Zeiss Axiocam 702 mono User manual

User Guide
Axiocam 702 mono

2
Carl Zeiss Microscopy GmbH
Carl-Zeiss-Promenade 10
07745 Jena, Germany
[email protected]
www.zeiss.com/microscopy
Carl Zeiss Microscopy GmbH
Königsallee 9-21
37081 Göttingen
Germany
Effective from: 12 / 2015
© Jena 2015 by Carl Zeiss Microscopy GmbH - all rights reserved
This document or any part of it must not be translated, reproduced, or transmitted in any form or by any means, electronic or mechanical, including
photocopying, recording, or by any information or retrieval system. Violations will be prosecuted.
The use of general descriptive names, registered names, trademarks, etc. in this document does not imply, even in the absence of a specific
statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Software programs will
fully remain the property of ZEISS. No program, documentation, or subsequent upgrade thereof may be disclosed to any third party, unless prior
written consent of ZEISS has been procured to do so, nor may be copied or otherwise duplicated, even for the customer's internal needs, apart from
a single back-up copy for safety purposes.
ZEISS reserves the right to make modifications to this document without notice.
authorized dealer:
Pulch + Lorenz microscopy
Am Untergrün 23, -79232 March
tel: [phone]
fax: [phone]
mail: [email protected]
web: pulchlorenz.de

Content
User Guide Axiocam 702 mono|V1.0 en 3
1 About this guide 5
1.1 Introduction 5
1.2 Safety notes conventions 6
1.3 Text formats and conventions 6
2 Safety 8
2.1 Safety Notes 8
2.2 Limitation of liability 9
2.3 Warranty 9
3 Technical Data 10
3.1 Axiocam 702 mono 10
3.1.1 Analog signal amplification and typical quality parameters of the
image signal 12
3.1.2 High Dynamic Range Mode 12
3.1.3 Frame Rates 13
3.1.4 Spectral Sensitivity 13
4 Shipment 14
5 Connecting the camera 15
5.1 Camera Overview 15
5.2 Building in the interface card 15
5.3 Mounting the camera on the microscope 16
5.4 Connecting the camera to the PC 17
5.5 Connecting the trigger cable 18
5.6 Function indicator 20
6 Installing software and drivers 21
7 Trouble-shooting 22
7.1 Software 22
7.1.1 The camera does not appear in the menu of selectable cameras 22
7.1.2 You don't see a camera image on your screen 22
7.1.3 The color of my image does not correspond to the impression
through the ocular 23
7.2 Hardware 23
7.2.1 Vibrations 23

Content
4User Guide Axiocam 702 mono|V1.0 en
8 Maintenance 24
8.1 Optical System 24
8.2 Cleaning the infrared filter or protective glass 24
9 Disposal and Recycling 25

1 About this guide| 1.1 Introduction
User Guide Axiocam 702 mono|V1.0 en 5
1 About this guide
About this guide|
1.1 Introduction
Welcome Welcome to the Axiocam 702 mono user documentation.
The camera is a professional digital camera for universal light microscopy with a
high resolution 2,3 Megapixel sensor and a USB 3.0 interface.
To set up the camera correctly, follow the instructions in this guide step by step.
This monochrome camera offers a very broad range of spectral sensitivity. Due to
the very low noise level weakest signals can be detected. The sensor architecture
enables frame rates up to 1000 frames per second at short exposure times and
sensor sub frames. The broad dynamic range of the sensor resolves a very broad
intensity range in one single image. Due to these features, the camera is optimally
suited for live cell imaging applications.
Content Chapter Content
About this guide Includes an introduction and overview about this
guide.
Safety Provides important information on a safe handling
with the Axiocam 702 mono.
Read this chapter, before unpacking the
camera and putting it into operation.
Shipment Describes the contents of delivery and optional
attachments.
Technical data Includes all technical data to your camera.
Connecting the camera Provides detailed instructions on connecting and
using the camera.
Installing software and
drivers
Describes how to install the ZEISS software and
camera drivers.
Trouble-shooting Includes some solutions to various problems.
If you can not solve your problem, contact the
ZEISS support.
Maintenance Describes some measures for the maintenance and
care of your camera. For greater damage always
contact the ZEISS support.
Disposal and Recycling Includes important instructions for disposal and
recycling.

