Okolab UNO-T User manual

UNO-STAGE-TOP-INCUBATOR
UNO-T
UNO-T-H-PREMIXED
UNO-T-H-CO2
Manual
IST872_REV07
SV 1.6.1.0

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Index
1PREFACE .....................................................................................................................................................................................1
2SYMBOL DESCRIPTION ..........................................................................................................................................................2
SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................2
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................2
3SAFETY NOTES..........................................................................................................................................................................3
4EQUIPMENT OVERVIEW........................................................................................................................................................5
UNO-T..........................................................................................................................................................................................................5
UNO-T-H-PREMIXED ............................................................................................................................................................................5
UNO-T-H-CO2...........................................................................................................................................................................................6
5COMPATIBILITY.......................................................................................................................................................................8
6EQUIPMENT DESCRIPTION ..................................................................................................................................................9
7INSTALLATION ...................................................................................................................................................................... 11
CONNECTION OF THE CHAMBER BASE AND THE CHAMBER LID.......................................................................................................11
CONNECTION OF GF-MIXER-HM (HUMIDITY MODULE)................................................................................................................11
GAS CONNECTION .....................................................................................................................................................................................14
7.3.1 Gas connection for UNO-T-H-PREMIXED .........................................................................................................................14
7.3.2 Gas Connection for UNO-T-H-CO2.......................................................................................................................................16
7.3.2.1 How to use 2GF-MIXER.................................................................................................................................................................................. 18
CONNECTION OF TSENSOR.....................................................................................................................................................................20
CONNECTION OF THE OBJ-COLLAR (OPTIONAL) .............................................................................................................................20
MINI-USB PORT ......................................................................................................................................................................................21
7.6.1 Connection of MINI-USB-OTG cable....................................................................................................................................21
7.6.2 Connection of MINI-USB cable ..............................................................................................................................................21
RS232 SERIAL PORT ...............................................................................................................................................................................22
8USER INTERFACE .................................................................................................................................................................. 24
HOME PAGE................................................................................................................................................................................................24
8.1.1 How to enter the Setpoint .......................................................................................................................................................25
8.1.2 Graph view .....................................................................................................................................................................................25
8.1.3 Controller Status: colours led and meaning ....................................................................................................................26
SETTINGS....................................................................................................................................................................................................27
8.2.1 Control Mode.................................................................................................................................................................................27
8.2.1.1 Chamber Control mode ................................................................................................................................................................................. 28
8.2.1.2 Sample Control mode.....................................................................................................................................................................................29
8.2.2 Chamber & Insert........................................................................................................................................................................31
8.2.2.1 Compatible Okolab H301 incubating chamber List.......................................................................................................................... 33
8.2.3 Objective Heater ..........................................................................................................................................................................35
8.2.4 Humidity .........................................................................................................................................................................................36
8.2.5 Calibrations....................................................................................................................................................................................38
8.2.5.1 Chamber............................................................................................................................................................................................................... 39
8.2.5.2 T Sensor................................................................................................................................................................................................................42
8.2.5.3 Obj. Heater.......................................................................................................................................................................................................... 44
8.2.6Logging.............................................................................................................................................................................................47
8.2.7 Alarms ..............................................................................................................................................................................................51
8.2.8 Display .............................................................................................................................................................................................52
8.2.8.1 Options..................................................................................................................................................................................................................53
8.2.8.2 Brightness ........................................................................................................................................................................................................... 53
8.2.8.3 Calibration..........................................................................................................................................................................................................54
8.2.8.4 Date & Time........................................................................................................................................................................................................ 54
8.2.8.5 Visual Effects...................................................................................................................................................................................................... 55

