Abaxis VetScan HM5 User manual

QUICK REFERENCE GUIDE
Easy operation — fast results
Closer to Care.

• Unpack the VetScan HM5 analyzer from its shipping container and put out the
accesory box.
Note: Store the shipping box, accessory box, and packaging
materials in case you need to use them later.
• Place the analyzer on a level surface.
• Use the latch to open the side door on the unit, as shown.
• Inspect the unit for any visible signs of damage.
• Remove foam 1 (and tape2) as shown below.
Unpacking and Setup
1
2

< 45 cm not preferred
Up to 45 cm
below is ok
Ideal
•Open the accessory box and use this list to make sure you received all
components of the Vet-Scan HM5 system.
• VetScan HM5 Analyzer.
• VetScan HM5 Operator’s Manual.
• External power supply.
•Mini-keyboard
• Four sample tube adaptors.
• Reagent Tubing Kit.
Selecting a Location
The analyzer must be installed in a suitable location.
• The location should be flat, level, sturdy, vibration-free, and as dust-free as possible.
• Leave at least 50 cm of clearance on all sides of the instrument to allow for
adequate airflow and access to connectors.
• Maintain a minimum of 20 cm behind the analyzer’s rear panel for heat dissipation
and tube clearance.
• Leave enough space for the reagent pack.
Connecting the Reagent Pack
• Place the reagent pack near the analyzer, either beside
or behind the instrument.
• For best results, place the reagent pack at the same level as
the analyzer. If you have to place the reagent pack on a lower
level, make sure that it is no more than 45 cm below the
level of the analyzer’s reagent inlets, as measured from the
bottom of the reagent pack to the bottom of the analyzer.
Note: Do not place the reagent pack on a higher level.
Closer to Care.

Connect the reagent pack as follows.
• Open the plastic bag located in the accessory box, and take out the six
color-coded reagent tubes and caps with drop-down tubes.
• Attach the supplied connector
tubing kit to the instrument,
as shown.
• Open the reagent pack box. Open each reagent container (lyse, lyse 2, rinse, cleaner,
and diluent) by unscrewing the container caps and piercing the bottle top seals.
• Re-cap each container using the bottle cap with the appropriate color-coded connec-
tor and drop-down tube.
•Flip diluent canister 180 degrees prevent reagent errors by ensuring that tubes are
not kinked when the box lid is closed.
•Connect each color tube to its matching color outlet. The connectors should
snap into each other when properly connected.
• Connect waste bottle.
Initialization of the HM5
• Connect Power Supply.
• Install paper into the built in printer.
• Turn on the analyzer by using the power switch on the upper le of its rear panel.
•The analyzer then displays several screens while it runs its soware start-up procedure.
•Touch Measure, then touch Run.
•When prompted, touch OK to run a blank. ( Also see “Running a blank”).
•The analyzer then initializes its pneumatic system and runs a blank measurement.
Attach the 2” tubing to the
stainless steel hose barbs
Attach the male
connector to the
reagent tubing
Snap the male connector into the
female connector
Attach the 2” tubing to the
stainless steel hose barbs
Attach the male
connector to the
reagent tubing
Snap the male connector into the
female connector
• Insert the six male connectors into
the free ends of the color-coded
reagent tubes.

Test: analyze samples and
run blanks
HOMESCREEN
Database: search, retrieve, view
and manage up to 5,000 records
Settings: manage printer,
testing, export and date/time
and other analyzer settings
Printer: Manage printer settings
and printout options
Maintenance: clean, calibrate,
run quality controls and manage
reagent status
Exit: select shutdown options and
manage user accounts
General Settings: select
analyzer options and language
Date and Time: change date
and time options
Measurement: select units, set
normal ranges for species and
data export options
Touchscreen Icons (list of icons and their meanings)
CBC analysis menu
stores all results
from test menu
adjust settings for
analyzer controls,
printing, and data export
maintenance,
cleaning, QC
and calibration
shutdown and
logout options
Closer to Care.

• Touch the Measure icon.
• Enter the Patient ID on the “New Sample Screen”.
•Select animal Type.
•Mix the sample well by slowly inverting the tube 10-15 times. Insert the
well-mixed EDTA preserved whole blood sample into the appropriate tube
adapter, on the sample rotor, and remove the cap.
• Touch the Run button.
If your HM5 is set for autoprint, your results will be
printed automatically.
If not, press the Print button to print results
Test Results (and blank cycles) are automatically saved
to the HM5 database.
They can be viewed by pressing the database function key
Running a Sample

Analysis is complete in less than four minutes. All results and histograms
are automatically saved in the analyzer’s database.
• When analysis is complete, the analyzer displays all measured and
calculated parameters, as well as the WBC, EOS, RBC, and PLT
histograms:
If the analyzer is not set to print results automatically, press Print to
print the results.
Results falling outside of the specified species reference range are
marked as follows:
• Results above the reference range are flagged and marked with a
plus sign (+).
• Results below the reference range are flagged and marked with a
minus sign (–).
• Warning and error flags are marked with upper- and lower-case letters
at the bottom.
Interpreting Results
Closer to Care.

