WPI ISO-COP-2 User manual

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WORLD
PRECISION
INSTRUMENTS
ISO-COP-2
Macrosensor for CO Measurement
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
030316

MACRO SENSORS
SPECIES Carbon
Monoxide Nitric Oxide Hydrogen Peroxide Oxygen Hydrogen Sulde
Order Number ISO-COP-2 ISO-NOP ISO-HPO-2 ISO-OXY-2 ISO-H2S-2
Available Diameters 2 mm 2 mm 2 mm 2 mm 2 mm
Response Time < 10 sec < 5 sec < 5 sec < 10 sec < 5 sec
Detection Limit/Range 10nM to 10µM 1 NM to 40µM* < 100nM to
100µM
0.1%-100% < 5nM-100µM
Sensitivity ~0.5 pA/nM ≤ 2 pA/nM 8 pA/µM 0.3-0.6nA/% 2 pA/nM
Drift <1pA/min <1pA/min 0.1pA/min < 1%/min
Temperature Dependent Yes Yes Yes Yes Yes
Physiological Interference nitric oxide NaNO2(10-6 or
better)
None None None
Replacement Sleeves (pkg
of 4)
#95620 #5436 #600012 #5378 #600016
Filling Solution #95611 #7325 #100042 #7326 #100084
Start-up Kit #95699 #5435 #600011 #5377 #600015
*Higher detection limit available on request — call for custom pricing.
• Real-time detection using electrochemical microsensors
• Measure nitric oxide from < 0.3 nM to 100µM.
• Measure hydrogen peroxide < 10 nM to
100mM
• Measure hydrogen sulde
• Measure glucose
• Measure oxygen from 0.1% to 100%
• Integrated system includes one temperature
sensor, your choice of two additional sensors,
and a start-up kit
• Current measurement range from 300 fA to 10
µA (four ranges) permits wide dynamic range
for detection.
• Wide bandwidth allows recording of fast events
• Isolated architecture allows Lab-Trax interface to simultaneously measure free
radical and independent analog data (i.e., ECG, BP, etc.) data on any channel.
Free Radical Analyzer
Other WPI Favorites

ISO-COP-2
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Copyright © 2016 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 2
Sensor Design............................................................................................................................ 2
Notes and Warnings................................................................................................................. 2
Parts List...................................................................................................................................... 3
Unpacking................................................................................................................................... 3
INSTRUMENT DESCRIPTION ........................................................................................................ 5
Structure of the Sensor........................................................................................................... 5
OPERATING INSTRUCTIONS......................................................................................................... 6
Environmental Inuences ....................................................................................................... 6
Temperature......................................................................................................................... 6
Electrical Interference ...................................................................................................... 6
Setup ........................................................................................................................................... 6
Polarizing the Sensor .............................................................................................................. 7
SENSOR CARE AND MAINTENANCE........................................................................................... 8
Durability and Handling........................................................................................................... 8
Storing the Sensor.................................................................................................................... 8
Cleaning the Membrane.......................................................................................................... 8
Sterilizing the Membrane........................................................................................................ 9
Replacing the Membrane Sleeve .......................................................................................... 9
ACCESSORIES.................................................................................................................................11
TROUBLESHOOTING ...................................................................................................................12
SPECIFICATIONS............................................................................................................................13
INDEX...............................................................................................................................................14
WARRANTY .....................................................................................................................................15
Claims and Returns ................................................................................................................15
Repairs.......................................................................................................................................15

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ISO-COP-2
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ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that
can cause damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that
can cause personal injury or pose a physical threat. Please read these
carefully.
NOTES and TIPS contain helpful information.
Fig. 1—ISO-COP-2 sensor

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INTRODUCTION
The ISO-COP-2 is a carbon monoxide sensor with a replaceable stainless
steel membrane sleeve lled with an electrolyte solution that measures CO in
vivo or in vitro in real time!
Replacement membrane sleeves can be purchased in packages of four (WPI
#95620), along with lling solution (WPI #95611). A startup kit for this sensor
is available and is recommended for the rst purchase of this sensor type
(WPI #95699).
Sensor Design
The ISO-COP-2 is an amperometric sensor designed for use in cell culture
and similar applications.
In principle, CO diuses through the gas-permeable membrane and is then
oxidized to CO2on the working electrode of the sensor. This oxidation
creates a current with a magnitude directly related to the concentration of
CO in solution.
It is designed for use with WPI’s TRB4100 (4-Channel Free Radical Analyzer),
TBR1025 (1-Channel) or the Apollo1000.
Notes and Warnings
WARNING: CARBON MONOXIDE GAS MUST BE HANDLED ONLY IN
A WELL-VENTILATED AREA, TYPICALLY A LABORATORY FUME
HOOD WITH FORCED VENTILATION. THE U.S. OCCUPATIONAL
SAFETY AND HEALTH ADMINISTRATION HAS SET A TIME-
WEIGHTED AVERAGE (8-HOUR) (TWA) PERMISSIBLE EXPOSURE
LIMIT (PEL) OF CARBON MONOXIDE AS 50PPM. THE ACGIH
THRESHOLD LIMIT VALUE (TLV) FOR CARBON MONOXIDE IS 25PPM TWA. THAT
IS TO SAY, 50PPM IS CITED AS THE MAXIMUM CONCENTRATION TO WHICH
WORKERS MAY BE CONTINUALLY EXPOSED. OVEREXPOSURE MAY RESULT IN
HEADACHES, DIZZINESS, CONVULSIONS, LOSS OF CONSCIOUSNESS AND
DEATH. CARBON MONOXIDE IS ALSO EXTREMELY FLAMMABLE. IT IS
THEREFORE CRITICAL THAT THE PERSONNEL HANDLING THE GAS BE
THOROUGHLY FAMILIAR WITH THE MATERIAL SAFETY DATA SHEET (MSDS) AND
PROPER HANDLING PROCEDURES. THE PRECAUTIONS RECOMMENDED BY THE
GAS MANUFACTURER MUST BE FOLLOWED.
CAUTION: DO NOT EXPOSE SENSOR TO ORGANIC SOLVENTS.

