Agilent Technologies 2100 Bioanalyzer System Installation and operating manual

Agilent 2100 Bioanalyzer
Installation and Safety Guide

Notices
© Agilent Technologies, Inc.
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Manual Part Number
G2938-90007 Rev B
Edition
Printed in Germany
Agilent Technologies
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This product may be used as a com-
ponent of an in vitro diagnostic sys-
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the appropriate authorities and com-
plies with the relevant regulations.
Otherwise, it is intended only for gen-
eral laboratory use.
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Safety Notices
CAUTION
A CAUTION notice denotes a
hazard. It calls attention to an
operating procedure, practice, or
the like that, if not correctly per-
formed or adhered to, could
result in damage to the product
or loss of important data. Do not
proceed beyond a CAUTION
notice until the indicated condi-
tions are fully understood and
met.
WARNING
A WARNING notice denotes a
hazard. It calls attention to an
operating procedure, practice,
or the like that, if not correctly
performed or adhered to, could
result in personal injury or
death. Do not proceed beyond a
WARNING notice until the indi-
cated conditions are fully under-
stood and met.

3
Contents
Contents
1 Safety Information 5
Power Cords 7
Operation 8
Safety Symbols 9
Laser Safety 10
Chemical and Biological Safety 11
Legal Notice 13
2 Site Requirements for the Agilent 2100 Bioanalyzer 15
Power Considerations 16
Bench space 17
Environment 18
Sound Emission 19
Physical Specifications of the Agilent 2100 Bioanalyzer 20
3 Unpacking the Agilent 2100 Bioanalyzer System 21
Damaged Packaging 22
4 Installing the Agilent 2100 Bioanalyzer System 25
Setting up PC and Printer 26
Setting up the Agilent 2100 Bioanalyzer 27
5 Starting the Agilent 2100 Bioanalyzer 31
Turning on the Agilent 2100 Bioanalyzer 32
Connecting the Bioanalyzer via the USB/Serial Cable 33
Starting the Agilent 2100 Bioanalyzer Software 34
Preparing the Assay 35
6 Maintenance of the Agilent 2100 Bioanalyzer 37
7 Spare Parts and Accessories 39

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Contents

5
Agilent Technologies
1
Safety Information
Power Cords 7
Operation 8
Safety Symbols 9
Laser Safety 10
Chemical and Biological Safety 11
Legal Notice 13
The following general safety precautions must be observed during all phases of
operation, service, and repair of the Agilent 2100 Bioanalyzer.
All safety instructions should be read and understood before installation,
operation and maintenance of the instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture and intended use of the instrument.
Agilent Technologies assumes no liability for the customer’s failure to comply
with these requirements.
WARNING
A WARNING notice denotes a hazard.
It calls attention to an operating procedure, practice, or the like that, if not correctly
performed or adhered to, could result in personal injury or death.
➔Do not proceed beyond a WARNING notice until the indicated conditions are fully
understood and met.

6
1Safety Information
CAUTION
A CAUTION notice denotes a hazard.
It calls attention to an operating procedure, practice, or the like that, if not correctly
performed or adhered to, could result in damage to the product or loss of important
data.
➔Do not proceed beyond a CAUTION notice until the indicated conditions are fully
understood and met.
NOTE
Instrument Safety: This is a Safety Class I instrument (provided with a terminal for
protective grounding) and has been manufactured and tested according to international
safety standards.

7
Safety Information 1
Power Cords
Power Cords
Different power cords are offered as options with the Agilent 2100
Bioanalyzer. The female end of all power cords is identical. It plugs into the
power-input socket at the rear of the instrument. The male end of each power
cord is different and designed to match the wall socket of a particular country
or region.
WARNING
Electric Shock
The absence of ground connection and the use of an unspecified power cord can
lead to electric shock or short circuit.
➔Never operate your instrument from a power outlet that has no ground connection.
➔Never use a power cord other than the Agilent Technologies power cord designed
for your region.
➔Never use cables other than those supplied by Agilent Technologies to ensure
proper functionality and compliance with safety or EMC regulations.