8.2.8.6 Factory Reset..................................................................................................................................................................................................... 56
8.2.8.7 Password .............................................................................................................................................................................................................56
8.2.8.8 Summary .............................................................................................................................................................................................................57
8.2.8.9 Auto on/off ......................................................................................................................................................................................................... 57
STATUS OVERVIEW...................................................................................................................................................................................59
ICON AND GLANCE MODE VIEW.............................................................................................................................................................59
INFO PAGE ..................................................................................................................................................................................................59
9TOUCH SCREEN CALIBRATION ........................................................................................................................................ 61
10 CLEANING & MAINTENANCE......................................................................................................................................... 62
11 SUPPORT ............................................................................................................................................................................. 63
12 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 64
13 TROUBLESHOOTING........................................................................................................................................................ 66
14 FIGURE LIST........................................................................................................................................................................ 67
15 TABLE LIST ......................................................................................................................................................................... 68
16 MANUAL REVISION TABLE ............................................................................................................................................ 68

1
1Preface
UNO-STAGE-TOP-INCUBATOR ensures the highest temperature accuracy on sample temperature by
controlling simultaneously up to six integrated channels with a state-of-art multichannel controller.
UNO-STAGE-TOP-INCUBATOR is designed to control the environmental conditions inside the top stage
incubator, where specimens are located. UNO-STAGE-TOP-INCUBATOR features a system to control the
temperature from 3°C above ambient temperature (minimum temperature set point 25°C) to 60°C. The 4.3'' touch
screen controller interface allows working in both Chamber and Sample control mode. The temperature accuracy
is ± 0.1°C in Sample Control mode and ± 0.3°C in Chamber Control mode, if Room Temperature is stable within ±
1°C. UNO-STAGE-TOP-INCUBATOR includes an external fine gauge thermocouple for sample feedback operation.
UNO STAGE-TOP-INCUBATOR requires the selection of one stage top chamber. It is compatible with all
Okolab H301 incubating chamber (to be ordered separately).
UNO-STAGE-TOP-INCUBATOR is available in three different configurations:
•UNO-T including:
oUNO-CONTROLLER - the temperature controller.
•UNO-T-H-PREMIXED, stage top incubator's controller for application with premixed gas, including
oUNO-CONTROLLER - the temperature controller.
oGF-REGULATOR - flow rate regulator for the premixed gas. The regulator, equipped with a calibrated
output orifice, allows to set the desired output gas flow rate (in the range 0.1 - 0.4 l/min) by regulating
manually the pressure.
oGF-MIXER-HM - the Humidity Module. Dry premixed gas is humidified by flowing over warm water, heated
up by the Humidifier. The Humidifier is composed of a glass bottle, Humidifier Bottle, and its heater A
heated tube delivers the humid premixed gas to the stage top incubator preventing moisture
condensation.
•UNO-T-H-CO2, stage top incubator's controller for application with 100% CO2 and background air, including:
oUNO-CONTROLLER - the temperature controller.
oGF-MIXER-HM - the Humidity Module.
o2GF-MIXER - a two-channel gas flow regulator. The 2G-MIXER is a manual mixer that allows the mixing of
CO2 and air at the desired concentration by two floating ball flow meters. The CO2 concentration can be
regulated in the range 0-15% in air with an accuracy of ±1%.
oOKO-AP - air pump. It enables to use background air as a convenient alternative to the compressed air.
UNO-STAGE-TOP-INCUBATOR is also compatible with Okolab objective Heater, OBJ-COLLAR series,
recommended for immersion objectives. Collars connect directly to the controller and are calibrated with
automatic routine.
The controller features on-board memory for data logging and mini-USB port for data download.
In addition, data logging is possible via DATA-LOG Okolab software and via following third-party Imaging
software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN Black software is
available upon request.
UNO-STAGE-TOP-INCUBATOR iso also capable to receive TTL input (requires to order separately TTL-
CABLE).

2
2Symbol description
This paragraph describes the symbols used in this manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol warns you to read the user manual before starting the device.

