual VWR®Media Pump
1
Package Contents
Description
Quantity
Peristaltic Watson MarlowTM pump head
1
Set of 3.2 mm dispensing assembly (nozzle + GL 45 bottle
connector + filter + tubing + tube weight)
1
Set of power cables (EU, UK, CH)
1
User’s manual
1
Conformity certificate
1
Installation
To use
Media Pump
at its best, we suggest installing it on a stable, flat and rigid surface.
Example with bottle container:
Example with broth bag:
Put the tube into the peristaltic pump (from front to back). Be careful to center it to avoid pinching the tubing
when closing the pump. (BE CAREFUL: carefully read the tubing installation guide provided).
When the pump is opened, you must adjust it according to the inner
diameter of the tubing by moving the rotating button.
Turn the wheel on each side of the pump to get the correct setting
according to the diameter (1.6 / 3.2 / 4.8 / 6.4 / 8 mm) and the
thickness of the tubing (1.6 mm).
Insert the tubing into the pump.
Keep the tubing stretched while you close the pump.
WRONG
RIGHT
The tubing is pinched.
It can be pierced.
The tubing is not pinched.
There is no risk.
2
3
4

6vwr.com I Instruction manual VWR®Media Pump
Connect the power cord, press the “ON” button (at the back of the unit).
Intended use
Media Pump is a programmable peristaltic pump designed for liquid distribution from 50 µL to 99 liters. It
dispenses manually or in serial dispensing agar, diluents or any other liquid. With a precise and repeatable flow,
Media Pump is a great tool for filling tubes, containers, broth bags and Petri dishes preparation.
With a user-friendly interface and great ergonomics, Media Pump guarantees an efficient and precise lab work.
It is also very compact, only 16 cm width, and easily fits under a PSM hood. The equipment is robust with a
stainless steel and heavy-duty polycarbonate top shell, designed to last long.
Product Specifications
Dose volume: 50 μL to 99 L
Dispensing modes : Continuous - Dose - Multi-dose
Multi-dose pause: Adjustable from 0.1 to 99.9 s
Watson Marlow™ pump: Single pump head
Typical precision with 3.2 mm inner diameter tubing: 9 mL : ± 1.8 % - 18 mL : ± 1 % - 225 mL : ± 0.5 %
Delivered with (tubing inner diameter): 3.2 mm dispensing assembly
Flow rates: 1.5 mL/min. to 1.25 L/min.
Pump head rotating speed: 10 to 550 rpm
Tubing inner diameter (in option): 1.6 mm - 3.2 mm - 4.8 mm - 6.4 mm - 8 mm
Tubing wall thickness: 1.6 mm only
Tubings made from: Tygon™ (for pump head) and silicon
Net weight: 5.40 kg
Dimensions (w x d x h) : 16.1 x 27.3 x 20.8 cm
Box (w x d x h) : 50 x 40 x 40 cm
Gross weight: 8.20 kg
Voltage – Frequencies: 100-240 V∼50-60 Hz
Fuses: T 2,5A L 250V 5x20 mm
Power: 50 W
International protection marking: 31
All stainless steel
Warranty: 1 year
In compliance with: ISO 7218 & FDA BAM (Bacteriological Analytical Manual)

7vwr.com I Instruction manual VWR®Media Pump
Overview
Description of Buttons and Switches
Front View of Media Pump
Button and a few other buttons.
The general use of Media Pump is easy. The front panel is composed of a screen, a rotating button
and a few other buttons.
Information and menus appear on-screen. The rotating button at the right side of the screen is the
main control of the Media Pump: it allows you to choose the options and launch the operations
(e.g. dispense liquid, calibrate or set a program).
The other buttons have specific functions. They act as shortcuts for specific actions. For example:
3
6
4
5
2
1

8vwr.com I Instruction manual VWR®Media Pump
1. Fill tubing
2. Start / Stop
3. On / Off
4. Esc.
5. Change settings
6. Main button
Rear View of Media Pump
Menu
A menu is a list of choices appearing on-screen. The user turns the rotating button to look at the menu
and presses the button to validate their choice. If the list is too long to fit on the screen, the list scrolls
as the user turns the button.
Example of the main menu, when the Media Pump is turned on:
The on-screen menu is hierarchical: the user can, when choosing an option in the menu, go to another
menu, called sub-menu. For example, the option “MODIFY PROGS“ of the main menu leads to the
following sub-menu:
At any time the “esc.” button allows the user to quit the on-screen menu and go back to the main menu
or the top menu.
RUN PROGRAMS
tube 9 ml
18 ml dishes
multi 9ml
bag
¯Continue
MENU
RUN PROGRAMS
MODIFY PROGRAMS
CALIBRATION
BASIC PUMPING
GENERAL SETTINGS
Title of the menu
List of options
Chosen
option

