YL Instruments YL9100 HPLC User manual

YL9100 HPLC SYSTEM
YL9101 VACUUM DEGASSER
USER MANUAL

Y
L9101
Contents
Chapter 1. Introduction.............................................................................................1
1-1. Specifications ....................................................................................................................................1
Chapter 2. Installation ...............................................................................................2
2-1. Inspection and site preparation.........................................................................................................2
2-2. Connection of power .........................................................................................................................2
2-3. Connection of tubing .........................................................................................................................3
2-3-1. Connecting of mobile phase solvent ...............................................................................3
2-3-2. Connecting of Solvent delivery pump..............................................................................3
Chapter 3. Operation.................................................................................................4
3-1. Degassing of the solvent...................................................................................................................4
3-2. Operating of solvent delivery pump...................................................................................................4
3-3. Replacing solvents ............................................................................................................................4
3-4. Keeping method after use.................................................................................................................4
Chapter 4. Maintenance ............................................................................................5
4-1. Filtration of solvent............................................................................................................................5
4-2. Caution..............................................................................................................................................5
4-3. Troubleshooting.................................................................................................................................5
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YL9101 Vacuum Degasser
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Chapter 1. Introduction
It is not easy but important to select and handle mobile phase solvent in HPLC(High Performance
Liquid Chromatography). YL9101 Vacuum Degasser is connected a high productive vacuum Degassing
module which is built in the mixer and it is equipped with a precise 4-way valve module to provide
optimum solvent degassing to HPLC user.
Degassing of mobile phase solvent
Solvent is degassed when passing through the vacuum degasser, solvent preparation is greatly
simplified and the degassed solvent is ready to use for analysis. With an in-line vacuum degasser, you can
realize excellent environment for HPLC analysis.
YL9101 Vacuum Degasser consists of four channels for degassing and each channel removes
efficiently air and oxygen in the solvent. Also solvents, which were degassed by each channel, were mixed
up with 4-way valve and then were degassed one time again. Therefore the YL9101 Vacuum Degasser
provides the best optimized solvent.
1-1. Specifications
1) Number of channel: 4 CHs
2) Maximum flow rate : 10ml/min per channel
0-2.0ml/min per channel for 70% Gas Removed from Methanol
3) Internal volume per channel : 925ul per channel
®AF, PEEK and Glass-filled PTFE
4) Materials in contact with solvent : Teflon
5) Safety & maintenance : Error detection
6) Dimensions : 385 X 80 X 565mm (width X height X depth)
7) Line Voltage : 110 or 220VAC, ±10%, automatic voltage selection
8) Line frequency : 50/60Hz, ±5%
9) Power consumption : 20W
1

YL9101 Vacuum Degasser
Chapter 2. Installation
2-1. Inspection and site preparation
Site requirement of YL9101 Vacuum Degasser
1) Room with 20℃temperature with variation ± 5℃with and 60% humidity
2) Where no direct and straight sunlight
3) Plain floor without carpet
4) Where having spare space of 20cm or more
5) Where there are 10% or less voltage change, no frequency change and 100Ωor less grounding
point
6) Where there is no generation of corrosive gas and ventilation is well done
7) Where stable power of 110 or 220V is supplied, ±10%
8) Where not receive electromagnetic induction from large transformer, high frequency heater, UPS,
etc.
9) Within 2500 m above sea level(storage within 4600m)
Please check the following before you install the system.
1) Keep the ventilation as normal state.
2) Install on the stable place. Avoid the places as like near to air conditioner and heater, direct sun
light, near to window.
3) Keep the place without dust and vibration.
4) Maintain voltage variation within 5% of proper voltage.
5) Avoid high frequency or strong magnetic field environment.
6) Avoid from the source of fire(spark, flame).
7) Keep the proper ground for electricity.
8) Check the place of water supply for emergency.
Caution ! : Keep distance with CRT at least 50cm.
2-2. Connection of power
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YL9101 Vacuum Degasser
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L9101
Confirm supplying voltage, electric power, socket, and type of connector are correct. Before connect
power cable on the pump, please turn off the power switch of YL9101 Vacuum Degasser.
Warning ! : The electric power should be matched with description on the rear side of instrument. If not,
the instrument can be damaged or it can be a reason of fire.
Caution ! : Confirm the ground is correct. Water pipe is not recommended in case of non-metal pipe.
Gas pipe should not use because of safety. If the ground is not correct, the electric shock
can be happened.