3
3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−The products labels can be found on the bottom panel of the Main Box.
−Do not disassemble any part of the system.
−Do not use a volatile solvent such as paint thinner to clean the instrument, because
deformation or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−Do NOT go in close contact with or breathe any gas stream whose composition is different
from that of ambient air.
−Prevent throttling and kinking of tubing.
−Check tubing time to time for possible material usage.
−Check that all tubing ends are well inserted into the connectors so they cannot slip off
−This device is not designed for use for medical applications.
−Install safety valves and adequate pressure regulators on gas lines before the unit input
connectors.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long
period of time.
−PRESSURIZED GAS. Secure all connections with hose clamps. Never exceed the input
pressure limit of 10 barg (145 psig). Bleed all lines before disconnecting. Wear safety
goggles if needed. If pressure regulators are not within sight and reach, make sure at least
one shut off valve is within reach.
−LOW OXYGEN ATMOSPHERES and HIGH CO2 ATMOSPHERE. Never enter a room or
enclosure which has a low oxygen/high CO2 atmosphere because of severe danger of
suffocation. Only operate in well-ventilated room. A small amount for carbon dioxide gas
leaks continuously out of the instrument and should never be allowed to build up in the
room.
−VENTILATION. Unit should be situated so that its location or position does not interfere
with proper ventilation. Neither the gas mixer nor stream destinations should be in poorly
ventilated areas.
−Unit should be situated away from heat sources such as open flames, radiators, heat
registers, stoves, or other appliances or processes that produce heat.

4
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not disconnect cables while in operation.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Unit should never be used where it can fall or be pushed into water.
International caution symbol marks this device. It is important to read the “Safety
Notes” before installing, using and commissioning this device, as the notes contain
important information relating to safety and EMC. Not following these instructions
can result in damage or breakdown of the device and its accessories
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

5
4Equipment Overview
UNO-T
Figure 1 illustrates the equipment overview for UNO-T configuration:
1. UNO-CONTROLLER (x1), temperature controller
2. Y-CABLE-1 (x1). To connect chamber’s base and lid to the temperature controller.
3. 24V-DC Power Adapter (x1).
4. OBJ-COLLAR (optional)
5. Okolab H301 incubating chamber (required, to be ordered separately).
6. MINI-USB-OTG cable (x1). To connect a USB pen to the temperature controller for data download.
7. Temperature Sensor (T Sensor) and extension (x1).
1
4
3
5
2
6
7
Figure 1. UNO-T Equipment Overview.
UNO-T-H-PREMIXED
Figure 2 illustrates the equipment overview for UNO-T-H-PREMIXED configuration:
1. UNO-CONTROLLER (x1), temperature controller
2. Y-CABLE-2 (x1). To connect the Humidifier and the Heated tube to the temperature controller.
3. Y-CABLE-1 (x1). To connect chamber’s base and lid to the temperature controller.
4. 24V-DC Power Adapter (x1).
5. MINI-USB-OTG cable (x1). To connect a USB pen to the temperature controller for data download.
6. Temperature Sensor (T Sensor) and extension (x1).
7. TUBE-A (x1). Transparent rigid tube 6mm O.D.. Use TUBE-A to connect the premixed gas supply to the gas
input of the Gf-REGULATOR.

6
8. GF-REGULATOR (x1), flow rate regulator for the premixed gas.
9. TUBE-C (x1). Blue rigid tube 6mm O.D. with attached a 4mm I.D. silicon transparent with a PTFE filter in the
middle. Use TUBE C to connect the gas output of the GF-REGULATOR to the gas input connector of Humidifier.
10. Humidifer (x1). It is composed of a glass bottle, Humidifier Bottle, with TUBE E (6 mm O.D. silicone tube with
a glass bubbler at the end) and its heater, Humidifier Heater.
11. Heated Tube (x1). Use Heated Tube to connect the Humidifier to Okolab H301 incubating chamber.
12. Okolab H301 incubating chamber (required, to be ordered separately).
13. OBJ-COLLAR (optional).
1
4
6
2
5
8
9
12
13
3
To premixed
gas supply
11
7
10
Figure 2. UNO-T-H-PREMIXED Equipment Overview.
UNO-T-H-CO2
Figure 3 illustrates the equipment overview for UNO-T-H-CO2 configuration:
1. UNO-CONTROLLER (x1), temperature controller
2. Y-CABLE-2 (x1). To connect Humidifier and the Heated tube to the temperature controller.
3. Y-CABLE-1 (x1). To connect chamber’s base and lid to the temperature controller.
4. 24V-DC Power Adapter (x1).
5. MINI-USB-OTG cable (x1). To connect a USB pen to the temperature controller for data download.
6. Temperature Sensor (T Sensor) and extension (x1).
7. OKO-AP (x1), air pump with 24V-DC power adapter.

