YSI 2900 series User manual

YSI 2900 Series
Biochemistry Analyzers
Operations and Maintenance Manual

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Table of Contents
1. Introduction ....................................................................................................................................5
Description.................................................................................................................................51.1
User Features............................................................................................................................51.2
Models .......................................................................................................................................51.3
2900 Series General Specifications ..........................................................................................61.4
2960 Online Monitor Specifications...........................................................................................61.5 1.5.1 Monitor ................................................................................................................................6
1.5.2 Analog/Control ....................................................................................................................7
2. Safety...............................................................................................................................................7
Important Safety Instructions.....................................................................................................72.1
Explanation of Symbols.............................................................................................................82.2
3. Getting Started ...............................................................................................................................9
Unpacking..................................................................................................................................93.1
Warranty Card .........................................................................................................................103.2
What You Need .......................................................................................................................103.3
Major Components ..................................................................................................................103.4
4. Basic Setup...................................................................................................................................12
Install Bottle Racks..................................................................................................................134.1 4.1.1 Right Side..........................................................................................................................13
4.1.2 Left Side (2950 models only) ............................................................................................14
Connect Printer........................................................................................................................154.2
Connect AC Power..................................................................................................................154.3
Connect 2960 Online Monitor..................................................................................................164.4
Align Sipper .............................................................................................................................164.5
Configure Instrument Chemistries...........................................................................................194.6 4.6.1 Assign Chemistries to Probes...........................................................................................19
4.6.2 Assign Reagents...............................................................................................................22
Prepare and Install Buffer Solutions........................................................................................244.7 4.7.1 Prepare Buffer...................................................................................................................25
4.7.2 Install Buffer Solution(s)....................................................................................................25
Install Calibrator Solution(s) ....................................................................................................254.8
Install Membranes and ISEs....................................................................................................264.9 4.9.1 Enzyme Membranes.........................................................................................................26
4.9.2 Ion Selective Electrodes....................................................................................................27
Prime the Fluid System ...........................................................................................................294.10
Check Probe Currents.............................................................................................................304.114.11.1 Biosensor Probes .......................................................................................................30
4.11.2 ISE Probes..................................................................................................................30
Enable 21 CFR Part 11 Mode .................................................................................................304.12
5. Running the Instrument...............................................................................................................30
Perform Daily Operational Checks..........................................................................................305.1 5.1.1 Enzyme Membrane Integrity Test.....................................................................................31
5.1.2 Linearity Test.....................................................................................................................33
5.1.3 Results ..............................................................................................................................33
Sample Preparation.................................................................................................................355.2
Run Batch................................................................................................................................355.3

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5.3.1 Create Batches .................................................................................................................35
5.3.2 Load Samples ...................................................................................................................37
5.3.3 Start...................................................................................................................................39
5.3.4 Status................................................................................................................................39
Run Stat...................................................................................................................................395.4
Results.....................................................................................................................................425.5
6. Online Monitor and Control.........................................................................................................42
2960 Installation.......................................................................................................................436.1
Align Sipper .............................................................................................................................466.2
Sample Interface......................................................................................................................466.3 6.3.1 Sterilization........................................................................................................................47
Electrical Interface...................................................................................................................476.4 6.4.1 Analog Outputs .................................................................................................................47
6.4.2 Pump Control Outputs.......................................................................................................48
6.4.3 Auxiliary Connector / Signal List .......................................................................................48
Monitor Setup ..........................................................................................................................486.5 6.5.1 Name.................................................................................................................................49
6.5.2 Flow Rate and Purge Time ...............................................................................................50
6.5.3 Antiseptic...........................................................................................................................50
6.5.4 Filtrate Pump.....................................................................................................................51
6.5.5 Module...............................................................................................................................51
Start Monitor............................................................................................................................566.6 6.6.1 Manual Sample during Monitor Session...........................................................................57
Stop Monitor ............................................................................................................................586.7
Control Setup...........................................................................................................................586.8 6.8.1 Control Type......................................................................................................................58
Print Monitor Configuration......................................................................................................676.9
7. System Configuration..................................................................................................................68
Settings....................................................................................................................................687.1 7.1.1 System ..............................................................................................................................68
7.1.2 Fluid Detection (Bottles)....................................................................................................70
7.1.3 Display...............................................................................................................................71
7.1.4 Auto-Cal Settings ..............................................................................................................73
7.1.5 Scheduler..........................................................................................................................74
7.1.6 21 CFR Part 11 .................................................................................................................76
7.1.7 Date/Time..........................................................................................................................81
Service.....................................................................................................................................827.2 7.2.1 Sipper................................................................................................................................82
7.2.2 Pumps...............................................................................................................................85
7.2.3 Modules.............................................................................................................................86
7.2.4 Stirbar................................................................................................................................87
7.2.5 Monitor ..............................................................................................................................89
Data .........................................................................................................................................907.3 7.3.1 Plate..................................................................................................................................90
7.3.2 Monitor ..............................................................................................................................92
7.3.3 Calibration.........................................................................................................................94
Help .........................................................................................................................................957.4 7.4.1 About.................................................................................................................................95
7.4.2 Software............................................................................................................................96
7.4.3 FAQ...................................................................................................................................97
7.4.4 Training .............................................................................................................................97