2-3. Connection of tubing
2-3-1. Connecting of mobile phase solvent
YL9101 Vacuum Degasser can connect 4 kinds of solvent. If you do not use less than 4 solvents, you
must plug up the solvent port, which is not used. Open a dust cover in the front, you will see 8 ports. 4
ports left are for input and right 4 ports are for output. Use a plug screw supplied to plug up un-used
ports.
There is solvent reservoir in the upper part of YL9101 Vacuum Degasser. Place solvent bottles on the
reservoir and connect input tubes. All input tubes have in-line 10 μm solvent filter in the end to prevent
minute particles from entering into pump.
2-3-2. Connecting of Solvent delivery pump
Connect the mixed tube to the input of Gradient Pump. The mixed tube is located in right middle of
YL9101 Vacuum Degasser. Open prim/purge valve by turning in counter-clock wise and setting mixing
rate as 100% for each solvent and pull out solvent by a syringe. Check if all tubes are filled with solvent.
Do not pull out quickly nor strongly, it will damage the in-line vacuum degasser. Pull out slowly until when
tubes are will with solvent.
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YL9101 Vacuum Degasser
Chapter 3. Operation
There are four LEDs in front of YL9101 Vacuum Degasser.
POWER LED turns ON if main power turns on
VACUUM LED turns ON during vacuuming
STATUS LED turns ON if there is error during vacuum degassing
3-1. Degassing of the solvent
Power on YL9101 Vacuum Degasser by pressing power switch, which is located in the front. When you
power on, check if pump is stopped, otherwise stop pump before turning on YL9101 Vacuum Degasser.
YL9101 Vacuum Degasser will start immediately to degas solvent. If normally operational, green LED
(VACUUM) located far left is on.
3-2. Operating of solvent delivery pump
Set mixing rate constant for all solvents and start pump at flow rate of 1.0 ml/min. Air bubble will be
coming when start. To remove the bubble, open the prime/purge valve and pull out air bubble by syringe
slowly until the bubbles are removed. After remove, close the valve for normal use of pump.
3-3. Replacing solvents
When necessary to replace solvents during analysis, stop pump and replace solvents and re-start
pump. But do not turn off YL9101 Vacuum Degasser. If you want to change solvent filled inside if YL9101
Vacuum Degasser completely, set mixing rate at 100% for the solvent, pull out more than 15 ml solvent
by syringe or start up pump and wait until solvent is completely changed.
3-4. Keeping method after use
If you do not use YL9101 Vacuum Degasser more than 1 day, use ultra pure water and flow into
vacuum degasser and rinse with alcohol completely and keep it. Specially when use with buffering
solution, be sure to rinse YL9101 Vacuum Degasser, otherwise precipitation can be occurred. In the case
YL9101 Vacuum Degasser is kept lower than 0℃, dry completely after rinse.
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YL9101 Vacuum Degasser
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Chapter 4. Maintenance
4-1. Filtration of solvent
Be sure to filter solvent by <5 μm when specially use buffering solution. Keep filtered solvent in the bottle,
which is cleared from minute particles. When use filtered solvent, which kept more than 1 week, please
filter again before use.
4-2. Caution
1) Do not pressurize the vacuum degasser module. More than 120 kPa pressure will damage degassing
membrane inside. When you put solvent bottles 2 m higher than degasser, the pressure is
approximately 120 kPa. So place the bottles less than 2 m lower.
2) Be sure to prevent from any contamination when you connect or disconnect tubes. Entering fine dust
will reduce degassing efficiency greatly.
3) If you want to increase degassing efficiency, make parallel connection of input port to output port. Do
not connect it by serial way.
4) Can not use YL9101 Vacuum Degasser for output of solvent delivery pump.
4-3. Troubleshooting
1) Error and malfunctioning
Errors of YL9101 Vacuum Degasser are occurred when ……
a) Not approach normal vacuum state 15 minutes after power on.
b) Rapid pressure drops by solvent leak during normal operation.
When above errors occurred, degasser will turn red LED. Open above solvent reservoir and check if
vacuum lines are installed correctly. If you cannot find any thing wrong, please contact our representative
or us. In order to re-start, turn off and wait for more than 5 seconds and turn on.
2) Continuous air bubbling
If air bubbles are continuously happened after turning YL9101 Vacuum Degasser on more than 1 hour,
check if input and output port and valve fitting are completed connected. Do not tighten the screw. Fitting
and joint part can be damaged. If the problem is not solved, please contact our representative or us.