Running a Blank
A blank must be run every 12 hours. Each blank measurement remains
valid for 12 hours of continuous operation, aer which the analyzer displays
BLANK needed and a new blank is required. In addition, a new blank must
be run each time the analyzer has been powered on and off again.
• If the analyzer is in standby mode, touch the measure icon.
• Touch the Run button.
• If a blank is necessary, the “Blank needed” screen appears.
• Touch OK to confirm and run the blank.
• Check the results to see whether the blank falls within the acceptable
ranges shown in the following table. If so, press Accept.
Acceptable blanks will have
the following readouts:
WBC 0.0-0.5
RBC 0.0-0.05
PLT 0-26
HGB 0-10
.
2

Running Controls
Controls are run to assess if the analyzer is maintaining calibration.
• Touch the Maintenance icon.
• Touch the Quality control button.
• Touch the QC level (e.g. QC Normal) that will
be used.
• Touch References to load the assay and gap values
• Enter QC values (choose one of three methods):
a. Enter manually - values can be entered by
hand by touching the appropriate fields.
b. Enter by USB pen drive - the QC values file
can be loaded by inserting the USB drive into
a USB port and touching Load.
c. Enter by Barcode reader - press Load
Datamatrix on the Quality Control screen.
Scan the 2D barcode on the QC package insert.
• View table of QC databases.
• View QC diagram (for a Levy-Jennings graphic
representation of the QC history).
• If the QC results were within range, proceed
to running samples. If the QC was out of
range, repeat QC run. If the QC is repeatedly
out of range, proceed to calibration.
(see Calibrating the Analyzer).
Closer to Care.

Changing the Reagent Pack
• In the Maintenance menu, select Reagent status.
Select Replace and then Change pack.
• Select Prime All to prime the reagent tubing.

Calibrating the Analyzer
Calibration is rarely necessary on the HM5, but should be performed when
the controls are running out of range.
• Warm up HM5 Normal Control to room temperature. Do not use other
level controls for calibration.
• Touch Maintenance and select Calibration.
• Touch Measure. Set Sampling Depth to default
(--). Press Accept.
• Enter the lot number and assay values from the
calibrator package insert and press Accept.
• Mix the Normal Control well by inverting it ten
times, uncap the vial and place it into the
vial adaptor.
• Touch the Run button. When the results display
the screen will show the run as CAL3/1.
• Mix the control well and repeat step
6 twice more.
• Aer three runs, press the Evaluate
button and the screen will display the
calibration factors. If there are no
errors and the Factors are equal to
or between 0.8 and 1.2, press Accept.
Consult technical support if there are any errors.
Closer to Care.

Recommended Maintenance
The VetScan HM5 requires minimal maintenance, but
does need attention to proper sample handling and
cleanliness to ensure optimal results.
Daily
Run a blank.
Weekly
Wipe down the HM5 wash head with a damp, lintfree cloth.
With each change of reagent pack
Perform Vetscan HM5 bleach cleaning procedure.
Enzymatic Cleaning
An enzymatic cleaning is recommended aer
approximately every 100 tests or at the time of a
reagent pack change. Touch Maintenance,
Cleaning, then Enzymatic cleaning. Place an EDTA
tube with approximately 300 μl of Vetscan
enzymatic cleaner concentrate into the sample
holder and touch OK.
wash head

Shutting Down the Analyzer
Proper shut downs rinse the analyzer to keep it in
good condition. So it is important to perform shut
downs when the analyzer will not be used for
extended periods of time. It is preferable to leave
the analyzer on overnight if samples will be run
within a couple of days.
Between 3-10 days of non-use
If the analyzer will not be used for more than 3 days but
less than 10 days, follow this procedure.
1. Touch the Exit icon.
2. Select shut down, then press accept.
3. Power off the analyzer when it emits a tone.
More than 10 days
Follow the instructions below if the analyzer will be
shipped or not used for more than 10 days. Detailed
instructions will be displayed on the screen.
1. Press the Exit icon.
2. Select prepare for shipment.
3. Follow on screen instructions and disconnect
the reagent tubing lines and connect the
cleaning tube.
4. Put the end of the cleaning tube into distilled water.
5. Power off the analyzer when instructed.
Closer to Care.

Results Flags (Partial list)
Capital letters indicate WBC related flags; lower-case letters indicate RBC
or PLT-related flags. For more details, consult your HM5 operator’s manual.
Flag Meaning Solution
EEmpty sample Lower needle height
M,N Linearity error (WBC)
- cell concentration
too high
Prime lyse and repeat blank. View
manual smear to confirm high WBC
C,Q WBC clogging Run 3-5 self cleanings and enzymatic
cleaning
LPotential lyse resistant
RBCs in canine samples
Re-run sample at higher
lyse volume
XEOX blank high Check that Lyse2 is room tempera-
ture
Y EOS clogging Run 3 automated self cleanings and
repeat analysis
ZNoise in EOS measurement Check Lyse2 volume and
prime Lyse2 if needed
bRBC blank is high Clean wash head; run self cleaning;
bleach clean analyzer/tubes; change
reagents if necessary
cRBC/PLT clogging Ensure that reagents are room
temperature run self cleanings and
enzymatic cleanings
pPLT blank is high Clean wash head; repeat blank 2-3x;
run 5 cycle cleaning; run enzymatic
cleaning; bleach clean analyzer/tub-
ing; change reagent pack

Notes
Closer to Care.

790-7011E-ENG Rev.A
Closer to Care.
ABAXIS Europe GmbH
Bunsenstr. 9-11
64347 Griesheim · Germany
E-mail [email protected]
www.abaxis.de
ABAXIS Inc.
3240 Whipple Road
Union City, CA 94587 · USA
Phone [phone]
www.abaxis.com