YSI 2700D User manual

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
Biochemistry Analyzer

Who’s
Minding
the Planet?
find out at 
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i

 
1.1 Description 1-1
1.2 Standard Features 1-3
1.3 General Specifications 1-4
1.4 Chemistry Performance Specifications 1-5
1.5 How to Use this Manual 1-8
  
2.1 Unpacking and Notes on Safety 2-1
2.2 Major Component Identification 2-2
2.3 Reagent Preparation 2-5
2.4 Enzyme Membrane Installation 2-6
2.5 Power Up Procedures 2-8
2.6 Fluid System Priming 2-9
2.7 Printer Paper Installation 2-11
2.8 Instrument Parameter Programming 2-12
2.9 Initial Setup Example: Step-by-Step 2-15
2.10 Probe Baseline Check 2-25
  
3.1 Main Menu 3-1
3.2 Run Mode 3-2
3.3 Standby Mode 3-7
3.4 Daily Operational Checks 3-7
  
4.1 Principle 4-1
4.2 Sample Preparation 4-2
4.3 Measurement Parameter Setup Information 4-3
4.4 D-Glucose (Dextrose) 4-5
4.5 L-Lactate 4-6
4.6 Sucrose 4-7
4.7 Lactose 4-8
4.8 Ethanol 4-9
4.9 Starch 4-10
4.10 Choline 4-11
4.11 Galactose 4-12
4.12 Hydrogen Peroxide 4-13
4.13 L-Glutamate (L-Glutamic Acid) 4-14
4.14 L-Glutamine 4-15
4.15 Methanol 4-16
4.16 Simultaneous Dextrose and L-Lactate 4-17
4.17 Simultaneous Dextrose and Sucrose 4-18
4.18 Simultaneous L-Glutamate and L-Glutamine 4-20
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ii
  
5.1 Introduction 5-1
5.2 Service Selections 5-3
5.3 Setup Selections 5-5
5.4 Diagnostic Selections 5-17
   
6.1 Sensor Technology 6-1
6.2 Fluid System 6-3
6.3 Measurement Methodology 6-4
6.4 Baseline Stability 6-4
6.5 Calibration 6-5
6.6 Linearity 6-5
6.7 Temperature Compensation 6-6
6.8 Level Sensing and Sipper Interference 6-6
6.9 Software Structure 6-7
! 
7.1 Daily Maintenance 7-1
7.2 Calibration Pumping System Maintenance 7-3
7.3 Sample Chamber Cleaning 7-4
7.4 Membrane Replacement 7-5
7.5 Probe Cleaning 7-6
7.6 Tubing Replacement 7-7
7.7 Sipper Replacement 7-9
7.8 Sipper Ground Cable 7-9
7.9 Fuse Replacement 7-10
7.10 Sipper Pump Seal Replacement 7-11
7.11 Sipper Mechanism Lubrication 7-13
" #$%
8.1 Printout Information 8-2
8.2 Troubleshooting Chart 8-4
& 
9.1 Communications Protocol 9-1
9.2 Communications Modes 9-5
9.3 Communications Commands 9-6
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iii
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'(  /  I-1
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iv