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Operational keys
Key Functions
Fill tubing: to fill in or fill out the tubing when starting
Start/Stop: to start or stop a dispensing
On/Off key
Change settings: to put the program menu on screen
Escape: to go back on previous menu
Getting Started
Principles of use
Media Pump has 3 dispensing modes: continuous, dose and multi-dose.
Continuous mode:
In this mode, the user controls (by hand or by foot) the starting and stopping moments of the pump. The pump
speed controlling the flow is initially programmed by the user.
Dose mode:
In this mode, the user controls (by hand or by foot) the starting moment of the pump. The pump immediately
stops when the programmed volume is dispensed. The pump speed and the volume to distribute are initially
programmed by the user.
Multiple-dose mode:
In this mode, the user controls (by hand or by foot) the starting moment of the pump for the first dose. The pump
automatically stops when the programmed volume is dispensed. Then it automatically dispenses again after a
programmed delay. The user programs the speed of the pump, the volume to dispense, the number of doses and
the delays between initially.
Principles of operations
Media Pump is a peristaltic pump controlled by a microprocessor.
When a dispensing is programmed, the microprocessor controls the pump in movement and stops it when the
pump has done the asked number of rotations.
A calibration operation is necessary to set and memorize the number of necessary rotations.
Concept of program
Media Pump is a programmable equipment.
The user can choose their preferred programs and memorize them.
Each preferred user mode, (for example 9 mL in mono dose at a 3 mL/s flow) is then called a program. The user
can then enter other programs and name them so they can easily distinguish them.
Media Pump is delivered pre-programmed with the following programs:

10 vwr.com I Instruction manual VWR®Media Pump
• tube 9ml: distribution of 9 mL doses
• 18ml dishes: distribution of 18 mL doses
• multi 9ml: multiples 9 mL doses
• bag: distribution of 225 mL doses
• Continue: continuous and fixed flow
Important Guidelines
Advice for the choice of a tubing
Media Pump can be equipped with tubes with various diameters if you have several tubes and wish to change it
according to your application: either specific flow or viscosity.
Please keep in mind that a program is made to function with a predetermined diameter tubing. The flow
distributed by the pump depends on the tubing's diameter and its speed fixed by the program.
During the liquid flow the inner diameter of the tubing is important to get a good precision. The bigger diameter
on the tubing, the faster the flow (mililiters per minute) and less it will be adapted for dispensing of little volumes.
On the side of the Media Pump is engraved 1 diagram. It shows the precision that you can get according to your
volume and the inner diameter of the tubing.
Example, with the Media Pump:
How to read the diagram:
For a given tubing diameter, the curve indicates the guaranteed precision according to the volume of a dose.
Example: in this figure, for a tubing with inner diameter of 4.8 mm (red curve) and a volume of 9 mL, the
guaranteed precision is inferior to 4 % (follow the dots in red). On the contrary, knowing the volume of your dose
(for example 9 mL) and the maximum precision you want (for example 2%) – (follow the dots in black) -, a tubing
with an inner diameter of 3.2 mm is sufficient because its curve goes underneath the intersections (lines in dots).
Note: the lines in black dots are engraved on the side of the Media Pump, but not the red ones.
9 mL = typical volume to dilute a 1mL sample at 1/10th.
18 mL = typical volume to fill a 90 mm Petri dish with agar.
225 mL = typical volume to dilute a 25 g sample at 1/10th.

