Acea NovoCyte Operating and maintenance instructions

NovoCyte® and NovoCyte® Quanteon Flow Cytometers
QUICK START & SAMPLE ACQUISITION GUIDE
STARTUP
STARTUP
RUN
SAMPLES
QC TEST
1. Check for adequate levels of the NovoFlow®, NovoRinse®, and
NovoClean™ in the fluidic containers. Ensure that each is at
least 1/2 full, and preferably empty waste container.
2. Press the power button on the front panel of the flow
cytometer to turn on the instrument. Launch the
NovoExpress® software on the computer. Startup is
automated and takes approximately 6 minutes. Check the
instrument status as indicated by the LED indicator on the
front panel:
Green = Normal.
The instrument is ready to use.
Orange = Warning.
Click on the status bar in the NovoExpress software to review
the warning message.
Red = Error.
Click on the status bar to review the error message.
Refer to Section 5: Troubleshooting in the Flow Cytometer
Operator’s Guide.
QC TEST
1. Add 1 drop of the NovoCyte QC particles to 0.5 mL of dilution
buffer (0.4 mL PBS and 0.1 mL NovoRinse solution).
2. On the main menu, click on Instrument tab → Under Operation
Section → Click QC Test and follow the software prompts to
complete the test.
3. If the QC Test results display Pass or Acceptable, the instru-
ment may be used to acquire data. If any QC Test results
display “Fail”, refer to Section 5: Troubleshooting in the Flow
Cytometer Operator’s Guide.
SHUTDOWN
1. Press the power button on the front panel of the flow
cytometer. The instrument will automatically perform the
shutdown cleaning process and then power off.
2. When completed the LED indicator will turn off.
FLUIDICS MANAGEMENT
1. The soware will display a warning message instrucng the
user to add more reagents when the volumes of NovoFlow,
NovoClean, and NovoRinse are less than the set thresholds of
0.9 L, 150 mL, and 150 mL, respecvely.
2. To add reagents:
1) Make sure the instrument is Off or in the Ready status.
2) Disconnect the tubing from the container.
3) Bring the container to the sink. Unscrew the cap and add
more reagent to the container.
4) Screw the cap back on, reconnect the quick coupler, and
return the container back to the fluidics staon. Ensure
that the tubing is not twisted or kinked.
5) If the instrument was in the Ready status, click Instrument
tab → Under Fluidics Maintenance → Click Priming in the
soware to prime the system before running samples.
3. Similarly, the soware will warn the user to empty the waste
container when the waste volume exceeds 2.8 L.
1) Follow the steps above to disconnect the waste container
from the instrument. Dispose of the contents according to
local safety guidelines.
2) Add 300 mL of bleach to the waste container and
reconnect the container to the instrument.
SETUP
SAMPLES AND PLOTS
VERIFY
CORRECT PARAMETERS AND
ACQUISITION SETTINGS
Events/Volume/Flow Rate/Threshold
RUN
COMPENSATION
CONTROLS
(if applicable)
© 2018 ACEA Biosciences, Inc. All rights reserved.
6779 Mesa Ridge Rd. Ste 100 San Diego, CA 92121 | 1.866.308.2232 | www.aceabio.com

NovoCyte® and NovoCyte® Quanteon Flow Cytometers
QUICK MAINTENANCE GUIDE
REPLACE FLUIDICS SYSTEM CONSUMABLES
© 2018 ACEA Biosciences, Inc. All rights reserved.
6779 Mesa Ridge Rd. Ste 100 San Diego, CA 92121 | 1.866.308.2232 | www.aceabio.com
1. The sheath fluid in-line filter is to be replaced every 2 months or when prompted by the NovoExpress® software, whichever comes
first. For all other filters (waste and inlet filters, see below), they should be replaced by following the prompts from the NovoExpress
software when the maximum accumulated run time has been reached or click Instrument tab → Under Operation Section → Click
Replace Fluidic System Consumables. You can see how much time has been accumulated since last filter change by clicking Instrument tab
→Under Instrument Section → Click Information. The notification will appear when 176 hours of instrument use has reached.
2. Replace the NovoFlow In-Line Filter as described in the Flow Cytometer Maintenance Guide or follow the notification prompt to change
all of the filters in the fluidic system listed in the table below.
Clean the SIP (Sample Injecon Probe) and the SIP Wash Apparatus
1. Click Instrument → Shut Down on the NovoExpress software.
2. Select Clean sample injection probe option and then click Yes button in the prompted window. The NovoExpress software will
guide you to conduct the SIP cleaning procedure.
3. Follow the instructions in the software, remove the sample tube from the tube holder and click OK
4. After clicking the OK button, the sample injection probe will move downward. Prepare a cotton swab or a damp soft cloth with
ACEA NovoClean solution (1X) and gently wipe the outer surface of the sample injection probe.
5. Click OK button after cleaning.
6. Please put a tube of filtered DI water or ACEA NovoRinse solution (1X) in the sample holder and click the OK button again. The
instrument will shut down automatically.
7. To clean the SIP wash apparatus while the instrument is OFF, prepare a cotton swab soaked with ACEA NovoClean solution (1X).
Wipe the SIP wash apparatus’ lower surface back and forth until it’s clean
8. Prepare a damp cloth soaked with DI water. Wipe the SIP cleaning apparatus back and forth at least five times.
9. Power on the NovoCyte Quanteon instrument and start the NovoExpress software. After the instrument is Ready:
-Click Instrument tab → Under Fluidics Maintenance Section → Click Unclog to flush the SIP.
-Click Instrument tab → Under Fluidics Maintenance Section → Click Priming to prime the fluidic system.
Note: Handle the SIP and the SIP wash apparatus carefully when cleaning to avoid damage. Refer to Section 1.1 of Flow Cytometer
Maintenance Guide for additional information.
PREVENTIVE MONTHLY MAINTENANCE
Fluidic System Consumable Items Quanty Catalog Number
NovoFlow®Sheath Fluid In-line Filter 1 EA 2030002
Waste Filter 1 EA 2030003
NovoFlow®Sheath Fluid Inlet Filter 1 EA 2030001
NovoRinse®Soluon Inlet Filter 1 EA 2030005
NovoCleanTMSoluon Inlet Filter 1 EA 2030007