7
8. TUBE-B (x1) Transparent silicon 4mm I.D. tube with a PTFE filter in the middle connected at both ends to a
blue rigid tube 6 mm O.D.. Use TUBE-B to connect OKO-AP to the air input port on the rear panel of 2GF-MIXER.
9. 2GF-MIXER (x1), manual Mixer to mix Air and CO2.
10. Pressure Gauge for CO2 (x1). Install pressure gauge between the pure CO2 supply pressure regulator and
the corresponding CO2 input port on the rear panel of 2GF-MIXER.
11. TUBE-A(x1). Transparent rigid tube 6mm O.D.. Use TUBE-A to connect the output port of the pressure gauge
to the CO2 input port on the rear panel of 2GF-MIXER.
12. TUBE-C (x1). Blue rigid tube 6mm O.D. connected to a 4mm I.D. silicon transparent with a PTFE filter in the
middle. Use TUBE C to connect the gas output of the GR-REGULATOR to the gas input connector of Humidifier.
13. Humidifier (x1). It is composed of a glass bottle, Humidifier Bottle, with TUBE E (6 mm OD silicon tube with
a glass bubbler at the end) and its heater, Humidifier Heater.
14. Heated Tube (x1). Use Heated Tube to connect the Humidifier to Okolab H301 incubating chamber.
15. Okolab H301 incubating chamber (required, to be ordered separately).
16. OBJ-COLLAR (optional)
1
4
6
2
5
7
12
16
8
9
13
14
15
3
10
11
To CO2
supply
Figure 3. UNO-T-H-CO2 Equipment Overview.

8
Note ► For U.S. shipment, Okolab provides a push to fit adapter from ¼” to 6 mm rigid tube.
5Compatibility
UNO-STAGE-TOP-INCUBATOR is compatible with:
•Okolab H301 incubating chamber (required, to be ordered separately), with heated lid and base,
chamber riser with one or more plate adapters. Refer to paragraph 8.2.2.1 for the complete list of
compatible H301 incubating chamber.
•OBJ-COLLAR series (optional), recommended for immersion objectives.
•DATA-LOG (optional). Software used to log, view, and store data from a PC. It may be used to change
parameters of the controllers via PC. Data can be exported in .xlsx format.
Figure 4. DATA LOG software.

9
6Equipment Description
Figure 5 illustrates UNO-CONTROLLER left panel:
1. MINI-USB port. To connect the supplied MINI-USB-OTG cable or a standard MINI-USB cable.
2. RS232 Serial port. To connect a standard RS232 Serial cable.
12
Figure 5. UNO-CONTROLLER Left Panel Overview.
Figure 6 illustrated UNO-CONTROLLER rear panel:
3. Power input
4. Base / Lid connector. To provide power to the Chamber Base and Chamber Lid.
5. Humidifier / Heated Tube connector. To provide power to Humidifier and Heated Tube.
6. Obj. Heater connector. To provide power to OBJ-COLLAR.
7. T Sensor connector
345 6 7
Figure 6. UNO-CONTROLLER Rear Panel Overview.
Figure 7 illustrates UNO-CONTROLLER front panel:
8. Status Led.
9. ON/OFF Button.

10
8 9
Figure 7. UNO-CONTROLLER Front Panel Overview.