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Ethernet Port............................................................................................................................987.5 7.5.1 LAN (Shared Network Connection)...................................................................................98
7.5.2 Router (Private Network Connection) ...............................................................................98
7.5.3 Accessing Stored Data......................................................................................................99
8. Chemistry Setup.........................................................................................................................101
Sample Volume .....................................................................................................................1018.1
Measurement Parameter Information....................................................................................1018.2 8.2.1 Choline............................................................................................................................102
8.2.2 Ethanol/Ethanol-HC ........................................................................................................103
8.2.3 Galactose........................................................................................................................104
8.2.4 D-Glucose (Dextrose) .....................................................................................................105
8.2.5 L-Glutamate (L-Glutamic Acid) .......................................................................................106
8.2.6 L-Glutamine.....................................................................................................................107
8.2.7 Glycerol...........................................................................................................................108
8.2.8 Hydrogen Peroxide .........................................................................................................109
8.2.9 L-Lactate .........................................................................................................................110
8.2.10 Lactose.....................................................................................................................111
8.2.11 Methanol...................................................................................................................112
8.2.12 Sucrose.....................................................................................................................113
8.2.13 Xylose.......................................................................................................................114
8.2.14 Simultaneous Ammonium and Potassium................................................................115
8.2.15 Simultaneous Glucose and L-Lactate ......................................................................116
8.2.16 Simultaneous Glucose and Sucrose ........................................................................117
8.2.17 Simultaneous Glucose and Xylose...........................................................................118
8.2.18 Simultaneous L-Glutamate and L-Glutamine...........................................................119
9. Operational Checks and Maintenance.....................................................................................119
Cleaning, Disinfecting, and Decontaminating........................................................................1199.1 9.1.1 Touch Panel....................................................................................................................119
9.1.2 Decontamination Procedures..........................................................................................119
Daily Maintenance.................................................................................................................1209.2 9.2.1 Empty the Waste Bottle(s) ..............................................................................................120
9.2.2 Check the Calibrator Bottle(s).........................................................................................120
9.2.3 Check the Buffer Bottle(s)...............................................................................................120
9.2.4 Check for Leaks..............................................................................................................120
9.2.5 Clean up Spills ................................................................................................................120
9.2.6 Daily Operational Checks................................................................................................120
Monthly Maintenance ............................................................................................................1209.3 9.3.1 Calibration Pumping System Maintenance.....................................................................120
Preventive Maintenance – 6 months or 1000 Hours.............................................................1219.4 9.4.1 Sample Module Cleaning................................................................................................121
9.4.2 Waste Module Cleaning..................................................................................................122
9.4.3 Enzyme Probe Cleaning .................................................................................................122
9.4.4 ISE Cleaning ...................................................................................................................123
9.4.5 Sipper pump Seal Replacement.....................................................................................123
9.4.6 Bottle Tubing...................................................................................................................125
9.4.7 Pump Tubing Replacement ............................................................................................127
9.4.8 Install Waste Modules.....................................................................................................130
9.4.9 Waste Tubing..................................................................................................................130
9.4.10 Install Sample Modules ............................................................................................131
9.4.11 Sipper Replacement.................................................................................................131
9.4.12 Calibrate Sipper........................................................................................................132
9.4.13 Install Bottles ............................................................................................................132
9.4.14 Install Membranes and ISEs.....................................................................................132
9.4.15 Prime Fluid System ..................................................................................................132

