YSI 2900 series User manual

YSI 2900 Series
Biochemistry Analyzers
Operations and Maintenance Manual

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Table of Contents
1. Introduction ....................................................................................................................................5
Description.................................................................................................................................51.1
User Features............................................................................................................................51.2
Models .......................................................................................................................................51.3
2900 Series General Specifications ..........................................................................................61.4
2960 Online Monitor Specifications...........................................................................................61.5 1.5.1 Monitor ................................................................................................................................6
1.5.2 Analog/Control ....................................................................................................................7
2. Safety...............................................................................................................................................7
Important Safety Instructions.....................................................................................................72.1
Explanation of Symbols.............................................................................................................82.2
3. Getting Started ...............................................................................................................................9
Unpacking..................................................................................................................................93.1
Warranty Card .........................................................................................................................103.2
What You Need .......................................................................................................................103.3
Major Components ..................................................................................................................103.4
4. Basic Setup...................................................................................................................................12
Install Bottle Racks..................................................................................................................134.1 4.1.1 Right Side..........................................................................................................................13
4.1.2 Left Side (2950 models only) ............................................................................................14
Connect Printer........................................................................................................................154.2
Connect AC Power..................................................................................................................154.3
Connect 2960 Online Monitor..................................................................................................164.4
Align Sipper .............................................................................................................................164.5
Configure Instrument Chemistries...........................................................................................194.6 4.6.1 Assign Chemistries to Probes...........................................................................................19
4.6.2 Assign Reagents...............................................................................................................22
Prepare and Install Buffer Solutions........................................................................................244.7 4.7.1 Prepare Buffer...................................................................................................................25
4.7.2 Install Buffer Solution(s)....................................................................................................25
Install Calibrator Solution(s) ....................................................................................................254.8
Install Membranes and ISEs....................................................................................................264.9 4.9.1 Enzyme Membranes.........................................................................................................26
4.9.2 Ion Selective Electrodes....................................................................................................27
Prime the Fluid System ...........................................................................................................294.10
Check Probe Currents.............................................................................................................304.114.11.1 Biosensor Probes .......................................................................................................30
4.11.2 ISE Probes..................................................................................................................30
Enable 21 CFR Part 11 Mode .................................................................................................304.12
5. Running the Instrument...............................................................................................................30
Perform Daily Operational Checks..........................................................................................305.1 5.1.1 Enzyme Membrane Integrity Test.....................................................................................31
5.1.2 Linearity Test.....................................................................................................................33
5.1.3 Results ..............................................................................................................................33
Sample Preparation.................................................................................................................355.2
Run Batch................................................................................................................................355.3

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5.3.1 Create Batches .................................................................................................................35
5.3.2 Load Samples ...................................................................................................................37
5.3.3 Start...................................................................................................................................39
5.3.4 Status................................................................................................................................39
Run Stat...................................................................................................................................395.4
Results.....................................................................................................................................425.5
6. Online Monitor and Control.........................................................................................................42
2960 Installation.......................................................................................................................436.1
Align Sipper .............................................................................................................................466.2
Sample Interface......................................................................................................................466.3 6.3.1 Sterilization........................................................................................................................47
Electrical Interface...................................................................................................................476.4 6.4.1 Analog Outputs .................................................................................................................47
6.4.2 Pump Control Outputs.......................................................................................................48
6.4.3 Auxiliary Connector / Signal List .......................................................................................48
Monitor Setup ..........................................................................................................................486.5 6.5.1 Name.................................................................................................................................49
6.5.2 Flow Rate and Purge Time ...............................................................................................50
6.5.3 Antiseptic...........................................................................................................................50
6.5.4 Filtrate Pump.....................................................................................................................51
6.5.5 Module...............................................................................................................................51
Start Monitor............................................................................................................................566.6 6.6.1 Manual Sample during Monitor Session...........................................................................57
Stop Monitor ............................................................................................................................586.7
Control Setup...........................................................................................................................586.8 6.8.1 Control Type......................................................................................................................58
Print Monitor Configuration......................................................................................................676.9
7. System Configuration..................................................................................................................68
Settings....................................................................................................................................687.1 7.1.1 System ..............................................................................................................................68
7.1.2 Fluid Detection (Bottles)....................................................................................................70
7.1.3 Display...............................................................................................................................71
7.1.4 Auto-Cal Settings ..............................................................................................................73
7.1.5 Scheduler..........................................................................................................................74
7.1.6 21 CFR Part 11 .................................................................................................................76
7.1.7 Date/Time..........................................................................................................................81
Service.....................................................................................................................................827.2 7.2.1 Sipper................................................................................................................................82
7.2.2 Pumps...............................................................................................................................85
7.2.3 Modules.............................................................................................................................86
7.2.4 Stirbar................................................................................................................................87
7.2.5 Monitor ..............................................................................................................................89
Data .........................................................................................................................................907.3 7.3.1 Plate..................................................................................................................................90
7.3.2 Monitor ..............................................................................................................................92
7.3.3 Calibration.........................................................................................................................94
Help .........................................................................................................................................957.4 7.4.1 About.................................................................................................................................95
7.4.2 Software............................................................................................................................96
7.4.3 FAQ...................................................................................................................................97
7.4.4 Training .............................................................................................................................97