1 About this guide| 1.2 Safety notes conventions
6User Guide Axiocam 702 mono|V1.0 en
About this guide|
1.2 Safety notes conventions
The safety notes in this document follow a system of risk levels, that are defined as
follows:
CAUTION
Risk of personal injury
CAUTION indicates a potentially hazardous situation which, if not avoided, may
result in minor or moderate personal injury.
NOTICE
Risk of property damage
NOTICE indicates a property damage message. In addition, NOTICE is used for
data loss or corrupt data as well.
INFO
Indicates useful additional information. It helps you to make your daily work
easier, but they are all optional. There is no risk for personal injury or property
damage involved.
About this guide|

1 About this guide| 1.3 Text formats and conventions
User Guide Axiocam 702 mono|V1.0 en 7
1.3 Text formats and conventions
Bold texts
Bold is used for texts within the software like names of GUI elements (e.g. buttons,
sections, tools, menus), buttons on a device, product names, etc..
Font type "Courier"
Fonttype"Couriere"isusedforprogrammingcode.E.g.
macrocodeaswellasforanythingthatyouwouldtype
literallywhenprogramming,includingkeywords,data
types,constants,methodnames,variables,classnames,
andinterfacenames.
Shortcuts and key commands
Shortcuts do appear like Crtl+C, that means you must press Crtl-Key and C-Key
together.
Procedures
Following formats are used for procedures (instructive sequences):
Prerequisites ¢Stands for a condition which must be fulfilled before starting with the action.
Procedure 1Stands for a single step the user is asked to perform.
Web-Links
Web links do appear in blue text color. To open the linked website, simply right
click on the link. Just in case you are not connected to the internet, make sure you
have a internet connection established before opening the web link.

2 Safety| 2.1 Safety Notes
8User Guide Axiocam 702 mono|V1.0 en
2 Safety
Safety|
2.1 Safety Notes
The Axiocam 702 mono has been manufactured and tested by ZEISS according to
the regulations specified in CE and has left the manufacturer’s premises in perfect
working order. In order to ensure that this condition is maintained and to avoid any
risks when operating the system, the user must comply with any notes and
warnings contained in this manual. The manufacturer shall be exempt from
statutory liability for accidents should the operator fail to observe the safety
regulations.
CAUTION
Personal Injury
To avoid personal injury, read and adhere the safety notes below.
uTo avoid the risk of fire or explosion, do not use the camera near
inflammable liquids or gases.
uSetup, expansions, re-adjustments, alterations, and repairs must be carried
out only by persons who have been authorized by ZEISS.
uDo not allow any cables, particularly power cords, to trail across the floor,
where they can be snagged by people walking past.
uProtect the cables from excessive heat (e.g. halogen lamps, microscope
fluorescence illumination).

2 Safety| 2.2 Limitation of liability
User Guide Axiocam 702 mono|V1.0 en 9
NOTICE
To avoid equipment damage, data loss or corrupted data, read and adhere the
safety notes below.
uProtect the camera against mechanical impact. External damage may affect
the operation of inner components.
uKeep chemicals and fluids away from the camera.
uMake sure there is sufficient ventilation of the camera head. Avoid direct
exposure to sunlight and locations near heat sources (radiators, stoves).
Overheating can cause noisy images.
uUse the camera in a clean and dry location.
uAttach all connectors firmly and securely.
uUse only the accessories supplied by ZEISS, when applicable.
uUse only normal microscope cleaning material to clean the camera housing.
uContact your local ZEISS service organization, if a repair is necessary.
uSave all your data, such as images, measurement data, archives, reports,
forms and documents, at regular intervals on an external storage medium.
Otherwise it cannot be avoided that access to this data may be lost as a
result of operational errors or hardware defects. ZEISS accepts no liability for
consequential damage resulting from insufficient data protection.
Safety|
2.2 Limitation of liability
No warranty shall be assumed by ZEISS during the warranty period if the equipment
is operated without observing the safety regulations. In any such case, ZEISS shall
be exempt from statutory liability for accidents resulting from such operation.
Safety|
2.3 Warranty
ZEISS shall be exempt from any warranty obligations should the user fail to observe
the safety regulations. ZEISS only guarantees the safety, reliability, and performance
of the system if the safety notes are closely observed.

