Abaxis VetScan SA User manual

USER'S MANUAL v1.1 SOFTWARE
FOR VETERINARY USE ONLY

VETSCAN SA USER’S MANUAL v1.1 SOFTWARE | 2
Abaxis, Inc. 3240 Whipple Road, Union City, CA 94587 USA | [phone] | www.abaxis.com
ABAXIS Europe GmbH, Bunsenstr. 9-11, 64347 Griesheim, Germany | [phone]
FOR VETERINARY USE ONLY
Abaxis and VETSCAN are registered trademarks of Abaxis, Inc. Reproduction in any form, either in print or electronic, is
prohibited without written permission of Abaxis, Inc. Copyright ©Abaxis, Inc. 2018. 1550-7013 Rev. B

VETSCAN SA USER’S MANUAL v1.1 SOFTWARE | 3
Disclaimer
For Veterinary Use Only.
No part of this publication may be reproduced or transmitted in any form or by any means,
electronic or mechanical, including photocopy, recording, or any information storage or retrieval
system, without permission in writing from Abaxis, Inc.
Abaxis reserves the right to modify its products and services at any time. This user’s manual
is subject to change without notice. Although prepared to ensure accuracy, Abaxis assumes
no liability for errors, or for any damages resulting from application or use of this information.
No rights are granted for diagnostic uses. No rights are granted for use of the product for
commercial application of any kind, including but not limited to manufacturing, quality control,
or commercial services, such as contract services or fee for services. It is the responsibility of the
purchase/end user to acquire any additional intellectual property rights that may be required.
Abaxis warrants the VETSCAN Sediment Analyzer System against defects in materials and
workmanship for 1 year. Contact your Abaxis representative for the details of the warranty. If
any defects occur in the analyzer during this warranty period, Abaxis will repair or replace the
defective parts free of charge. The following defects, however, are specifically excluded:
1. Defects caused by improper operation.
2. Repair or modification done by anyone other than Abaxis or an authorized agent.
3. Use of fittings or other spare parts supplied by anyone other than Abaxis.
4. Damage caused by accident or misuse.
5. Damage caused by disaster.
6. Corrosion due to use of improper solvent or sample.
The device’s protection will be impaired if used in a manner not specified by the manufacturer.

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Declaration Of Conformity
Manufacturer:
Abaxis, Incorporated
3240 Whipple Road
Union City, CA 94587, USA
European Authorized Representative:
Achim Henkel
ABAXIS Europe, GmbH
Bunsenstr. 9-11
64347 Griesheim, Germany
The Directives covered by this Declaration
EU Machinery Directive 2006/42/EC
EU RoHS2 Directive 2011/65/EU
The Products Covered by this Declaration
VETSCAN SA sediment analyzer
The Basis on which Conformity is being declared
The manufacturer hereby declares under his sole responsibility that the products identified
above comply with the essential health and safety requirements of the Machinery directive
2006/42/EC.
The manufacturer has applied the following standards:
EN ISO 13485:2012 Medical devices-Quality Management systems- Requirements for
regulatory purposes
EN 61326-1 Electrical Equipment for Measurement, Control and Laboratory Use –
EMC Requirements – Part 1: General Requirements
IEC/EN 61010-1 Safety Requirements for Electrical Equipment for Measurement, Control
and Laboratory Use Part 1: General Requirements
IEC/EN 61010-2-081 Particular Requirements for Automatic and Semi-Automatic Laboratory
Equipment for Analysis and Other Purposes
The technical documentation required to demonstrate that the products meet the requirements
of the Machinery directive 2006/42/EC can be made available to relevant authorities within a
reasonable period of time.