8
1Safety Information
Operation
Operation
Before the instrument is switched on, all protective ground terminals,
extension cords, auto-transformers, and devices connected to it must be
connected to a protective ground socket. Any interruption of the protective
grounding will cause a potential shock hazard that could result in serious
personal injury. Whenever it is likely that the protection has been impaired,
the instrument must be made inoperative and be secured against any intended
operation.
To operate the instrument safely:
•Do not remove any cover of the Agilent 2100 Bioanalyzer
•Avoid any maintenance of the instrument under voltage
•Do not let liquid drip into the Agilent 2100 Bioanalyzer. It could cause a
shock or it could damage the Agilent 2100 Bioanalyzer.
•Do not replace components of the instruments (e.g. electrode cartridge or
pressure cartridge) with power turned on.
•Do not operate the instrument in the presence of flammable gases or fumes.
Operation of any electrical instrument in such an environment constitutes a
definite safety hazard.
•Do not install substitute parts or make any unauthorized modification to
the instrument.
WARNING
Ensure the proper usage of the equipment
The protection provided by the equipment may be impaired.
➔The operator of this instrument is advised to use the equipment in a manner as
specified in this manual.

9
Safety Information 1
Safety Symbols
Safety Symbols
Symbol Description
Electrosensitive device.
The apparatus is marked with this symbol when the user should refer to the Installation and Safety
Manual in order to prevent risk of harm to the operator and to protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
Laser Radiation. When open avoid exposure to beam.

10
1Safety Information
Laser Safety
Laser Safety
The Agilent 2100 Bioanalyzer is classified as a "Laser Class 1" product
(IEC825-1, CFR1040.10). During normal operation of the Agilent 2100
Bioanalyzer no laser light is accessible to the user.
When the laser is in use, the laser light source emits light at a power that may
be harmful to the eyes. To prevent operator exposure to potentially harmful
laser light, an interlock mechanism turns off the laser whenever the lid of the
Agilent 2100 Bioanalyzer is not closed. Furthermore, the lid can't be
completely opened and has a black light absorbing surface in order to prevent
any potential laser light reflection.
WARNING
Harmful laser light
The laser light source emits light at a power that may be harmful to the eyes.
➔Never look into the beam or direct it towards someone else.
➔System interlocks should never be disabled.

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Safety Information 1
Chemical and Biological Safety
Chemical and Biological Safety
To operate the instrument safely:
•Observe all cautionary information printed on the original solution
containers prior to their use.
•Because leaks, spills, or loss of sample may generate aerosols, observe
proper safety precautions.
•Agilent 2100 Bioanalyzer covers have not been designed as bioseals for
aerosol or liquid containment.
•Handle body fluids with care because they can transmit disease. No known
test offers complete assurance that they are free of micro-organisms. Some
of the most virulent – Hepatitis (B and C) and HIV (I-V) viruses, atypical
mycobacteria, and certain systemic fungi – further emphasize the need for
aerosol protection.
WARNING
Toxic and hazardous solvents
The handling of solvents and reagents can hold health risks.
➔When working with solvents observe appropriate safety procedures (for example,
goggles, safety gloves and protective clothing) as described in the material handling
and safety data sheet supplied by the solvent vendor, especially when toxic or
hazardous solvents are used.
WARNING
Pathogenic, toxic, or radioactive samples
Handling and use of pathogenic, toxic, or radioactive samples and of genetically
modified organisms holds risks for health and environment.
➔Ensure that all necessary safety regulations, guidelines, precautions and practices
are adhered to accordingly.
➔Consult the laboratory safety officer for advise on the level of containment required
for the application, and proper decontamination or sterilization procedures to follow
if fluids escape from containers.

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1Safety Information
Chemical and Biological Safety
•Always follow local state and federal biohazard handling regulation when
disposing of biohazardous waste material e.g. used Cell LabChips or
contaminated pressure adapters.
•Handle all infectious samples according to good laboratory procedures and
methods to prevent spread of disease.
•Dispose of all waste solutions and products according to appropriate
environmental health and safety guidelines.
WARNING
Harmful chemical and biological substances
Residues of clinical samples may contain chemicals and biological substances that
are dangerous and harmful to persons working on the 2100 Bioanalyzer.
➔The Agilent 2100 Bioanalyzer and any accessories must be decontaminated before
requesting service by an Agilent Technologies Field Service Representative and
before returning the 2100 Bioanalyzer to Agilent Technologies for repair or
replacement.

13
Safety Information 1
Legal Notice
Legal Notice
The Agilent 2100 Bioanalyzer is sold for research use only.
By purchasing this instrument, the purchaser is granted the limited right to
use only this instrument. Purchase of this instrument does not include any
right, express or implied, to use any other patented product, method or
process, or to use any other portion or component of any patented system or
systems, software, microfluidic devices or reagents, either alone or in
conjunction with this product, unless use of such method, process or other
portion or component is separately authorized.