11
7Installation
The following paragraph illustrates how to install UNO-STAGE-TOP-INCUBATOR.
The following instructions shall give you the possibility to install the unit quickly. For safe operation
of the unit, it is absolutely necessary to read carefully all the instructions and safety notes.
Connection of the Chamber Base and the Chamber Lid
Follow the instructions below to connect the Base and the Lid of the Okolab H301 incubating chamber to
UNO-CONTROLLER (see Figure 8):
1. Connect the Y-CABLE-1 connector to “Base/Lid”connector on the rear panel of UNO-CONTROLLER.
2. Connect the Base cable of the Chamber Base to its corresponding connector of WP-CABLE. Connect the
RED labeled connector of WP-CABLE to its corresponding connector of Y-CABLE-1.
3. Connect the Lid cable of the Chamber Lid to its corresponding connector of WP-CABLE. Connect the
YELLOW labeled connector of WP-CABLE to its corresponding connector of Y-CABLE-1.
Chamber Lid
Chamber Base
UNO-CONTROLLER
Y-CABLE-1
WP-CABLE
WP-CABLE
Figure 8. Chamber Base and Chamber Lid - Connection.
Tip ►Connecting cables are equipped with identification tags to guide you through the installation process.
Note ►If the Base cable or the Lid cable is equipped with a DIN connector, attach it to its corresponding
connector of Y-CABLE-1.
Connection of GF-MIXER-HM (Humidity Module)
This section describes how to install GF-MIXER-HM, the Humidity Module, included in UNO-T-H-
PREMIXED and UNO-T-H-CO2 configurations. Follow the steps below for a correct installation:
1. Connect TUBE-E to the connector located inside the Humidifier Cap (see image 1 in Figure 9).
2. Fill the Humidifier Bottle with distilled water and insert the TUBE-E with the Humidifier Cap. Make sure
to place the O-RING on the lip of Humidifier Bottle’s neck (see image 2 in Figure 9).
3. Screw the Humidifier Cap and place the Humidifier Bottle in its heater (see image 3 in Figure 9).
The Humidifier Cap is equipped with a Gas Inlet connector, barb connector for 4 mm I.D. silicon tube, and
a Gas Outlet connector, push to fit connector for 6 mm O.D. rigid tube (see image 4 in Figure 9).

12
1 2
3
Fill it with
distilled water
O-RING
TUBE-E
Humidifier
Cap
Humidifier
Bottle
Humidifier
Heater
4
Gas Inlet
Connector Gas Outlet
Connector
Figure 9. Humidifier - Assembly.
Connect the rigid end of Heated Tube to the Gas Outlet connector of Humidifier. Connect the silicon end of
Heated Tube to the gas input connector located on a corner of Okolab H301 incubating chamber (see Figure 10).
Tip ►Make sure to push the tubes all the way into the connectors thus avoiding any gas leak.

13
Heated Tube
Humidifier
H301 Chamber
Figure 10. Heated Tube - Assembly.
Follow the instructions below to connect GF-MIXER-HM, the Humidity Module, to UNO-CONTROLLER (see
Figure 11):
1. Connect the Y-CABLE-2 connector to “Humidifier/Heated Tube”connector on the rear panel of UNO-
CONTROLLER.
2. Connect the GREEN labeled Humidifier Heater cable of the Humidifier to its corresponding connector of
Y-CABLE-2.
3. Connect the Heated Tube cable to its corresponding connector of WP-CABLE. Connect WP-CABLE to the
GREY labeled connector of Y-CABLE-2.
Humidifier
Heater
Heated Tube
UNO-CONTROLLER
Y-CABLE-2
WP-CABLE
Figure 11. GF-MIXER-HM - Connection.
Tip ►Connecting cables are equipped with identification tags to guide you through the installation process.