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Table of Contents
Introduction ..................................................................................................................................................... 6
Intended Use ................................................................................................................................................... 7
Result Classifications ........................................................................................................................... 8
Result Warnings .................................................................................................................................... 8
System Overview .......................................................................................................................................... 9
Safety ................................................................................................................................................................. 12
VETSCAN SA Menu ...................................................................................................................................... 13
Running a Sample ......................................................................................................................................... 15
Sample Collection ................................................................................................................................ 15
Sample Handling .................................................................................................................................. 15
Running a Test ....................................................................................................................................... 16
SA+UA Test ............................................................................................................................................. 17
SA Only Test ........................................................................................................................................... 19
UA Only Test ........................................................................................................................................... 20
Urinalysis Results ........................................................................................................................................... 22
SA Images Panel ................................................................................................................................... 23
SA Results Panel .................................................................................................................................. 24
UA Results Panel ................................................................................................................................... 24
Urinalysis Printout ................................................................................................................................ 25
Retest SA .......................................................................................................................................................... 26
Database – Reviewing Previous Results ............................................................................................... 28
Combining SA and UA Results ........................................................................................................ 29
Maintenance .................................................................................................................................................... 30
Maintenance Schedule ........................................................................................................................ 31
Cleaner Reagent Cycle ....................................................................................................................... 32
Kit Change ............................................................................................................................................... 33
Empty Waste Container ..................................................................................................................... 33
Clean Fan Filter ...................................................................................................................................... 34
Software Updates .......................................................................................................................................... 35
Settings ............................................................................................................................................................. 36
Appendix A: Installation Overview ......................................................................................................... 37
Appendix B: Printer Installation ............................................................................................................... 38
Appendix C: UA Installation ...................................................................................................................... 39
Appendix D: VETSCAN SA Analyzer Specifications ........................................................................ 40
Appendix E: Symbols ................................................................................................................................... 41
Appendix F: Safety and Regulatory Compliance .............................................................................. 43
Appendix G: Part Numbers ........................................................................................................................ 44
Appendix H: Troubleshooting ................................................................................................................... 45
Appendix I: Gross Examination, Specific Gravity, and Dilution Fields ............................................. 50
Appendix J: Understanding Urine Dilutions on the VETSCAN SA.............................................. 51
Appendix K: VETSCAN SA Suggested Normal Ranges .................................................................... 52
Appendix L: VETSCAN UA Results Bins ................................................................................................ 53

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Introduction
The VETSCAN SA enables practitioners to perform urine microscopy with consistency, efficiency,
reliability and accuracy at the point-of-care. Its high-resolution urine sediment images and
classification capabilities improve laboratory workflow, efficiency, and precision.
When paired with the VETSCAN Urine Analyzer (UA), the VETSCAN SA+UA system provides a
comprehensive urinalysis solution.

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Intended Use
The VETSCAN SA is intended for veterinary use only for testing of urine sediment in mammalian
urine samples, particularly dogs and cats. Urine samples collected from other species may be
run on the analyzer, but have not been validated. The VETSCAN SA will provide quantitative and
semi-quantitative analysis of the following sediment particles in urine samples:
Blood Cells
1. Red Blood Cells
2. White Blood Cells
Epithelial Cells
3. Squamous Epithelial Cells
4. Non-Squamous Epithelial Cells
Casts
5. Hyaline Casts
6. Non Hyaline Casts
Bacteria
7. Bacteria Rods
8. Bacteria Cocci
Crystals
9. Struvite Crystals
10. Calcium Oxalate Crystals
11. Unclassified Crystals
Urine testing on the VETSCAN SA is intended to help detect renal and urinary tract
abnormalities. It is designed to be utilized in combination with urine chemistry examination and
other clinical diagnostics to obtain a complete evaluation of the disease process affecting the
patient.

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Result Classifications
The VETSCAN SA reports both quantitative and semi-quantitative results.
Quantitative Results
Parameter SA Display ≤ 5.0 Range Above Range Units
Red Blood Cells RBC NTR 5-500 >500 /µL
White Blood Cells WBC NTR 5-500 >500 /µL
Parameter SA Display ≤ 1.0 Range Above Range Units
Squamous Epithelial Cells Sq Epithelial NTR 1-50 >50 /µL
Non-Squamous Epithelial Cells Non Sq Epithelial NTR 1-50 >50 /µL
Hyaline Casts Hyaline NTR 1-5 >5 /µL
Non Hyaline Casts Non Hyaline NTR 1-5 >5 /µL
Semi-quantitative Results
Parameter SA Display Semi-quantitative Results
Bacteria Rods Bacteria-Rods NTR + ++ +++ ++++
Bacteria Cocci Bacteria-Cocci NTR + ++ +++ ++++
Struvite Crystals Struvite NTR + ++ +++ ++++
Calcium Oxalate Crystals CaOx dihydrate NTR + ++ +++ ++++
Unclassified Crystals Crystals-other NTR + ++ +++ ++++
NTR = None To Rare
Result Bin Definition
+Few
++ Moderate
+++ Many
++++ Numerous
Result Warnings
The VETSCAN SA also displays warnings above the test results in a section titled SA Test Results:
SA Test Results display text: Description Action
Completed Nothing abnormal or
questionable None
Review images SA detected abnormal levels
of undefined sediment.
Look through SA images; review
on manual slide and/or confirm
with send-out laboratory
TNTC – Dilute and retest
Too Numerous To Count;
too many particles in image,
sample dilution required
Dilute sample and re-run
Possible short sample
SA may not have received
enough sample to complete
test
Re-run test and ensure sample
probe touches tube bottom
Not measured UA only test selected None
The SA Test Results section will turn orange if “Review images”, “TNTC – Dilute and retest”, or
“Possible short sample” are indicated.