11 vwr.com I Instruction manual VWR®Media Pump
Programs and calibration
A calibration is necessary for programs distributing doses with a fixed volume. The calibration enables the Media
Pump to measure the real flow with the conditions of the program, to memorize it and to take it into account at
each dose delivered. The flow rate being known, the Media Pump makes the number of rotations necessary for
each dose.
The flow delivered by the pump will indeed depend on the rotation speed; the given rotation speed of a given
pump, will depend upon the diameter of the tubing and the viscosity of the liquid. The flow directly proportionally
depends on the rotation speed but this proportionality depends on the tubing diameter and the viscosity of the
liquid.
Moreover, with the same tubing and liquid, the medium flow can vary according to the volume of a dose,
especially when going from a few microliters to several liters.
As each program has different parameters: volume of a dose, tubing diameter and liquid viscosity; a calibration is
necessary for each program.
General settings
The access to programming functions in the general settings is made by the choice of the
“CONFIGURATION”
option in the main menu or by pressing the “
change settings”
button on the top right of
the rotating button.
Here are the options appearing in the settings menu in the general settings:
Flow direction of the pump
The arrow displayed in front of “
FLOW DIRECTION”
indicates the direction towards which goes the pump
and consequently the direction in which the tubing must be placed. If the arrow indicates a direction from left to
right like this: then the source must be placed on the left side of the pump and the container on the right side.
To change the direction of the pumping, please select the line and then press the rotating button. Get back to the
main screen by pressing the “
esc.”
button.
Note: The direction of the pumping is a general parameter, meaning that when using the
Media Pump
, the
direction will be set by whatever the program you use.
Anti-drop system
The
Anti-drop
option prevents a drop showing up at the end of the tubing at the end of a distribution. Such a
drop can be a trouble if the user shakes the tubing and the drop falls into the container, because it would alter
the precision.
To prevent that a drip appears, the pump goes back and sucks the drop back in the tubing. This operation can
take a fraction of a second after dispensing.
To activate or deactivate the option, select the
“ANTI-DROP”
line and then press the rotating button. Go
back to the main screen by pressing the “
esc.”
button.
Sound
Media Pump
makes a little sound after each operation.
To activate or deactivate the sound, select the line
“SOUND”
and then press the rotating button. Go back to
the main menu by pressing the “
esc.”
button.
CONFIGURATION
FLOW DIRECTION:Ƈ
ANTI-DROP: yes
SOUND: yes
CONTRAST
DATE / TIME
LANGUAGE:english

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Contrast
This option lets you adjust the contrast on the screen. Depending on the brightness of your environment, you may
want to increase or decrease the contrast.
Select the
“CONTRAST”
line and press the rotating button. Set up the contrast by turning the button and
press again to validate.
Date/time
The setting of the date and time guarantees traceability.
To set the date and time, select the line
“DATE/TIME”
and press the rotating button. With the rotating button,
set up the date and press the button. Repeat the operation to set up the number of the month and the year.
Proceed the same way to set up the time and minutes.
Language
Media Pump
displays its information in French or English. To set up the language, select the language line
“LANGUAGE”
and press the rotating button to go to another language.
Operation
Create a program
In the main menu, choose the option “MODIFY PROGRAMS”. On the screen will appear the list of the programs
already created. If you scroll the list, at the end will appear a list of standard names: program 5, program 6, etc…
These are unused programs. If you want to create a new program for your needs, use one of them.
Select it as indicated below and press the rotating button.
Modify a program
From the main menu, select “MODIFY PROGRAMS”. A screen displays the list of names of programs already
created. Select the program you want to edit and press the rotating button.
The screen that appears shows under the name of the program, a list of all its parameters.
Example:
The list of parameters vary depending on how the program works: by dose or continuous. Here is the list of all the
parameters:
• Name of the program
• Distribution mode
• Pump speed
• Tubing inner diameter
• Power of pump motor
NAME: program 6
MODE: doses
SPEED: 200 rpm
TUBE Ø: 3.2 mm
POWER: auto
VOLUME: 9.00 ml
SAVE
MODIFY PROGRAMS
↑
18 ml dishes
multi 9ml
bag
Continue
Program 6
↓
program 7

