Agilent Technologies 1290 Infinity II 2D-LC Solution User manual

Agilent 1290 Infinity II 2D-LC Solution
MassHunter Acquisition for TOF and Q-TOF
User Guide
2D-LC User Guide

2D-LC User Guide
Notices
Document Information
Document No: D0004828 Rev. A
Edition: 05/2021
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2D-LC User Guide 3
In This Book
In This Book
This manual covers the Agilent 1290 Infinity II 2D-LC Solution for MassHunter
TOF and Q-TOF.
1Introduction
This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.
2 Concepts of 2D-LC
This chapter describes the concepts of Agilent 1290 Infinity II 2D-LC Solution.
3 Compatibility Matrix
This chapter provides information about installation and execution prerequisites
regarding hardware, firmware, and the operating system. Agilent 1290 Infinity II
2D-LC Solution.
4 Installation
This chapter describes the hardware and software installation of the Agilent
1290 Infinity II 2D-LC Solution. The 2D-LC instrument can be used with the
software described in this document. The installation instructions are valid for
the modes standard heart-cutting, multiple heart-cutting, high resolution
sampling and comprehensive 2D-LC.
5 2D-LC Data Acquisition in MassHunter Workstation 11
This chapter provides information about 2D-LC data acquisition in MassHunter
Workstation 11.
6MethodParameters
This chapter provides background information on method parameters. It helps to
optimize methods in Agilent 1290 Infinity II 2D-LC Solution in the modes standard
heart-cutting, multiple heart-cutting, high resolution sampling and
comprehensive 2D-LC.

In This Book
2D-LC User Guide 4
7 Method Development of Active Solvent Modulation (ASM)
This chapter provides information on how to develop methods when using Active
Solvent Modulation (ASM).
8 Run the System
This chapter describes how to run the Agilent 1290 Infinity II 2D-LC Solution in
the modes standard heart-cutting, multiple heart-cutting, high resolution
sampling and comprehensive 2D-LC with the driver-based 2D-LC Solution.
9 Data Analysis
This chapter provides information on how to analyze 2D-LC data with software.
10 Troubleshooting and Diagnostics
This chapter gives an overview about the troubleshooting and diagnostic
features and the different user interfaces.
11 Error Information
This chapter describes the meaning of error messages, and provides information
on probable causes and suggested actions how to recover from error conditions.
12 Maintenance
This chapter describes the maintenance of the 2D-LC Solution.
13 Parts for Maintenance
This chapter provides information on parts material required for the solution.
14 Theoretical Background
This chapter gives the theoratical background of 2D-LC and describes the
system components (soft- and hardware) of the Agilent 1290 Infinity II 2D-LC
Solution.
15 Appendix
This chapter provides addition information on safety, legal and web.

2D-LC User Guide 5
Contents
1Introduction10
Product Description 11
Features 12
Terms related to 2D-LC 14
2 Concepts of 2D-LC 15
Concepts of 2D-LC 16
Heart-Cutting 2D-LC (LC-LC) 18
Multiple Heart-Cutting and High Resolution Sampling 2D-LC 19
Comprehensive 2D-LC (LCxLC) 30
Triggering of 2D-LC 32
Active Solvent Modulation (ASM) 35
3 Compatibility Matrix 45
Supported Chromatographic Data Systems 46
Supported Drivers 47
Supported Operating Systems 48
Supported Firmware 49
Available Languages 50
PC Requirements 51
Licensing 52
4 Installation 53
Hardware Installation 54
Software Installation 89

2D-LC User Guide 6
5 2D-LC Data Acquisition in MassHunter Workstation 11 103
Start the Data Acquisition Software 104
Overview 2D-LC in MassHunter Acquisition 11 106
Instrument Status 107
2D-LC User Interface 108
2D-LC Valves Online Monitor in the 2D-LC User Interface 114
Method Editor Window 116
Sample Run Window 117
Worklist Window 118
Tune Window 119
Instrument Details 120
Log book in MassHunter Acquisition 11 123
6 Method Parameters 125
Method Editor 2D-LC 126
Set the 2D-LC Method parameters 127
Preview (2D-LC) 154
Set up a Peak-Based Experiment Graphically 168
Setup 2D Gradient Graphically 173
Setup Second Dimension Gradient with the Graphical User Interface 175
Additional Information 177
7 Method Development of Active Solvent Modulation (ASM) 185
Method Development of Active Solvent Modulation (ASM) 186
Method Parameters 187
Optimize the Dilution by Using ASM Capillaries 188
Optimize the Sample Loop Flush 189
Include the ASM Phase to the 2D Gradient 190
Optimize Dilution Through Method Settings 191

2D-LC User Guide 7
8 Run the System 192
Familiarization to Start a System Run 193
Prepare the 2D-LC System 194
Configure the 2D-LC System 195
Checkout Familiarization Procedure 198
Prepare the Experiment 199
Run the Experiment 201
9 Data Analysis 229
2D-LC Data Analysis/Data Evaluation for MassHunter 230
GC Image Basic Information 256
10 Troubleshooting and Diagnostics 275
Overview of the Module’s Indicators and Test Functions 276
User Interfaces 277
Agilent Lab Advisor Software 278
Lab Advisor Instrument Control 280
Lab Advisor Service &Diagnostic 286
The Basic Principle of Troubleshooting 294
Recommended Tests to Conclude Troubleshooting 304
11 Error Information 305
What Are Error Messages 306
General Error Messages 307
Module-Specific Error Messages 313

2D-LC User Guide 8
12 Maintenance 317
Introduction to Maintenance 318
Warnings and Cautions 319
Overview of Maintenance 321
Cleaning the Module 322
Correcting Leaks 323
Replace Valve Heads 324
Replacing Parts of the Valve Head 330
Replacing the Fuses of the Infinity Valve Drive 332
13 Parts for Maintenance 334
2D-LC Loops 335
2D-LC Capillaries 336
ASM Capillaries 337
Pressure Release Kit 338
2D-LC Easy Starter Kit 339
Valve Drive Parts 340
Valve Driver Parts Infinity II 341
Valve Head Parts 342
Vale Options Overview (for 2D-LC) 344
MS Diverter Valve 348
Valve Options Overview (for G7116B) 350
Additional Heater Devices 352
Accessories and Consumables (for G7116B) 354
InfinityLab Quick Connect and Quick Turn Fittings 356
Capillary Kits 360
Important Customer Web Links 361

2D-LC User Guide 9
14 Theoretical Background 362
Theoretical basis of 2D-LC 363
2D as detector 371
Successful Mode Combinations 373
Solvent Elution Modes 374
Practical Issues 380
15 Appendix 382
General Safety Information 383
Waste Electrical and Electronic Equipment (WEEE) Directive 389
Radio Interference 390
Sound Emission 391
Solvent Information 392
Further Information 393
Agilent Technologies on Internet 394

2D-LC User Guide 10
1Introduction
Product Description 11
Features 12
Terms related to 2D-LC 14
This chapter describes the product of Agilent 1290 Infinity II 2D-LC Solution.