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System Overview
The VETSCAN SA Sediment Analyzer Final Assembly includes:
• VETSCAN SA Sediment Analyzer (PN 1550-1000)
• VETSCAN SA Tubing Kit (PN 1550-1001)
• VETSCAN SA Waste Bottle (PN 1550-1002)
• VETSCAN SA USA Power Cord (PN 1550-1005)
Optional accessories to be ordered separately (not included in the system):
• VETSCAN SA Keyboard (PN 1550-1003)
• VETSCAN SA Mouse (PN 1550-1004)
• VETSCAN SA Display Monitor (PN 1550-1007)
• VETSCAN SA International Power Cords (see Appendix G for listings)
The VETSCAN SA analyzer works with the following reagent, cleaner and control:
• VETSCAN SA Reagent Kit (PN 1550-9000)
• VETSCAN SA Cleaner Kit (PN 1550-9001)
• VETSCAN SA Control Kit (PN 1550-9002)

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Front Panel
1. Sample probe
2. Blue start paddle
Rear Panel
1. Input/output panel
2. Power connection
3. Reagent connection panel
Side Panels
1. Door latch
2. Power switch
3. Fan filter door
WASTESENSOR
1
2
1
2
1
2
3
3
RINSE A
RINSE B

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Ports
(1) Ethernet (2) USB ports (3) VGA monitor port
(4) Mouse port (5) Keyboard port
WASTESENSOR
1
4
Input/output panel Reagent connection panel
2
3
5
RINSE A
RINSE B
Note: The Ethernet ports have not been enabled yet but will be in a future software update.
The HDMI and DVI ports are not supported and should not be used to connect a display monitor.

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Safety
The VETSCAN SA system is designed for maximum user safety and for indoor use only.
The plastic enclosures are made of thick injection-molded acrylic to create a leak-free test
environment. The safety metal cover surrounds the system to protect the user from exposure to
electrical current.
Before use, inspect the VETSCAN SA system for cracks and chips, which may allow the test
sample to leak from the tubes and cause a potential electrical hazard. Additionally, inspect all
electrical cables and plugs for loose connections, cracks, breaks or corrosion. Do not use any part
that is cracked, charred, or corroded. These parts may also cause a potential electrical hazard.
If leaking sample is detected, disconnect the power to the analyzer immediately.
Power to VETSCAN SA system is supplied by an external DC voltage power supply. All Abaxis’s
power supplies have met the safety requirements. Abaxis supplies the recommended power
cords for this unit. Only use Abaxis supplied power supplies specified for the VETSCAN SA
system. Refer to Appendix for listings of power cords for your region.
Important: The VETSCAN SA analyzer is certified to meet IEC 61010-1 safety standards. IEC-
certified products are safe to use when operated in accordance with the instruction manual. This
analyzer should not be modified in any way. Alteration of this analyzer will:
- Void the manufacturer’s warranty
- Void IEC 61010-1 safety certification
- Create a potential safety hazard
Contact your local Abaxis representative before using a part that may be considered hazardous
or if leaking is detected.
When moving the SA analyzer, 2 people should handle any lifting.
Proper use of gloves is recommended when testing. OSHA requirements for personal protective
Equipment (PPE) are set forth in the code of Federal Regulations (CFR) at 29 CFR 1910.132
(General Requirements); 29 CFR 1910.138 (Hand protection); 29 CFR 1926.95 (Criteria for
standard personal protective equipment). Any gloves with impaired protective ability should be
discarded and replaced. Consider the toxicity of the chemicals and factors such as duration of
exposure, storage, and temperature when deciding to reuse chemically exposed gloves. Please
also consider local Occupational Safety and Health regulations.

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VETSCAN SA Menu
When the VETSCAN SA powers on, the top menu has three selections:
Measure is for running samples.
Database is for reviewing previous tests run.
Tools is for changing settings and maintenance of the analyzer.

