YSI Pro 1020 Manual

IOP 019B.0
Effective Date: 1/26/2017
Page 1 of 8
U.S. Fish and Wildlife Service
Marquette Biological Station
3090 Wright Street
Marquette, Michigan 49855
U.S.A.
and
U.S. Fish and Wildlife Service
Ludington Biological Station
5050 Commerce Drive
Ludington, Michigan 49431
U.S.A.
and
Department of Fisheries and Oceans
Sea Lamprey Control Centre
1219 Queen Street East
Sault Ste. Marie, Ontario P6A 2E5
Canada
INSTRUMENT OPERATING PROCEDURE
INSTRUMENT:
Multiparameter Water Quality Instrument
MODEL:
-Pro 1020 Meter (6051020)
-Cable (6051020)
-Probes: pH (605101), DO (605203)
MANUFACTURER:
Yellow Springs Instrument Co., Inc. (YSI)
PRECAUTIONS:
POTENTIAL INTERFERENCES
General –
-Greatest accuracy is achieved if the actual samples to be measured are within 10 ˚C of the
calibration solutions.
-Longer cable lengths introduce more error into the readings.
Dissolved oxygen –
-Chlorine, sulfur dioxide, nitric oxide, and nitrous oxide can affect readings by behaving like
oxygen at the membrane.
-Static electricity may affect readings.
-Gold Cathode - The gold cathode on the sensor must be textured properly. It may become
tarnished or plated with silver after extended periods of use.
-Bubbles under the membrane cap will affect readings.
-Excessive water in calibration/storage sleeve will affect calibration.

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pH –
-Do not measure pH in metal containers.
-Water currents in streams can cause unstable readings.
-pH measurements will drift if the sample is not stirred.
-Do not expose the meter or probe to freezing temperatures.
-High humidity and cold temperatures may affect meter operation.
-Be sure that the temperature sensor is immersed in the sample.
-Buffers degrade from exposure to light - change daily
SAFETY
No special precautions
PROCEDURES:
I. Configuration – The YSI Pro 1020 instrument is capable of simultaneous measurement of multiple
water chemistry parameters including temperature, pH, and dissolved oxygen.
A. Instrument overview
1. Meter – The YSI Pro 1020 handheld meter includes internal hardware and
software for calculation of parameters, a display screen, and a keypad for data
entry and screen navigation. The meter includes an internal barometer.
2. Cable – The instrument cable connects the meter to the sensors. The SLCP uses
the 6051020 cable with an integrated temperature sensor and two sensor ports.
3. Sensors – two sensors are required to measure all of the water chemistry
parameters indicated above. Sensors are installed in the cable bulkhead ports
indicated below to ensure proper function. If measurement of one of the
parameters is not required, the corresponding sensor(s) may be removed and
replaced with a sensor port plug.
a. temperature sensor is permanently incorporated into the cable end
b. pH sensor (605101) installed in port 1
c. polarographic dissolved oxygen sensor (605203) installed in port 2
II. Pro 1020 Key Pad Functions
Key Description
☼
Power and Backlight - Press once to turn instrument on. Press a second
time to turn backlight on. Press a third time to turn backlight off. Press and
hold for 3 seconds to turn instrument off.
Down Arrow - Use to navigate through menus, to navigate through box
options at the bottom of the Run screen and to decrease numerical inputs.
ENTER
Enter - Press to confirm selections, including alpha/numeric key
selections.

Up Arrow - Use to navigate through menus, to navigate through box
options along the bottom of the Run screen and to increase numerical
inputs.
Cal Calibrate - Press and hold for 3 seconds to calibrate. Opens Calibrate
menu from the Run screen.