13 vwr.com I Instruction manual VWR®Media Pump
• Volume of a dose (unless the functioning mode is continuous)
and for the multi-dose only:
• Number of doses
• Delay in-between the doses
Later in the chapter you will find the meaning of each parameter and how to modify it. To generally modify a
parameter, turn the button to select the line where the parameter is displayed and press the button.
When you have finished modifying these parameters, turn the button until you select the word “SAVE” at the
bottom of the screen and press the button.
You can also press the “esc.” button to come back to the main menu.
Rename a program
To change the name of a program, select the line “NAME” and press the rotating button. The cursor will place
itself on the first letter. Then select the letter you wish to change with the rotating button and press it to select.
Example to change from 9mL tubing to 18mL tubing:
Then turn the button to modify the character under the cursor. Typographic characters scroll:
numbers first and then alphabetic letters and punctuation characters. Turn the button until the
character appears under the cursor and press the button to validate. The cursor then goes to the
next letter.
Turn the button again to modify the next letter. Repeat the operation for all the letters you have to
change. For all the letters you do not want to change, when the cursor is on the letter, just press
the button to validate. The cursor goes to the next letter. When you have chosen and validated the
next letter, press twice on “esc.”.
Turn the button to put the cursor on
the number 9 and press on the button.
NOM: tube 9ml
MODE: doses
SPEED: 200 rpm
TUBE Ø: 3.2 mm
Then turn the button to make the
numbers appear up to number 1 and
press on the button to validate.
The cursor will go to the letter m
following the number 1.
NAME: tube 1ml
MODE: doses
SPEED: 200 rpm
TUBE Ø: 3.2 mm
NOM: program 5
MODE: doses
SPEED: 200 rpm
TUBE Ø: 3.2 mm
POWER: auto
VOLUME: 9.00 ml
SAVE

14 vwr.com I Instruction manual VWR®Media Pump
How to delete characters:
When the cursor is on a letter you wish to delete, for example the new name is shorter than the previous one, turn
the rotating button to the left or to the right until the letter disappears. Then press the button to validate the
deletion. The cursor then goes to the next position.
Repeat until everything is erased. When you have erased the last letter, press “esc.” twice.
Distribution mode
Each program must have at least one dispensing mode: dose, multi-dose or continuous.
To change this mode, select the line “MODE” and press the rotating button.
Then, by turning the button, make the display mode you want and validate by pressing the rotating button. The
cursor then goes to the next line (SPEED).
Pump speed
The speed is measured in RPM, that is to say, Revolution Per Minute. The pump speed and inner diameter of the
tubing condition the flow. (The flow also depends upon the viscosity of the liquid.)
To change the speed, select the line “SPEED” and press the rotating button.
Then by turning the button, change the number and validate by pressing the button.
Another method, to change the speed in an experimental mode:
Launch the program. When the pump is working, the speed is displayed on-screen and you can modify with the
rotating button. The last chosen figure is memorized in the program.
Inner diameter of the tubing
You have to enter and memorize this information for each program. It will then be useful to calibrate the pump.
To change this figure, select the line “TUBE” and press the rotating button.
Then by turning the button, change the figure and validate by pressing the button.
Note: after having changed the figure of the tubing’s diameter, it is recommended to make a calibration.
Power of the pump motor
You can choose the maximum power, economy (minimum) or automatic mode. In the automatic mode, the power
of the pump is settled according to the speed and diameter of the tubing.
Volume of a dose
If you have chosen the mode dose OR multi-dose, enter the volume in mL here.
Number of doses
In the multi-dose mode, the Media Pump dispenses several doses consecutively making a pause in between each
one.
If you choose the multi-doses mode, select the line “DOSES” and the number of doses to dispense.
There is no more letters to add: press
twice on the button
esc.
to end.
NAME:tube 18ml
MODE: doses
SPEED: 200 rpm
TUBE Ø: 3.2 mm
There is no more letters to add: press
twice on the button
esc.
to end.
NAME:tube 18ml
MODE: doses
SPEED: 200 rpm
TUBE Ø: 3.2 mm
POWER: auto
VOLUME: 1.57 l
SAVE