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The Measure menu screen is divided into five different panels.
Measure Menu Screen
1. Left Side Panel: guides the user through running a test using images and text.
2. SA Images Panel: images captured at end of sample analysis.
3. Sample Entry Panel: where a user enters in all the inputs related to the sample.
4. SA Results Panel: results classification of particles from the images captured.
5. UA Results Panel: results received from the UA analyzer.
2 4
3 5
1

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Running a Sample
Sample Collection
Urine samples may be collected by any of these methods:
1. Cystocentesis: Using an ultrasound to visualize the bladder, a small needle can be imaged
into the bladder through the skin and draw urine sterilely into the syringe for sampling and
culture, if needed.
2. Catheter: Sterilely place a urinary catheter within the urethra and advance it into the
bladder. Collect the sample using a sterile syringe. Sample may be used for testing and
culture if needed.
3. Free catch/voided (mid-stream): Using a sterile urine collection jar, collect a mid-stream
urine sample. This is not sterile due to contamination from the urethra and sexual organs.
Culture is not recommended for this type of sample.
Although free catch samples can be utilized, it is strongly recommended images from free
catch samples be reviewed and if there is a great deal of bacteria or amorphous debris, a sterile
sample should be obtained and re-run on the SA to verify the changes noted are physiological.
It is recommended to acquire at least 1 mL of urine sample prior to running a test. The SA
requires approximately 650 µL (0.65 mL) of sample to run a SA test and additional sample is
required for application to UA strip if also running a UA test.
Sample Handling
Do not centrifuge urine samples before use on the VETSCAN SA and VETSCAN UA. Use fresh,
well mixed urine.
Urine samples may be tested immediately after collection up to one hour post collection, if
stored at room temperature.
If urine samples are not tested within one hour of collection, samples may be refrigerated
(36-46 °F, 2-8 °C) for up to 4 hours.
Cold samples must be warmed to room temperature prior to testing.
Dilute the sample if necessary. A dilution should be considered if the sample is grossly examined
and found to have color suggestive of hematuria (red, dark red, brown) and/or decreased clarity
or high turbidity (very cloudy, opaque).
For a dilution decision chart, see Appendix J. Dilute the sample with 0.9% normal saline. For
most samples, a 1:8 dilution is recommended (0.1 mL urine, 0.7 mL saline).
SPECIAL CASES: In SA Software v1.1, if a UA10 or UA14 strip is utilized and run with the SA
for the same patient, pH is factored into the crystal classification to more accurately identify
calcium oxalate crystals and struvites. If a patient with stones or severe crystalluria is currently
on a prescription diet that alters the urine pH to decrease crystal formation or dissolve a urinary
stone, update to the SA Software v1.1 crystal identification that factors in the urine pH should
be disabled for more accurate results. The pH in this case may cause crystals to be misclassified
due to the patient’s clinical status. To disable the pH aspect of the classification, the SA must
be run separately from the UA then combined later in the Database. See Database – Reviewing
Previous Results section for detailed information on this process.

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Running a Test
The VETSCAN SA and UA analyzers can be connected together to produce a full urinalysis
report. The SA analyzer becomes the urinalysis workstation where all data entry will take place
and sample runs are initiated.
Follow on-screen instructions. Patient ID, Species and Test Type are minimum required selections
before the Run button is enabled. These required inputs are locked at the time the Run button
is clicked and may not be changed during and after the measurement. All other inputs are
optional and may be edited or entered after the test has been completed. Once a test begins,
the measurement cannot be canceled.
There are three test workflows that can be initiated on the VETSCAN SA, two of which require
a VETSCAN UA connected to the SA. Since there are two strip types (UA10 and UA14) on the
VETSCAN UA, five unique Test Types can be selected.
Analyzers used
Test Workflow (3) SA+UA test SA only test UA only test
Test Type (5) SA+UA10, SA+UA14 SA UA10, UA14
See Appendix I for details on Gross Examination, Specific Gravity, and Dilution fields.
If a SA+UA test is selected, always start the SA test first. Each of the three test workflows are
detailed below.

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SA+UA Test
SA Test
1. Enter Patient Information:
Enter Patient ID and select Species (Cat, Dog, Other, Control).
Enter Patient Name and Doctor Name if desired.
2. Enter physical characteristics of the sample (optional):
Use the drop-down option lists to select gross examination fields for the sample (Collection
Method, Color, and Clarity) for the medical record.
3. Enter specific gravity of the sample (optional):
Input specific gravity value of the sample, if available, in format 1.0xx. Specific gravity is often
measured using a refractometer.
4. Select Test Type (2 options): SA+UA14, SA+UA10
5. Select Dilution if necessary:
Dilute the sample and select correct dilution. Undiluted is the default setting and appropriate
for most samples. Diluted samples are to be used on the SA analyzer only. If diluting a sample,
reserve undiluted sample for UA testing.
6. Click Run.
7. Mix urine sample by gently inverting.
8. Place tube below sample probe and press blue start paddle.
1. Hold the sample below the sample probe leaving
a gap to the probe tip to allow the sample probe to
aspirate air prior to moving down for sampling.
2. Ensure sample probe touches tube bottom. and
maintains contact until the probe starts to move
back up.
UA Test
9. Re-mix urine sample by gently inverting.
10. Confirm Strip Type, Species, and PID (Patient ID) on UA analyzer. Prepare to apply urine with
dropper on UA strip. If incorrect, the UA test can be canceled by pressing the UA Back button, and
the overall test will be changed to SA only.
11. Press Test (Test Tube icon) button to start test. Countdown
timer appears. Perform the next three steps before the
timer reaches 35.