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Ethernet Port............................................................................................................................987.5 7.5.1 LAN (Shared Network Connection)...................................................................................98
7.5.2 Router (Private Network Connection) ...............................................................................98
7.5.3 Accessing Stored Data......................................................................................................99
8. Chemistry Setup.........................................................................................................................101
Sample Volume .....................................................................................................................1018.1
Measurement Parameter Information....................................................................................1018.2 8.2.1 Choline............................................................................................................................102
8.2.2 Ethanol/Ethanol-HC ........................................................................................................103
8.2.3 Galactose........................................................................................................................104
8.2.4 D-Glucose (Dextrose) .....................................................................................................105
8.2.5 L-Glutamate (L-Glutamic Acid) .......................................................................................106
8.2.6 L-Glutamine.....................................................................................................................107
8.2.7 Glycerol...........................................................................................................................108
8.2.8 Hydrogen Peroxide .........................................................................................................109
8.2.9 L-Lactate .........................................................................................................................110
8.2.10 Lactose.....................................................................................................................111
8.2.11 Methanol...................................................................................................................112
8.2.12 Sucrose.....................................................................................................................113
8.2.13 Xylose.......................................................................................................................114
8.2.14 Simultaneous Ammonium and Potassium................................................................115
8.2.15 Simultaneous Glucose and L-Lactate ......................................................................116
8.2.16 Simultaneous Glucose and Sucrose ........................................................................117
8.2.17 Simultaneous Glucose and Xylose...........................................................................118
8.2.18 Simultaneous L-Glutamate and L-Glutamine...........................................................119
9. Operational Checks and Maintenance.....................................................................................119
Cleaning, Disinfecting, and Decontaminating........................................................................1199.1 9.1.1 Touch Panel....................................................................................................................119
9.1.2 Decontamination Procedures..........................................................................................119
Daily Maintenance.................................................................................................................1209.2 9.2.1 Empty the Waste Bottle(s) ..............................................................................................120
9.2.2 Check the Calibrator Bottle(s).........................................................................................120
9.2.3 Check the Buffer Bottle(s)...............................................................................................120
9.2.4 Check for Leaks..............................................................................................................120
9.2.5 Clean up Spills ................................................................................................................120
9.2.6 Daily Operational Checks................................................................................................120
Monthly Maintenance ............................................................................................................1209.3 9.3.1 Calibration Pumping System Maintenance.....................................................................120
Preventive Maintenance – 6 months or 1000 Hours.............................................................1219.4 9.4.1 Sample Module Cleaning................................................................................................121
9.4.2 Waste Module Cleaning..................................................................................................122
9.4.3 Enzyme Probe Cleaning .................................................................................................122
9.4.4 ISE Cleaning ...................................................................................................................123
9.4.5 Sipper pump Seal Replacement.....................................................................................123
9.4.6 Bottle Tubing...................................................................................................................125
9.4.7 Pump Tubing Replacement ............................................................................................127
9.4.8 Install Waste Modules.....................................................................................................130
9.4.9 Waste Tubing..................................................................................................................130
9.4.10 Install Sample Modules ............................................................................................131
9.4.11 Sipper Replacement.................................................................................................131
9.4.12 Calibrate Sipper........................................................................................................132
9.4.13 Install Bottles ............................................................................................................132
9.4.14 Install Membranes and ISEs.....................................................................................132
9.4.15 Prime Fluid System ..................................................................................................132