3 Technical Data| 3.1 Axiocam 702 mono
10 User Guide Axiocam 702 mono|V1.0 en
3 Technical Data
Technical Data|
3.1 Axiocam 702 mono
Feature Value
Sensor Model Sony IMX 174 , Exmor Pregius Global Shutter
Architecture, Active Pixel CMOS, Column ADC,
pre-selected sensor quality
Sensor Pixel Count 2.3 Megapixel: 1920 (H) × 1216 (V)
Pixel Size 5.86 μm x 5.86 μm
Sensor Size Image Diagonal 13,3 mm, equivalent to 1/1.2"
Sensor Format
11.3 mm (H) x 7.1 mm (V)
Spectral Sensitivity app. 350 nm-1000 nm, coated BK7 protective
glass plate,
Quantum Efficiency at 520 nm 72% (including
sensor cover slip)
Max Full Well Capacity
(typical)
32.000 e- with amplification 1x
Digitization Bit/Pixel 14 Bit / Pixel (12 Bit ADC, 14 Bit expansion
through data processing)
Pixel- Read Out Speed 594 MBit/s, parallel on 8 channels
Dynamic Range (typical) >5000:1 (single shot)
>25000:1 (HDR Mode)
Dark Current (typical) Typ. < 1.1 e-/p/s at 15 °C sensor temperature
Cooling Regulated Peltier-Cooling, (power supplied
through USB 3.0 and USB 2.0 ports),
Delta-T 20° C, sensor temperature 15°C
Dark Current Compensation Digital Dark Current Compensation for
optimum low light performance at long
exposure times,
automatic Hot Pixel Correction
Exposure Time Range 100 µs to 60 s
Live Image Repetition Rates ¢Max. frame rate >100 fps (depending on
the exposure time)
¢Resolution / Pixel 1920 x 1216

3 Technical Data| 3.1 Axiocam 702 mono
User Guide Axiocam 702 mono|V1.0 en 11
Feature Value
Data-Post Processing
(optional)
¢Objective specific shading correction
¢Sharpening, Noise Filter
¢Black Reference, Dark Current
Compensation
Special Features ¢Time Stamp from Camera for precise
acquisition time point
¢Adjustable intensity of status LED
Special Preset Modes Eight pre-loadable sets of imaging parameters
for speed-optimized multi-modal image
acquisition,
overlapping exposure and readout for fast
time lapse imaging
Binning Digital Binning from 1x1 up to 5x5 for
enhancing signal intensity and further
improvement of the signal-to-noise ratio
(Analog signal amplification is restricted in
Binning Mode to lower levels).
Region of Interest (ROI) User defined imaging sub area for
improvement of readout speed and reduction
of amount of data
Hardware Trigger Galvanic isolated I/O signals,
three output signals: exposure time, readout
time, trigger ready (i.e. for controlling external
mechanical shutters),
one trigger input for exposure control, 5V
auxiliary voltage, GND
Status LED Top LED: Camera Status (acquisition, power,
cooling, speed)
Back LED: Trigger status
Interface USB 3.0 SuperSpeed (5 Gbit/s), bandwidth
max. 300 Mbytes/s
USB 2.0 optional, with lower speed
Optical Interface C-Mount (17.5 mm)
Max. File Size per image app. 4.7 MB per image with 1920 x 1216
Pixels
Operating Systems Microsoft® Windows 7 Enterprise and higher