Figure 2.1 Inside Front View of the 2700 SELECT 2-2
Figure 2.2 Back View of the 2700 SELECT 2-4
Figure 2.3 Bottles and Level Sensor Cables 2-5
Figure 2.4 Sample Chamber/Sensors 2-6
Figure 2.5 Enzyme Membrane Installation 2-7
Figure 2.6 Line Voltage Selector 2-8
Figure 2.7 Sipper Adjustment Position 2-10
Figure 3.1 2700 SELECT Software Structure 3-1
Figure 3.2 Sampling Stations 3-4
Figure 3.3 The Test Tube Holder Pivoted Out 3-4
Figure 3.4 The Manual Station 3-6
Figure 5.1 2700 SELECT Software Structure 5-1
Figure 5.2 YSI 2700 SELECT Menu Flow Chart 5-2
Figure 5.3 2700 SELECT Major Components 5-17
Figure 6.1 Sensor Probe and Enzyme Membrane 6-1
Figure 6.2 2700 SELECT Fluid System 6-3
Figure 6.3 Typical Sensor Response 6-4
Figure 6.4 Aging Membrane Response 6-5
Figure 6.5 2700 SELECT Software Structure 6-7
Figure 7.1 Sample Chamber Illustration 7-5
Figure 7.2 Tubing Connections 7-7
Figure 7.3 Pump Tubing installation 7-8
Figure 7.4 Pump Assembly 7-9
Figure 7.5 Fuse Replacement 7-10
Figure 7.6 Sipper Pump Head Removal 7-11
Figure 7.7 Sipper Pump Seal Replacement 7-12
Figure 7.8 Sipper Pump Plunger Position 7-12
Figure 7.9 Sipper Mechanism Drive Screw and Guide Rods 7-13
Figure 9.1 RS-232 Signal Description and Direction 9-1
Figure 9.2 Full Handshaking DTE Interface 9-2
Figure 9.3 Three Wire DTE Interface 9-2
Figure 9.4 2700 SELECT Data Report Format 9-7
Figure 9.5 Report Format Field Information 9-8
Figure 9.6 RM Command Report Format 9-15
Figure 9.7 RI Command Report Format 9-16
Figure G.1 Line Power Cord and Plug Wiring G-1
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1-1
 
 
TheYSI Model 2700 SELECT Biochemistry Analyzer is a laboratory instrument intended for
use in research, food-processing and bioprocessing applications. THE MODEL 2700 SELECT
IS NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN
PERFORMANCE EVALUATION.
The Model 2700 SELECT offers precision and specificity comparable to more time consuming
and rigorous methods, but with greater speed, convenience and sensitivity. Many of the
conditions which interfere with refractometers, density meters and manual methods are of little
concern when using the Model 2700 SELECT. Measurements are virtually unaffected by
sample color, turbidity, density, viscosity, pH, volatility, specific gravity, temperature, index of
refraction, optical activity, or the presence of proteins or other biochemicals.
The Model 2700 SELECT can be set up to measure a number of different analytes, but
switching between them may entail some time and effort. Configured as a single channel unit,
the Model 2700 SELECT provides quick measurements of any one of the following analytes:
•D-Glucose (Dextrose)
•L-Lactate
•Sucrose
•Lactose
•Ethanol
•L-Glutamate
•Choline
•L-Glutamine
•Methanol
•Galactose*
•Hydrogen Peroxide*
Configured as a dual channel unit, the Model 2700 SELECT provides quick, simultaneous
measurements of combinations of the following analytes:
•Dextrose and L-Lactate
•Dextrose and Sucrose
•L-Glutamate and L-Glutamine
*YSI does not currently offer calibration standards for these analytes.
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1-2
Flexibility is an important feature of the product. Within practical limits a large number of
measurement parameters can be selected to fit your application. In addition, the default
parameters of many system functions can be changed to fit your needs.
Selectable sample size allows you to extend the dynamic range of the analyzer and improves
precision and accuracy. The aspirated sample size can be specified from a minimum of 5
microliters to a maximum of 65 microliters.
The Model 2700 SELECT is equipped with automatic self-calibration. However, within limits,
the frequency of autocalibration can be programmed to meet your needs.
If instrument calibration is desired upon command only, the self-calibration feature can be
disabled and calibration cycles can be initiated manually via the keypad or commanded by the
host computer via the RS-232 interface.
YSI provides a full line of reagents to support the most common chemistries.
YSI offers the Model 2710 Turntable for batch sampling of up to 24 tubes or vials. The 2710
Turntable is controlled by the 2700 SELECT software via a control cable (provided). The
results of batch sampling are displayed and printed by the 2700 SELECT.
YSI offers the Model 2730 Monitoring and Control Accessory for use in applications where
monitoring and control is desired. The Kit contains a peristaltic pump, external sampling
chamber, and tubing for delivering a sample to the 2700 SELECT. Purge time and sampling
interval are selectable, under control of the 2700 SELECT software. Also included in this
accessory are analog outputs representative of analyte concentrations.
The 2700 SELECT has been designed for future additions to allow even greater versatility
related to sample handling and the measurement of additional analytes. Therefore, as
accessories become available or new chemistries are developed you have a basic instrument
upon which to build.
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1-3
 
