Zeiss Axiocam 105 color Installation and operating manual

Axiocam 105 color Installation + Reference –10.2013
October 2013

Carl Zeiss draws the User's attention to the fact that the information and references
contained in these documents may be subject to technical modifications, in
particular due to the continuous further development of Carl Zeiss's products. The
documents enclosed do not contain any warranty by Carl Zeiss with regard to the
technical processes described in the documentation or to certain reproduced
product characteristics. Furthermore, Carl Zeiss shall not be held liable for any
possible printing errors or other inaccuracies in this documentation, unless proof
can be furnished that any such errors or inaccuracies are already known by Carl
Zeiss or that these are not known to Carl Zeiss due to gross negligence and that
furthermore Carl Zeiss has for these reasons refrained from eliminating these errors
or inaccuracies appropriately. Carl Zeiss hereby explicitly draws the User's attention
to the fact that this manual only contains a general description of the technical
processes and information, the implementation of which in any individual case may
not be appropriate in the form described here. In cases of doubt, we recommend
the User to consult Carl Zeiss. This manual is protected by copyright. Carl Zeiss
has reserved all rights to this documentation. It is prohibited to make copies, partial
copies, or to translate this manual into any other language, except copies for
personal use. Carl Zeiss explicitly draws attention to the fact that the information
contained in this manual will be updated regularly in compliance with the technical
modifications and supplements carried out in the products and furthermore that this
manual only reflects the technical status of Carl Zeiss's products at the time of
printing. Microsoft, Windows, and Windows 7 are registered trademarks of Microsoft
Corporation in the United States and/or other countries.
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07740 Jena, Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen, Germany
© Carl Zeiss Microscopy GmbH, 2013
authorized dealer:
Pulch + Lorenz microscopy
Am Untergrün 23, -79232 March
tel: [phone]
fax: [phone]
mail: [email protected]
web: pulchlorenz.de

Notice from the Producer
The referenced Software of Carl Zeiss ("Zeiss Software"), contained in this
documentation, was designed, realized, verified, validated and released in
a certificated process environment. The quality management system is
certified following DIN EN ISO 9001.
The fields of application of the Software are common tasks and
applications in microscopy and/or imaging (so called "Off-The-Shelf
Software"). Though the user acknowledges that in any kind of use the end
user of the Software is responsible for the validation of the Software for the
end user’s dedicated intent of use considering all requirements of law and
standards (e. g. 21 CFR part 11, FDA, MDD etc.). If necessary the end
user has to establish, document, implement and maintain a special process
to fulfill all the requirements to conform with the valid rules of law and
standards.
CARL ZEISS DOES NOT WARRANT THAT THIS SOFTWARE IS
USABLE FOR SPECIAL PURPOSES OTHER THAN IN THE FIELDS OF
APPLICATION DEFINED ABOVE.
Safety
Refer to the safety notes and instructions in the manuals of
all necessary devices (e.g. microscope peripherals,
cameras, computers, computer additionals, etc.) before
installing and using the software (see also "Safety Notes
AxioVision, AxioVision LE, SAP 000000-2054-368").

Disposal and Recycling
This product has been developed, tested and manufactured
in accordance with the applicable environmental provisions
and directives of the European Union.
The product and its accessories comply with EU directives
2002/95/EC (RoHS) and 2002/96/EC (WEEE), insofar as these
apply to this product.
We have implemented a take-back and recycling process that
ensures that proper recycling is carried out in accordance with the
aforementioned EU directives.
Please contact your Carl Zeiss sales/service organization for
details relating to disposal and recycling.
This product must not be disposed of with domestic waste or using
municipal waste disposal services. In the event of resale, the seller
must inform the buyer of the need to dispose of the product
appropriately.