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Fuse Replacement.................................................................................................................1339.5 9.5.1 Fuse Requirements.........................................................................................................133
2960 Maintenance.................................................................................................................1339.6 9.6.1 Tubing Replacement.......................................................................................................133
9.6.2 Monitor Sample Cup .......................................................................................................135
10. Storage........................................................................................................................................136
Instrument Storage................................................................................................................13610.1
Enzyme Membrane Storage..................................................................................................13610.2
ISE Storage ...........................................................................................................................13610.310.3.1 Reference ISE ..........................................................................................................136
10.3.2 Ammonium/Potassium ISE.......................................................................................136
Instrument Handling/Transport..............................................................................................13610.4
11. Troubleshooting.........................................................................................................................136
Printout Information ...............................................................................................................13811.111.1.1 Enzyme Sensors ......................................................................................................138
11.1.2 Ion Selective Electrodes...........................................................................................140
Troubleshooting Chart...........................................................................................................14311.211.2.1 2960 Online Monitor .................................................................................................147
12. Principles of Operation..............................................................................................................149
Enzyme Sensor Technology..................................................................................................14912.1
Ion Selective Electrode..........................................................................................................15012.2
Measurement Methodology...................................................................................................15012.3
Baseline Stability ...................................................................................................................15112.4
Calibration..............................................................................................................................15112.5
Linearity.................................................................................................................................15112.6
Temperature Compensation..................................................................................................15212.7
Level Sensing........................................................................................................................15212.8
13. Warranty and Repair..................................................................................................................152
Limitation of Warranty............................................................................................................15213.113.1.1 Shipping Instructions ................................................................................................152
13.1.2 Cleaning Instructions................................................................................................153
YSI Factory Authorized Service Centers...............................................................................15413.2
14. Notices ........................................................................................................................................155
Declaration of Conformity......................................................................................................15514.1
Declaration of Conformity -Australian and New Zealand Electromagnetic Compatibility....15614.2
Radio and Television Interference Notice .............................................................................15714.3
15. Appendix A – Software Flowchart............................................................................................158
16. Appendix B – Concentration Unit Conversion........................................................................159
Linearity Test. Concentration Unit Conversion......................................................................16016.1
FCN Membrane Integrity Test. Concentration Unit Conversion............................................16116.2
17. Appendix C - Line Power Cord and Plug Wiring.....................................................................162
18. Appendix D - Reagents and Accessories................................................................................163

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1.Introduction
Description1.1
The 2900 Series Biochemistry Analyzer is a laboratory instrument intended for use in research, food-processing and
bioprocessing applications. THE 2900 Series IS NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN
PERFORMANCE EVALUATION.
The 2900 Series can be set up to measure up to 6 different analytes in a sample. The total number of analytes depends
on the number of sample modules installed (up to two analytes per module) and the number of different buffers required
(up to three buffers). Available analytes are listed below.
Ammonium

D-Glucose (Dextrose)
Potassium

Hydrogen Peroxide
1
L-Lactate
Sucrose
L-Glutamate
Ethanol
L-Glutamine

Glycerol
Ethanol-HC

Methanol
Xylose
Galactose1
Choline
Lactose
Additional analytes are currently under development. For a current listing, please contact YSI Life Sciences Technical
Support (937 767-2769 or 800 659-8895 extension 2) or visit the YSI Life Sciences web site at www.ysi.com/lifesciences.
User Features1.2
Slim modular design
Easily expand analytes or chemistries
Multiple units use much less bench space
Proprietary enzyme electrode
Fast, accurate, and analyte-specific results
Uses biological separation technology
No hazardous chromatography solvents to dispose
of
Icon-driven user interface with touchscreen
Easy to learn
Data download options
Save data on a USB drive, send it over the network,
or access it in a searchable database anytime
Onboard training videos
Minimizes operator learning curve
21 CFR Part 11
Regulatory compliance
Models1.3
•2900D 2900 Biochemistry Analyzer with 1 biosensor module
•2900M 2900 Online Monitor & Control System
•2950D-0 2950 Biochemistry Analyzer with 1 biosensor module
•2950D-1 2950 Biochemistry Analyzer with 2 biosensor modules
•2950D-2 2950 Biochemistry Analyzer with 3 biosensor modules
•2950D-3 2950 Biochemistry Analyzer with 2 biosensor modules and 1 ISE module
•2950D-4 2950 Biochemistry Analyzer with 1 biosensor module and 1 ISE module
1YSI does not currently offer calibration standards for this analyte.

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2900 Series General Specifications1.4
Sample size.................................................Adjustable from 10 to 50 microliters (aspirated volume), per module
Response time ............................................Enzyme Sensors
•Sample results in 60 seconds (average) for one module
•100 seconds for 2 modules
•135 seconds for 3 modules
•Complete sample-to-sample cycle in less than 3 minutes
(May vary with analyte and sample matrix.)
Ion Selective Electrodes (ISE)
•Sample results in 150 seconds (average) for 2 modules
•195 seconds for 3 modules
•Complete sample-to-sample cycle in less than 5 minutes
(May vary with analyte and sample matrix)
Output signals:
Serial.......................................................... USB and RS232
Power requirement ...............................100–240 VAC ±10%
50–60 Hz ±5%
42 Watts nominal
Working environment:
Ambient temperature................................15–35°C
Relative humidity......................................10–75% (non-condensing)
Regulatory compliance.................................. ETL, CE, RoHS
61010-1 compliance:
•Pollution degree 2
•Installation category 2
•Altitude 2000m
•Atmosphere 75 KPa to 106 KPa
•Indoor use only
Instrument dimensions ................................14.0" wide x 20.5" deep x 15.75" high
35.6 cm x 52.1 cm x 40.0 cm
Instrument weight........................................39 pounds
17.7 kilograms
2960 Online Monitor Specifications1.5
1.5.1 Monitor
Size:
Monitor Cup ............................................... 0.75 x 0.75 x 0.88 inches
Pump Head................................................ 2.0 x 2.2 x 0.85 inches
Sample Inlet Tubing ...................................... Silicone, 0.08 OD x 0.02 ID (inches)
Volume....................................................... 5.1 microliters/inch
Inlet Channel Pump Tubing........................... PharMed, 0.13 OD x 0.035 ID (inches)