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Fuse Replacement.................................................................................................................1339.5 9.5.1 Fuse Requirements.........................................................................................................133
2960 Maintenance.................................................................................................................1339.6 9.6.1 Tubing Replacement.......................................................................................................133
9.6.2 Monitor Sample Cup .......................................................................................................135
10. Storage........................................................................................................................................136
Instrument Storage................................................................................................................13610.1
Enzyme Membrane Storage..................................................................................................13610.2
ISE Storage ...........................................................................................................................13610.310.3.1 Reference ISE ..........................................................................................................136
10.3.2 Ammonium/Potassium ISE.......................................................................................136
Instrument Handling/Transport..............................................................................................13610.4
11. Troubleshooting.........................................................................................................................136
Printout Information ...............................................................................................................13811.111.1.1 Enzyme Sensors ......................................................................................................138
11.1.2 Ion Selective Electrodes...........................................................................................140
Troubleshooting Chart...........................................................................................................14311.211.2.1 2960 Online Monitor .................................................................................................147
12. Principles of Operation..............................................................................................................149
Enzyme Sensor Technology..................................................................................................14912.1
Ion Selective Electrode..........................................................................................................15012.2
Measurement Methodology...................................................................................................15012.3
Baseline Stability ...................................................................................................................15112.4
Calibration..............................................................................................................................15112.5
Linearity.................................................................................................................................15112.6
Temperature Compensation..................................................................................................15212.7
Level Sensing........................................................................................................................15212.8
13. Warranty and Repair..................................................................................................................152
Limitation of Warranty............................................................................................................15213.113.1.1 Shipping Instructions ................................................................................................152
13.1.2 Cleaning Instructions................................................................................................153
YSI Factory Authorized Service Centers...............................................................................15413.2
14. Notices ........................................................................................................................................155
Declaration of Conformity......................................................................................................15514.1
Declaration of Conformity -Australian and New Zealand Electromagnetic Compatibility....15614.2
Radio and Television Interference Notice .............................................................................15714.3
15. Appendix A – Software Flowchart............................................................................................158
16. Appendix B – Concentration Unit Conversion........................................................................159
Linearity Test. Concentration Unit Conversion......................................................................16016.1
FCN Membrane Integrity Test. Concentration Unit Conversion............................................16116.2
17. Appendix C - Line Power Cord and Plug Wiring.....................................................................162
18. Appendix D - Reagents and Accessories................................................................................163

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1.Introduction
Description1.1
The 2900 Series Biochemistry Analyzer is a laboratory instrument intended for use in research, food-processing and
bioprocessing applications. THE 2900 Series IS NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN
PERFORMANCE EVALUATION.
The 2900 Series can be set up to measure up to 6 different analytes in a sample. The total number of analytes depends
on the number of sample modules installed (up to two analytes per module) and the number of different buffers required
(up to three buffers). Available analytes are listed below.
Ammonium

D-Glucose (Dextrose)
Potassium

Hydrogen Peroxide
1
L-Lactate
Sucrose
L-Glutamate
Ethanol
L-Glutamine

Glycerol
Ethanol-HC

Methanol
Xylose
Galactose1
Choline
Lactose
Additional analytes are currently under development. For a current listing, please contact YSI Life Sciences Technical
Support (937 767-2769 or 800 659-8895 extension 2) or visit the YSI Life Sciences web site at www.ysi.com/lifesciences.
User Features1.2
Slim modular design
Easily expand analytes or chemistries
Multiple units use much less bench space
Proprietary enzyme electrode
Fast, accurate, and analyte-specific results
Uses biological separation technology
No hazardous chromatography solvents to dispose
of
Icon-driven user interface with touchscreen
Easy to learn
Data download options
Save data on a USB drive, send it over the network,
or access it in a searchable database anytime
Onboard training videos
Minimizes operator learning curve
21 CFR Part 11
Regulatory compliance
Models1.3
•2900D 2900 Biochemistry Analyzer with 1 biosensor module
•2900M 2900 Online Monitor & Control System
•2950D-0 2950 Biochemistry Analyzer with 1 biosensor module
•2950D-1 2950 Biochemistry Analyzer with 2 biosensor modules
•2950D-2 2950 Biochemistry Analyzer with 3 biosensor modules
•2950D-3 2950 Biochemistry Analyzer with 2 biosensor modules and 1 ISE module
•2950D-4 2950 Biochemistry Analyzer with 1 biosensor module and 1 ISE module
1YSI does not currently offer calibration standards for this analyte.