3 Technical Data| 3.1 Axiocam 702 mono
12 User Guide Axiocam 702 mono|V1.0 en
Feature Value
Size / Weight 10.8 cm x 7.8 cm x 4.3 cm / 500 g
Housing Blue anodized aluminum,
¼“ thread for camera equipment,
zero vibration by convection-cooling with
optimized cooling fins,
teflon coated C-mount thread, coated
protective glass plates
Certificates CE
Power Supply 7W power supply by USB 2.0 and USB 3.0-Bus
from PC
for maximum performance connection to USB
3.0 and USB 2.0 required, dual connection
cabling provided with camera
Ambient Conditions
(Operation)
+5° ... +35° Celsius
max. 80% relative humidity, non-condensing,
free air circulation required,
Ambient Conditions (Storage) -15° ... +60° Celsius
90% relative humidity at +40° Celsius,
80% relative at +20° Celsius, non-condensing
3.1.1 Analog signal amplification and typical quality parameters of
the image signal
Amplification Readout Noise Saturation
Charge
Dynamic
Range
1x 6,0 e- 32.000 e- > 5.000 : 1
2x 4,4 e- 16.000 e- 3.600 : 1
4x Opt. Gain 3,9 e- 8.000 e- 2.000 : 1
8x 3,79 e- 4.000 e- 1.000 : 1
16x 3,75 e- 2.000 e- 500 : 1
3.1.2 High Dynamic Range Mode
Optimized combination of two exposures based on photon shot noise theory,
automatic acquisition with special parameters:
¢0,2x exposure time at gain 1x
¢0,8x exposure time at gain 4x

3 Technical Data| 3.1 Axiocam 702 mono
User Guide Axiocam 702 mono|V1.0 en 13
Readout Noise Saturation Charge Dynamic Range
≙ 5 e- ≙ 160.000 e- > 25.000 : 1
3.1.3 Frame Rates
H x V Mode FPS@0.1ms
1920 x 1216 mono 128
1920 x 720 mono 210
1920 x 512 mono 288
1920 X 256 mono 534
1920 X 128 mono 881
1024 X 112 mono 1003
INFO
Exposure time and sensor sub area (ROI) influence the maximum frame rate.
3.1.4 Spectral Sensitivity
Fig.1: Axiocam 702 mono with Sony IMX 174 sensor (maximum QE reduced by
appr. 6% due to sensor cover slip)

4 Shipment
14 User Guide Axiocam 702 mono|V1.0 en
4 Shipment
Content Order Number
1 x Axiocam 702 mono camera 426560-9010-000
1 x USB cable (2 in one) for power supply (USB 2.0 =
black cable) and data transfer (USB 3.0 = blue cable)
1 x PCI express interface card ( 4 x USB 3.0)
1 x 15 Pin SATA power connector cable
1 x DVD with device driver for ZEISS software as well
as this guide as a PDF file.
Accessory (optional)
Fig.2: Axiocam trigger cable
Content Order Number
Trigger cable 426557-0001-000

5 Connecting the camera| 5.1 Camera Overview
User Guide Axiocam 702 mono|V1.0 en 15
5 Connecting the camera
Connecting the camera|
5.1 Camera Overview
Camera connections
Fig.3: Camera back
Number Description
1 Trigger connection
2 USB 2.0 connection for power supply and cooling
3 USB 3.0 connection for image / controller data and power
supply
4 ¼ “ photo thread (tripod connection)
Connecting the camera|
5.2 Building in the interface card
This topic describes how to build in the PCI express interface card (4 x USB 3.0) into
your PC.
If the interface card is already built in you can continue with chapter Mounting the
camera on the microscope [}16].

5 Connecting the camera| 5.2 Building in the interface card
16 User Guide Axiocam 702 mono|V1.0 en
NOTICE
Static Electricity
Static electricity can damage electronic components. To protect electronic
components against static electricity, do not touch them until you have
grounded yourself to the casing of the device. Never touch the contacts of the
electronic components. We also recommend that you work only on an antistatic
mat.
Prerequisites ¢You have read the documentation of the interface card and of your computer
before you build in the interface card.
Procedure 1Switch off your PC and all connected peripherals.
2Disconnect the PC and the peripherals from the mains and open the computer
case.
3Examine your PC’s power supply to see if it has Molex type connectors or SATA
type connectors. Choose the appropriate power connector cable for the
following steps. If your PC’s power supply has both connector types, choose
only one cable.
4Connect the appropriate power connector cable to one of the open
connectors from your computer’s power supply.
5Connect the other end of the power cable to the PCIe interface card.
6Insert the PCIe interface card into the appropriate slot on your computer.
7Close the computer case and reconnect the peripherals.
You can now mount the camera on the microscope and connect it to the PC via
the USB 3.0 interface, see Mounting the camera on the microscope [}16].
Connecting the camera|