15 vwr.com I Instruction manual VWR®Media Pump
Delay between each dose
If you choose the multi-doses mode, select the line “DELAY” and enter the number of seconds between
two doses.
Calibration
When to calibrate?
When you change the program because calibration is made for each program even if the tubing diameter
remains the same.
Each time you put the tubing back in the pump head, because the tubing is inserted differently and can change
flow.
Necessity of a calibration
Dispensing of a dose
See introduction on this subject.
Dispensing in continuous mode
In the continuous mode, the flow appears on-screen. The flow is proportional to the rotation speed of the pump.
The calibration enables you to evaluate this proportionality precisely and put the precise flow on screen.
The operation of calibration is not absolutely necessary: if you fill in the information correctly about the inner
diameter of the tubing, the Media Pump can then figure out the flow for an average viscosity and function
correctly. But to maintain an optimal precision, calibration is recommended.
Principle of operations
The operation consists in pouring a fixed volume of liquid in conditions closest to real conditions, to measure it
and then enter the value of the volume in the machine so that it can calculate the ratio between the volume and
the number of rotations made, that is to say the flow rate.
The method to measure a volume is generally to weigh the poured liquid and convert its weight in volume,
knowing the weight of the liquid.
The conditions that must be met:
• diameter of the tubing: install the tubing shown in the program
• viscosity: pour the appropriate liquid, at the appropriate temperature (not other settings)
• volume: choose a container accordingly to a dose
Procedure
Principle: Tare a container corresponding to the volume of a dose. Fill it with the Media Pump and measure the
volume of the liquid. Then enter the volume in the machine.
In the main menu, choose the option “CALIBRATION” and in the displayed menu, choose the program to
calibrate.
Example:
Follow the instructions on-screen and press the rotating button to go to the next steps.
CALIBRATION
↑
18 ml dishes
multi 9ml
Continue
program 6
↓program 7

16 vwr.com I Instruction manual VWR®Media Pump
At one point, the screen shows:
The button “fill tube” is at the top left side of the screen. Place the nozzle in a “waste” bottle. Press this button if
the tubing is not totally filled with liquid. Keep your finger pressed until the tubing is filled.
Be careful not to fill up the container during the operations!
Then press “START/STOP” (or on the rotating button) to start the pump and press again to stop it.
Finally, following the instructions displayed on the screen, you will successively:
• weigh the dispensed liquid
• enter its density
• enter its weight
The calibration is then done. Media Pump displays the obtained precision on screen. According to the functioning
mode, it shows:
• Continuous mode: precision on the flow measurement
• Doses mode: precision on the volume of a dose
Example:
Test
If you want to test the calibration you just made, turn the button to select “TEST” and validate. Place an empty
container, possibly calibrated beforehand and then press the button. The pump activates itself and dispenses the
theoretical volume of a dose. Check, possibly by weighing, the volume of the poured liquid.
Calculation of accuracy
The accuracy depends on 2 things:
• tubing inner diameter
• dispensed volume during calibration
Tubing inner diameter:
The tubing inner diameter is important in the dose mode. It must be adapted to the volume of the wanted dose:
for a small volume, the tubing must be thin. If you use a large tubing to dispense a small volume, inaccuracies
may increase.
Dispensed volume during calibration:
Theoretically, the flow measurement after calibration is more precise after you have dispensed a large volume
because you would have measured the real flow during a longer time.
However all the phases of the movement of the pump: start, stop and continuous rotation, are also important in
the precision of the volume used. Therefore, it is better that the volume dispensed during calibration be equal to
the volume dispensed during use. That is to say:
• Dose mode: the volume of a dose
• Continuous mode: the volume the most often dispensed
Accuracy displayed by the Media Pump is calculated on the basis of two parameters discussed above (tubing
diameter and volume added).
CALIBRATION
PRESS FILL TUBE
TO AVOID ANY
AIR BUBBLES
ENTER
CALIBRATION
DONE
PRECISION : 0.77%
TEST
E N D

17 vwr.com I Instruction manual VWR®Media Pump
Use a program
To run a program, the main menu, select “LAUNCH PROGRAMS”. A screen displays the list of program names.
Select the one you want with the rotating button.
Immediate launching
To start the program immediately, without prior verifications, press the red button “START/STOP”.
Note: if you have an accessory: gun or pedal (see Accessories chapter), you can use it instead of the
“START/STOP” button.
Start after verifications
When you choose a program, the display parameters of the chosen program appear on screen. You can check
that you have chosen the right program. If your program is in multi-dose, turn the button to scroll the information
on-screen.
Example:
Then press the button to display the last calibration's date.
Example:
Then press the button or on the red “START/STOP” or on an accessory to start the pump.
Suspend an operation
You can temporarily suspend the operation by 3 ways: by pressing the “START/STOP” button, on the rotating
button or on the “esc.” button.
You can start the dispensing again by pressing the “START/STOP” button again or on the rotating button.
Cancel an operation
When the dispensing is suspended, you can then cancel it totally by pressing the “esc.” button.
Change the speed during dispensing
While the pump is running, you can change the speed by turning the rotating button, thereby changing the liquid
flow.
The used speed is memorized in the Media Pump. When you will launch the same program later, the pump will
run at the speed you had chosen.
Be careful, this may change the calibration value.
Dispensing in “dose” mode
During dispensing the Media Pump displays a progress indicator and a reminder of the program name, the dose
volume and the direction and speed of the pump.
NAME: program 6
MODE: doses
SPEED: 200 rpm
TUBE Ø: 3.2 mm
POWER: auto
VOLUME: 1.57 l
ENTER
CALIBRATED ON :
24/02/2012 14:35
START