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2900 Series General Specifications1.4
Sample size.................................................Adjustable from 10 to 50 microliters (aspirated volume), per module
Response time ............................................Enzyme Sensors
•Sample results in 60 seconds (average) for one module
•100 seconds for 2 modules
•135 seconds for 3 modules
•Complete sample-to-sample cycle in less than 3 minutes
(May vary with analyte and sample matrix.)
Ion Selective Electrodes (ISE)
•Sample results in 150 seconds (average) for 2 modules
•195 seconds for 3 modules
•Complete sample-to-sample cycle in less than 5 minutes
(May vary with analyte and sample matrix)
Output signals:
Serial.......................................................... USB and RS232
Power requirement ...............................100–240 VAC ±10%
50–60 Hz ±5%
42 Watts nominal
Working environment:
Ambient temperature................................15–35°C
Relative humidity......................................10–75% (non-condensing)
Regulatory compliance.................................. ETL, CE, RoHS
61010-1 compliance:
•Pollution degree 2
•Installation category 2
•Altitude 2000m
•Atmosphere 75 KPa to 106 KPa
•Indoor use only
Instrument dimensions ................................14.0" wide x 20.5" deep x 15.75" high
35.6 cm x 52.1 cm x 40.0 cm
Instrument weight........................................39 pounds
17.7 kilograms
2960 Online Monitor Specifications1.5
1.5.1 Monitor
Size:
Monitor Cup ............................................... 0.75 x 0.75 x 0.88 inches
Pump Head................................................ 2.0 x 2.2 x 0.85 inches
Sample Inlet Tubing ...................................... Silicone, 0.08 OD x 0.02 ID (inches)
Volume....................................................... 5.1 microliters/inch
Inlet Channel Pump Tubing........................... PharMed, 0.13 OD x 0.035 ID (inches)

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Valve Tubing ................................................. 0.03 ID (inches)
Wasteline Tubing .......................................... Silicone, 0.16 OD x 0.10 ID (inches)
Nominal Flow Rate (inlet line)....................... 100–2500 microliters/minute (±8% @ ±6 PSI)
1.5.2 Analog/Control
Full Scale Voltage ......................................... Selectable: +10.00 VDC or +5.00 VDC
Full Scale Concentration............................... User selectable
Resolution ..................................................... 1:65,536 or 0.0015% FS,
0.153 mv on +10.00 VFS,
0.076 mv on +5.00 VFS
Maximum Gain Error..................................... ±12 LSB
Linearity......................................................... ±1 LSB
Minimum analog output Load Impedance..... 2K Ohms
Logic output drive.......................................... 0 and 5 VDC nominal at 10 milliamps
Logic Input levels .......................................... < 0.8 VDC = logic 0,
...................................................................... > 2.0 VDC = logic 1
2.Safety
Important Safety Instructions2.1
DO NOT PLUG THE INSTRUMENT IN AT THIS TIME. You should apply power only when directed to do so in the setup
instructions.
1. Use ONLY the line power cord supplied with the instrument. When directed to, connect the plug to a matching three-
pronged wall receptacle.
2. Use ONLY fuses of the type supplied. Replacement power cords and fuses can be obtained from YSI, or your Dealer
Representative.
3. Do NOT use an extension cord without protective grounding.
4. Do NOT remove rear cover. There are no user serviceable parts inside.
5. Repairs are to be performed only by trained and approved personnel.
6. This instrument must be connected to a protectively grounded (earthed) outlet.
7. The following notice is provided in compliance with IEC1010 Part 1 1990.
See Appendix for mains plug wiring and fusing instructions.
8. If the equipment is used in a manner not specified by YSI, the protection provided by the equipment may be impaired.
WARNING: For RS232 or USB connection, equipment should be EN/CSA/UL 61010 or EN/CSA/UL 60950
approved only.
9. The mains (power) switch is for functional purposes ONLY. To disconnect the instrument from the mains supply,
unplug the mains power cord from the back of the instrument.
10. Personal protective equipment (PPE) recommended—protective gloves and safety goggles or glasses.