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Valve Tubing ................................................. 0.03 ID (inches)
Wasteline Tubing .......................................... Silicone, 0.16 OD x 0.10 ID (inches)
Nominal Flow Rate (inlet line)....................... 100–2500 microliters/minute (±8% @ ±6 PSI)
1.5.2 Analog/Control
Full Scale Voltage ......................................... Selectable: +10.00 VDC or +5.00 VDC
Full Scale Concentration............................... User selectable
Resolution ..................................................... 1:65,536 or 0.0015% FS,
0.153 mv on +10.00 VFS,
0.076 mv on +5.00 VFS
Maximum Gain Error..................................... ±12 LSB
Linearity......................................................... ±1 LSB
Minimum analog output Load Impedance..... 2K Ohms
Logic output drive.......................................... 0 and 5 VDC nominal at 10 milliamps
Logic Input levels .......................................... < 0.8 VDC = logic 0,
...................................................................... > 2.0 VDC = logic 1
2.Safety
Important Safety Instructions2.1
DO NOT PLUG THE INSTRUMENT IN AT THIS TIME. You should apply power only when directed to do so in the setup
instructions.
1. Use ONLY the line power cord supplied with the instrument. When directed to, connect the plug to a matching three-
pronged wall receptacle.
2. Use ONLY fuses of the type supplied. Replacement power cords and fuses can be obtained from YSI, or your Dealer
Representative.
3. Do NOT use an extension cord without protective grounding.
4. Do NOT remove rear cover. There are no user serviceable parts inside.
5. Repairs are to be performed only by trained and approved personnel.
6. This instrument must be connected to a protectively grounded (earthed) outlet.
7. The following notice is provided in compliance with IEC1010 Part 1 1990.
See Appendix for mains plug wiring and fusing instructions.
8. If the equipment is used in a manner not specified by YSI, the protection provided by the equipment may be impaired.
WARNING: For RS232 or USB connection, equipment should be EN/CSA/UL 61010 or EN/CSA/UL 60950
approved only.
9. The mains (power) switch is for functional purposes ONLY. To disconnect the instrument from the mains supply,
unplug the mains power cord from the back of the instrument.
10. Personal protective equipment (PPE) recommended—protective gloves and safety goggles or glasses.

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Explanation of Symbols2.2
WARNING
AVERTISSEMENT
Warning indicates that misuse of the instrument could result in
death or serious injury to a person.
Un avertissement indique qu'une mauvaise utilisation de l'instrument
peut entraîner la mort ou une blessure grave chez une personne.
CAUTION
ATTENTION
Caution, consult accompanying documents. Caution indicates
that misuse of the instrument could result in mild or serious injury
to a person and/or damage to equipment.
Attention, consulter la documentation jointe. Cette mise en garde
indique qu'une mauvaise utilisation de l'instrument peut entraîner une
blessure légère ou grave chez une personne et/ou un endommagement
du matériel.
Biological Risks
Risques biologiques
Chemical Irritant
Irritant chimique
Manufacturer
Fabricant
Authorized Representative in the European Union
Représentant agréé dans l'Union européenne
2776
Catalog number
Numéro de référence
15A100549
Lot number
Numéro de lot
YEAR-MO
Date of manufacture
Date de fabrication
YEAR-MO
Use by Date
Date limite d'utilisation
Temperature Limitation
Limite de température
REF

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3.Getting Started
Unpacking3.1
When you unpack your new 2900 Series for the first time, check the packing list to make sure you have received
everything listed. Note that reagents for the 2900 Series are not packaged in the same carton as the instrument. If there is
anything missing or damaged, call the dealer from whom you purchased the 2900 Series. If you do not know which of our
authorized dealers sold the system to you, call YSI Life Sciences Customer Service at 800 659-8895 or 937 767-7241,
and we'll be happy to help you.
1. After removing the instrument from the shipping box, tilt the display to the full upright position.
Figure 3.1
2. Grasp the hand hold in the right side cover of the instrument and pull up and out to remove the cover.
NOTE: Leave the cover off the instrument until you have aligned the sipper and installed the membranes as
described in the following sections.
3. Carefully cut the tie strap holding the sipper.
Figure 3.2
Remove tie strap