14
1Safety Information
Legal Notice

15
Agilent Technologies
2
Site Requirements for the Agilent 2100
Bioanalyzer
Power Considerations 16
Bench space 17
Environment 18
Sound Emission 19
Physical Specifications of the Agilent 2100 Bioanalyzer 20
Two versions of the 2100 Bioanalyzer are available (see the label at the lower
right corner at the front of your 2100 Bioanalyzer). Both have the same
specifications and requirements except for the option to do flow cytometrie.
For both versions a suitable environment is important to ensure optimal
performance.
G2938C: for electrophoretic assays and for flow cytometrie
G2939A: for electrophoretic assays only

16
2Site Requirements for the Agilent 2100 Bioanalyzer
Power Considerations
Power Considerations
The Agilent 2100 Bioanalyzer power supply has wide ranging capabilities and
accepts any line voltage in the range 100 - 240 V ±10% with a line frequency of
50 - 60 Hz ±5% (see also Table 1 on page 20 for more details). Consequently,
there is no voltage selector in the rear of the instrument.
CAUTION
Damage through high line voltage
Connecting the Agilent 2100 Bioanalyzer to a line voltage higher than specified may
result in damage of the instrument.
➔Connect the Agilent 2100 Bioanalyzer to the specified line voltage only.

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Site Requirements for the Agilent 2100 Bioanalyzer 2
Bench space
Bench space
The Agilent 2100 Bioanalyzer dimensions and weight (see Table 1 on page 20)
allow you to place it on almost any desk or laboratory bench. It needs
approximately 8 cm (3.1 inches) of clear space at the rear for air circulation
and electric connections.

18
2Site Requirements for the Agilent 2100 Bioanalyzer
Environment
Environment
Your Agilent 2100 Bioanalyzer will work within the specifications of ambient
temperatures and relative humidity described in Table 1 on page 20.
CAUTION
Condensation
Condensation will damage the electronics.
➔Do not store, ship or use the instrument under conditions where temperature
fluctuations could cause condensation within the instrument.
➔If your instrument was shipped in cold weather, leave it in its box and allow it to
warm up slowly to room temperature to avoid condensation.
NOTE
The Agilent 2100 Bioanalyzer is designed to operate in a controlled electromagnetic
environment (EN61326/A1) where RF transmitters such as mobile telephones should not
be used in close proximity.
NOTE
In order to function properly, do not place the Agilent 2100 Bioanalyzer on a vibrating
surface or near vibrating objects.

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Site Requirements for the Agilent 2100 Bioanalyzer 2
Sound Emission
Sound Emission
Manufacturers Declaration
This statement is provided to comply with the requirements of the German
Sound Emission Directive of 18 January 1991.
This product has a sound pressure emission (at the operator position) < 70 dB.
•Sound Pressure Lp < 70 dB (A)
•At Operator Position
•Normal Operation
•According to ISO 7779:1988/EN 27779/1991 (Type Test)

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2Site Requirements for the Agilent 2100 Bioanalyzer
Physical Specifications of the Agilent 2100 Bioanalyzer
Physical Specifications of the Agilent 2100 Bioanalyzer
Table 1 on page 20 lists the physical specifications of the Agilent 2100
Bioanalyzer.
Table 1 Physical specifications
Type Specification Comment
Weight 10 kg (22 lbs)
Dimensions 162 — 412 — 290 mm
(6.4 — 16.2 — 11.4 in)
Width — depth — height
Line voltage 100-240 VAC ±10% Wide-ranging capability
Line frequency 50-60 Hz ±5%
Power consumption 30 W / 60 VA Maximum
Ambient operating temperature 5-40 °C (41-104 °F) The assays set-up to be analyzed on a chip
temperature of 30 °C. If the ambient
temperature is above 30 °C the assays are
analyzed using that temperature.
Ambient non-operating
temperature
-40-70 °C (-40-158 °F)
Humidity <80%, at 5-31 °C (41-89 °F), decreasing
linearly to 50% at 40 °C (104 °F)
Non-condensing
Operating altitude Up to 2000 m (6500 ft)
Non-operating altitude Up to 4600 m (14950 ft) For storage of the Agilent 2100 Bioanalyzer
Safety standards: IEC, CSA, UL Installation Category II, Pollution
Degree 2