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CAUTION: Carefully read the “Probe Unpacking” instructions (found in the
sealed sensor case) before handling the sensor.
NOTE: The sensor must be polarized for at least 12 hours in 0.1M PBS buer
solution prior to use.
NOTE: The sensor membrane and membrane adhesive are extremely
delicate. Improper handling will lead to damage of the sensor. The ISO-COP-2
is designed for use in liquids.
NOTE: For optimal use of each sensor and sensor membrane sleeve, the
sensor must be stored properly. See “Storing the Sensor” on page 8.
Parts List
After unpacking, verify that there is no visible damage to the sensor. Verify
that all items are included:
(1) ISO-COP-2 CO sensor
(1) Probe Unpacking Instructions (Read this before handling the probe.)
(1) ISO-COP-2 Data sheet
(1) Spare membrane sleeve
(1) 1 cc Syringe
(1) MF28G67 MicroFil
(1) 95611 10mL vial of lling solution
(1) Instruction Manual
Unpacking
Upon receipt of this sensor, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons
should be noted on the delivery receipt before signing. Concealed damage
should be reported at once to the carrier and an inspection requested.
Please read the section entitled “Claims and Returns” on page 15 of this
manual. Please contact WPI Customer Service if any parts are missing at
941.371.1003 or [email protected].
The ISO-COP-2 sensor is shipped in a sealed, rigid plastic, hinged box with
foam padding to avoid damage to your sensor during shipment. The tip of
the sensor is protected by inserting it into a small, plastic vial containing
deionized water (DIW) to keep the membrane from drying out.
To open the package, carefully cut the seals on either side of the sensor box.
Inside the box, on top of the sensor, are the important documents to read

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before handling the sensor, including the instructions for removal of the
sensor tip from the protection vial. Keep the sensor tip in this vial until the
sensor is ready for use.
KEEP THE SENSOR STORAGE BOX and all the documentation in a safe place.
Use of the sensor should begin within 30 days of receipt.
Returns: Do not return any goods to WPI without obtaining prior approval
(RMA # required) and instructions from WPI’s Returns Department. Goods
returned (unauthorized) by collect freight may be refused. If a return
shipment is necessary, use the original container, if possible. If the original
container is not available, use a suitable substitute that is rigid and of
adequate size. For further details, please read the section entitled “Claims
and Returns” on page 15 of this manual.

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INSTRUMENT DESCRIPTION
Structure of the Sensor
The basic structure of the ISO-COP-2 sensor is shown below (Fig. 2).
Fig. 2—ISO-COP-2 Sensor assembly
Gas permeable, polymeric membrane covering the end of the stainless
sleeve to separate it from the external environment
Disposable, protective stainless steel sleeve (WPI#95620) that houses the
sensitive electrode pair. The sleeve is anged to properly connect with the
locking cap. It must contain fresh electrolyte (WPI#95611).
Internal CO-sensing pair of working and counter (reference) electrodes
Probe handle
Locking cap attaches the sleeve to the probe handle
When the sensor is fully assembled (with locking cap and sleeve in place)
the internal electrode should press gently against the polymeric membrane,
which will be slightly stretched. This ensures that the electrolyte diusion is as
thin as possible, minimizing sensor response time.
NOTE: Once a membrane is stretched it is permanently deformed and
cannot be reused if the sleeve is removed from the electrode.
Additional membrane sleeves are available in packages of 4 (WPI # 95620).
The ISO-COP-2 start-up kit (WPI #95699) also includes replacement
membrane sleeves, along with all the accessories to ll them properly with
electrolyte solution.

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OPERATING INSTRUCTIONS
Environmental Inuences
There are two environmental parameters to which CO sensors are quite
sensitive: temperature and electrical interference.
Temperature
The background current (and to a lesser degree) the selectivity of the CO
sensor is aected by temperature. This is due to the eects of temperature
on the partial pressure of dissolved CO gas in liquid samples, on the
permeability of the membrane and on the conductivities of various sensor
components. It is recommended that the calibration procedure be performed
at the same temperature as the experiment and that temperature be held
constant during CO measurement.
Electrical Interference
External, electrical noise sources (like uorescent lights, MRI machines,
electric motors, computers, pumps and other electrical instruments)
may couple into the sensor signal path electromagnetically and impose
undesirable signals in the output record. The magnitude of this external noise
depends on the environment of the laboratory. If the interference introduced
by the electrical signals in the environment is large, the rst step towards
eliminating it is to ground and shield the system properly.
TIP: Refer to your free radical analyzer manual for proper grounding
and shielding techniques. (In the TBR4100 or Apollo1000 manuals, see
“Grounding and Noise Concerns” in the Operating Instructions section.)
Setup
The CO electrolyte lling solution (WPI #95611) must be made fresh when
you are ready to use the sensor, so the ISO-COP-2 sensor is shipped with
a dry membrane sleeve. Before using it, you must ll the sleeve with the CO
electrolyte lling solution. Use the membrane sleeve that was shipped on
your sensor and follow the instructions for replacing a membrane sleeve
(page 9) to setup your sensor for its polarization, calibration and rst use.
NOTE: Since the membrane sleeve that was on the sensor during shipping
was dry, the membrane was not damaged by placing it on the sensor. That
membrane sleeve is still good to use.