5 Connecting the camera| 5.3 Mounting the camera on the microscope
User Guide Axiocam 702 mono|V1.0 en 17
5.3 Mounting the camera on the microscope
To mount the camera onto your microscope’s TV port, use a C-mount adapter. The
adapter is not included in the shipment. You will find some suitable examples for
adapters in the list below:
Port Name Order Number
44 Video Adapter 44 C 2/3" 1,0x 452995-0000-000
60 Camera Adapter 60 C 1" 1,0x 456105-9901-000
60 Video Adapter 60 C 2/3" 0,63x 000000-1069-414
60N Camera Adapter 60N-C 1" 1,0x 426114-0000-000
60N Camera Adapter 60N-C 2/3" 0,63x 426113-0000-000
Procedure 1Remove the dust cap from the camera’s C-mount port.
2Screw the adapter in as far as it will go.
3Mount the camera onto the microscope’s TV port. Ensure that no dust enters
the opening of the camera or the microscope’s TV port.
Connecting the camera|
5.4 Connecting the camera to the PC
Prerequisites ¢You have built in the interface card, see Building in the interface card [}15].
¢You have mounted the camera to the microscope, see Mounting the camera
on the microscope [}16]
Procedure 1Switch off the PC.
2Lay the USB cable carefully between the camera and the PC.
3Connect the USB 3.0 plug (blue color) to the USB 3.0 port on the interface
card.
4Connect the USB 2.0 plug (black color) to the USB 2.0 port on the interface
card.
5Connect the USB 3.0 plug to the USB 3.0 port on the camera.
6Connect the USB 2.0 plug to the USB 2.0 port on the camera.
7Switch on the PC.
If you switch on the PC, the camera will be also switched on.
Power is supplied via the USB 3.0 cable. The USB 2.0 cable supplies the peletier
cooling. By switching off the PC, the camera will be switched of as well. If the
camera is assembled correctly, the status LED will light red. If you start the software
the LED will turn blue. You`ll find a detailed description of the LED status light in the
chapter Function indicator [}20]

5 Connecting the camera| 5.5 Connecting the trigger cable
18 User Guide Axiocam 702 mono|V1.0 en
Connecting the camera|
5.5 Connecting the trigger cable
In this chapter you will learn how to connect the trigger cable (optional) to the
camera and the SVB (signal distribution box).
Fig.4: Trigger cable

5 Connecting the camera| 5.5 Connecting the trigger cable
User Guide Axiocam 702 mono|V1.0 en 19
Number Description
1 Trigger (Trigger-In)
2 GPO 0 (Trigger Out, for exposure time incl. predelay,
coresponding to shutter control
3 GPO 1 (Trigger Ready, for Readout Time data)
4 GPO 2
5 GPOPWR
6 AUXPWR
7 Commercial Micro-D
Procedure 1Plug the connector Commercial Micro D (7) in the trigger connection on the
back of the camera, see Camera Overview [}15].
2Plug the connector Trigger (1) in the socket Trigger Out of the SVB (Signal
distribution box).
Fig.5: Signal distribution box (SVB) backside
3Plug the connector GPO 0 (2) in the socket Trigger In of the SVB (Signal
distribution box).
4Connect the connectors GPOPWR (5) and AUXPWR (6) of the trigger cable
together.

5 Connecting the camera| 5.6 Function indicator
20 User Guide Axiocam 702 mono|V1.0 en
Connecting the camera|
5.6 Function indicator
Signal color Description
Blue USB 3.0 connection (Camera) connected with USB 3.0
connection (PC)
Power supply (USB 2.0 cable) attached
Recommended configuration for best camera performance
Green USB 3.0 connection (Camera) connected with USB 2.0
connection (PC)
Power supply (USB 2.0 cable) attached
Violet USB 3.0 connection (Camera) connected with USB 3.0
connection (PC)
Power supply (USB 2.0 cable) not attached
Yellow USB 3.0 connection (Camera) connected with USB 2.0
connection (PC)
Power supply (USB 2.0 cable) not attached
Red Power supply attached, no driver loaded, camera not yet
initialized
No light No power supply or software shut down (camera in power
down mode)