Table of Contents
Axiocam 105 color Installation + Reference Guide i
Table of Contents
1. Installation............................................................................................... 1
1.1 Safety Regulations........................................................................ 1
General Notes...........................................................................3
Backup Copies..........................................................................3
1.2 System Requirements...................................................................4
Contents of Delivery..................................................................4
1.3 Setting up the Camera System ..................................................... 5
Operating the Camera on a PC.................................................5
Installing the PCIe bus interface card on the PC.................5
Connecting the camera to the PC........................................6
Mounting the camera onto the microscope..........................7
Function Indicator......................................................................7
Checking the Equipment has been set up correctly..................8
1.4 Installing the Driver Software ........................................................8
Installing the Camera Driver using the Setup Program.............8
1.5 Notes on Maintenance ................................................................11
Camera Electronic...................................................................11
LED Indications.......................................................................11
Optical System........................................................................11
Cleaning the Infrared Barrier Filter or Protective Lens............11
Important Note on the Use of C-Mount Objectives .................12
2. Image Acquisition................................................................................. 13
2.1 General........................................................................................13
2.2 Quick Guide to the First Image with AxioVision..........................13
The Standard Workflow...........................................................14
Step by Step to the First Image...............................................15
2.3 Adjust Property Page ..................................................................21
Exposure .................................................................................22
Automatic Exposure...........................................................23
White Balance.........................................................................23
Color Adjustment................................................................24
Histogram................................................................................25
2.4 Frame Property Page..................................................................27
Camera Mode..........................................................................27
Frame......................................................................................28

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2.5 General Property Page................................................................30
Color Saturation ......................................................................30
Image Orientation....................................................................31
Shading Correction..................................................................31
2.6 Practical notes on Operation in AxioVision .................................35
Reset Camera to a Default State............................................35
Notes on Optimum Color Reproduction in AxioVision ............37
2.7 Information for Displaying Axiocam Images in AxioVision..........38
Recommended Camera Settings............................................40
Recommended Monitor Settings.............................................41
2.8 Background Information..............................................................41
Gamma Setting .......................................................................41
Configurable Toolbars for Operating the Camera...................41
3. Trouble Shooting..................................................................................42
3.1 Software ......................................................................................42
4. Technical Data ...................................................................................... 44
4.1 Axiocam 105 color.......................................................................44

Installation
Axiocam 105 color Installation + Reference Guide 1
1. Installation
The Axiocam 105 color is a high-resolution digital camera with a color
CMOS sensor and USB 3.0 interface for universal light-microscopy
applications.
To make it easier for you to set up the camera, please follow the
instructions in these chapters step by step.
In this manual the interface between the Axiocam 105 color and the
computer is called USB 3.0.
1.1 Safety Regulations
Please read this chapter carefully and observe the regulations in
order to ensure your safety and the intended operation of the
system. Please observe the warnings and notes printed in this
manual and on the unit.
The cameras has been manufactured and tested by Carl Zeiss according to
the regulations specified in CE and has left the manufacturer’s premises in
perfect working order.
In order to ensure that this condition is maintained and to avoid any risks
when operating the system, the user must comply with any notes and
warnings contained in this manual.
Exemption from statutory liability for accidents
The manufacturer shall be exempt from statutory liability for accidents
should the operator fail to observe the safety regulations.

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Limitation of liability
No warranty shall be assumed by Carl Zeiss during the warranty period if
the equipment is operated without observing the safety regulations. In any
such case, Carl Zeiss shall be exempt from statutory liability for accidents
resulting from such operation.
Exemption from warranty
Carl Zeiss shall be exempt from any warranty obligations should the user
fail to observe the safety regulations. Carl Zeiss only guarantees the safety,
reliability, and performance of the system if the following safety regulations
are closely observed.
The electrical installations of the room where the system is to be set up
must conform to IEC requirements.
Warning:
Any interruption of the PE conductor, either internally or externally, or
removal of the earthed conductor will make the system unsafe to use.
Any intentional interruption of the earthed conductor is illegal.
Attention:
To interrupt the power supply simply disconnect the data cable.
Setup, expansions, re-adjustments, alterations, and repairs must only
be carried out by persons who have been authorized by Carl Zeiss.
Use only those cables supplied by Carl Zeiss.
Do not allow any cables to trail across the floor, where they can be
snagged by people walking past.