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12. Apply urine sample to strip. Use a dropper pipette to
drop urine over entire strip, completely wetting each
pad. Alternatively, strip may be dipped into urine
sample, completely wetting every pad.
13. Blot either long edge of strip on absorbent paper to
remove excess urine. DO NOT blot the top surface
of the pads to avoid contamination. Work quickly to
blot excess urine.
14. Place strip on the UA Strip Tray. The end of the strip
should align with the end of the trough in the Strip Tray,
approximately where the top cover of the UA analyzer
is indented. This step MUST be completed before the
countdown timer reaches 35 as the Strip Tray will begin
to slide into the analyzer. The UA analyzer will time the
reactions and scan when appropriate.
WARNING! If the Strip Tray has already moved into the analyzer to start scanning before the
strip is placed in the tray, an error will be reported on the UA and sent to the SA. The test will be
changed to a SA only test.
While measuring, the SA screen is locked, and navigation to other screens is prohibited. Also,
both the SA status indicator light (top right of the SA Results Panel) and the UA status indicator
light (top right of the UA Results Panel) will display yellow during measurement process. Do not
disconnect UA from SA during testing process. If the UA becomes disconnected during testing,
the UA test will be canceled automatically on the SA, and complete as an SA only test.
UA Test (continued)

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SA Only Test
1. Enter Patient Information:
Enter Patient ID and select Species (Cat, Dog, Other, Control).
Enter Patient Name and Doctor Name if desired.
2. Enter physical characteristics of the sample (optional):
Use the drop-down option lists to select gross examination fields for the sample (Collection
Method, Color, and Clarity) for the medical record.
3. Enter specific gravity of the sample (optional):
Input specific gravity value of the sample, if applicable, in format 1.0xx. Specific gravity is often
measured using a refractometer.
4. Select Test Type: SA
5. Select Dilution if necessary:
Dilute the sample and select correct dilution. Undiluted is the default setting and appropriate for
most samples.
6. Click Run.
7. Mix urine sample by gently inverting.
8. Place tube below sample probe and press blue start paddle.
1. Hold the sample below the sample probe leaving
a gap to the probe tip to allow the sample probe to
aspirate air prior to moving down for sampling.
2. Ensure sample probe touches tube bottom. and
maintains contact until the probe starts to move
back up.
While measuring, the SA screen is locked and navigation to other screens is prohibited. Also, the SA
status indicator light (top right of the SA Results Panel) will display yellow during measurement process.

VETSCAN SA USER’S MANUAL v1.1 SOFTWARE | 20
UA Only Test
1. Enter Patient Information:
Enter Patient ID and select Species (Cat, Dog, Other, Control).
Enter Patient Name and Doctor Name if desired.
2. Enter physical characteristics of the sample (optional):
Use the drop-down option lists to select gross examination fields for the sample (Collection
Method, Color, and Clarity) for the medical record.
3. Enter specific gravity of the sample (optional):
Input specific gravity value of the sample, if applicable, in format 1.0xx. Specific gravity is often
measured using a refractometer.
4. Select Test Type (2 options): UA14, UA10
5. Click Run.
6. Mix urine sample by gently inverting.
7. Confirm Strip Type, Species, and PID (Patient ID) on UA analyzer. Prepare to apply urine with
dropper on UA strip. If incorrect, the UA test can be canceled by pressing the UA Back button.
8. Press Test (Test Tube icon) button to start test. Countdown
timer appears. Perform the next three steps before the timer
reaches 35.
9. Apply urine sample to strip. Use a dropper pipette to
drop urine over entire strip, completely wetting each
pad. Alternatively, strip may be dipped into urine sample,
completely wetting every pad.
10. Blot either long edge of strip on absorbent paper to
remove excess urine. DO NOT blot the top surface of the
pads to avoid contamination. Work quickly to blot excess
urine.
11. Place strip on the UA Strip Tray. The end of the strip
should align with the end of the trough in the Strip Tray,
approximately where the top cover of the UA analyzer
is indented. This step MUST be completed before the
countdown timer reaches 35 as the Strip Tray will begin
to slide into the analyzer. The UA analyzer will time the
reactions and scan when appropriate.