14
Gas Connection
7.3.1 Gas connection for UNO-T-H-PREMIXED
UNO-T-H-PREMIXED is suitable or applications with premixed gas supply. Follow the steps below for a
correct installation:
1. Install the GF-REGULATOR between the premixed gas supply and the Humidifier (see Figure 13). Use
TUBE-A to connect the premixed gas supply to the push to fit input connector for a 6 mm O.D. rigid tube
of the GF-REGULATOR (see Figure 13).
During this operation make sure that both the premixed gas tank and the GF-REGULATOR are closed.
Be careful to follow the direction of the arrow on the rear of the GF-REGULATOR for the correct gas in-out
orientation (see Figure 12).
a. b.
Figure 12. GF-REGULATOR - front and rear view (a - b).
The premixed gas tank must be equipped with a pressure regulator to decrease the pressure from
the pressure tank to 4 barg (58 psig).
Do not exceed 10 barg (145 psig) for GF-REGULATOR inlet gas pressure. This may damage the GF-
REGULATOR.
2. Connect the blue rigid end of TUBE-C to the output connector of GF-REGULATOR. Connect the silicon end
of TUBE-C to the Gas Inlet gas connector of the Humidifier (see Figure 13). TUBE-C is equipped with a
PTFE filter in the middle. Make sure to install the TUBE-C in the direction of the gas flow indicated by the
labels on both ends of TUBE-C and on both sides of the PTFE filter.
Tip ►Replace the PTFE filter each 2-3 months of continuous operation or if you see water in the inner part
of the filter.
Do not use TUBE-C without the PTFE filter.

15
TUBE-C
GF-REGULATOR
FLOW DIRECTION
FLOW DIRECTION
Humidifier
Not Supplied
Premixed Gas
TUBE-A
Figure 13. UNO-T-H-PREMIXED - Gas connection.
Tip ►Make sure to push the tubes all the way into the connectors thus avoiding any gas leak.
The GF-REGULATOR provides an easy output gas flow rate regulation in the range 0.17 –0.41 l/min,
thanks to its calibrated orifice. Regulate the pressure of the GF-REGULATOR to obtain the desired gas flow rate.
Table 1 shows the relation between pressure and the gas flow rate for the GF Regulator.
Pressure
[barg]
Gas Flow Rate
[l/min]
0.2
0.17
0.4
0.22
0.6
0.27
0.8
0.32
1.0
0.37
1.2
0.41
Table 1. GF-REGULATOR- Pressure vs Gas Flow Rate.
Tip ►If the desired gas flow rate is 0.27 l/min, the pressure of the GF-REGULATOR has to be set at 0.6 barg
(8.7 psig).
Tip ►Okolab suggests setting the gas flow rate in the range 0.3 –0.4 l/min. If the total control volume is
Figure 14 shows how to use the GF-REGULATOR correctly:
A. Pull the knob up to release the lock.
B. Rotate the knob until the pressure reaches the desired value (clockwise/counterclockwise to
increase/decrease the pressure).
C. Push the knob down to lock it.

16
AC
B
Figure 14. GF-REGULATION - Use.
7.3.2 Gas Connection for UNO-T-H-CO2
UNO-T-H-CO2 is suitable for applications with 100% CO2 and background air. Follow the steps below for
a correct installation:
1. Install the pressure gauge between the CO2 supply and the 2GF-MIXER (see Figure 15). The pressure
gauge is equipped with a push to fit input connector for a 6 mm O.D. rigid tube.
Tip ►Cut and use TUBE-A according to your needs. TUBE-A is suitable for connecting the CO2 supply to the
gas input of the pressure gauge.
During this operation make sure that both the CO2 tank and the pressure gauge are closed.
Be careful to follow the direction of the arrow on the rear of the pressure gauge for the correct gas in-out
orientation.
The CO2 tank must be equipped with a pressure regulator to decrease the pressure from the pressure
tank to 2 barg (29 psig).
Do not exceed 10 barg (145 psig) for the pressure gauge inlet gas pressure. This may damage the
pressure gauge.
2. Use TUBE-A to connect the output connector of the pressure gauge to the “CO2 In” push to fit connector
on the rear panel of 2GF-MIXER (see Figure 15). Set the pressure at 1 barg (14.5 psig).
3. Connect TUBE-B between the OKO-AP gas connector and the “Air In” push to fit connector on the rear
panel of 2GF-MIXER (see Figure 15). TUBE-B is equipped with a PTFE filter in the middle. Make sure to
install the TUBE-B in the direction of the gas flow indicated by the labels on both ends of TUBE-B and on
both sides of the PTFE filter.