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Explanation of Symbols2.2
WARNING
AVERTISSEMENT
Warning indicates that misuse of the instrument could result in
death or serious injury to a person.
Un avertissement indique qu'une mauvaise utilisation de l'instrument
peut entraîner la mort ou une blessure grave chez une personne.
CAUTION
ATTENTION
Caution, consult accompanying documents. Caution indicates
that misuse of the instrument could result in mild or serious injury
to a person and/or damage to equipment.
Attention, consulter la documentation jointe. Cette mise en garde
indique qu'une mauvaise utilisation de l'instrument peut entraîner une
blessure légère ou grave chez une personne et/ou un endommagement
du matériel.
Biological Risks
Risques biologiques
Chemical Irritant
Irritant chimique
Manufacturer
Fabricant
Authorized Representative in the European Union
Représentant agréé dans l'Union européenne
2776
Catalog number
Numéro de référence
15A100549
Lot number
Numéro de lot
YEAR-MO
Date of manufacture
Date de fabrication
YEAR-MO
Use by Date
Date limite d'utilisation
Temperature Limitation
Limite de température
REF

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3.Getting Started
Unpacking3.1
When you unpack your new 2900 Series for the first time, check the packing list to make sure you have received
everything listed. Note that reagents for the 2900 Series are not packaged in the same carton as the instrument. If there is
anything missing or damaged, call the dealer from whom you purchased the 2900 Series. If you do not know which of our
authorized dealers sold the system to you, call YSI Life Sciences Customer Service at 800 659-8895 or 937 767-7241,
and we'll be happy to help you.
1. After removing the instrument from the shipping box, tilt the display to the full upright position.
Figure 3.1
2. Grasp the hand hold in the right side cover of the instrument and pull up and out to remove the cover.
NOTE: Leave the cover off the instrument until you have aligned the sipper and installed the membranes as
described in the following sections.
3. Carefully cut the tie strap holding the sipper.
Figure 3.2
Remove tie strap

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Warranty Card3.2
Please complete the Warranty Card and return it to YSI. This will record your purchase of this instrument in our computer
system. Once your purchase is recorded, you will receive prompt, efficient service in the event any part of your 2900
Series should ever need repair.
What You Need3.3
Several things are needed in order to analyze samples using the 2900 Series. The following list shows the basic items
required.
•2900 Series Instrument (with AC Power Cord)
•Bottle Racks with Reagent Level Sensing (YSI 2936, 2938), or Bottles without Reagent Level Sensing (YSI
2934, 2937)
•YSI Buffer(s)
•YSI Calibrator Standard(s)
•YSI Linearity Standard(s)
•YSI Membrane(s)
•YSI ISE probes (2950D-3 and 2950D-4 models only)
•YSI 2960 Online Monitor (optional)
•YSI 2901 Printer (optional)
Major Components3.4
Figure 3.3
Display Graphical color LCD covered by a touch screen
Cover
Sipper
Display/
Touch Panel
USB Port
Station 2
Bottle Rack
Station 1

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USB ports The USB ports allow a flash drive to be connected to the 2900
Series to download sample results or upgrade the instrument’s
software. A USB port is located on the right side of the display. An
additional USB port is located on the rear of the instrument. The rear
port is used to connect the 2960 Online Monitor to the 2900 Series.
Sipper Can be raised, lowered, rotated, and moved horizontally to its
various positions.
The positions are: Calibrator Wells, Sample Module, Stations 1 and
2, and Monitor Sample Cup.
The Sipper senses fluid level to control immersion depth and detect
errors.
Station 1 Plate and rack holder accepts most standard plates/racks for batch
sampling of up to 96 samples.
Station 2 Test tube holder for manual sampling.
Buffer Pumps
(not shown) Draw buffer from the buffer bottles, pump it through the Valves,
Sipper Pump and the Sipper, and flush the Sample Module.
Calibrator Pumps
(not shown) Draw the appropriate standard solution from the Calibrator Bottles
and fill the Calibrator Wells.
Figure 3.4
Sipper pump It retracts its piston to draw standard from the Calibrator Wells or
sample from the sample stations.
It extends its piston to dispense standard or sample into the sample
module.
Sample modules They are made of clear acrylic plastic. Sensor probes are screwed
into either side of the module.
Sample Module
Sipper Pump
Waste Bottle
Buffer Bottle
Calibrator Bottles