•Single and dual chemistry configurations.
•Minimal sample preparation.
•Sample aspiration from a variety of tubes.
•Selectable sample aspiration volume (5 to 65 µL).
•Sample results in about one minute.
•Fluid sensing minimizes carry-over.
•Fluid design washes inside and outside of sipper tube.
•Programmable automatic calibration.
•Selectable concentration units, including g/L, mg/L, mmol/L, %(w/v).
•Liquid crystal display and built-in data printer.
•RS-232 serial port.
•Turntable interface circuitry installed.
•User-accessible service and diagnostic menus.
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1-4
 
Sample size: Adjustable from 5 to 65 microliters (aspirated volume)
Response Time: Sample results in 60 seconds,
Complete sample-to-sample cycle in 90 seconds
(May vary with analyte and sample matrix.)
Power requirement: 110-120 VAC or 220-240 VAC
50-60 Hz
50 Watts nominal
Working environment:
Ambient temperature: 15° to 35° Celsius
Relative humidity: 10% to 90% (non-condensing)
Instrument dimensions: 10.0 x 14.0 x 14.0 inches
25.4 x 35.6 x 35.6 centimeters
Instrument weight: 25 pounds
11.4 kilograms
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1-5
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Dextrose (D-Glucose)
YSI Membrane: 2365
Detection Range: 0-9 g/L at 25 µL sample size,
0-25 g/L at 10 µL sample size
Calibration Point: 2.50 g/L
Linearity Check Point: 9.0 g/L
Precision (CV,n=10): 2%
Linearity (0 to Cal Point): ±2%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 21 days
Membrane O-ring Color: Red
Ethanol
YSI Membrane: 2786
Detection Range: 0-3.2 g/L
Calibration Point: 2.00 g/L
Linearity Check Point: 3.20 g/L
Precision (CV,n=10): 2%
Linearity (0 to Cal Point): ±2%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 5 days
Membrane O-ring Color: Green
Potential Substrate Interference: Methanol
L-Glutamate*
YSI Membrane: 2754
Detection Range: 0-10 mmol/L
Calibration Point: 5.00 mmol/L
Linearity Check Point: 10.0 mmol/L
Precision (CV,n=10): 2%
Linearity (0 to Cal Point): ±2%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 7 days
Membrane O-ring Color: Yellow
*User may configure instrument to measure as monosodium glutamate (MSG).
L-Lactate (L-Lactic Acid)
YSI Membrane: 2329
Detection Range: 0-2.67 g/L
Calibration Point: 0.50 g/L
Linearity Check Point: 2.67 g/L
Precision (CV,n=10): 2%
Linearity (0 to Cal Point): ±2%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 14 days
Membrane O-ring Color: Gray
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1-6
Lactose
YSI Membrane: 2702
Detection Range: 0-25 g/L
Calibration Point: 5.00 g/L
Linearity Check Point: 25.0 g/L
Precision (CV,n=10): 2%
Linearity (0 to Cal Point): ±2%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 10 days
Membrane O-ring Color: White
Sucrose
YSI Membrane: 2703
Detection Range: 0-25 g/L
Calibration Point: 5.00 g/L
Linearity Check Point: 25.0 g/L
Precision (CV,n=10): 2%
Linearity (0 to Cal Point): ±2%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 10 days
Membrane O-ring color: Blue
Choline
YSI Membrane: 2771
Detection Range: 0-450 mg/L
Calibration Point: 175 mg/L
Linearity Check Point: 450 mg/L
Precision (CV,n=10): 2%
Linearity (0 to Cal Point): ±2%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 7 days
Membrane O-ring color: Orange
L-Glutamine*
YSI Membrane: 2735
Detection Range: 0-8 mmol/L
Calibration Point: 5.00 mmol/L
Linearity Check Point: 8.00 mmol/L
Precision (CV,n=5 ): 4%
Linearity (0 to Cal Point): ±4%
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 5 days
Membrane O-ring color: Magenta
*Specs shown are for simultaneous measurement of glutamine and glutamate.
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1-7
Methanol
YSI Membrane: 2725
Detection Range: 0-2.50 g/L
Calibration Point: 1.00 g/L
Linearity Check Point: 2.50 g/L
Precision (CV,n=10 ): 2%
Linearity (0 to Cal Point): ±2% or 0.02 g/L, whichever is greater
Linearity (Cal to Range Max): ±5%
Typical Membrane Working Life: 5 days
Membrane O-ring color: Black
Potential Substrate Interference: Ethanol
NOTE: For the following chemistriesYSI believes that you will be able to measure these
analytes for many applications in the ranges specified below. However,YSI makes no claims
with respect to precision or linearity. For each analyte below you will need to prepare the
calibrator and linearity standards for your application.
YSI also recommends that you confirm that no significant levels of interfering substrates are
present in your samples. For example, we know that lactose and galactose in the same sample
may interfere if significant concentrations of both exist. Also dextrose will read at a sucrose
membrane, however, by using the dual channel approach to dextrose and sucrose, this is
automatically accounted for by the software.
Galactose
Membrane: 2702
Detection Range: 0-25 g/L
Typical Membrane Working Life: 10 days
Membrane O-ring Color: White
User Must Provide: Calibrator solution
Potential Substrate Interference: Lactose
Hydrogen Peroxide
Membrane: 2701
Detection Range: 0-600 mg/L
Typical Membrane Working Life: 21 days
Membrane O-ring Color: Yellow
User Must Provide: Calibrator solution
Catalase-free Supply Buffer
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1-8
% &'!(
If your instrument is configured for single channel chemistry, this instruction manual contains
more information than you require, but the measurement technology, and the principles and
techniques of operation remain the same.
This manual is organized in such a way as to give you the quickest possible start in operating
the instrument. However, it cannot be stressed too strongly that informed and safe operation is
more than just a matter of knowing which buttons to push. An understanding of the principles
of operation and potential chemical interferences is necessary for the wisest interpretation of
results. Thorough precautions regarding the handling of biological samples are also essential
for the safety of operators.
The early parts of this manual will teach you how to get the instrument running. Additional
topics are included to help you understand the science it employs, how to use it most effectively
and safely, and how to keep it operating correctly.
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2-1
 
