Acea NovoCyte User manual

NovoCyte®Flow Cytometer
Mul-opon Conguraon Guide

2
NovoCyte Mul-opon Conguraon Guide
ACEA NovoCyte® system provides versality and exibility with its mul-opon opcal conguraon capabilies. Researchers
can expand their detecon capabilies to meet their experiment or panel design using the NovoCyte opcal conguraon tools.
These tools are accessible through the NovoExpress® soware user interface. Standard or default instrument opcal conguraon
can be changed and modied on the y with spare opcal lters and dichroic mirrors.
This guide provides an outline for the available conguraon opons for dierent models of NovoCyte ow cytometer.
3005 Model
NovoCyte Violet/Blue/Red System with baseline conguraon of 3 lasers (405 nm, 488 nm, 640 nm) and 6 PMTs with ≥3 red
channels.
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm 640 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 660/20 650LP Qdot 655 PE-Cy5 APC
5 725/40 685LP Qdot 705 PerCP-eFluor 710 Alexa Fluor 700
6 780/60 757LP Qdot 800 PE-Cy7 APC-Cy7
14 FL Channels 6 5 3

3
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm 640 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 660/20 650LP Qdot 655 PE-Cy5 APC
5 695/40 685LP Qdot 705 PE-Cy5.5 Alexa Fluor 700
6 780/60 735LP Qdot 800 PE-Cy7 APC-Cy7
14 FL Channels 6 5 3
Opcal Conguraon Opon 2
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm 640 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 660/20 650LP Qdot 655 PE-Cy5 APC
4 695/40 685LP Qdot 705 PE-Cy5.5 Alexa Fluor 680
5 725/40 705LP BV711 PerCP-eFluor 710 Alexa Fluor 700
6 780/60 757LP Qdot 800 PE-Cy7 APC-Cy7
15 FL Channels 6 5 4

4
3000 Model
NovoCyte Violet/Blue/Red System with baseline conguraon of 3 lasers (405 nm, 488 nm, 640 nm) and 6 PMTs with 2 red
channels.
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm 640 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 615/20 600LP Qdot 605 PE-Texas Red
5 675/30 650LP Qdot 655 PerCP APC
6 780/60 735LP Qdot 800 PE-Cy7 APC-Cy7
13 FL Channels 6 5 2
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm 640 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 615/20 600LP Qdot 605 PE-Texas Red
5 660/20 650LP Qdot 655 PE-Cy5 APC
6 780/60 735LP Qdot 800 PE-Cy7 APC-Cy7
13 FL Channels 6 5 2

5
2070R Model
NovoCyte Blue/Red System with 2 lasers (488 nm, 640 nm) and 5 PMTs.
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 615/20 600LP PE-Texas Red
4 660/20 650LP PE-Cy5 APC
5 780/60 735LP PE-Cy7 APC-Cy7
7 FL Channels 5 2
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 660/20 650LP PE-Cy5 APC
4 725/40 685LP PerCP-eFluor 710 Alexa Fluor 700
5 780/60 757LP PE-Cy7 APC-Cy7
8 FL Channels 5 3

6
NovoCyte Blue/Red System with 2 lasers (488 nm, 640 nm) and 4 PMTs.
2060R Model
Opcal Conguraon Opon 2
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 660/20 650LP PE-Cy5 APC
4 695/40 685LP PE-Cy5.5 Alexa Fluor 700
5 780/60 735LP PE-Cy7 APC-Cy7
8 FL Channels 5 3
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 675/30 650LP PerCP APC
4 780/60 735LP PE-Cy7 APC-Cy7
6 FL Channels 4 2

7
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 660/20 650LP PE-Cy5 APC
4 780/60 735LP PE-Cy7 APC-Cy7
6 FL Channels 4 2
Opcal Conguraon Opon 2
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 660/20 650LP PE-Cy5 APC
3 695/40 685LP PE-Cy5.5 Alexa Fluor 700
4 780/60 735LP PE-Cy7 APC-Cy7
7 FL Channels 4 3

8
NovoCyte Blue/Red System with 2 lasers (488 nm, 640 nm) and 3 PMTs.
2000R Model
Opcal Conguraon Opon 3
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 660/20 650LP PE-Cy5 APC
3 725/40 685LP PerCP-eFluor 710 Alexa Fluor 700
4 780/60 757LP PE-Cy7 APC-Cy7
7 FL Channels 4 3
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 675/30 650LP PerCP APC
4 FL Channels 3 1

9
NovoCyte Violet/Blue System with 2 lasers (405 nm, 488 nm) and 6PMTs.
2110V Model
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
488 nm 640 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 660/20 650LP PE-Cy5 APC
4 FL Channels 3 1
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 660/20 650LP Qdot 655 PE-Cy5
5 695/40 685LP Qdot 705 PE-Cy5.5
6 780/60 735LP Qdot 800 PE-Cy7
11 FL channels 6 5

10
Opcal Conguraon Opon 2
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 615/20 600LP Qdot 605 PE-Texas Red
5 660/20 650LP Qdot 655 PE-Cy5
6 780/60 735LP Qdot 800 PE-Cy7
11 FL channels 6 5
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 615/20 600LP Qdot 605 PE-Texas Red
5 695/40 650LP Qdot 705 PE-Cy5.5
6 780/60 735LP Qdot 800 PE-Cy7
11 FL channels 6 5