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In biosensor modules, the immobilized enzyme membranes are
mounted on O-rings which act as fluid seals.
In ISE modules, an O-ring is positioned inside a sleeve on the end of
the electrode.
A reference or auxiliary electrode is housed in the temperature probe
and positioned at the back of the Sample module.
Stir Bars
(not shown) They are plastic encapsulated magnets. They are activated by
motors housed below the sample modules. They provide thorough
mixing inside the sample modules.
Buffer, Waste and
Calibrator Bottles Are conveniently located for maintenance.
Fluid levels are monitored by sensors.
Operation is automatically halted when the Buffer or Calibrator
Bottles are empty, or when the Waste bottles are full.
Rear Panel
Figure 3.5
Power Switch The main power switch is an on/off rocker switch (0-off and I-on)
located on the back of the instrument
Fuse Holder
The fuse holder houses the power line fuse and opens up for fuse
replacement.
Power Receptacle One end of the power cord (supplied) plugs into this receptacle,
while the other end plugs into a properly grounded electrical outlet.
The instrument will automatically adjust the voltage as needed.
Ethernet Port It allows connection to a network or router via a RJ45 Ethernet port.
RS232 Serial Port The RS232 connection is a standard DB9F connector. It is used to
interface with the YSI 2901 Printer or a remote host.
4.Basic Setup
The following list describes the basic steps necessary for sampling with the 2900 Series.
1. Install Bottle Racks
2. Connect Printer (optional)
3. Connect AC Power
Power
Receptacle
RS232 Port
Power Switch
Fuse
Holder
Ethernet Port
USB Port

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4. Connect 2960 Online Monitor (optional)
5. Align Sipper
6. Configure Instrument Chemistries
7. Prepare and Install Buffer solution(s)
8. Install Calibrator Solution(s)
9. Install Membrane(s) and ISEs
10. Prime the Fluid System
11. Check Probe Currents
Install Bottle Racks4.1
4.1.1 Right Side
1. Install the YSI 2938 Bottle Rack with Reagent Level Sensing onto the right side of the instrument by sliding the
slots in the tray over the pins on the side of the instrument.
If you are not using the reagent level sensing option, place the YSI 2934 Bottles on the right side of the
instrument.
2. Then remove the packing material holding the tubing to the right side of the instrument.
3. Next, connect bottle tubing and cables
a. Insert the large diameter waste tube into the hole in waste bottle 1.
b. Connect one end of a short cable marked to the threaded fitting on the waste bottle 1 cap and connect
the other end to the Waste 1 (top) fitting on the instrument.
c. Connect the tubing marked “B1” and one end of a short cable to the fittings on the buffer bottle 1 cap and
connect the other end of the cable to the Buffer 1 (2nd) fitting on the instrument.
d. Connect the tubing marked “C1B” and one end of a long cable to the fittings on the second calibrator
bottle cap and connect the other end of the cable to the CAL 1B (3rd) fitting on the instrument.
e. Connect the tubing marked “C1A” and one end of a long cable to the fittings on the first calibrator bottle
cap and connect the other end of the cable to the CAL 1A (bottom) fitting on the instrument.
Figure 4.1
Buffer Bottle 1
Waste Bottle
Calibrator Bottle
CAL 1B
Waste 1
Buffer 1
CAL 1A
CAL 1B
Calibrator Bottle
CAL 1A

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4.1.2 Left Side (2950 models only)
1. Install the YSI 2936 Bottle Rack with Reagent Level Sensing onto the left side of the instrument by sliding the
slots in the tray over the pins on the side of the instrument.
2. If you are not using the reagent level sensing option, place the YSI 2937 Bottles on the left side of the instrument.
3. Then remove the packing material holding the tubing to the left side of the instrument.
4. Next, connect bottle tubing and cables
a. Insert the large diameter waste tubing into the holes in waste bottles 2 and 3.
b. Connect one end of a long cable to the threaded fitting on the waste bottle 2 cap and connect the other
end to the W2 (top left) fitting on the instrument.
c. Connect one end of a long cable to the threaded fitting on the waste bottle 3 cap and connect the other
end to the W3 (top right) fitting on the instrument.
d. Connect the tubing marked “B2” and one end of a long cable to the fittings on the buffer 2 bottle cap and
connect the other end of the cable to the B2 (2nd from top on left) fitting on the instrument.
e. Connect the tubing marked “B3” and one end of a long cable to the fittings on the buffer 3 bottle cap and
connect the other end of the cable to the B3 (2nd from top on right) fitting on the instrument.
f. Connect the tubing marked “C2A” and one end of a short cable to the fittings on the calibrator 2A bottle
cap and connect the other end of the cable to the 2A (bottom left) fitting on the instrument.
g. Connect the tubing marked “C2B” and one end of a short cable to the fittings on the calibrator 2B bottle
cap and connect the other end of the cable to the 2B (3rd from top on left) fitting on the instrument.
h. Connect the tubing marked “C3A” and one end of a short cable to the fittings on the calibrator 3A bottle
cap and connect the other end of the cable to the 3A (bottom right) fitting on the instrument.
i. Connect the tubing marked “C3B” and one end of a short cable to the fittings on the calibrator 3B bottle
cap and connect the other end of the cable to the 3B (3rd from top on right) fitting on the instrument.
Figure 4.2
Buffer Bottle 2
Waste Bottle 3
Waste Bottle 2
Calibrator Bottle
CAL 3A
Buffer Bottle 3
Calibrator Bottle
CAL 2A