We recommend that your dealer representative orYSI regional representative assist with initial
setup and orientation.YSI warranty and product performance claims, however, are not
dependent upon installation by factory or dealer personnel.
 
Remove the instrument from the shipping container. Be careful not to discard any parts or
supplies. Check off all items on the packing list and inspect all assemblies and components for
damage. In the event of damaged or missing parts, contactYSI Customer Service or your
Dealer Representative immediately.
Note that reagents for the 2700 SELECT Analyzer are not packaged in the same carton as the
instrument. These materials must be ordered separately as starter supplies and will arrive in a
separate package.
DO NOT PLUG THE INSTRUMENT INAT THIS TIME.You should apply power only
when directed to do so in the setup instructions.
If you ordered the 2700 SELECT with the 2710 Turntable, first set up the 2700 SELECT. Once
the 2700 SELECT is operating properly, refer to the YSI 2710 Turntable Operations Manual.
Notes On Safety (Electrical precautions)
1. BEFORE connecting the power cord, check the line voltage selector and confirm that the
selected voltage matches the local power supply (Section 2.5).
2. Use ONLY the line power cord supplied with the instrument. Connect the plug to a
matching three-pronged wall receptacle.
3. Use ONLY fuses of the type supplied (Section 7.9). Replacement power cords and fuses
can be obtained fromYSI, or your Dealer Representative (Appendix F, G).
4. Do NOT use an extension cord without protective grounding.
5. Do NOT remove rear cover. There are no user serviceable parts inside.
6. Repairs are to be performed only by trained and approved personnel.
7. This instrument must be connected to a protectively grounded (earthed) outlet.
8. The following notices are provided in compliance with IEC1010 Part 1 1990.
8.1 Fuses F1-F7 on the main circuit board are type: Subminiature (F) to UL 198G Standard.
Rating: F1-F5, 1A; F6-F7, 2A. These fuses are NOT operator replaceable.
8.2 See Appendix G for mains plug wiring and fusing instructions.
9. If the equipment is used in a manner not specified by YSI, the protection provided by the
equipment may be impaired.
WARNING: For auxiliary connection, refer to the YSI 2710 Turntable Operation Manual or the
YSI 2730 Monitor and Control Accessory User’s Manual. Use with theYSI 2710
Turntable orYSI 2730 Monitor/Control Accessory only.
WARNING: For remote connection, refer to section 9. Communications. Equipment should be
EN 61010 or EN 60950 approved only.
10. The mains (power) switch is for functional purposes ONLY. To disconnect the instrument
from the mains supply, unplug the mains power cord from the back of the instrument.
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2-2
 
