Acea RTCA SP Instrument User manual

RTCA SP Instrument
Operator’s Manual Version January 2013

3
Table of Content
Prologue 5
I. Revision History .................................................................................................................................................5
II. Contact Addresses ...........................................................................................................................................5
III. Declaration of Conformity............................................................................................................................6
IV. Warranty .................................................................................................................................................................6
V. Trademarks ...........................................................................................................................................................6
VI. Intended Use........................................................................................................................................................6
VII. License Statements for the Instrument................................................................................................7
VIII. Software License Agreement.....................................................................................................................7
IX. Preamble................................................................................................................................................................7
X. Use of the Instrument Operator’s Manual ..........................................................................................8
XI. Conventions Used in this Manual............................................................................................................8
XII. Warnings and Precautions........................................................................................................................ 10
XIII. Disposal of the Instrument ....................................................................................................................... 12
A Overview 13
1. Introduction....................................................................................................................................................... 13
1.1 RTCA SP Instrument ......................................................................................................................................... 14
2. Specifi cations of the Instrument ........................................................................................................... 17
2.1 Specifi cations of the RTCA Analyzer.......................................................................................................... 17
2.2 Specifi cations of the RTCA SP Station ...................................................................................................... 17
2.3 Specifi cations of the RTCA Control Unit................................................................................................... 17
2.4 Specifi cations of the E-Plate 96 ................................................................................................................... 18
2.5 Specifi cations of the RTCA Resistor Plate 96.......................................................................................... 18
B System Description 19
1. System Package ............................................................................................................................................. 19
2. System Description....................................................................................................................................... 20
2.1 Description of the RTCA Analyzer............................................................................................................... 21
2.2 Description of the RTCA SP Station ........................................................................................................... 22
2.2.1 Cradle Pocket and Clamp Plate.................................................................................................................... 23
2.2.2 RTCA Protector Shield 96 and RTCA Contact Pins 96......................................................................... 23
2.3 RTCA Control Unit ............................................................................................................................................. 24
2.4 E-Plate 96 and RTCA Resistor Plate 96 ..................................................................................................... 25
2.5 RTCA Frame 96................................................................................................................................................... 26
2.6 RTCA Software.................................................................................................................................................... 27
2.7 RTCA Cleaning and Replacement Equipment ........................................................................................ 27
3. Installation ......................................................................................................................................................... 28
3.1 Installation Warnings........................................................................................................................................ 28
3.2 Unpacking ............................................................................................................................................................ 28
3.3 Space and Power Requirements.................................................................................................................. 29
3.4 Environmental Requirements........................................................................................................................ 29
3.5 Installation of the RTCA SP Instrument .................................................................................................... 29
3.6 Resistor Plate Verifi cation of the RTCA SP Instrument........................................................................ 31

4RTCA SP Instrument Operator’s Manual
Table of Content
C Operation 35
1. Introduction....................................................................................................................................................... 35
2. Instrument Start-Up and Warm-Up ..................................................................................................... 35
3. Preparing and Starting a Run on the RTCA SP Instrument .................................................... 36
3.1 Running a Quick Experiment ........................................................................................................................ 36
3.2 Important Tips for Using the RTCA SP Instrument .............................................................................. 42
D Maintenance and Care 44
1. General Maintenance .................................................................................................................................. 44
2. Cleaning Instructions................................................................................................................................... 45
2.1 General Cleaning ............................................................................................................................................... 45
2.2 Further Recommendations............................................................................................................................. 45
3. Cleaning and Exchanging the RTCA Contact Pins 96 ............................................................... 46
4. Exchanging Fuses .......................................................................................................................................... 50
E Appendix 52
1. Troubleshooting............................................................................................................................................... 52
1.1 I started an experiment and there was an error message,
“Cannot fi nd instrument”, what happened? ........................................................................................... 52
1.2 I started an experiment and there was a sound and error message,
“No Station”, what happened?...................................................................................................................... 53
1.3 I started an experiment. There was an error message,
“ACK communication lost.” What happened?......................................................................................... 54
1.4 How do I reinitialize the RTCA SP Instrument after a power failure? ............................................ 55
1.5 The Cell Index signal is signifi cantly lower than usual. Why? .......................................................... 55
1.6 The Cell Index signal decreases with time, but there was no compound added. Why? ........ 56
1.7 The Cell Index signal is signifi cantly higher than usual. Why?......................................................... 56
1.8 I did a background measurement. The RTCA Software indicated that
many wells may have background problems. What should I do?................................................... 57
1.9 I placed an E-Plate 96 into the cradle of the RTCA SP Station.
The Device Indicating LED did not turn green. What should I do?................................................ 58
1.10 I am contacting the local ACEA representative or ACEA Technical Support.
What data fi les should be sent? ................................................................................................................. 58
2. Ordering Information.................................................................................................................................... 59