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Connect Printer4.2
Connect the YSI 2901 Printer to the 2900 Series Biochemistry Analyzer using the data cable provided. The small RJ12
connector plugs into the bottom of the printer and the large DB9 connector plugs into the RS232 port on the back of the
analyzer. Refer to the instruction sheet included with the printer for details of printer operation.
Connect AC Power4.3
1. Plug the power cord (included with the 2900 Series Analyzer) into the power receptacle on the back of the
instrument, then into a properly grounded electrical outlet provided with a 15 or 20 Amp circuit breaker. The
instrument will automatically adjust the voltage as needed.
If you are located outside the United States, see Appendix for Line Power Cord and Plug Wiring.
WARNING: Keep your hands clear of the sipper while the instrument is in operation.
2. Turn the instrument on with the main power switch on the rear panel. After about 30 seconds, the Initializing
window should appear.
3. The first time the instrument is powered up, the software license window will appear. Touch [X] to prevent the
license screen from appearing each time the instrument is turned on.
4. Since the top cover of the instrument is removed, the Service screen will appear. Touch the Interlock [On] button
and change it to [Off] to disable the safety interlock, and then press to confirm.

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Connect 2960 Online Monitor4.4
If you will be using the 2960 Online Monitor, see Section 6.1 for instructions on how to connect it.
Align Sipper4.5
It is very important that the sipper be accurately adjusted.
WARNING: Keep your hands clear of the sipper while the instrument is in operation.
1. From the Sipper tab of the Service screen, touch [Module 1].
2. The Select Location screen will appear. Select [Module 1]. The sipper will move to sample module 1 and should
be centered above the cone shaped opening in the top of the module.
If the sipper does not move, make sure the packing material was removed.

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3. If the sipper is not centered, touch [Position] and use the arrow buttons to center the sipper.
4. Make certain the Sipper is centered, then touch at the bottom right of the adjustment window.
Sipper Adjustment Position
Figure 4.3
5. Touch to save the position and close the confirmation window.
6. To test the alignment of the sipper, Touch [Inject] to lower the sipper, then touch [Retract] to raise the sipper back
up.
If necessary, touch [Position] and repeat the adjustment.

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7. Once the sipper enters the sample module without hitting the cone, touch [Depth] to set the sipper depth. The
Select Location screen will appear.
8. Select [Module 1]. The tip of the sipper should be right at the top of the module. Use the arrow buttons to lower or
raise the sipper until the tip of the sipper is even with the top of the module.
9. Then touch at the bottom right of the adjustment window.
10. Touch to save the depth and close the confirmation window.
11. Check the sipper alignment at the Cal 1A, Cal 1B and Drain 1 locations and adjust the position if necessary.

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12. Once you have aligned the sipper and properly set the depth, sipper alignment for Module 1 is complete. Touch
[X] at the top left of the screen to return to the main display.
Repeat this procedure for any additional modules installed on your 2950 analyzer.
After you have aligned the sipper with all installed modules, touch the Interlock button and change it back to [On]
to enable the safety interlocks.
Configure Instrument Chemistries4.6
Before operating the 2900 Series, you must set the instrument parameters.
4.6.1 Assign Chemistries to Probes
1. From the main display, touch .
2. From the [Module 1] tab, touch the Probe A membrane button.
3. Select the chemistry you want to measure