Referring to Figure 2.1 and 2.2, read through the following descriptions and familiarize
yourself with the major components.
NOTE: In maintenance kits, service manuals and part lists the "Sipper" may be referred to as
the Sipper Needle or Sipper Tube.
Figure 2.1
Inside Front View of the 2700 SELECT
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2-3
The Buffer Pump draws buffer from its bottle, pumps it through the Sipper Pump body and the
Sipper, and flushes the Sample Chamber.
The Calibrator Pump draws the appropriate standard solution from the Calibrator Bottle and
fills the Calibrator Well in the Sample Chamber.
The Sipper Pump retracts its piston to draw in standard from the Calibrator Well or sample
from a tube or container. It extends its piston to dispense standard or sample into the Sample
Chamber.
The Sipper Arm and Sipper Assembly is raised or lowered by one motor, and moved
horizontally to its various positions by another motor. The positions are: Calibrator Well
(Station #1), Sample Chamber ("home"), Test Tube Holder Station (Station #2), Manual Station
(Station #3), Turntable Station (Station #4) and Monitor Station (Station #5). The Sipper
capacitively senses fluid to control immersion depth and detect errors.
The Stir Bar is a plastic encapsulated magnet. It is activated by a motor housed below the
Sample Chamber. It provides thorough mixing inside the chamber.
The Buffer, Waste and Calibrator Bottles are visible through the front door window for easy
monitoring of fluid levels. A stainless steel shaft projecting into each bottle terminates with a
connector on the lid, providing a signal used to halt operations when the Buffer or Calibrator
Bottles are empty, or when the Waste bottle is full.
The Sample Chamber is made of clear acrylic plastic. White and black holders for the sensor
probes are screwed to either side. The immobilized enzyme membranes on the sensor probes
are mounted on O-rings which act as fluid seals on each side of the Sample Chamber. A
reference or auxiliary electrode is housed in the temperature probe and positioned at the back of
the Sample Chamber. It is held in place by a retainer that threads directly into the Sample
Block. A small black O-ring slips over the temperature probe/electrode to provide the seal. The
Calibrator Well is located behind and to the right of the Sample Chamber entry port.
The Test Tube Holder pivots out to allow insertion or removal of several common size test
tubes.
The Display is a 2 line by 40 character liquid crystal display.
The Printer provides a hard copy record of sample results, calibration currents and errors. It
uses 2 1/4 inch (56 mm) wide thermal paper.
The Keypad is a 20 key membrane switch. It has 0-9 numeric keys, SPACE and BACK keys
and 8 function keys.
The Reset Switch is located on the back of the instrument. It is used to hard reset the operating
system.
The Remote Communication Port is an RS-232 serial port. It is used to interface with host
computers or other laboratory instruments.
The Auxiliary Port is an interface connector for use with accessories like theYSI 2710
Turntable.
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2-4
Figure 2.2
Back View of the 2700 SELECT
The Line Voltage Selector selects either 110-120 or 220-240 volt operation. It houses the fuses
and pulls out of the case for fuse replacement.
The Power Receptacle is a power inlet. One end of the power cord plugs into this receptacle,
while the other end plugs into an electrical outlet.
The Power Switch is an on/off toggle switch (0-off and I-on). It is located on the back of the
instrument.
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2-5
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Prepare the supply buffer and fill the buffer bottle. Powdered buffer concentrate is included in