Installation
Axiocam 105 color Installation + Reference Guide 3
General Notes
Please ensure the notes described below are adhered to when setting up
and operating the camera.
All connectors must be firmly and securely attached. Use the screw
coupling if possible.
Please protect the data cable from excessive heat (e.g. halogen lamps,
microscope fluorescence illumination).
Only use the camera in a clean and dry location.
The camera must be protected against mechanical impact. External
damage may affect the operation of inner components.
Keep chemicals and fluids away from the camera. To avoid the risk of
fire, do not use near inflammable liquids or gases.
Make sure there is sufficient ventilation of the camera head. Avoid
direct exposure to sunlight and locations near to heat sources
(radiators, stoves). Overheating can cause noisy images.
The camera housing can be cleaned using normal microscope cleaning
material.
In case of repair please contact your local Carl Zeiss representation.
Backup Copies
We strongly recommend that all users save the data they create, such as
images, measurement data, archives, reports, forms and documents, at
regular intervals on an external medium. Otherwise it cannot be excluded
that access to this data may be lost as a result of operational errors or
hardware defects. Carl Zeiss accepts no liability for consequential damage
resulting from insufficient data protection.

Installation
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1.2 System Requirements
The appropriate system requirements relating to hard- and software
equipment can be found in the file "Installation Guide.PDF" on the actual
product DVD.
Contents of Delivery
Axiocam 105 color
USB 3.0 data cable
PCI express (PCIe x1) Interface card
4 pin Molex power connector cable
15 pin SATA power connector cable
DVD-ROM with device driver and Installation+Reference Guide
Power connector cables, PCIe interface card, camera, USB 3.0 cable

Installation
Axiocam 105 color Installation + Reference Guide 5
1.3 Setting up the Camera System
Operating the Camera on a PC
Installing the PCIe bus interface card on the PC
Static electricity can damage electronic components. To protect
electronic components against static electricity, do not touch them
until you have grounded yourself to the casing of the device.
Never touch the contacts of the electronic components. We also
recommend that you work only on an antistatic mat.
For installation of the USB 3.0 PCIe interface card, please also
take into account the notes in the interface card documentation.
Only the basic principles of interface card installation are
described in this manual.
Molex power connector cable, PCIe interface card, USB 3.0 cable,
camera
Switch off your PC and all connected peripherals.
Disconnect the PC and the peripherals from the mains and open the
computer case.

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Examine your PC’s power supply to see if it has Molex type
connectors or SATA type connectors. Choose the appropriate power
connector cable for the following steps. If your PC’s power supply has
both connector types, choose only one cable.
Connect the appropriate power connector cable to one of the open
connectors from your computer’s power supply.
Connect the other end of the power connector cable to the PCIe
interface card.
Insert the PCIe interface card into the appropriate slot on your
computer.
Close the computer case and reconnect the peripherals.
Special details on opening your computer and on the slots it
contains can be found in the relevant documentation from your
PC’s manufacturer.
The provided power connector cables are not necessary if the
connectors from your PC’s power supply can extend far enough
to connect directly to the PCIe interface card. If it cannot extend
far enough and a power connector cable is needed as an
extension, use only one of the two provided cables.
Connecting the camera to the PC
Lay the data cable carefully between the camera and the PC.
Make sure that the cable is a safe distance from hot light
sources on the microscope, to prevent it being damaged by
heat.
Connect the supplied USB 3.0 cable to the Axiocam 105 color. Tighten
the screws with a screwdriver until it is finger tight.
Connect the other end of the USB 3.0 cable to the PCIe interface card.

Installation
Axiocam 105 color Installation + Reference Guide 7
Power is supplied to the Axiocam 105 color via the interface card. If you
switch off the PC, the camera is also switched off.
During operation the camera may heat up. This is quite safe.
Mounting the camera onto the microscope
To mount the camera onto your microscope’s TV port, use a C-mount
adapter.
Suitable adapters (example):
Port 44
Port 60
Port 60N
1.0x
452995 (44 C2/3")
456105 (60 C2/3")
426114 (60N C2/3")
0.63x
452997 (44 C2/3")
1069-414 (60 C2/3")
426113 (60N C2/3")
0.5x
452998 (44 C1/2”)
1069-415 (60 C1/2”)
426112 (60N C2/3")
The adapters are not supplied with the camera.
To mount the camera onto the microscope, remove the dust cap from the
camera’s C-mount port. Screw the adapter in as far as it will go. Then
mount the camera onto the microscope’s TV port. Please ensure that no
dust enters the opening of the camera or the microscope’s TV port.
Never touch the surface of the infrared barrier filter in front of the
CMOS sensor with your fingers. If you remove the camera from
the microscope, make sure you immediately cover the C-mount
opening with the dust cap provided, to prevent dust from getting
in.
Function Indicator
After you have switched on the PC, the LED on the camera will light up red.
As a rule, if the LED is red, this indicates that power is being properly
supplied to the Axiocam 105 color via the data cable. However the driver
has not yet been installed.