18 vwr.com I Instruction manual VWR®Media Pump
Example:
At the end of dispensing, the screen displays “DISTRIBUTION PERFORMED”. You can then start the dispensing of
another dose by pressing “START/STOP” or on the rotating button or using the gun or the foot pedal.
During the dispensing and at the end of each dose, the screen displays a count of the number of doses.
Example, after 10 doses:
Press “esc.” to quit the program.
Dispensing in “multi-doses” mode
During dispensing the Media Pump displays a reminder of the name of the program, the dose volume, the number
of doses of the program and the direction and speed of the pump.
It also displays two progress indicators:
• on the number of doses
• on the dose in dispensing
Example:
Note: during dispensing and during the waiting delay between 2 doses, you can temporarily suspend the
operations by pressing the “START/STOP“ button.
At the end of all dispensing, the screen displays nothing special but that the 2 progression indicators are
complete. The Media Pump makes a little "bip" sound if the option “SON” is activated (see chapter “3.6.3 Sound”).
You can then start the dispensing of another series of doses by pressing “START/STOP” or on the rotating button
or by using the gun or the pedal. If not, press “esc.” to quit the program.
Dispensing in continuous mode
During dispensing, the Media Pump displays the name of the program, the inner diameter of the tubing and the
direction and rotation speed. It also displays the flow in milliliters per minute, the large number at the center of
the screen.
tube 9ml
DISTRIBUTION 10
PERFORMED
9.00ml 300 rpm
multi 9ml
DOSE 10/41
DOSE IN PROGRESS
9.00ml 300 rpm
9 doses have already been
dispensed. The 10th one is being
dispensed…
Total number of doses
tube 9ml
DISTRIBUTION 1
IN PROGRESS
9.00ml 300 rpm

19 vwr.com I Instruction manual VWR®Media Pump
Example:
To stop the flow, press the red “START/STOP” button or on the rotating button. If you use an accessory (foot
pedal or handy gun) (see the Accessories chapter), you can use it instead. To quit the program, press “esc.”
Basic pumping
When you just want to dispense a liquid and manually control the process, use the “BASIC PUMPING” function in
the main menu.
To do so, select the option in the main menu and then press the “START/STOP” button or on the rotating button
and then press the same button again. Stop the pump by pressing the “START/STOP” button again or on the
rotating button.
During dispensing, the pump displays the rotation speed of the motor in RPM.
Example:
During dispensing, you can change the rotation speed by turning the button.
Press “esc.” to get back to the main menu.
continue
ml/min
Ø: 3.2mm 400 rpm
789
BASIC PUMPING
rpm
200

20 vwr.com I Instruction manual VWR®Media Pump
Troubleshooting
Review the information in the table below to troubleshoot operating problems.
Problem
Cause
Solution
I have performed
a calibration and
yet the results I
get are out of
my acceptable
precision range.
Inappropriate choice of tubing size
Check that you have chosen the right size of
tubing for the precision you want (see 3.5.1
Programs and tubings)
Tubings are too old
Replace the tubings
Tubings are pinched
Put the tubings in correct position
The setting of the pump is not
correct
Correct the setting of the pumps
The density entered is not correct
Correct the density in the calibration process
(see chapter Calibration)
The pump has been opened
Calibrate after each opening of the pump
Appendix
Using the handy gun (ref. 710-2127)
For highly precise work, you can use the handy gun
How to install the handy gun
Connect the Jack 3.5 mm connector at the back of the unit of the Media Pump.
Insert the stainless steel nozzle in the gun.
Be careful to aim in the slot where you can distinguish white rollers.
To take out the stainless steel nozzle, unclip it by pulling it down.
Be careful not to press too much.
How to install a diluent broth bag
Disconnect the GL45 bottle cap from the tubing.
Instead of GL45 bottle cap place the trocar for broth bags (ref. 710-2111).
Connect the bag using the trocar.
Install the diluent broth bag on the independent stand (ref. 710-2109).
Push
Clips the tubing
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