the starter supplies.YSI 2357 is recommended for use with allYSI Enzyme Membranes except
YSI 2702 Galactose Oxidase membranes (useYSI 2705),YSI 2786 Ethanol Membranes (use
YSI 2787) andYSI 2725 Methanol Membranes (useYSI 1579). AllYSI buffers may be ordered
separately.
»Place about 500 mL of reagent water (distilled and/or deionized) into a one liter flask,
or other clean container. Add two packages ofYSI 2357 Buffer Concentrate and stir.
NOTE:YSI 1579 Carbonate Buffer Concentrate (used with 2725 membranes) must be
reconstituted as instructed on the bottle.
»Add more reagent water until the total volume of solution is between 900 and
1000 mL.
»Stir as necessary, until the buffer chemicals have completely dissolved.
»Disconnect the electrical lead from the level sensor and remove the bottle lid.
IMPORTANT: When adding fresh buffer to the Buffer Supply Bottle or when installing a new
bottle of Calibrator Solution, make every effort to avoid contamination of the lid and level
sensor assemblies.
»Pour the prepared buffer into the supply bottle, replace the lid, and reconnect the lead.
Install the calibrator solution. One or more of the followingYSI calibration standards will be
provided in your starter supplies,YSI 2776 (Dextrose and L-Lactate),YSI 2780 (Sucrose),YSI
2783 (Lactose),YSI 2772 (Choline),YSI 2755 (L-Glutamate),YSI 2736* (L-Glutamine).YSI
calibrators may be purchased separately.
»Unplug the electrical lead from the level sensor in the lid of the empty calibrator bottle
and remove the lid.
»Mark the date of installation on the new bottle ofYSI calibrator solution.
»Screw the lid and level sensor assembly onto the new bottle and place it in the
instrument compartment. Reconnect the electrical lead.
* TheYSI 2736 Glutamine Calibration Standard must be reconstituted as instructed on the
bottle. It is recommended that a sample be poured into a test tube and installed at station #2 for
calibration. Refrigerate the bulk solution when not in use.
IMPORTANT: The level sensor cables should not touch the instrument housing. It is best to
keep the cable connector on the bottle lid pointed toward the front. False messages concerning
fluid levels may result if the bottles are not installed inside the fluid compartment and the
cables routed as shown in Figure 2.3.
Figure 2.3
Bottles and Level Sensor Cables
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2-6
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Each active probe installed in your instrument is fitted with a protective "shipping membrane"
which must be removed and replaced with a new membrane from the starter supplies.
The Sample Chamber is color coded to assist you in membrane installation and setup. The left
side of the chamber has a black probe housing, and the right side has a white probe housing.
(Throughout this manual, whenever we refer to the "black" or "white" probe, the reference is to
the "black" or the "white" side of the sample chamber.)
The 2700 SELECT is shipped to you already configured as a Single Channel or a Dual Channel
unit. For the single channel configuration, the white probe is a blank (see Figure 2.4).
Figure 2.4
Sample Chamber/Sensor Configurations
One or more packs ofYSI Immobilized Enzyme Membranes is provided in the starter supplies.
Each pack contains four membranes. Enzyme Membrane O-rings are color-coded for each type
of chemistry. For dual channel configurations it is important that you note which probe, black
or white, you use to install specific membranes. It will be necessary to assign chemistry to
probe during instrument parameter programming.
For single channel configuration theYSI Enzyme Membrane must be installed on the black
probe. The blank is sealed in the chamber. You need not remove it.
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