NuAire LabGard NU-545-300E User manual

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LabGard® ES Energy Saver
Class II Laminar Flow
Biosafety Cabinet
Models
NU-545-300E/400E/500E/600E
Bench/Console
Operation and Maintenance Manual
July, 2021
Revision 3
Series C,
Manufactured By:
Nuaire, Inc.
2100 Fernbrook Lane
Plymouth, MN 55447
Toll-Free: 1-800-328-3352
In Minnesota: (763)-553-1270
Fax: [phone]

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Congratulations!
You have just purchased one of the finest Laminar Flow Biosafety Cabinets available. With proper care, maintenance
(certification), and laboratory procedure, this cabinet will give you years of product and personnel protection from particulate
contaminants as prescribed in NSF/ANSI 49. Please read this manual carefully to familiarize yourself with proper installation,
maintenance and operation of the cabinet. Other reference and guideline materials are available through the following web sites;
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.cabs-acsb.ca
www.ebsaweb.eu
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.Nuaire.com

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About this Operation and Maintenance Manual
The information contained in this manual is intended to reflect our current production model along with the more frequently
purchased options. Any unique additions, modifications, or shop drawings are appended in the back flap of this manual, along with
any modifications and/or additions to procedures as outlined in this manual. A copy of the original factory test report is also
appended to this manual. In case this manual and/or test report is lost or misplaced, Nuaire retains a copy in our files. A
replacement copy can be obtained by calling or writing Nuaire, Inc. stating the model number and serial number and a brief
description of the information desired.

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LabGard® ES Energy Saver
Class II Laminar Flow Biosafety Cabinet
Models NU-545-300E/400E/500E/600E Operation and Maintenance Manual
Table of Contents
Section No. 1.....................................................General Information
Section No. 2.....................................................Models and Features
Section No. 3.....................................................Warranty
Section No. 4.....................................................Shipments
Section No. 5.....................................................Installation Instructions
5.1..................................................................... Location
5.2..................................................................... Set-up Instructions
5.3..................................................................... Testing Methods and Equipment
Section No. 6.....................................................Operating the NU-545E
6.1..................................................................... TouchLink™ Control System
6.2..................................................................... Operating Guidelines
6.3..................................................................... Operating Sequence
6.4..................................................................... Ergonomics
6.5..................................................................... Cleaning Procedures
Section No. 7.....................................................General Maintenance
7.1..................................................................... Decontamination
7.2..................................................................... LED Lamp Replacement
7.3..................................................................... HEPA Filter/Motor Replacement
7.4..................................................................... Sliding Window Replacement and Manual Adjustment
7.5..................................................................... Airflow Control System Setup and Calibration
7.6..................................................................... HEPA Filter Leak test
7.7..................................................................... Airflow Smoke Pattern Test
7.8..................................................................... Site Installation Assessment Test
7.9..................................................................... Cleanliness Classification Test for Pharmacy Application
7.10................................................................... Main Control Board Description and Replacement
7.11................................................................... Digital Airflow Sensor Description and Replacement
Section No. 8 ....................................................Error Messages, Troubleshooting and Option-Diagnostics
Section No. 9.....................................................Remote Contacts
Section No. 10...................................................Optional Equipment
10.1................................................................... Ultraviolet Light
Section No. 11...................................................Electrical/Environmental Requirements
Section No. 12...................................................Disposal and Recycle
Manual Drawings
ACD-16296 ......................................NU-545 Airflow Schematic
CD-000047.......................................NU-545-300E Specification Drawing
CD-000048.......................................NU-545-400E Specification Drawing
CD-000049.......................................NU-545-500E Specification Drawing
CD-000050.......................................NU-545-600E Specification Drawing
BCD-16882 ......................................Drain Valve Installation
BCD-16509 ......................................Auto Decontamination Setup
Assembly Drawings
BCD-16633 ......................................Base Stand Assembly
BCD-16301 ......................................Control Center and Front Decorative Panel Assembly
BCD-16297 ......................................Sliding Window Assembly and Adjustment
BCD-16299 ......................................Base Cabinet Assembly
BCD-16298 ......................................NU-545 Window Assembly Hinge Operation
BCD-12281 ......................................Auto Window Switch/Cable Adjustment and Maintenance
BCD-12282 ......................................Auto Window Cable and Window Assembly
Electrical Schematics
CD-000026 (sheet 1 and 2)..............NU-545-300E/400E/500E/600E Electrical Schematic