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Menu Menu - Press to enter the System Setup menu from the Run screen.
III. Meter preparation and maintenance
A. Battery installation and replacement
1. Use a screwdriver to remove the 4 screws securing the battery chamber cover.
2. Lift off the battery chamber cover.
3. Install two alkaline C-size batteries according to the inscribed battery symbols
on the inside of the battery chamber. Make sure batteries are in correct polarity.
4. Close the battery chamber cover and secure with the 4 screws.
5. Press and release the Power button ( ) on the keypad. The LCD will come on;
if not, consult the trouble shooting guide in the Pro 1020 User Manual.
6. Under normal conditions, the average battery life is 425 hours at room
temperature without using the back light. A battery symbol will blink in the
lower, left corner of the display to indicate low batteries when approximately 1
hour of battery life remains.
B. Instrument software setup
1. Press the Power key to turn the instrument on. The instrument will briefly
display the splash screen with the YSI logo then go directly to the main run
screen.
2. Setup Sensors
a. Press Menu button.
b. Dissolved oxygen sensor:
1) Highlight DO Sensor Type, Press enter.
2) Highlight Polaro(black) and press enter to confirm.
3) Highlight ESC exit, press enter
4) Highlight DO Membrane type, press enter.
5) Highlight 1.25 PE Yellow and press enter to confirm.
6) Highlight ESC exit, press enter to return to previous menu
7) Be sure that Local DO and LDS are not enabled ().
c. pH sensor
1) Highlight ISE Sensor Type, Press enter.
2) Highlight pH and select it ().
3) Highlight ESC exit, press enter
4) Highlight ISE Units,press enter
5) Highlight pH and select it ().
6) Highlight ESC exit, press enter to return to previous menu
C. Sensor Installation (This section can be skipped if not installing or replacing any sensors.
1. Remove/unscrew the metal guard or calibration cup if installed.
2. Remove the red rubber port caps if the instrument was being stored without
sensors installed. Ensure that both the sensor connector and sensor port on the
cable are clean and dry and stay that way during this entire procedure.

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3. Install the 1001 pH sensor in port 1 as follows:
a. If an old sensor is installed, remove it by twisting counter-clockwise.
b. Apply a dab of grease to the new sensor o-rings.
c. Unscrew the cap from the sensor storage bottle to relieve tension on the
o-ring and then remove the bottle. Replace the cap on the storage
bottle with the full cap that came with the new sensor to prevent
spilling the storage solution. Pat the sensor dry with a kim wipe.
d. Push the sensor into the connector on the cable until it is properly
seated and only one o-ring is visible. Failure to properly seat the probe
may result in damage.
e. Twist the sensor clockwise to engage threads and finger tighten. Do not
use a tool. This connection is waterproof. Refer to the sensor
installation sheet included with each sensor for detailed instructions.
4. Install the 2003 DO sensor in the DO port as follows:
a. If an old sensor is installed, remove it by twisting counter-clockwise.
b. Apply a dab of grease to the new sensor o-rings.
c. Grasp the sensor with one hand and the sensor connection end of the
cable (bulkhead) in the other.
d. Push the sensor into the connector on the cable until it is properly
seated and only one o-ring is visible. Failure to properly seat the probe
may result in damage.
e. Twist the sensor clockwise to engage threads and finger tighten. Do not
use a tool. This connection is waterproof. Refer to the sensor
installation sheet included with each sensor for detailed instructions.
5. Install the 2003 DO sensor membrane as follows:
a. Change membrane and oxygen probe electrolyte solution every 30
days.
b. Remove the metal sensor guard.
3. If installing a membrane for the first time, remove the protective cap or
remove the old membrane cap by unscrewing it with the probe upside
down.
c. Examine the gold cathode at the tip of the sensor and silver anode along
the shaft of the sensor. If either the silver anode is black in color or the
gold cathode is dull (tarnished), the sensor needs maintenance or
replacement. Refer to the Pro20 Instruction Manual, GENERAL
MAINTENANCE, of polarographic sensor (Model # 605203).
d. Rinse the sensor with de-ionized water.
e. Fill a new membrane cap (5908 Kit - Yellow, 1.25 mil polyethylene)
with oxygen probe electrolyte Solution. Do not touch the membrane
surface.
f. Tap the side of the membrane cap to release air bubbles.
g. Thread the membrane cap onto the sensor with the probe upside down.
h. Replace the metal sensor guard.
IV. Calibration
A. Temperature – All Pro10 cables have built-in temperature sensors. Temperature
calibration is not required nor is it available.
B. pH - The Pro10 pH sensors can be calibrated by performing a 1, 2 or 3-point calibration.
A minimum of 2 point calibration is required for quality assurance purposes in the SLCP.