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YL9100 HPLC SYSTEM
YL9110 QUATERNARY
SOLVENT DELIVERY PUMP
USER MANUAL

YL9110 Quaternary Pump
Contents
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L9110
Chapter 1. Introduction.............................................................................................1
1-1. Specifications ....................................................................................................................................2
Chapter 2. Configuration and Principle...................................................................3
2-1. Configuration of flow path..................................................................................................................3
2-2. Operation...........................................................................................................................................5
2-3. Compressibility compensation ..........................................................................................................5
2-4. Solenoid valve control.......................................................................................................................6
2-5. Gradient curve...................................................................................................................................7
2-6. Rinse port..........................................................................................................................................7
Chapter 3. Installation ...............................................................................................8
3-1. Inspection and site preparation.........................................................................................................8
3-2. Connection of power .........................................................................................................................9
3-3. Connection of tubing .........................................................................................................................9
3-4. Connection of remote cable ............................................................................................................12
3-5. Connection of communication cable...............................................................................................14
Chapter 4. Operation...............................................................................................15
4-1. Before Start .....................................................................................................................................15
4-2. Mobile phase filter and bottle..........................................................................................................16
4-3. Preparation of solvent .....................................................................................................................16
4-3-1.Degassing.......................................................................................................................16
4-3-2. Filtration.........................................................................................................................17
4-3-3. Solvent effect on the instrument....................................................................................17
4-3-4. Measures when not uses for long time..........................................................................18
4-4. YL-Clarity Chromatograph software................................................................................................18
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YL9110 Quaternary Pump
4-4-1.Installation of pump ........................................................................................................18
4-4-2. Device Monitor...............................................................................................................20
4-4-3. Method Setup ................................................................................................................21
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4-4-4. Error message ............................................................................................................22
Chapter 5. Maintenance...............................................................................................................23
5-1. Caution............................................................................................................................................23
5-2. Replacement of high pressure seal.................................................................................................23
5-3. Replacement of plunger..................................................................................................................25
5-4. Replacement of check valve cartridge............................................................................................26
5-5. Replacement of low pressure seal..................................................................................................26
5-6. Cleaning of flow path within pump ..................................................................................................26
5-7. Supply of lubricant...........................................................................................................................27
5-8. Replacement of solvent filter and in-line filter.................................................................................27
5-9. After use system..............................................................................................................................29
5-10. Troubleshooting.............................................................................................................................30
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YL9110 Quaternary Pump
Figure Contents
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L9110
[Fig. 1] Flow diagram of YL9110 pump.....................................................................................................3
[Fig. 2] Delivery mechanism of YL9110 quaternary pump........................................................................6
[Fig. 3] Compensation of solenoid valve time gap ...................................................................................6
[Fig. 4] Gradient curve..............................................................................................................................7
[Fig. 5] Fitting for 1/16” OD tubing..........................................................................................................10
[Fig. 6] Connection between YL9110 quaternary pump and YL9100 HPLC system..............................11
[Fig. 7] Tubing connection of rinse port..................................................................................................11
[Fig. 8] Connection of remote cable between YL9110 pump and injector..............................................12
[Fig. 9] Pin configuration of each terminal..............................................................................................12
[Fig. 10] Diagram of Remote and Marker input......................................................................................13
[Fig. 11] Diagram of Remote and Marker output....................................................................................13
[Fig. 12] Diagram of solenoid output ......................................................................................................13
[Fig. 13] Connection of communication cable ........................................................................................14
[Fig. 14] Replacement of high pressure seal..........................................................................................24
[Fig. 15] Replacement of in-line filter......................................................................................................29
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YL9110 Quaternary Pump
Chapter 1. Introduction
Y
L9110
Young Lin YL9110 Solvent delivery pump is a pump for High performance liquid chromatograph. It
provides high performance and functions as a HPLC pump, and controlled by software.
YL9110 quaternary pump uses specially designed cam and pulse damper for stable solvent delivery and
has a compressibility compensation function for accurate and precise solvent delivery.
And the pump has a automatic rinsing function to prolong the life time of pump head. Using UHMWPE
seal, it provides extended life time of high pressure seal even though use buffer solution as a mobile
phase. On the outlet of pump, there is a in-line filter to prevent small particles come into the column and
also protect column by the pressure limitation setup. YL9110 quaternary pump consists of two
independent dual carrier assembly to provides high pressure gradient operation. YL9110 quaternary pump
provides auto prime/purge function for easy operation and fast exchanging of solvent. This instrument
manual includes basic principle, installation and operation method, and troubleshooting to use YL9110
quaternary pump properly.