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LabGard® ES Energy Saver
Class II, Type A2 Laminar Flow
Biosafety Cabinet
Models
NU-545-300E/400E/500E/600E
Manufactured by:
Nuaire, Inc. - Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The LabGard® ES Model NU-545E Laminar Flow Biosafety Cabinet (LFBSC) is a bench/table top model, optionally available
with a base support stand, for operation as a console model. The LabGard® ES model NU-545E utilizes an Energy Saver DC
ECM motor optimally determined forward curved fan for each model size/width to maximize both energy efficiency and
filter loading capacity. The Energy Saver ECM motor is controlled airflow setpoints via a solid-state DC motor controller
with digital dual thermistor airflow sensors that provide an automatic compensation (constant volume control) for both
filter loading and line voltage variances.
The Laminar Flow Biosafety Cabinet, (LFBSC) is a product resulting from the development of the "laminar flow" principle
(see Airflow Schematic) and the application of environmental controls as required in the field of biological research or
chemical containment. The LFBSC, when used with proper technique, is an effective primary engineering control in
obtaining the optimum product quality while reducing the potential for exposure of both product and personnel to airborne
biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product
operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.
The NU-545E bench LFBSC meets the requirements of a Class II, since the cabinet conforms to the following requirements:
1. Maintains a minimum inflow velocity of 100 LFPM (.51mps) through the work access opening.
2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum.
3. Discharges a percentage of air to the outside atmosphere after HEPA filtration.
4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by negative pressure.
If working with volatile chemicals, the cabinet must be connected to an external exhaust system with a canopy transition.
Type A2 cabinets may be used for work with volatile chemicals if permitted by a chemical risk assessment.

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1.2 Safety Instructions
These safety instructions describe the safety features of the LabGard® ES Model NU-545E LFBSC.
The safety cabinet has been manufactured using the latest technological developments and has been thoroughly tested
before delivery. However, the cabinet may present potential hazards if it is not installed and used as instructed for its
intended purpose or is used outside of operating parameters. Therefore, the following procedures must always be
observed:
•The safety cabinet must be operated only by trained and authorized personnel.
•For any operation of this cabinet, the operator must prepare clear and concise written instructions for operating
and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical regulations, in
particular.
owhich decontamination measures are to be applied for the cabinet and accessories
owhich protective measures apply while specific agents are used
owhich measures are to be taken in the case of an accident
•Repairs to the device must be carried out only by trained and authorized expert personnel.
•Keep these operating instructions close to the cabinet so that safety instructions and important information are
always accessible.
•Should you encounter problems that are not detailed adequately in the operating instructions, please contact your
Nuaire Representative of Nuaire technical Services.
1.3 Explanation of Symbols
NOTE:
CAUTION used without the safety alert symbol
indicates a potentially hazardous situation which, if
not avoided, may result in property damage.
Potential electrical hazard, only qualified person to
access.
Used for important information.
Biohazard
Ground, Earth
CAUTION

Chemical Hazard
Flammable Hazard
Hazardous Gases! Personal
Protection Equipment Required.
Lead Free
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, could result in death of
serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor or
moderate injury.
WARNING
!
CAUTION
!

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2.0 Models and Features
The model NU-545E, LabGard® ES, Class II Laminar Flow Biosafety Cabinet is manufactured in four sizes:
3 ft. (.9m)
4 ft. (1.2m)
5 ft. (1.5m)
6 ft. (1.8m)

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