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For the definitions of the LED color signals, please refer to Notes on
Maintenance.
Checking the Equipment has been set up correctly
Check the following connections:
The Axiocam 105 color is mounted onto the microscope and is
receiving light.
The Axiocam 105 color is connected to the PC via the USB 3.0 data
cable.
If the LED on the Axiocam 105 color does not turn red now,
please check that you have carried out all of the above steps
correctly.
1.4 Installing the Driver Software
In order to operate the Axiocam 105 color the AxioVision software is
needed.
First connect the Axiocam 105 color to your PC (see the section "Setting up
the Camera System"), and then install the driver software.
Installing the Camera Driver using the Setup Program
Notes:
For installation under Microsoft® Windows 7, you need to log on with
administrator privileges. If you do not have access to administrator
rights on your computer, please speak to your system administrator.
In this section, installation is explained using screenshots from
Microsoft Windows 7.

Installation
Axiocam 105 color Installation + Reference Guide 9
If you have inserted a USB 3.0 PCIe interface card, which was
not supplied by Carl Zeiss or is supported by Carl Zeiss, into
your computer, you first need to install this card with the help of
the documentation enclosed by the manufacturer. Depending on
your operating system this installation may not be necessary, as
the drivers may already be integrated into the operating system.
After you have inserted the DVD into the PC’s DVD drive, installation will
start automatically.
If the automatic start function has been switched off on your system
(autorun) you must start the installation program manually in the following
way:
Double-click on the desktop icon Computer
Double-click on the desktop icon Computer
Start the Setup.exe or CheckOS.exe program.
After the start procedure, (automatically or manually) the installation
monitor will be displayed. Start the installation of AxioVision by clicking on
.
Select the language you want to install:

Installation
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Installation
Axiocam 105 color Installation + Reference Guide 11
Click OK and follow the installation wizard. There is no need to activate any
special feature during the software installation process.
To complete the camera installation, please go to our website to download
and run "Installation 01 - Axiocam 105 color":
http://www.zeiss.com/axiocam105
1.5 Notes on Maintenance
Camera Electronic
Camera, interface and power supply are maintenance free.
LED Indications
Red: Power supplied; no driver loaded, camera not detected
Green flash 3x: USB 3.0 connection detected
Green: Driver loaded; camera ready
Off: No power supplied
Optical System
The internal optical components of the camera should always be protected.
If no lens or TV adapter incorporating optics is screwed into the camera's
C-Mount thread, the camera's sensor and IR-filter must be protected by
screwing the protective cap onto the camera's C-Mount thread.
Cleaning the Infrared Barrier Filter or Protective Lens
Contamination of the infrared filter or the protective lens has an adverse
effect on the quality of the resulting image (dark points, cloudy structures in
the image). If there is dry dust on the front side of the infrared filter, you can
remove it with a soft brush or with cotton (wool) after unscrewing the lens
or TV adapter.

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Important Note on the Use of C-Mount Objectives
The camera can be mounted onto microscopes with standard TV-adapters
with C-mount connections. Carl Zeiss supplies the camera complete with a
pre-mounted infrared barrier filter, which offers the optical advantage for
use on microscopes of a lower sensitivity to dust.
The IR barrier filter is situated approx. 11 mm behind the outer edge of the
C-mount opening in the camera.
Due to this pre-mounted IR barrier filter, C-mount objectives that
screw more than 11 mm into the thread cannot be screwed into
the camera.