5
Prologue
Prologue
I. Revision History
Version Revision Date
1.0 June 2008
2.0 September 2008
3.0 March 2009
3.1 (editorial change) November 2009
4.0 January 2013
© Copyright 2013, ACEA Biosciences, Inc. All rights reserved. Information in this docu-
ment is subject to change without notice. No part of this document may be reproduced
or transmitted in any form or by any means, electronic or mechanical, for any purpose,
without the express written permission of ACEA Biosciences, Inc..
Questions or comments regarding the contents of this manual can be directed to the
address below or to your local ACEA representative.
ACEA Biosciences, Inc.
Customer Support
6779 Mesa Ridge Rd., Suite 100
San Diego, CA 92121
USA
Every eort has been made to ensure that all the information contained in the RTCA SP
Instrument Operator’s Manual is correct at the time of printing.
However, ACEA Biosciences, Inc. reserves the right to make any changes necessary with-
out notice as part of ongoing product development.
II. Contact Addresses
Manufacturer ACEA Bio (Hangzhou) Co, LTD.
Building 5, West Lake Technology & Economy Park.
2# Xiyuan 5 Road Sandun, Hangzhou
Zhejiang, China, 310030
Distribution in China ACEA Bio (Hangzhou) Co, LTD.
Building 5, West Lake Technology & Economy Park.
2# Xiyuan 5 Road Sandun, Hangzhou
Zhejiang, China, 310030
Distribution in USA ACEA Biosciences, Inc.
6779 Mesa Ridge Rd., Suite 100
San Diego, CA 92121
USA
International Distribution For a complete listing of international distributors,
contact ACEA Biosciences, Inc or visit
www.aceabio.com
Revision History

6RTCA SP Instrument Operator’s Manual
III. Declaration of Conformity
The instrument meets the requirements laid down in Council Directive
2004/108/EC relating to “Electromagnetic Compatibility” and Council
Directive
2006/95/EC relating to “Low Voltage Equipment”. The following
standards were applied: IEC/EN 61326 (EMC) and IEC/EN 61010-1 (Safety).
UL 61010-1 Electrical Equipment for Measurement, Control and Laboratory
Use; Part 1: General Requirements CAN/CSA-C22.2 No. 61010-1 (Second
Edition) — Safety Requirements for Electrical Equipment for Measurement,
Control and Laboratory Use; Part 1: General Requirements
IV. Warranty
Information on warranty conditions are specied in the sales contract. Contact your
ACEA representative for further information.
Any unauthorized modication of the instrument invalidates the guarantee and service
contract.
V. Trademarks
XCELLIGENCE, E-PLATE and ACEA BIOSCIENCES are registered trademarks of ACEA
Biosciences, Inc.in the US.
Other brands or product names are trademarks of their respective holders.
VI. Intended Use
e RTCA (Real-Time Cell Analyzer) SP (Single Plate) Instrument is intended for label-
free, real-time, automated monitoring of cell status in a variety of cell-based assays, using
proprietary micro-electronic sensor technology developed by ACEA Biosciences. It can
be used for both high throughput screening and research laboratory environments.
e RTCA SP Instrument is intended for life science research and must be used exclusively
by laboratory professionals who are trained in laboratory techniques and have studied the
instructions for use of this instrument. e RTCA SP Instrument is not intended for use
in diagnostic procedures.
Declaration of Conformity