11
NovoCyte Violet/Blue System with 2 lasers (405 nm, 488 nm) and 5 PMTs.
2090V Model
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 695/40 650LP Qdot 705 PE-Cy5.5
5 780/60 735LP Qdot 800 PE-Cy7
9 FL Channels 5 4
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 615/20 600LP Qdot 605 PE-Texas Red
5 780/60 735LP Qdot 800 PE-Cy7
9 FL Channels 5 4

12
Opcal Conguraon Opon 2
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 660/20 650LP Qdot 655 PE-Cy5
5 780/60 735LP Qdot 800 PE-Cy7
9 FL Channels 5 4
Opcal Conguraon Opon 3
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 660/20 650LP Qdot 655 PE-Cy5
4 695/40 685LP Qdot 705 PE-Cy5.5
5 780/60 735LP Qdot 800 PE-Cy7
9 FL Channels 5 4

13
NovoCyte Violet/Blue System with 2 lasers (405 nm, 488 nm) and 4 PMTs.
2070V Model
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 695/40 650LP Qdot 705 PE-Cy5.5
7 FL Channels 4 3
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
405 nm 488 nm
1 445/45 472SP Pacic Blue
2 530/30 505LP AmCyan FITC
3 572/28 555LP Pacic Orange PE
4 660/20 650LP Qdot 655 PE-Cy5
7 FL Channels 4 3

14
NovoCyte Blue System with 1 laser (488 nm) and 3 PMTs.
1000 Model
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 675/30 650LP PerCP
3 FL Channels 3
Opcal Conguraon Opon 1
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 660/20 650LP PE-Cy5
3 FL Channels 3

15
NovoCyte Blue System with 1 laser (488 nm) and 4 PMTs.
1040 Model
Opcal Conguraon Opon 2
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 695/40 650LP PE-Cy5.5
3 FL Channels 3
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 675/30 650LP PerCP
4 780/60 735LP PE-Cy7
4 FL Channels 4

16
Opcal Conguraon 1
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 615/20 600LP PE-Texas Red
4 780/60 735LP PE-Cy7
4 FL Channels 4
Opcal Conguraon 2
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 660/20 650LP PE-Cy5
4 780/60 735LP PE-Cy7
4 FL Channels 4

17
NovoCyte Blue System with 1 laser (488 nm) and 5 PMTs.
1050 Model
Opcal Conguraon 3
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 695/40 650LP PE-Cy5.5
4 780/60 735LP PE-Cy7
4 FL Channels 4
Default Opcal Conguraon
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 615/20 600LP PE-Texas Red
4 675/30 650LP PerCP
5 780/60 735LP PE-Cy7
5 FL Channels 5

18
Opcal Conguraon 1
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 660/20 650LP PE-Cy5
4 695/40 680LP PE-Cy5.5
5 780/60 735LP PE-Cy7
5 FL Channels 5
Opcal Conguraon 2
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 615/20 600LP PE-Texas Red
4 660/20 650LP PE-Cy5
5 780/60 735LP PE-Cy7
5 FL Channels 5

19
NovoCyte Red/Yellow/Blue System with 3 lasers (640 nm, 561 nm, 488 nm) and 6 PMTs.
3000RYB Model
Opcal Conguraon 3
Detector BP Filter Dichroic Mirror Laser
488 nm
1 530/30 505LP FITC
2 572/28 555LP PE
3 615/20 600LP PE-Texas Red
4 695/40 650LP PE-Cy5.5
5 780/60 735LP PE-Cy7
5 FL Channels 5
Default Opcal Conguraton
Detector BP Filter Dichroic Mirror Laser
488 nm 561 nm 640 nm
1 530/30 - FITC
2 586/20 572LP EYFP PE
3 615/20 600LP PI PE-Texas Red
4 660/20 650LP PerCP PE-Cy5 APC
5 695/40 685LP PerCP-Cy5.5 PE-Cy5.5 Alexa Fluor 700
6 780/60 735LP PE-Cy7 APC-Cy7
13 FL Channels 5 5 3

20
Opcal Conguraton Opon 1
Detector BP Filter Dichroic Mirror Laser
488 nm 561 nm 640 nm
1 530/30 - FITC
2 586/20 572LP EYFP PE
3 615/20 600LP PI PE-Texas Red
4 660/20 650LP PerCP PE-Cy5 APC
5 725/40 685LP PerCP-eFluor 710 PE-Alexa Fluor 700 Alexa Fluor 700
6 780/60 757LP PE-Cy7 APC-Cy7
13 FL Channels 5 5 3
Opcal Conguraton Opon 2
Detector BP Filter Dichroic Mirror Laser
488 nm 561 nm 640 nm
1 530/30 - FITC
2 586/20 572LP EYFP PE
3 660/20 650LP PerCP PE-Cy5 APC
4 695/40 685LP PerCP-Cy5.5 PE-Cy5.5 Alexa Fluor 680
5 725/40 705LP PerCP-eFluor 710 PE-Alexa Fluor 700 Alexa Fluor 700
6 780/60 757LP PE-Cy7 APC-Cy7
14 FL Channels 5 5 4