1

YL9110 Quaternary Pump
1-1. Specifications
1) Operating principle : Parallel dual-plunger pump, low pressure gradient
2) Compressibility compensation : Automatic
3) Flow range : 0.001-10ml/min
4) Flow rate accuracy : ≤±1% at 1ml/min
5) Flow rate precision : 0.1% RSD at 1ml/min
6) Maximum pressure : 6000 psi
7) Operating range : 0-6000 psi up to 5ml/min
8) Operating range : 0-3000 psi up to 10ml/min
9) Pressure pulsation : ≤±1% at 1ml/min
10) Composition Precision : <0.1%
11) Composition Accuracy : <0.5%
12) Semi-Auto prime/purge
13) Communications : LAN
14) Safety & maintenance : Leak detection, Diagnostics, Error detection
15) Dimensions : 385 X 160 X 565 mm (width X height X depth)
16) Line Voltage : 110 or 220 VAC, ±10%
17) Line frequency : 50/60Hz, ±5%
18) Power consumption : 70W
[Optional specification]
Head type Narrow Bore Analytical Semi prep
Head volume(μl/each) 25 64 144
Flow range(ml/min) 0.001~5 0.001~10 0.01~50
Flow range(ml/min) 0.005~5 0.01~10 0.05~25
Pressure(psi) 5000psi 6000psi 3500psi
In-line filter
(micron) 2 2 10
Material
Zirconium, Ruby,
PEEK
UHMWPE, PTFE
SS316, Zirconium,
PTFE, Sapphire,
UHMWPE
SUS316, Sapphire,
Ruby, UHMWPE, PTFE
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YL9110 Quaternary Pump
Chapter 2. Configuration and Principle
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2-1. Configuration of flow path
YL9110 quaternary pump consists as same with figure 1. It uses Teflon tubing between pump inlet and
inlet check valve, SUS316 or PEEK tubing from outlet check valve.
[Fig. 1] Flow diagram of YL9110 pump
•Solvent Filter
The solvent filter is used for protecting the system from the particles in solvent. This filter removes
particles from solvent to prolong the life time of high pressure seal and prevent damage on the column.
It is recommended to use the solvent filter when you use YL9110 quaternary pump with or without
degassing module. Select proper filter depending on the column and flow rate.
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YL9110 Quaternary Pump
•Pump Head Assembly
Pump head is real working part to deliver solvent by piston movement and check valve. It consists of
plunger, check valve, high pressure seal, low pressure seal and rinsing port. YL9110 quaternary pump
built in automatic rinsing port to clean the head assembly efficiently if uses buffer solution.
•Auto Rinsing Pump
Auto rinsing pump delivers cleaning solvent from the rinsing solvent bottle to the inside of pump head.
YL9110 quaternary pump rinse the system every 3 minutes automatically.
•Prime/purge valve
This valve is used for priming the pump. Fill the solvent inside of tubing from the solvent bottle if you use
the pump for the first time or the tubing lines are empty. Remove a plug on the prime/purge valve and
suck the solvent using syringe, or click “Prime Start” button on the software to operate the micro pump.
•Prime micro pump
Using this pump, the pump circulates rinse solvent into the pump head.
•Solenoid valve
It is mixing valve for low pressure gradient. YL9110 quaternary pump controls this valve for gradient
according to solvent ratio and pump speed.
•Mixer
It is static mixer to improve mixing efficiency of solvent.
•Pulse damper
YL9110 quaternary pump reduces pulse from the cam operation by the diaphragm damper. YL9110
quaternary pump provides constant and pulseless flow using compressibility compensation and pulse
damper, so the detector that affected by flow stability can be used with YL9110 pump.
•Pressure transducer
It checks real time system pressure. The pump uses this pressure to protect system and to operate
compressibility compensation and even compensation. YL9110 quaternary pump uses continuous flow
path type pressure sensor.
•In-line filter
It removes fine particles that are not filtered by solvent filter or made by worn of high pressure seal.
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YL9110 Quaternary Pump
2-2. Operation
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L9110
There are essential parameters on the pump as like flow accuracy, precision, and reproducibility to get
the reliance of analysis data and low detection limit. YL9110 quaternary pump uses high pressure resistant
dual pump head, controls microprocessor to monitor the phase of cam to remove pulse, so fulfill the
necessities of solvent delivery pump.
Diaphragm pulse damper reduces pulse from the cam operation more than 90% between low and high
pressure range by internal elastic body of damper, and also works well as a mixer for gradient elution. The
elastic body of Diaphragm pulse damper improves flow accuracy through compression, expansion
procedure to make variance of kinetic energy by flow constant. The amount of contact solvent with pulse
damper is around 1.5ml at 3000psi, which ensures that flow path is completely cleared away.