7
Prologue
VII. License Statements for the Instrument
is RTCA SP System is a real-time cell based assay system covered by US pat-
ent No.7,192,752, 7,459,303, 7,468,255, 7,470,533, 7,560,269, 7,732,127, 7,867,108,
8,026,080, 8,041,505; European patent applications 03748948.1, 03751801.6 and
04801001.1, 05722991.6, 05786773.1, and other patents and patent applications owned
by ACEA Biosciences, Inc..
VIII. Software License Agreement
An envelope containing all license and end user agreements is packaged with the RTCA
Control Unit. It contains:
- RTCA Soware End User License Agreement
- End User License Agreement for Microso Windows 7 Professional
- End User License Agreement for Microso Oce Home & Business 2010
- Microso Oce Home & Business 2010 Product Key
- End User License Agreement for AVG AntiVirus FREE Soware
Please comply with all statements made in these documents.
IX. Preamble
e RTCA SP Instrument Operator’s Manual has to be used in conjunction with the
corresponding RTCA Soware Manual. Before setting-up operation of the RTCA SP
Instrument, it is important to read this Operator’s Manual and the corresponding RTCA
Soware Manual thoroughly and completely. Failure to observe the instructions contained
in this manual could be hazardous.
License Statements for the Instrument

8RTCA SP Instrument Operator’s Manual
X. Use of the Instrument Operator’s Manual
is Operator’s Manual describes the operation of the RTCA SP Instrument and contains
the following chapters:
Chapter A Overview contains a short introduction to the operation of the RTCA SP In-
strument and describes the instrument’s specications.
Chapter B System Description contains the description of the RTCA SP instrument, and
the instructions on the installation of the instrument.
Chapter C Operation describes the operating procedures for the RTCA SP Instrument.
Chapter D Maintenance and Care describes the maintenance procedures that are requi-
red for the RTCA SP Instrument.
Chapter E Appendix contains trouble shooting and ordering information of the RTCA
SP Instrument, accessories and consumables.
XI. Conventions Used in this Manual
Text Conventions
To impart information that is consistent and memorable, the following text conventions
are used in this Operator’s Manual:
Numbered Listing Describes the steps in a procedure that must be performed
in the order listed.
Italic type, gold Points to a different chapter in this Operator’s Manual
which should be referred to for better understanding.
Italic type Describes buttons, icons or functions when operating
the RTCA Software. In addition, important notes and
information notes are shown in italic type.
Use of the Instrument Operator’s Manual

9
Prologue
Symbols
In this Operator’s Manual symbols are used as a visual signal to highlight important points.
Symbol Heading Description
WARNING This symbol is used to indicate that noncompliance
with instructions or procedures could lead to
physical injury or even death or could cause
damage to the instrument. Consult this Operator’s
Manual.
BIOHAZARD This symbol indicates that certain precautions must
be taken when working with potentially infectious
samples material.
IMPORTANT NOTE Information critical to the success of the procedure
or use of the product.
INFORMATION NOTE Additional information about the current topic or
procedure.
 Table continued on next page.
End of table.
e following symbols appear on the instrument:
Symbol Heading Description
AC Alternating Current
ON/OFF Power ON
Power OFF
GND Protective Earth Ground
FUSE Fuse: Refer to included safety statements
WEEE Electrical and electronic equipment marked with
this symbol are covered by the European Directive
WEEE.
The symbol denotes that the equipment must not
be disposed of in the municipal waste system.
BIOHAZARD This symbol indicates that certain precautions must
be taken when working with potentially infectious
material.
COM COM Communications Port
EPS EPS E-Plate Station Port
Conventions Used in this Manual

10 RTCA SP Instrument Operator’s Manual
XII. Warnings and Precautions
Handling Requirements
e RTCA SP Instrument must only be used by trained and skilled personnel. It is essential
that the following safety information required for installation and operation of the RTCA
SP Instrument is carefully read and observed. Please ensure that this safety information is
accessible to every employee working with the RTCA SP Instrument.
 Follow all safety instructions printed on or attached to the instrument.
 Observe all general safety precautions applicable to electrical instruments.
 Do not access any electrical parts while the RTCA SP Instrument is connected to the
mains.
 Never touch switches or power cords with wet hands.
 Do not open the housing of the RTCA SP Instrument.
 Never clean the instrument without turning the instrument power switch o and
disconnecting the power cord.
 Only authorized service personnel are allowed to perform service operations or repairs
required for this instrument.
 Do not open the clamp plate of the RTCA SP Station during measurement as this will
cause an open circuit, leading to an error message in the RTCA Soware.
For your own safety, please consider all biological material as potentially infectious.
Handling and disposal of such material should be performed according to local
safety guidelines. Spills should be immediately disinfected with an appropriate
disinfectant solution to avoid spreading infection to, and contamination of,
laboratory personnel or equipment. When working with potentially infectious
material, please do always use protective gloves (powder-free).

Please refer to the section on Maintenance and Care for instructions on cleaning the
RTCA SP Instrument.
Warnings and Precautions