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At least one of the calibration points must be done with pH 7 buffer. For auto buffer
recognition to work properly with an older or dirty sensor, calibrate in pH 7 buffer first.
For highest accuracy, use fresh, traceable pH buffers and ensure the sensor and
calibration cup are clean. Most applications in the SLCP use a two point calibration:
1. Place the sensor in pH 7 buffer and allow the temperature and pH readings to
stabilize for at least 5 minutes.
2. Press and hold Cal for three seconds.
3. Highlight pH and press enter. If pH is not listed as an option, check the System
Setup menu to ensure pH is enabled in the ISE Sensor Type menu.
4. Highlight 2 point and press enter.
5. If necessary, use the up and down arrow keys to adjust the pH buffer value. Note
the pH mV reading which ideally should be between -50 and +50 in pH 7 buffer.
If the pH mV reading is outside of this range, clean the sensor as described in
the maintenance section of this protocol.
6. Press enter to continue to second point.
7. Rinse the sensor and place it in the second pH buffer (4 for alkalinity
measurements or 10 for stream pH measurements).
8. If necessary, use the up and down arrow keys to adjust the pH buffer value.
9. Wait at least 5 minutes for the pH sensor to stabilize and for the temperature
reading to stabilize. Note the pH mV reading. pH mVs in buffer 4 should be
+159 to 180 mV from the previous buffer 7 pH mV value. pH mVs in buffer 10
should be -159 to 180 mV from the previous buffer 7 pH mV value. If the mV
readings are outside of these ranges, clean the sensor as described in the
maintenance section of this protocol.
10. Press enter to complete the calibration or press Cal to cancel.
11. ‘Calibration Successful’ will display for a few seconds to indicate a successful
calibration and then the instrument will return to the Run screen.
12. If the calibration is unsuccessful, an error message will displayon the screen.
Press the Cal key to exit the calibration error message and return to the Run
screen. See the Troubleshooting section of this manual for possible solutions.
C. DO sensor calibration
1. The supplied sensor storage container (a clear sleeve) can be used for DO
calibration purposes. Moisten the sponge in the storage sleeve or plastic cup
with a small amount of clean water. The sponge should be clean since bacterial
growth may consume oxygen and interfere with the calibration. If using the cup
and you no longer have the sponge, place a small amount of clean water (1/8
inch) in the sleeve instead. Make sure there are no water droplets on the DO
membrane or temperature sensor. Then install the storage sleeve over the
sensors, but do not screw on completely. Leaving 2-3 threads disengaged
ensures venting to the atmosphere. Make sure that DO and temperature sensors
are not immersed in water.
2. Turn the instrument on and wait 15 minutes for the storage container to become