YL9110 quaternary pump was designed so that integrated flow rate may realize no-pulse operation
using specially designed cam. However, pulse incapable of being neglected is caused actually due to
compressibility of mobile phase proportional to pressure and elasticity of high pressure seal, so the pump
is controlled in real time so that occurrence of pulse proportional to pressure may be depressed. Control
method of the Pump uses supervision of pressure and control of location simultaneously, so it has
advantage to improve precision and accuracy of flow rate without being affected by range of pressure and
flow rate. There is a part for microprocessor control to realize various function of pump including
stepping motor control. Stepping motor control processor operates motor as a micro step, so can achieve
constant motor speed at low flow rate with low noise.
Operation mechanism is for transmission of kinetic energy from step motor to piston. This mechanism
includes specially designed cam, stepping motor, carrier, carrier housing, and phase sensor.
2-3. Compressibility compensation
Most of pumps for HPLC analysis are used at high pressure. However, pulse occurs in high pressure
due to compressibility of liquid and elasticity ratio of seal, so flow rate is also reduced. Occurrence of pulse
due to this reduces precision and accuracy of pump flow rate, so compensation is necessary for this.
YL9110 quaternary pump monitors actual pressure and calculates compensation value for this;
compressibility compensating operation to control angular velocity of cam with this value reduces
occurrence of pulse flow remarkably as well as improves accuracy of flow rate largely.
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YL9110 Quaternary Pump
Pulsation by compressibility and delay Compensation of pulsation
[Fig. 2] Delivery mechanism of YL9110 quaternary pump
2-4. Solenoid valve control
YL9110 quaternary pump controls open/close signal of solenoid valve to perform low pressure gradient
elution. For accuracy and precision of solvent mixing, YL9110 quaternary pump controls valve opening
and closing time with real time compensation according to time gap of valve cycling and the phase speed
of cam.
[Fig. 3] Compensation of solenoid valve time gap
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YL9110 Quaternary Pump
2-5. Gradient curve
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L9110
Solvent A and solvent B are mixed with flow rate Fb,
Mixed solvent flows to the column with flow rate Fab,
Solvent volume V of solvent A vs Concentration of solvent B
C(%) = 100 x {1 - (Fab-Fb)/V x t}(Fb/Fab-Fb)
The solvent ratio difference between present row and next row increases or decreases as a exponential
curve. In general, the gradient elution is used for separation of duplicated peaks. YL9110 quaternary
pump has a exponential curve program, but use gradient elution by linear curve when it operates with
YLClarity software.
[Fig. 4] Gradient curve
2-6. Rinse port
When using buffer solution, salts are generated on back side of high pressure seal and these deposits
wear pump seal to cause shortening of seal life, which has bad effect on pump.
Rinse port enables to insert proper solvent in back side of high pressure seal to prevent salts from being
deposited and activated. Mixed solution(20% MeOH) of water and methanol is used as cleaning solution,
and life of seal is extended with lubrication action in general analysis..
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YL9110 Quaternary Pump
Chapter 3. Installation
3-1. Inspection and site preparation
YL9110 quaternary Pump is delivered along with the following parts when being shipped. Before
opening transportation package, perform inspection for trace of shock or mistake, and if there is
abnormality, do not open the contents and inform this company of it. And, if contents are opened, perform
inspection for existence of shock in the contents and contact with this company when trace of shock is
found.
The Gradient Pump is a delicate instrument, so use original box and buffer material as far as possible
when re-packing it to transport instrument. If it is impossible to use original box; wrap pump with several
layers of buffer material, and fill the bottom, top and all other sides of pump with buffer material in order to
make pump endure shock or vibration during transportation.
Standard configuration of YL9110 quaternary pump
1) Main body of instrument
2) Power cord and fuse
3) Tubing 60cm,
A. Bio Narrow Bore : ID 0.01” , OD 1/16” PEEK
B. Analytical , Semi-prep : ID 0.02”, OD 1/16” SUS316
4) Installation kit
5) Manual
Site requirement of YL9110 quaternary pump
1) Room with 20℃temperature with variation ± 5℃with and 60% humidity
2) Where no direct and straight sunlight
3) Plain floor without carpet
4) Where having spare space of 20cm or more
5) Where there are 10% or less voltage change, no frequency change and 100Ωor less grounding
point
6) Where there is no generation of corrosive gas and ventilation is well done
7) Where stable power of 110 or 220V is supplied, ±10%
8) Where not receive electromagnetic induction from large transformer, high frequency heater, UPS,
etc.
9) Within 2500 m above sea level(storage within 4600m)
8