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completely saturated and to allow the sensors to stabilize.
3. Press and hold Cal for 3 seconds. Highlight Dissolved Oxygen and press enter
to confirm.
4. The meter will indicate Calibrating %DO on the display
5. The instrument will use the internal barometer during calibration and will
display this value in the bar at the bottom of the display.
6. Calibration Successful will display for a few seconds to indicate a successful
calibration and then the instrument will return to the Run screen.
7. If the Calibration is unsuccessful, an error message will display on the screen.
Press the Cal key to exit the error message and to return to the Run screen. See
Troubleshooting section of the user manual for possible solutions.
VII. Measurements
A. Ensure the cable and sensors are tightly connected to the meter.
B. Be sure the meter has been calibrated.
C. Place the sensors in the sample to be measured.
D. Shake the probe in the sample to release air bubbles.
E. Allow temperature reading to stabilize.
F. Stir the sample water with the probe at a moderate pace. You must provide 6 inches of
water movement per second across the DO sensor membrane to obtain an accurate
reading. Be careful not to create a vortex which allows air bubbles to pass against the DO
membrane.
G. After at least 5 minutes and once the values stabilize, record the measurement.
IIX. Sensor storage
A. Short term storage
The cable assembly is supplied with a sensor storage container, or sleeve, that attaches to
the cable. The container is used for short-term storage (less than 7 days). Be sure to keep
a small amount of moisture (tap water) in the container during storage. This is done to
maintain a 100% saturated air environment which is ideal for short-term sensor storage.
The sensors should not be submersed in water. The intent is to create a humid air storage
environment.
B. Long term storage
1. Dissolved oxygen sensor - Dissolved oxygen sensors should be stored in a dry
state for long term storage. First, remove the membrane cap and thoroughly
rinse the sensor with clean water. Next, either blow it dry with compressed air or
allow to air dry completely. Install a clean, dry new membrane cap over the
sensor to keep it dry and to protect the electrodes. After storing the sensor for a
long period of time, it is necessary to “condition” the sensor by putting a new
membrane with electrolyte solution on the sensor and then turning the
instrument on to allow the sensor sufficient time to stabilize.

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2. pH sensor - The key to pH sensor storage, short or long-term, is to make certain
that the sensor does not dry out. Sensors which have been allowed to dry out due
to improper storage procedures may be irreparably damaged by the dehydration
and will require replacement. You can try to rehydrate the sensor by soaking it
(preferably overnight) in a potassium chloride solution or a pH 4 buffer before
attempting to calibrate. To store the sensor, remove it from the cable and seal the
vacant port with a port plug. Fill the original shipping/storage vessel (plastic
boot or bottle) with buffer 4 solution and then submerge the sensor into the
solution. The sensor should remain submerged in the solution during the storage
period; therefore, make certain that the vessel is sealed to prevent evaporation
and periodically check the vessel to ensure the sensor does not dry out.
MAINTENANCE:
I. Routine cleaning of pH sensor at the end of each treatment session or when pH response is slow
A. Use clean water and a soft clean cloth, lens cleaning tissue, or cotton swab to remove all
foreign material from the glass bulb or platinum button. Then use a moistened cotton
swab to carefully remove any material that may be blocking the reference electrode
junction of the sensor.
B. Soak the sensor for 10-15 minutes in clean water (Not DI water. Soaking in DI water will
shorten the life span of the probe) containing a few drops of commercial dish washing
liquid. Gently clean the glass bulb and platinum button by rubbing with a cotton swab
soaked in the cleaning solution. Rinse the sensor in clean water, wipe with a cotton swab
saturated with clean water, and then re-rinse with clean water.
II. Additional cleaning when good pH response is not restored using Step I
A. Soak the sensor for 30-60 minutes in one molar hydrochloric acid. Rinse the sensor in clean
water, wipe with a cotton swab saturated with clean water, and then re-rinse with clean water.
To be certain all traces of acid are removed, soak the sensor in clean water (Not DI water.
Soaking in DI water will shorten the life span of the probe) for about an hour with occasional
stirring.
B. If biological contamination of the reference junction is suspected or good response is not
restored, soak the sensor for approximately 1 hour in a 1:1 dilution of household chlorine
bleach. Rinse the sensor with clean water and then soak for at least 1 hour (more if
possible) in clean water (Not DI water. Soaking in DI water will shorten the life span of
the probe) with occasional stirring to remove residual bleach from the junction. Then re-
rinse the sensor with clean water and retest.
III. For additional troubleshooting, see user manual.
REFERENCE:
User Manual for YSI Pro 1020
Quick Start Guide for YSI Pro 1020
Calibration Tips for YSI Professional Plus Multiparameter Water Quality Meter

Jessica M. Barber
Digitally signed by JESSICA
BARBER
Date: 2020.03.10 11:25:00
-04'00'